[1]	NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM XB), AND PARTIAL PROTEIN SEQUENCE. TISSUE=Erythrocyte; PubMed=2137451 [NCBI, ExPASy, EBI, Israel, Japan] Strehler E.E., James P., Fischer R., Heim R., Vorherr T.E., Filoteo A.G., Penniston J.T., Carafoli E.; "Peptide sequence analysis and molecular cloning reveal two calcium pump isoforms in the human erythrocyte membrane."; J. Biol. Chem. 265:2835-2842(1990).
[2]	NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM XA). TISSUE=Fetal brain; PubMed=1531651 [NCBI, ExPASy, EBI, Israel, Japan] Brandt P., Neve R.L., Kammesheidt A., Rhoads R.E., Vanaman T.C.; "Analysis of the tissue-specific distribution of mRNAs encoding the plasma membrane calcium-pumping ATPases and characterization of an alternately spliced form of PMCA4 at the cDNA and genomic levels."; J. Biol. Chem. 267:4376-4385(1992).
[3]	PARTIAL NUCLEOTIDE SEQUENCE (ISOFORMS A; B; D AND K). TISSUE=Heart muscle; DOI=10.1007/BF00229314; PubMed=8700162 [NCBI, ExPASy, EBI, Israel, Japan] Santiago-Garcia J., Mas-Oliva J., Saavedra D., Zarain-Herzberg A.; "Analysis of mRNA expression and cloning of a novel plasma membrane Ca(2+)-ATPase splice variant in human heart."; Mol. Cell. Biochem. 155:173-182(1996).
[4]	PROTEIN SEQUENCE OF 1085-1153 (ISOFORMS XB/ZB), AND CALMODULIN-BINDING SUBDOMAIN A. PubMed=2963820 [NCBI, ExPASy, EBI, Israel, Japan] James P., Maeda M., Fischer R., Verma A.K., Krebs J., Penniston J.T., Carafoli E.; "Identification and primary structure of a calmodulin binding domain of the Ca2+ pump of human erythrocytes."; J. Biol. Chem. 263:2905-2910(1988).
[5]	PROTEIN SEQUENCE OF 1177-1190. PubMed=2966397 [NCBI, ExPASy, EBI, Israel, Japan] Brandt P., Zurini M., Neve R.L., Rhoads R.E., Vanaman T.C.; "A C-terminal, calmodulin-like regulatory domain from the plasma membrane Ca2+-pumping ATPase."; Proc. Natl. Acad. Sci. U.S.A. 85:2914-2918(1988).
[6]	ALTERNATIVE SPLICING (ISOFORMS X AND Z). TISSUE=Heart; PubMed=8245032 [NCBI, ExPASy, EBI, Israel, Japan] Stauffer T.P., Hilfiker H., Carafoli E., Strehler E.E.; "Quantitative analysis of alternative splicing options of human plasma membrane calcium pump genes."; J. Biol. Chem. 268:25993-26003(1993).
[7]	ERRATUM. PubMed=7989379 [NCBI, ExPASy, EBI, Israel, Japan] Stauffer T.P., Hilfiker H., Carafoli E., Strehler E.E.; J. Biol. Chem. 269:32022-32022(1994).
[8]	STRUCTURE BY NMR OF 1086-1104 IN COMPLEX WITH CALMODULIN. DOI=10.1021/bi9908235; PubMed=10493800 [NCBI, ExPASy, EBI, Israel, Japan] Elshorst B., Hennig M., Foersterling H., Diener A., Maurer M., Schulte P., Schwalbe H., Griesinger C., Krebs J., Schmid H., Vorherr T.E., Carafoli E.; "NMR solution structure of a complex of calmodulin with a binding peptide of the Ca(2+) pump."; Biochemistry 38:12320-12332(1999).

