[1]	NUCLEOTIDE SEQUENCE [MRNA], AND PARTIAL PROTEIN SEQUENCE. DOI=10.1021/bi00221a024; PubMed=1899621 [NCBI, ExPASy, EBI, Israel, Japan] Pilkington S.J., Skehel J.M., Walker J.E.; "The 30-kilodalton subunit of bovine mitochondrial complex I is homologous to a protein coded in chloroplast DNA."; Biochemistry 30:1901-1908(1991).
[2]	PROTEIN SEQUENCE OF 59-99 AND 197-266. TISSUE=Heart; PubMed=1907966 [NCBI, ExPASy, EBI, Israel, Japan] Masui R., Wakabayashi S., Matsubara H., Hatefi Y.; "The amino acid sequences of two 13 kDa polypeptides and partial amino acid sequence of 30 kDa polypeptide of complex I from bovine heart mitochondria: possible location of iron-sulfur clusters."; J. Biochem. 109:534-543(1991).

Comments

FUNCTION: Core subunit of the mitochondrial membrane respiratory chain NADH dehydrogenase (Complex I) that is believed to belong to the minimal assembly required for catalysis. Complex I functions in the transfer of electrons from NADH to the respiratory chain. The immediate electron acceptor for the enzyme is believed to be ubiquinone.
CATALYTIC ACTIVITY: NADH + ubiquinone = NAD⁺ + ubiquinol.
CATALYTIC ACTIVITY: NADH + acceptor = NAD⁺ + reduced acceptor.
SUBUNIT: Mammalian complex I is composed of 45 different subunits.
SUBCELLULAR LOCATION: Mitochondrion inner membrane.
SIMILARITY: Belongs to the complex I 30 kDa subunit family.

Copyright

Copyrighted by the UniProt Consortium, see http://www.uniprot.org/terms. Distributed under the Creative Commons Attribution-NoDerivs License.

Cross-references

Sequence databases

EMBL

M58469; AAA30663.1; ALT_SEQ; mRNA.

[EMBL / GenBank / DDBJ] [CoDingSequence]

A37957; A37957.

NP_777244.1; -.

3D structure databases

ModBase

P23709.

Ontologies

GO:0005747;	Cellular component: mitochondrial respiratory chain complex I (inferred from sequence or structural similarity from UniProtKB).
GO:0008137;	Molecular function: NADH dehydrogenase (ubiquinone) activity (inferred from electronic annotation from InterPro).
GO:0006917;	Biological process: induction of apoptosis (inferred from sequence or structural similarity from UniProtKB).
GO:0006120;	Biological process: mitochondrial electron transport, NADH to ubiquinone (inferred from electronic annotation from InterPro).
GO:0030308;	Biological process: negative regulation of cell growth (inferred from sequence or structural similarity from UniProtKB).
GO:0006800;	Biological process: oxygen and reactive oxygen species metabolic process (inferred from sequence or structural similarity from UniProtKB).
GO:0006810;	Biological process: transport (inferred from electronic annotation from UniProtKB-KW).
QuickGo view.

Family and domain databases

InterPro

IPR010218; NADH_DH_csu.
IPR001268; NADH_DHase_Ub_30kDa_su.
Graphical view of domain structure.

Pfam

PF00329; Complex1_30kDa; 1.
Pfam graphical view of domain structure.

ProDom

PD001581; Complex1_30K; 1.
[Domain structure / List of seq. sharing at least 1 domain]

TIGRFAMs

TIGR01961; NuoC_fam; 1.

PROSITE

PS00542; COMPLEX1_30K; 1.

Genome annotation databases

Ensembl

ENSBTAG00000018483; Bos taurus. [Contig view]

GeneID

287327; -.

KEGG

bta:287327; -.

Phylogenomic databases

HOVERGEN

P23709; -.

Other

UniRef

View cluster of proteins with at least 50% / 90% / 100% identity.

Keywords

Direct protein sequencing; Electron transport; Membrane; Mitochondrion; Mitochondrion inner membrane; NAD; Oxidoreductase; Respiratory chain; Transit peptide; Transport; Ubiquinone.

Features

Feature table viewer

`Key`	`From To`	`Length`	`Description`	`FTId`
`TRANSIT`	`1 38`	`38`	`Mitochondrion.`
`CHAIN`	`39 266`	`228`	`NADH dehydrogenase [ubiquinone] iron-sulfur protein 3, mitochondrial.`	`PRO_0000019997`
`CONFLICT`	`254 254`		`L -> E (in Ref. 2; AA sequence).`

Sequence information

Length: 266 AA [This is the length of the unprocessed precursor]

Molecular weight: 30284 Da [This is the MW of the unprocessed precursor]

CRC64: 43494B3359D2FEB1 [This is a checksum on the sequence]

        10         20         30         40         50         60 
MAAAVAAAAR GCWQRLVGSA APARVAGRPS VLLLPVRRES SAADTRPTVR PRNDVAHKQL 

        70         80         90        100        110        120 
SAFGEYVAEI LPKYVQQVQV SCFNELEICI HPDGVIPVLT FLRDHSNAQF KSLADLTAVD 

       130        140        150        160        170        180 
IPTRQNRFEI VYNLLSLRFN SRIRVKTYTD ELTPIESSVP VYKAANWYER EIWDMFGVFF 

       190        200        210        220        230        240 
ANHPDLRRIL TDYGFEGHPF RKDFPLSGYV ELRYDDEVKR VVAEPVELAQ EFRKFDLNSP 

       250        260 
WEAFPAYRQP PESLKLEAGD TKPEAK

P23709 in FASTA format

View entry in original UniProtKB/Swiss-Prot format
View entry in raw text format (no links)
Report form for errors/updates in this UniProtKB/Swiss-Prot entry

	BLAST submission on ExPASy/SIB or at NCBI (USA)		Sequence analysis tools: ProtParam, ProtScale, Compute pI/Mw, PeptideMass, PeptideCutter, Dotlet (Java)
	ScanProsite, MotifScan		Submit a homology modeling request to SWISS-MODEL
	NPSA Sequence analysis tools