Lactose permease IIC component
     (PTS system lactose-specific EIIC component)
Lactose-specific phosphotransferase enzyme IIB component
     (EC 2.7.1.69)
     (PTS system lactose-specific EIIB component)

Gene name

Name:

lacE

From

Lactococcus lactis subsp. lactis (Streptococcus lactis)

[TaxID: 1360]

Encoded on

Plasmid pMG820.

Taxonomy

Bacteria; Firmicutes; Lactobacillales; Streptococcaceae; Lactococcus.

Protein existence

4: Predicted;

References

[1]

NUCLEOTIDE SEQUENCE [GENOMIC DNA].
STRAIN=MG1820;
PubMed=2125052 [NCBI, ExPASy, EBI, Israel, Japan]
de Vos W.M., Boerrigter I.J., van Rooyen R.J., Reiche B., Hengstenberg W.;
"Characterization of the lactose-specific enzymes of the phosphotransferase system in Lactococcus lactis.";
J. Biol. Chem. 265:22554-22560(1990).

Comments

FUNCTION: The phosphoenolpyruvate-dependent sugar phosphotransferase system (sugar PTS), a major carbohydrate active -transport system, catalyzes the phosphorylation of incoming sugar substrates concomitantly with their translocation across the cell membrane. This system is involved in lactose transport.
CATALYTIC ACTIVITY: Protein EIIB N(pi)-phospho-L-histidine/cysteine + sugar = protein EIIB + sugar phosphate.
SUBCELLULAR LOCATION: Cell membrane; Multi-pass membrane protein (Probable).
DOMAIN: The EIIC domain forms the PTS system translocation channel and contains the specific substrate-binding site.
DOMAIN: The EIIB domain is phosphorylated by phospho-EIIA on a cysteinyl or histidyl residue, depending on the transported sugar. Then, it transfers the phosphoryl group to the sugar substrate concomitantly with the sugar uptake processed by the EIIC domain.
SIMILARITY: Contains 1 PTS EIIB type-3 domain.
SIMILARITY: Contains 1 PTS EIIC type-3 domain.

Copyright

Copyrighted by the UniProt Consortium, see http://www.uniprot.org/terms. Distributed under the Creative Commons Attribution-NoDerivs License.

Cross-references

Sequence databases

EMBL

M60447; AAA25182.1; -; Genomic_DNA.

[EMBL / GenBank / DDBJ] [CoDingSequence]

PIR

B23696; B23696.

3D structure databases

ModBase

P23531.

Ontologies

GO:0016021;	Cellular component: integral to membrane (inferred from electronic annotation from InterPro).
GO:0005886;	Cellular component: plasma membrane (inferred from electronic annotation from UniProtKB-KW).
GO:0016301;	Molecular function: kinase activity (inferred from electronic annotation from UniProtKB-KW).
GO:0008982;	Molecular function: protein-N(PI)-phosphohistidine-sugar phosphotransferase activity (inferred from electronic annotation from InterPro).
GO:0005351;	Molecular function: sugar:hydrogen symporter activity (inferred from electronic annotation from InterPro).
GO:0009401;	Biological process: phosphoenolpyruvate-dependent sugar phosphotransferase system (inferred from electronic annotation from InterPro).
QuickGo view.

Family and domain databases

InterPro

IPR013012; PTS_EIIB_3.
IPR014350; PTS_EIIB_3_sub.
IPR003352; PTS_EIIC.
IPR003501; PTS_IIB_lac.
IPR004501; PTS_IIC_lac.
IPR004801; PTS_IIC_lac_spec.
Graphical view of domain structure.

Pfam

PF02378; PTS_EIIC; 1.
PF02302; PTS_IIB; 1.
Pfam graphical view of domain structure.

TIGRFAMs

TIGR00394; lac_pts_IIC; 1.
TIGR00410; lacE; 1.
TIGR00853; pts-lac; 1.

