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UniProtKB/Swiss-Prot entry P23572


[Entry info] [Name and origin] [References] [Comments] [Cross-references] [Keywords] [Features] [Sequence] [Tools]

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Entry information
Entry name CDC2_DROME
Primary accession number P23572
Secondary accession numbers A0ANT1 Q86FT5 Q86FT6 Q86FT7 Q86FT8 Q86FT9 Q9TX68 Q9TX69 Q9TX70 Q9TX71 Q9TX72 Q9TX73 Q9TX74 Q9VKX5
Integrated into Swiss-Prot on November 1, 1991
Sequence was last modified on November 1, 1991 (Sequence version 1)
Annotations were last modified on    November 25, 2008 (Entry version 103)
Name and origin of the protein
Protein name Cell division control protein 2 homolog
Synonyms EC 2.7.11.22
EC 2.7.11.23
p34 protein kinase
Gene name
Name: cdc2
ORFNames: CG5363
From
Drosophila melanogaster (Fruit fly) [TaxID: 7227] 
Taxonomy Eukaryota; Metazoa; Arthropoda; Hexapoda; Insecta; Pterygota; Neoptera; Endopterygota; Diptera; Brachycera; Muscomorpha; Ephydroidea; Drosophilidae; Drosophila; Sophophora.
Protein existence 1: Evidence at protein level;
References
[1]
NUCLEOTIDE SEQUENCE [MRNA].
PubMed=2120045 [NCBI, ExPASy, EBI, Israel, Japan]
Lehner C.F., O'Farrell P.H.;
"Drosophila cdc2 homologs: a functional homolog is coexpressed with a cognate variant.";
EMBO J. 9:3573-3581(1990).
[2]
NUCLEOTIDE SEQUENCE [MRNA], FUNCTION, AND DEVELOPMENTAL STAGE.
PubMed=2120044 [NCBI, ExPASy, EBI, Israel, Japan]
Jimenez J., Alphey L., Nurse P., Glover D.M.;
"Complementation of fission yeast cdc2ts and cdc25ts mutants identifies two cell cycle genes from Drosophila: a cdc2 homologue and string.";
EMBO J. 9:3565-3571(1990).
[3]
NUCLEOTIDE SEQUENCE [GENOMIC DNA / MRNA], AND MUTAGENESIS OF ALA-145; GLY-148; GLU-196; GLY-206 AND PRO-242.
PubMed=8223248 [NCBI, ExPASy, EBI, Israel, Japan]
Stern B., Ried G., Clegg N.J., Grigliatti T.A., Lehner C.F.;
"Genetic analysis of the Drosophila cdc2 homolog.";
Development 117:219-232(1993).
[4]
NUCLEOTIDE SEQUENCE [GENOMIC DNA].
STRAIN=ZBMEL131, ZBMEL145, ZBMEL157, ZBMEL186, ZBMEL229, ZBMEL377, ZBMEL384, ZBMEL398, ZBMEL82, ZBMEL84, and ZBMEL95;
DOI=10.1534/genetics.106.058008; PubMed=16951084 [NCBI, ExPASy, EBI, Israel, Japan]
Proeschel M., Zhang Z., Parsch J.;
"Widespread adaptive evolution of Drosophila genes with sex-biased expression.";
Genetics 174:893-900(2006).
[5]
NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
STRAIN=Berkeley;
DOI=10.1126/science.287.5461.2185; PubMed=10731132 [NCBI, ExPASy, EBI, Israel, Japan]
Adams M.D., Celniker S.E., Holt R.A., Evans C.A., Gocayne J.D., Amanatides P.G., Scherer S.E., Li P.W., Hoskins R.A., Galle R.F., George R.A., Lewis S.E., Richards S., Ashburner M., Henderson S.N., Sutton G.G., Wortman J.R., Yandell M.D., Zhang Q., Chen L.X., Brandon R.C., Rogers Y.-H.C., Blazej R.G., Champe M., Pfeiffer B.D., Wan K.H., Doyle C., Baxter E.G., Helt G., Nelson C.R., Miklos G.L.G., Abril J.F., Agbayani A., An H.-J., Andrews-Pfannkoch C., Baldwin D., Ballew R.M., Basu A., Baxendale J., Bayraktaroglu L., Beasley E.M., Beeson K.Y., Benos P.V., Berman B.P., Bhandari D., Bolshakov S., Borkova D., Botchan M.R., Bouck J., Brokstein P., Brottier P., Burtis K.C., Busam D.A., Butler H., Cadieu E., Center A., Chandra I., Cherry J.M., Cawley S., Dahlke C., Davenport L.B., Davies P., de Pablos B., Delcher A., Deng Z., Mays A.D., Dew I., Dietz S.M., Dodson K., Doup L.E., Downes M., Dugan-Rocha S., Dunkov B.C., Dunn P., Durbin K.J., Evangelista C.C., Ferraz C., Ferriera S., Fleischmann W., Fosler C., Gabrielian A.E., Garg N.S., Gelbart W.M., Glasser K., Glodek A., Gong F., Gorrell J.H., Gu Z., Guan P., Harris M., Harris N.L., Harvey D.A., Heiman T.J., Hernandez J.R., Houck J., Hostin D., Houston K.A., Howland T.J., Wei M.