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UniProtKB/Swiss-Prot entry P23384

[Entry info] [Name and origin] [References] [Comments] [Cross-references] [Keywords] [Features] [Sequence] [Tools]

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Entry information
Entry name NPRE_BACCL
Primary accession number P23384
Secondary accession numbers None
Integrated into Swiss-Prot on November 1, 1991
Sequence was last modified on November 1, 1991 (Sequence version 1)
Annotations were last modified on    November 25, 2008 (Entry version 64)
Name and origin of the protein
Protein name Bacillolysin [Precursor]
Synonyms EC 3.4.24.28
Thermostable neutral protease
Gene name
Name: npr
From
Bacillus caldolyticus [TaxID: 1394] 
Taxonomy Bacteria; Firmicutes; Bacillales; Bacillaceae; Bacillus.
Protein existence 1: Evidence at protein level;
References
[1]
 NUCLEOTIDE SEQUENCE [GENOMIC DNA].
PubMed=1905714 [NCBI, ExPASy, EBI, Israel, Japan]
van den Burg B., Enequist H.G., van de Haar M.E., Eijsink V.G.H., Stulp B.K., Venema G.;
"A highly thermostable neutral protease from Bacillus caldolyticus: cloning and expression of the gene in Bacillus subtilis and characterization of the gene product.";
J. Bacteriol. 173:4107-4115(1991).
Comments
Copyright
Copyrighted by the UniProt Consortium, see http://www.uniprot.org/terms. Distributed under the Creative Commons Attribution-NoDerivs License.
Cross-references
Sequence databases
EMBL
M63575; AAA22623.1; -; Genomic_DNA.[EMBL / GenBank / DDBJ] [CoDingSequence]
PIR A42464; A42464.
3D structure databases
HSSP P00800; 1KJO. [HSSP ENTRY / PDB]
SMR P23384; 226-543.
ModBase P23384.
Ontologies
GO
GO:0005576; Cellular component: extracellular region (inferred from electronic annotation from InterPro).
GO:0005509; Molecular function: calcium ion binding (inferred from electronic annotation from UniProtKB-KW).
GO:0004222; Molecular function: metalloendopeptidase activity (inferred from electronic annotation from InterPro).
GO:0008270; Molecular function: zinc ion binding (inferred from electronic annotation from InterPro).
GO:0006508; Biological process: proteolysis (inferred from electronic annotation from InterPro).
QuickGo view.
Family and domain databases
InterPro IPR005075; Pept_M4_propep_PepSY.
IPR006025; Pept_M_Zn_BS.
IPR013856; Peptidase_M4.
IPR001570; Peptidase_M4_C.
IPR011096; Propep_M4_M36.
Graphical view of domain structure.
Gene3D G3DSA:3.10.170.10; Peptidase_M4; 1.
G3DSA:1.10.390.10; Peptidase_M4/M36; 1.
Pfam PF07504; FTP; 1.
PF03413; PepSY; 1.
PF01447; Peptidase_M4; 1.
PF02868; Peptidase_M4_C; 1.
Pfam graphical view of domain structure.
PRINTS PR00730; THERMOLYSIN.
PROSITE PS00142; ZINC_PROTEASE; 1.
BLOCKS P23384.
ProtoNet P23384.
Other
UniRef View cluster of proteins with at least 50% / 90% / 100% identity.
Keywords
Calcium; Hydrolase; Metal-binding; Metalloprotease; Protease; Secreted; Signal; Zinc; Zymogen.
Features
SEVIEWER logo Feature table viewer FT aligner logo Feature aligner
KeyFrom   To Length Description FTId
SIGNAL   1    25  25      
PROPEP   26   225  200     Activation peptide. PRO_0000028600
CHAIN   226   544  319     Bacillolysin. PRO_0000028601
ACT_SITE   371   371        By similarity. 
ACT_SITE   459   459        Proton donor (By similarity). 
METAL   285   285        Calcium 1 (By similarity). 
METAL   287   287        Calcium 1 (By similarity). 
METAL   289   289        Calcium 1; via carbonyl oxygen (By similarity). 
METAL   366   366        Calcium 2 (By similarity). 
METAL   370   370        Zinc; catalytic (By similarity). 
METAL   374   374        Zinc; catalytic (By similarity). 
METAL   394   394        Zinc; catalytic (By similarity). 
METAL   405   405        Calcium 2 (By similarity). 
METAL   405   405        Calcium 3 (By similarity). 
METAL   411   411        Calcium 3; via carbonyl oxygen (By similarity). 
METAL   413   413        Calcium 2 (By similarity). 
METAL   413   413        Calcium 3 (By similarity). 
METAL   415   415        Calcium 2; via carbonyl oxygen (By similarity). 
METAL   418   418        Calcium 2 (By similarity). 
METAL   418   418        Calcium 3 (By similarity). 
METAL   421   421        Calcium 4; via carbonyl oxygen (By similarity). 
METAL   422   422        Calcium 4 (By similarity). 
METAL   425   425        Calcium 4; via carbonyl oxygen (By similarity). 
METAL   428   428        Calcium 4 (By similarity). 
Sequence information
Length: 544 AA [This is the length of the unprocessed precursor] Molecular weight: 59413 Da [This is the MW of the unprocessed precursor] CRC64: C551E67ACCA356FD [This is a checksum on the sequence] 
        10         20         30         40         50         60 
MNKRAMLGAI GLAFGLMAWP FGASAKGKSM VWNEQWKTPS FVSGSLLGRC SQELVYRYLD 

        70         80         90        100        110        120 
QEKNTFQLGG QARERLSLIG NKLDELGHTV MRFEQAIAAS LCMGAVLVAH VNDGELSSLS 

       130        140        150        160        170        180 
GTLIPNLDKR TLKTEAAISI QQAEMIAKQD VADRVTKERP AAEEGKPTRL VIYPDEETPR 

       190        200        210        220        230        240 
LAYEVNVRFL TPVPGNWIYM IDAADGKVLN KWNQMDEAKP GGAQPVAGTS TVGVGRGVLG 

       250        260        270        280        290        300 
DQKYINTTYS SYYGYYYLQD NTRGSGIFTY DGRNRTVLPG SLWADGDNQF FASYDAAAVD 

       310        320        330        340        350        360 
AHYYAGVVYD YYKNVHGRLS YDGSNAAIRS TVHYGRGYNN AFWNGSQMVY GDGDGQTFLP 

       370        380        390        400        410        420 
FSGGIDVVGH ELTHAVTDYT AGLVYQNESG AINEAMSDIF GTLVEFYANR NPDWEIGEDI 

       430        440        450        460        470        480 
YTPGVAGDAL RSMSDPAKYG DPDHYSKRYT GTQDNGGVHT NSGIINKAAY LLSQGGVHYG 

       490        500        510        520        530        540 
VSVTGIGRDK MGKIFYRALV YYLTPTSNFS QLRAACVQAA ADLYGSTSQE VNSVKQAFNA 


VGVY
P23384 in FASTA format

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