Comments

FUNCTION: This magnesium-dependent enzyme catalyzes the hydrolysis of ATP coupled with the transport of calcium out of the cell.
CATALYTIC ACTIVITY: ATP + H₂O + Ca²⁺(Cis) = ADP + phosphate + Ca²⁺(Trans).
INTERACTION:
Q62696:Dlg1 (xeno); NbExp=1; IntAct=EBI-1174437, EBI-389325;
Q63622:Dlg2 (xeno); NbExp=2; IntAct=EBI-1174437, EBI-396947;
Q62936:Dlg3 (xeno); NbExp=2; IntAct=EBI-1174437, EBI-349596;
P31016:Dlg4 (xeno); NbExp=1; IntAct=EBI-1174437, EBI-375655;
SUBCELLULAR LOCATION: Cell membrane; Multi-pass membrane protein.

ALTERNATIVE PRODUCTS: 8 named isoforms [FASTA] produced by alternative splicing. There is a combination of two alternatively spliced domains at N-terminal site A (X and Z) and at C-terminal site B/C (A, B, D and K). The splice sites have mostly been studied independently. Full isoforms so far detected are isoform XA and isoform XB. Experimental confirmation may be lacking for some isoforms.

Name	XD
Synonyms	AIICIV
Isoform ID	P23634-1
This is the isoform sequence displayed in this entry.

Name	XA
Synonyms	AIICII
Isoform ID	P23634-2
Features which should be applied to build the isoform sequence: VSP_000405.

Name	ZA
Synonyms	AICII
Isoform ID	P23634-3
Features which should be applied to build the isoform sequence: VSP_000402, VSP_000405.

Name	XK
Synonyms	XG
Isoform ID	P23634-4
Features which should be applied to build the isoform sequence: VSP_000403, VSP_000405.

Name	ZK
Synonyms	ZG
Isoform ID	P23634-5
Features which should be applied to build the isoform sequence: VSP_000402, VSP_000403, VSP_000405.

Name	XB
Synonyms	AIICI
Isoform ID	P23634-6
Features which should be applied to build the isoform sequence: VSP_000404.

Name	ZB
Synonyms	AICI
Isoform ID	P23634-7
Features which should be applied to build the isoform sequence: VSP_000402, VSP_000404.

Name	ZD
Synonyms	AICIV
Isoform ID	P23634-8
Features which should be applied to build the isoform sequence: VSP_000402.

TISSUE SPECIFICITY: Isoform XB is the most abundant isoform and is expressed ubiquitously. Isoforms containing segment Z have only been detected in heart, while isoforms containing segment a have been found in heart, stomach and brain cortex.
SIMILARITY: Belongs to the cation transport ATPase (P-type) family. Type IIB subfamily.

Copyright

Copyrighted by the UniProt Consortium, see http://www.uniprot.org/terms. Distributed under the Creative Commons Attribution-NoDerivs License.

Cross-references

Sequence databases

EMBL

M25874; AAA50819.1; -; mRNA.	[EMBL / GenBank / DDBJ] [CoDingSequence]
M83363; AAA36455.1; -; mRNA.	[EMBL / GenBank / DDBJ] [CoDingSequence]
U42026; AAB17577.1; -; mRNA.	[EMBL / GenBank / DDBJ] [CoDingSequence]
U42061; AAB17578.1; -; mRNA.	[EMBL / GenBank / DDBJ] [CoDingSequence]
U42062; AAB17579.1; -; mRNA.	[EMBL / GenBank / DDBJ] [CoDingSequence]
U42378; AAB17580.1; -; mRNA.	[EMBL / GenBank / DDBJ] [CoDingSequence]

PIR

A35547; A35547.

UniGene

Hs.343522

3D structure databases

PDB

1CFF; NMR; -; B=1086-1104.

[ExPASy / RCSB / EBI]

PDBsum

1CFF; -.

ModBase

P23634.

Protein-protein interaction databases

DIP

DIP:6128N; -.

IntAct

P23634; -.