-H., Ibegwam C., Jalali M., Kalush F., Karpen G.H., Ke Z., Kennison J.A., Ketchum K.A., Kimmel B.E., Kodira C.D., Kraft C.L., Kravitz S., Kulp D., Lai Z., Lasko P., Lei Y., Levitsky A.A., Li J.H., Li Z., Liang Y., Lin X., Liu X., Mattei B., McIntosh T.C., McLeod M.P., McPherson D., Merkulov G., Milshina N.V., Mobarry C., Morris J., Moshrefi A., Mount S.M., Moy M., Murphy B., Murphy L., Muzny D.M., Nelson D.L., Nelson D.R., Nelson K.A., Nixon K., Nusskern D.R., Pacleb J.M., Palazzolo M., Pittman G.S., Pan S., Pollard J., Puri V., Reese M.G., Reinert K., Remington K., Saunders R.D.C., Scheeler F., Shen H., Shue B.C., Siden-Kiamos I., Simpson M., Skupski M.P., Smith T.J., Spier E., Spradling A.C., Stapleton M., Strong R., Sun E., Svirskas R., Tector C., Turner R., Venter E., Wang A.H., Wang X., Wang Z.-Y., Wassarman D.A., Weinstock G.M., Weissenbach J., Williams S.M., Woodage T., Worley K.C., Wu D., Yang S., Yao Q.A., Ye J., Yeh R.-F., Zaveri J.S., Zhan M., Zhang G., Zhao Q., Zheng L., Zheng X.H., Zhong F.N., Zhong W., Zhou X., Zhu S.C., Zhu X., Smith H.O., Gibbs R.A., Myers E.W., Rubin G.M., Venter J.C.;
"The genome sequence of Drosophila melanogaster.";
Science 287:2185-2195(2000).
[6]
GENOME REANNOTATION.
PubMed=12537572 [NCBI, ExPASy, EBI, Israel, Japan]
Misra S., Crosby M.A., Mungall C.J., Matthews B.B., Campbell K.S., Hradecky P., Huang Y., Kaminker J.S., Millburn G.H., Prochnik S.E., Smith C.D., Tupy J.L., Whitfield E.J., Bayraktaroglu L., Berman B.P., Bettencourt B.R., Celniker S.E., de Grey A.D.N.J., Drysdale R.A., Harris N.L., Richter J., Russo S., Schroeder A.J., Shu S.Q., Stapleton M., Yamada C., Ashburner M., Gelbart W.M., Rubin G.M., Lewis S.E.;
"Annotation of the Drosophila melanogaster euchromatic genome: a systematic review.";
Genome Biol. 3:RESEARCH0083.1-RESEARCH0083.22(2002).
[7]
NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
STRAIN=Berkeley;
PubMed=12537569 [NCBI, ExPASy, EBI, Israel, Japan]
Stapleton M., Carlson J.W., Brokstein P., Yu C., Champe M., George R.A., Guarin H., Kronmiller B., Pacleb J.M., Park S., Wan K.H., Rubin G.M., Celniker S.E.;
"A Drosophila full-length cDNA resource.";
Genome Biol. 3:RESEARCH0080.1-RESEARCH0080.8(2002).
[8]
MUTAGENESIS OF GLY-43; ALA-145; GLY-148; LEU-176; GLU-196; GLY-206 AND PRO-242, AND DEVELOPMENTAL STAGE.
PubMed=8405984 [NCBI, ExPASy, EBI, Israel, Japan]
Clegg N.J., Whitehead I.P., Williams J.A., Spiegelman G.B., Grigliatti T.A.;
"A developmental and molecular analysis of cdc2 mutations in Drosophila melanogaster.";
Genome 36:676-685(1993).
[9]
PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT THR-14; TYR-15; TYR-160 AND THR-161, AND MASS SPECTROMETRY.
TISSUE=Embryo;
DOI=10.1021/pr700696a; PubMed=18327897 [NCBI, ExPASy, EBI, Israel, Japan]
Zhai B., Villen J., Beausoleil S.A., Mintseris J., Gygi S.P.;
"Phosphoproteome analysis of Drosophila melanogaster embryos.";
J. Proteome Res. 7:1675-1682(2008).
Comments
Copyright
Copyrighted by the UniProt Consortium, see http://www.uniprot.org/terms. Distributed under the Creative Commons Attribution-NoDerivs License.
