NUCLEOTIDE SEQUENCE [MRNA].
STRAIN=C57BL/6;
TISSUE=Retina;
DOI=10.1038/347677a0; PubMed=1977087 [NCBI, ExPASy, EBI, Israel, Japan]
Bowes C., Li T., Danciger M., Baxter L.C., Applebury M.L., Farber D.B.;
"Retinal degeneration in the rd mouse is caused by a defect in the beta subunit of rod cGMP-phosphodiesterase.";
Nature 347:677-680(1990).

[2]

NUCLEOTIDE SEQUENCE [MRNA].
TISSUE=Retina;
DOI=10.1016/0014-5793(91)80095-K; PubMed=1847109 [NCBI, ExPASy, EBI, Israel, Japan]
Baehr W., Champagne M.S., Lee A.K., Pittler S.J.;
"Complete cDNA sequences of mouse rod photoreceptor cGMP phosphodiesterase alpha- and beta-subunits, and identification of beta'-, a putative beta-subunit isozyme produced by alternative splicing of the beta-subunit gene.";
FEBS Lett. 278:107-114(1991).

Comments

FUNCTION: This protein participates in processes of transmission and amplification of the visual signal. Necessary for the formation of a functional phosphodiesterase holoenzyme.
CATALYTIC ACTIVITY: Guanosine 3',5'-cyclic phosphate + H₂O = guanosine 5'-phosphate.
SUBUNIT: Oligomer composed of two catalytic chains (alpha and beta), an inhibitory chain (gamma) and the delta chain.
SUBCELLULAR LOCATION: Membrane; Lipid-anchor.
ALTERNATIVE PRODUCTS: 2 named isoforms [FASTA] produced by alternative splicing.

Name 1

Isoform ID P23440-1

This is the isoform sequence displayed in this entry.

Name 2

Synonyms Beta'

Isoform ID P23440-2

Features which should be applied to build the isoform sequence: VSP_004592.
DISEASE: Defects in Pde6b are the cause of retinal degeneration.
SIMILARITY: Belongs to the cyclic nucleotide phosphodiesterase family.
SIMILARITY: Contains 2 GAF domains.

Copyright

Copyrighted by the UniProt Consortium, see http://www.uniprot.org/terms. Distributed under the Creative Commons Attribution-NoDerivs License.

Cross-references

Sequence databases

EMBL

X55968; CAA39439.1; ALT_SEQ; mRNA.	[EMBL / GenBank / DDBJ] [CoDingSequence]
X60133; CAA42719.1; -; mRNA.	[EMBL / GenBank / DDBJ] [CoDingSequence]

PIR

S30762; S30762.

UniGene

Mm.1372

3D structure databases

ModBase

P23440.

PTM databases

PhosphoSite

P23440; -.

Organism-specific databases

MGI

MGI:97525; Pde6b.

Gene expression databases

ArrayExpress

P23440; -.

GermOnline

ENSMUSG00000029491; Mus musculus.

Ontologies

GO:0016020;	Cellular component: membrane (inferred from electronic annotation from UniProtKB-KW).
GO:0047555;	Molecular function: 3',5'-cyclic-GMP phosphodiesterase activity (inferred from electronic annotation from EC).
GO:0009583;	Biological process: detection of light stimulus (inferred from mutant phenotype from MGI).
GO:0007165;	Biological process: signal transduction (inferred from electronic annotation from InterPro).
GO:0007601;	Biological process: visual perception (inferred from electronic annotation from UniProtKB-KW).
QuickGo view.

Family and domain databases

InterPro

IPR003018; GAF.
IPR003607; Met-dep_phosphohydro_HD.
IPR002073; PDEase.
Graphical view of domain structure.

Pfam

PF01590; GAF; 2.
PF00233; PDEase_I; 1.
Pfam graphical view of domain structure.

PRINTS

PR00387; PDIESTERASE1.

SMART

SM00065; GAF; 2.
SM00471; HDc; 1.
SMART graphical view of domain structure.

PROSITE

PS00126; PDEASE_I; 1.

ProtoNet

P23440.

Genome annotation databases

Ensembl

ENSMUSG00000029491; Mus musculus. [Contig view]

Phylogenomic databases

HOGENOM

P23440; -.

HOVERGEN

P23440; -.

Other

SOURCE

Pde6b; Mus musculus.

UniRef

View cluster of proteins with at least 50% / 90% / 100% identity.

Keywords

Alternative splicing; cGMP; Hydrolase; Lipoprotein; Membrane; Prenylation; Repeat; Sensory transduction; Vision.