PTM databases

PhosphoSite

P23634; -.

Organism-specific databases

HIX0023651; -.

HGNC:817; ATP2B4.

CAB016118; -.

108732; gene. [NCBI / EBI]

PharmGKB

PA25110; -.

GeneCards

P23634.

Gene expression databases

ArrayExpress

P23634; -.

CleanEx

HS_ATP2B2; -.
HS_ATP2B4; -.

GermOnline

ENSG00000058668; Homo sapiens.

Ontologies

GO:0005887;	Cellular component: integral to plasma membrane (traceable author statement from ProtInc).
GO:0005524;	Molecular function: ATP binding (inferred from electronic annotation from InterPro).
GO:0005509;	Molecular function: calcium ion binding (inferred from electronic annotation from InterPro).
GO:0005388;	Molecular function: calcium-transporting ATPase activity (traceable author statement from ProtInc).
GO:0005516;	Molecular function: calmodulin binding (inferred from electronic annotation from UniProtKB-KW).
GO:0000287;	Molecular function: magnesium ion binding (inferred from electronic annotation from UniProtKB-KW).
GO:0006816;	Biological process: calcium ion transport (inferred from electronic annotation from InterPro).
GO:0008152;	Biological process: metabolic process (inferred from electronic annotation from InterPro).
QuickGo view.

Family and domain databases

InterPro

IPR006408; ATPase-IIB_Ca.
IPR001757; ATPase_P.
IPR006068; ATPase_P_cat_C.
IPR004014; ATPase_P_cat_N.
IPR005834; Dehalogen-like_hydro.
IPR008250; E1-E2_ATPase_reg.
Graphical view of domain structure.

PANTHER

PTHR11939; ATPase_P; 1.

Pfam

PF00689; Cation_ATPase_C; 1.
PF00690; Cation_ATPase_N; 1.
PF00122; E1-E2_ATPase; 1.
PF00702; Hydrolase; 1.
Pfam graphical view of domain structure.

PRINTS

PR00119; CATATPASE.

TIGRFAMs

TIGR01517; ATPase-IIB_Ca; 1.
TIGR01494; ATPase_P-type; 4.

PROSITE

PS00154; ATPASE_E1_E2; 1.

ProtoNet

P23634.

Genome annotation databases

Ensembl

ENSG00000058668; Homo sapiens. [Contig view]

Phylogenomic databases

HOGENOM

P23634; -.

HOVERGEN

P23634; -.

Other

LinkHub

P23634; -.

SOURCE

ATP2B4; Homo sapiens.

UniRef

View cluster of proteins with at least 50% / 90% / 100% identity.

Keywords

3D-structure; Alternative splicing; ATP-binding; Calcium; Calcium transport; Calmodulin-binding; Cell membrane; Direct protein sequencing; Hydrolase; Ion transport; Magnesium; Membrane; Metal-binding; Nucleotide-binding; Phosphoprotein; Transmembrane; Transport.