Cross-references
Sequence databases
EMBL
X57485; CAA40723.1; -; mRNA.[EMBL / GenBank / DDBJ] [CoDingSequence]
X57496; CAA40733.1; -; mRNA.[EMBL / GenBank / DDBJ] [CoDingSequence]
S66801; AAP13986.1; -; Genomic_DNA.[EMBL / GenBank / DDBJ] [CoDingSequence]
S66804; AAP13987.1; -; Genomic_DNA.[EMBL / GenBank / DDBJ] [CoDingSequence]
S66805; AAP13988.1; -; Genomic_DNA.[EMBL / GenBank / DDBJ] [CoDingSequence]
S66807; AAP13989.1; -; Genomic_DNA.[EMBL / GenBank / DDBJ] [CoDingSequence]
S66810; AAP13990.1; -; Genomic_DNA.[EMBL / GenBank / DDBJ] [CoDingSequence]
AM294319; CAL26249.1; -; Genomic_DNA.[EMBL / GenBank / DDBJ] [CoDingSequence]
AM294320; CAL26250.1; -; Genomic_DNA.[EMBL / GenBank / DDBJ] [CoDingSequence]
AM294321; CAL26251.1; -; Genomic_DNA.[EMBL / GenBank / DDBJ] [CoDingSequence]
AM294322; CAL26252.1; -; Genomic_DNA.[EMBL / GenBank / DDBJ] [CoDingSequence]
AM294323; CAL26253.1; -; Genomic_DNA.[EMBL / GenBank / DDBJ] [CoDingSequence]
AM294324; CAL26254.1; -; Genomic_DNA.[EMBL / GenBank / DDBJ] [CoDingSequence]
AM294325; CAL26255.1; -; Genomic_DNA.[EMBL / GenBank / DDBJ] [CoDingSequence]
AM294326; CAL26256.1; -; Genomic_DNA.[EMBL / GenBank / DDBJ] [CoDingSequence]
AM294327; CAL26257.1; -; Genomic_DNA.[EMBL / GenBank / DDBJ] [CoDingSequence]
AM294328; CAL26258.1; -; Genomic_DNA.[EMBL / GenBank / DDBJ] [CoDingSequence]
AM294329; CAL26259.1; -; Genomic_DNA.[EMBL / GenBank / DDBJ] [CoDingSequence]
AE014134; AAF52932.1; -; Genomic_DNA.[EMBL / GenBank / DDBJ] [CoDingSequence]
AY061450; AAL28998.1; -; mRNA.[EMBL / GenBank / DDBJ] [CoDingSequence]
PIR S12009; S12009.
RefSeq NP_476797.1; -.
UniGene Dm.3187
3D structure databases
HSSP P24941; 1HCL. [HSSP ENTRY / PDB]
ModBase P23572.
Protein-protein interaction databases
IntAct P23572; -.
Enzyme and pathway databases
BioCyc DMEL-XXX-02:DMEL-XXX-02-008371-MON; -.
Organism-specific databases
FlyBase FBgn0004106; cdc2.
Gene expression databases
ArrayExpress P23572; -.
GermOnline CG5363; Drosophila melanogaster.
Ontologies
GO
GO:0005634; Cellular component: nucleus (inferred from electronic annotation from UniProtKB-KW).
GO:0005524; Molecular function: ATP binding (inferred from electronic annotation from InterPro).
GO:0004693; Molecular function: cyclin-dependent protein kinase activity (inferred from electronic annotation from EC).
GO:0005515; Molecular function: protein binding (inferred from physical interaction from UniProtKB).
GO:0008353; Molecular function: RNA polymerase subunit kinase activity (inferred from electronic annotation from EC).
GO:0055059; Biological process: asymmetric neuroblast division (inferred from mutant phenotype from FlyBase).
GO:0000086; Biological process: G2/M transition of mitotic cell cycle (inferred from mutant phenotype from FlyBase).
GO:0048142; Biological process: germarium-derived cystoblast division (inferred from mutant phenotype from FlyBase).
GO:0007141; Biological process: male meiosis I (traceable author statement from FlyBase).
GO:0008315; Biological process: meiotic G2/MI transition (traceable author statement from FlyBase).
GO:0007067; Biological process: mitosis (inferred from electronic annotation from UniProtKB-KW).
GO:0006468; Biological process: protein amino acid phosphorylation (inferred from electronic annotation from InterPro).
GO:0007284; Biological process: spermatogonial cell division (inferred from mutant phenotype from FlyBase).