Features

Feature table viewer

Feature aligner

`Key`	`From To`	`Length`	`Description`	`FTId`
`CHAIN`	`1 853`	`853`	`Rod cGMP-specific 3',5'-cyclic phosphodiesterase subunit beta.`	`PRO_0000023350`
`PROPEP`	`854 856`	`3`	`Removed in mature form (By similarity).`	`PRO_0000023351`
`DOMAIN`	`71 220`	`150`	`GAF 1.`
`DOMAIN`	`252 429`	`178`	`GAF 2.`
`LIPID`	`853 853`		`S-geranylgeranyl cysteine (By similarity).`
`VAR_SEQ`	`801 856`		`Missing (in isoform 2).`	`VSP_004592`
`CONFLICT`	`5 5`		`G -> E (in Ref. 2; CAA42719).`
`CONFLICT`	`19 19`		`S -> A (in Ref. 2; CAA42719).`
`CONFLICT`	`49 50`		`DV -> EL (in Ref. 2; CAA42719).`
`CONFLICT`	`158 158`		`T -> P (in Ref. 2; CAA42719).`
`CONFLICT`	`176 176`		`C -> L (in Ref. 2; CAA42719).`
`CONFLICT`	`232 232`		`R -> E (in Ref. 2; CAA42719).`
`CONFLICT`	`236 236`		`S -> G (in Ref. 2; CAA42719).`

Sequence information

Length: 856 AA [This is the length of the unprocessed precursor]

Molecular weight: 98502 Da [This is the MW of the unprocessed precursor]

CRC64: 3677704D0C7496D1 [This is a checksum on the sequence]

        10         20         30         40         50         60 
MSLSGEQVRS FLDGNPTFSH QYFGKKLSPE NVAGACEDGW LADCGSLRDV CQVEESAALF 

        70         80         90        100        110        120 
ELVQDMQESV NMERVVFKIL RRLCTILHAD RCSLFMYRQR NGIAELATRL FSVQPDSLLE 

       130        140        150        160        170        180 
DCLVPPDSEI VFPLDIGIVG HVAQTKKMIN VQDVAECTHF SSFADELTDY VTKNICSTPI 

       190        200        210        220        230        240 
MNGKDVVAVI MAVNKLDGPC FTSEDEDVFT KYLNFATLNL KIYHLSYLHN CRTRRSQVLL 

       250        260        270        280        290        300 
WSANKVFEEL TDIERQFHKA FYTVRAYLNC ERYSVGLLDM TKEKEFFDVW PVLMGEAQPY 

       310        320        330        340        350        360 
SGPRTPDGRE IVFYKVIDYI LHGKEDIKVI PTPPADHWAL ASGLPTYVAE SGFICNIMNA 

       370        380        390        400        410        420 
SADEMFNFQE GPLDDSGWVI KNVLSMPIVN KKEEIVGVAT FYNRKDGKPF DDQDEVLMES 

       430        440        450        460        470        480 
LTQFLGWSVL NTDTYDKMNK LENRKDIAQD MVLYHVRCDK DEIQEILPTR DRLGKEPADC 

       490        500        510        520        530        540 
EEDELGKILK EELPGPTKFD IYEFHFSDLE CTELELVKCG IQMYYELGVV RKFQIPQEVL 

       550        560        570        580        590        600 
VRFLFSVSKA YRRITYHNWR HGFNVAQTMF TLLMTGKLKS YYTDLEAFAM VTAGLCHDID 

       610        620        630        640        650        660 
HRGTNNLYQM KSQNPLAKLH GSSILERHHL EFGKFLLAEE SLNIYQNLNR RQHEHVIHLM 

       670        680        690        700        710        720 
DIAIIATDLA LYFKKRTMFQ KIVDESKNYE DKKSWVEYLS LETTRKEIVM AMMMTACDLS 

       730        740        750        760        770        780 
AITKPWEVQS KVALLVAAEF WEQGDLERTV LDQQPIPMMD RNKAAELPKL QVGFIDFVCT 

       790        800        810        820        830        840 
FVYKEFSRFH EEILPMFDRL QNNRKEWKAL ADEYEAKVKA LEEEKKKEED RVAAKKVGTE 

       850 
VCNGGPAPKS STCCIL

P23440 in FASTA format

View entry in original UniProtKB/Swiss-Prot format
View entry in raw text format (no links)
Report form for errors/updates in this UniProtKB/Swiss-Prot entry

	BLAST submission on ExPASy/SIB or at NCBI (USA)		Sequence analysis tools: ProtParam, ProtScale, Compute pI/Mw, PeptideMass, PeptideCutter, Dotlet (Java)
	ScanProsite, MotifScan		Submit a homology modeling request to SWISS-MODEL
	NPSA Sequence analysis tools