Features

Feature table viewer

Feature aligner

`Key`	`From To`	`Length`	`Description`	`FTId`
`CHAIN`	`1 1241`	`1241`	`Plasma membrane calcium-transporting ATPase 4.`	`PRO_0000046220`
`TOPO_DOM`	`1 92`	`92`	`Cytoplasmic (Potential).`
`TRANSMEM`	`93 113`	`21`	`Potential.`
`TOPO_DOM`	`114 150`	`37`	`Extracellular (Potential).`
`TRANSMEM`	`151 171`	`21`	`Potential.`
`TOPO_DOM`	`172 356`	`185`	`Cytoplasmic (Potential).`
`TRANSMEM`	`357 376`	`20`	`Potential.`
`TOPO_DOM`	`377 409`	`33`	`Extracellular (Potential).`
`TRANSMEM`	`410 427`	`18`	`Potential.`
`TOPO_DOM`	`428 840`	`413`	`Cytoplasmic (Potential).`
`TRANSMEM`	`841 860`	`20`	`Potential.`
`TOPO_DOM`	`861 870`	`10`	`Extracellular (Potential).`
`TRANSMEM`	`871 891`	`21`	`Potential.`
`TOPO_DOM`	`892 911`	`20`	`Cytoplasmic (Potential).`
`TRANSMEM`	`912 934`	`23`	`Potential.`
`TOPO_DOM`	`935 952`	`18`	`Extracellular (Potential).`
`TRANSMEM`	`953 974`	`22`	`Potential.`
`TOPO_DOM`	`975 993`	`19`	`Cytoplasmic (Potential).`
`TRANSMEM`	`994 1015`	`22`	`Potential.`
`TOPO_DOM`	`1016 1025`	`10`	`Extracellular (Potential).`
`TRANSMEM`	`1026 1047`	`22`	`Potential.`
`TOPO_DOM`	`1048 1241`	`194`	`Cytoplasmic (Potential).`
`REGION`	`1086 1103`	`18`	`Calmodulin-binding subdomain A.`
`REGION`	`1104 1113`	`10`	`Calmodulin-binding subdomain B (By similarity).`
`COMPBIAS`	`297 303`	`7`	`Poly-Lys.`
`COMPBIAS`	`1186 1190`	`5`	`Poly-Glu.`
`ACT_SITE`	`465 465`		`4-aspartylphosphate intermediate (By similarity).`
`METAL`	`785 785`		`Magnesium (By similarity).`
`METAL`	`789 789`		`Magnesium (By similarity).`
`MOD_RES`	`1102 1102`		`Phosphothreonine; by PKC (By similarity).`
`VAR_SEQ`	`301 312`		`Missing (in isoform ZA, isoform ZK, isoform ZB and isoform ZD).`	`VSP_000402`
`VAR_SEQ`	`1009 1044`		`Missing (in isoform XK and isoform ZK).`	`VSP_000403`
`VAR_SEQ`	`1104 1139`		`Missing (in isoform XB and isoform ZB).`	`VSP_000404`
`VAR_SEQ`	`1140 1241`		`IKVVKAFHSSLHESIQKPYNQKSIHSFMTHPEFAIEEELP` `RTPLLDEEEEENPDKASKFGTRVLLLDGEVTPYANTNNNA` `VDCNQVQLPQSDSSLQSLETSV -> VAVAPVKSSPTTSVPAVSSPPMGNQSGQSVP (in isoform XA, isoform XK, isoform ZA and isoform ZK).`	`VSP_000405`
`CONFLICT`	`1144 1144`		`K -> N (in Ref. 4; AA sequence).`
`CONFLICT`	`1147 1147`		`H -> S (in Ref. 4; AA sequence).`
`CONFLICT`	`1153 1153`		`S -> F (in Ref. 4; AA sequence).`
`CONFLICT`	`1178 1178`		`L -> Q (in Ref. 5; AA sequence).`
`CONFLICT`	`1187 1187`		`E -> Q (in Ref. 5; AA sequence).`
`TURN`	`1090 1092`	`3`
`HELIX`	`1093 1102`	`10`

Sequence information

Length: 1241 AA [This is the length of the unprocessed precursor]

Molecular weight: 137920 Da [This is the MW of the unprocessed precursor]

CRC64: 568544103CD5F494 [This is a checksum on the sequence]

        10         20         30         40         50         60 
MTNPSDRVLP ANSMAESREG DFGCTVMELR KLMELRSRDA LTQINVHYGG VQNLCSRLKT 

        70         80         90        100        110        120 
SPVEGLSGNP ADLEKRRQVF GHNVIPPKKP KTFLELVWEA LQDVTLIILE IAAIISLVLS 

       130        140        150        160        170        180 
FYRPAGEENE LCGQVATTPE DENEAQAGWI EGAAILFSVI IVVLVTAFND WSKEKQFRGL 