QuickGo view.
Family and domain databases
InterPro IPR000719; Prot_kinase_core.
IPR017441; Protein_kinase_ATP_bd_CS.
IPR017442; Se/Thr_pkinase-rel.
IPR008271; Ser_thr_pkin_AS.
IPR002290; Ser_thr_pkinase.
Graphical view of domain structure.
Pfam PF00069; Pkinase; 1.
Pfam graphical view of domain structure.
ProDom PD000001; Prot_kinase; 1.
[Domain structure / List of seq. sharing at least 1 domain]
SMART SM00220; S_TKc; 1.
SMART graphical view of domain structure.
PROSITE PS00107; PROTEIN_KINASE_ATP; 1.
PS50011; PROTEIN_KINASE_DOM; 1.
PS00108; PROTEIN_KINASE_ST; 1.
PROSITE graphical view of domain structure (profiles).
ProtoNet P23572.
Genome annotation databases
Ensembl CG5363; Drosophila melanogaster. [Contig view]
GeneID 34411; -.
KEGG dme:Dmel_CG5363; -.
NMPDR fig|7227.3.peg.1811; -.
Other
NextBio 788369; -.
UniRef View cluster of proteins with at least 50% / 90% / 100% identity.
Keywords
ATP-binding; Cell cycle; Cell division; Complete proteome; Kinase; Mitosis; Nucleotide-binding; Nucleus; Phosphoprotein; Serine/threonine-protein kinase; Transferase.
Features
SEVIEWER logo Feature table viewer FT aligner logo Feature aligner
KeyFrom   To Length Description FTId
CHAIN   1   297  297     Cell division control protein 2 homolog. PRO_0000085743
DOMAIN   4   287  284     Protein kinase. 
NP_BIND   10    18  9     ATP (By similarity). 
ACT_SITE   128   128        Proton acceptor (By similarity). 
BINDING   33    33        ATP (By similarity). 
MOD_RES   14    14        Phosphothreonine. 
MOD_RES   15    15        Phosphotyrosine. 
MOD_RES   160   160        Phosphotyrosine. 
MOD_RES   161   161        Phosphothreonine; by CAK (Potential). 
MUTAGEN   43    43        G->D: In cdc2-E1-4; larval-pupal lethal with some adult escapers. 
MUTAGEN   145   145        A->V: In cdc2-216A; larval-pupal lethal. 
MUTAGEN   148   148        G->R: In cdc2-D57; embryonic or larval-pupal lethal. 
MUTAGEN   176   176        L->Q: In cdc2-E10; larval-pupal lethal. 
MUTAGEN   196   196        E->K: In cdc2-E1-24; larval-pupal lethal with some adult escapers. 
MUTAGEN   206   206        G->D: In cdc2-E1-23; larval-pupal lethal. 
MUTAGEN   242   242        P->S: In cdc2-E1-9; larval-pupal lethal. 
Sequence information
Length: 297 AA [This is the length of the unprocessed precursor] Molecular weight: 34439 Da [This is the MW of the unprocessed precursor] CRC64: 952CF136D8AB64C0 [This is a checksum on the sequence]
        10         20         30         40         50         60 
MEDFEKIEKI GEGTYGVVYK GRNRLTGQIV AMKKIRLESD DEGVPSTAIR EISLLKELKH 

        70         80         90        100        110        120 
ENIVCLEDVL MEENRIYLIF EFLSMDLKKY MDSLPVDKHM ESELVRSYLY QITSAILFCH 

       130        140        150        160        170        180 
RRRVLHRDLK PQNLLIDKSG LIKVADFGLG RSFGIPVRIY THEIVTLWYR APEVLLGSPR 

       190        200        210        220        230        240 
YSCPVDIWSI GCIFAEMATR KPLFQGDSEI DQLFRMFRIL KTPTEDIWPG VTSLPDYKNT 

       250        260        270        280        290 
FPCWSTNQLT NQLKNLDANG IDLIQKMLIY DPVHRISAKD ILEHPYFNGF QSGLVRN 

P23572 in FASTA format

View entry in original UniProtKB/Swiss-Prot format
View entry in raw text format (no links)
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Tools Sequence analysis tools: ProtParam, ProtScale, Compute pI/Mw, PeptideMass, PeptideCutter, Dotlet (Java)
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