PROSITE

PS51100; PTS_EIIB_TYPE_3; 1.
PS51105; PTS_EIIC_TYPE_3; 1.
PROSITE graphical view of domain structure (profiles).

ProtoNet

P23531.

Other

UniRef

View cluster of proteins with at least 50% / 90% / 100% identity.

Keywords

Cell membrane; Kinase; Membrane; Phosphotransferase system; Plasmid; Sugar transport; Transferase; Transmembrane; Transport.

Features

Feature table viewer

Feature aligner

`Key`	`From To`	`Length`	`Description`	`FTId`
`CHAIN`	`1 568`	`568`	`PTS system lactose-specific EIICB component.`	`PRO_0000186584`
`TRANSMEM`	`29 49`	`21`	`Potential.`
`TRANSMEM`	`61 81`	`21`	`Potential.`
`TRANSMEM`	`101 121`	`21`	`Potential.`
`TRANSMEM`	`127 147`	`21`	`Potential.`
`TRANSMEM`	`218 238`	`21`	`Potential.`
`TRANSMEM`	`278 298`	`21`	`Potential.`
`TRANSMEM`	`333 353`	`21`	`Potential.`
`TRANSMEM`	`382 402`	`21`	`Potential.`
`DOMAIN`	`7 409`	`403`	`PTS EIIC type-3.`
`DOMAIN`	`466 568`	`103`	`PTS EIIB type-3.`
`ACT_SITE`	`473 473`		`Phosphocysteine intermediate; for EIIB activity (By similarity).`

Sequence information

Length: 568 AA [This is the length of the unprocessed precursor]

Molecular weight: 61563 Da [This is the MW of the unprocessed precursor]

CRC64: BE5ECA7736762446 [This is a checksum on the sequence]

        10         20         30         40         50         60 
MHKLIELIEK GKPFFEKISR NIYLRAIRDG FIAGMPVILF SSIFILIAYV PNAWGFHWSK 

        70         80         90        100        110        120 
DIETFLMTPY SYSMGILAFF VGGTTAKALT DSKNRDLPAT NQINFLSTML ASMVGFLLMA 

       130        140        150        160        170        180 
AEPAKEGGFL TAFMGTKGLL TAFIAAFVTV NVYKVCVKNN VTIRMPEDVP PNISQVFKDL 

       190        200        210        220        230        240 
IPFTVSVVLL YGLELLVKGT LGVTVAESIG TLIAPLFSAA DGYLGITLIF GAYAFFWFVG 

       250        260        270        280        290        300 
IHGPSIVEPA IAAITYANID VNLHLIQAGQ HADKVITSGT QMFIATMGGT GATLIVPFLF 

       310        320        330        340        350        360 
MWICKSDRNR AIGRASVVPT FFGVNEPILF GAPIVLNPIF FVPFIFAPIV NVWIFKFFVD 

       370        380        390        400        410        420 
TLNMNSFSAN LPWVTPGPLG IVLGTNFQVL SFILAGLLVV VDTIIYYPFV KVYDEQILEE 

       430        440        450        460        470        480 
ERSGKTNDAL KEKVAANFNT AKADAVLGKA DVAKEDVAAN NNITKETNVL VLCAGGGTSG 

       490        500        510        520        530        540 
LLANALNKAA AEYNVPVKAA AGGYGAHREM LPEFDLVILA PQVASNFDDM KAETDKLGIK 

       550        560 
LVKTEGAQYI KLTRDGQGAL AFVQQQFD

P23531 in FASTA format

View entry in original UniProtKB/Swiss-Prot format
View entry in raw text format (no links)
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	BLAST submission on ExPASy/SIB or at NCBI (USA)		Sequence analysis tools: ProtParam, ProtScale, Compute pI/Mw, PeptideMass, PeptideCutter, Dotlet (Java)
	ScanProsite, MotifScan		Submit a homology modeling request to SWISS-MODEL
	NPSA Sequence analysis tools