       190        200        210        220        230        240 
QCRIEQEQKF SIIRNGQLIQ LPVAEIVVGD IAQVKYGDLL PADGILIQGN DLKIDESSLT 

       250        260        270        280        290        300 
GESDHVKKSL DKDPMLLSGT HVMEGSGRMV VTAVGVNSQT GIILTLLGVN EDDEGEKKKK 

       310        320        330        340        350        360 
GKKQGVPENR NKAKTQDGVA LEIQPLNSQE GIDNEEKDKK AVKVPKKEKS VLQGKLTRLA 

       370        380        390        400        410        420 
VQIGKAGLLM SALTVFILIL YFVIDNFVIN RRPWLPECTP IYIQYFVKFF IIGITVLVVA 

       430        440        450        460        470        480 
VPEGLPLAVT ISLAYSVKKM MKDNNLVRHL DACETMGNAT AICSDKTGTL TMNRMTVVQA 

       490        500        510        520        530        540 
YIGGIHYRQI PSPDVFLPKV LDLIVNGISI NSAYTSKILP PEKEGGLPRQ VGNKTECALL 

       550        560        570        580        590        600 
GFVTDLKQDY QAVRNEVPEE KLYKVYTFNS VRKSMSTVIR NPNGGFRMYS KGASEIILRK 

       610        620        630        640        650        660 
CNRILDRKGE AVPFKNKDRD DMVRTVIEPM ACDGLRTICI AYRDFDDTEP SWDNENEILT 

       670        680        690        700        710        720 
ELTCIAVVGI EDPVRPEVPD AIAKCKQAGI TVRMVTGDNI NTARAIATKC GILTPGDDFL 

       730        740        750        760        770        780 
CLEGKEFNRL IRNEKGEVEQ EKLDKIWPKL RVLARSSPTD KHTLVKGIID STVGEHRQVV 

       790        800        810        820        830        840 
AVTGDGTNDG PALKKADVGF AMGIAGTDVA KEASDIILTD DNFTSIVKAV MWGRNVYDSI 

       850        860        870        880        890        900 
SKFLQFQLTV NVVAVIVAFT GACITQDSPL KAVQMLWVNL IMDTFASLAL ATEPPTESLL 

       910        920        930        940        950        960 
KRRPYGRNKP LISRTMMKNI LGHAFYQLIV IFILVFAGEK FFDIDSGRKA PLHSPPSQHY 

       970        980        990       1000       1010       1020 
TIVFNTFVLM QLFNEINSRK IHGEKNVFSG IYRNIIFCSV VLGTFICQIF IVEFGGKPFS 

      1030       1040       1050       1060       1070       1080 
CTSLSLSQWL WCLFIGIGEL LWGQFISAIP TRSLKFLKEA GHGTTKEEIT KDAEGLDEID 

      1090       1100       1110       1120       1130       1140 
HAEMELRRGQ ILWFRGLNRI QTQIDVINTF QTGASFKGVL RRQNMGQHLD VKLVPSSSYI 

      1150       1160       1170       1180       1190       1200 
KVVKAFHSSL HESIQKPYNQ KSIHSFMTHP EFAIEEELPR TPLLDEEEEE NPDKASKFGT 

      1210       1220       1230       1240 
RVLLLDGEVT PYANTNNNAV DCNQVQLPQS DSSLQSLETS V

P23634 in FASTA format

View entry in original UniProtKB/Swiss-Prot format
View entry in raw text format (no links)
Report form for errors/updates in this UniProtKB/Swiss-Prot entry

	BLAST submission on ExPASy/SIB or at NCBI (USA)		Sequence analysis tools: ProtParam, ProtScale, Compute pI/Mw, PeptideMass, PeptideCutter, Dotlet (Java)
	ScanProsite, MotifScan		Submit a homology modeling request to SWISS-MODEL
	NPSA Sequence analysis tools