[1]	NUCLEOTIDE SEQUENCE [MRNA]. PubMed=2510162 [NCBI, ExPASy, EBI, Israel, Japan] Larsen R.D., Rajan V.P., Ruff M.M., Kukowska-Latallo J., Cummings R.D., Lowe J.B.; "Isolation of a cDNA encoding a murine UDPgalactose:beta-D-galactosyl-1,4-N-acetyl-D-glucosaminide alpha-1,3-galactosyltransferase: expression cloning by gene transfer."; Proc. Natl. Acad. Sci. U.S.A. 86:8227-8231(1989).
[2]	NUCLEOTIDE SEQUENCE [MRNA], AND ALTERNATIVE SPLICING. PubMed=1544928 [NCBI, ExPASy, EBI, Israel, Japan] Joziasse D.H., Shaper N.L., Kim D., van den Eijnden D.H., Shaper J.H.; "Murine alpha 1,3-galactosyltransferase. A single gene locus specifies four isoforms of the enzyme by alternative splicing."; J. Biol. Chem. 267:5534-5541(1992).
[3]	NUCLEOTIDE SEQUENCE [GENOMIC DNA / MRNA] (ISOFORM 3). STRAIN=BALB/c; DOI=10.1016/S0041-1345(00)02216-8; PubMed=11267031 [NCBI, ExPASy, EBI, Israel, Japan] Koike C., Friday R., Fung J.J., Starzl T.E., Trucco M.; "Comparison of the regulatory regions of the alpha1,3galactosyltransferase gene between murine and porcine species."; Transplant. Proc. 33:710-711(2001).

Comments

FUNCTION: Transfer of galactose from UDP-galactose to an acceptor molecule (R).
CATALYTIC ACTIVITY: UDP-galactose + beta-D-galactosyl-(1->4)-beta-N-acetyl-D-glucosaminyl-R = UDP + alpha-D-galactosyl-(1->3)-beta-D-galactosyl-(1->4)-beta-N-acetylglucosaminyl-R.
COFACTOR: Manganese.
PATHWAY: Protein modification; protein glycosylation .
SUBCELLULAR LOCATION: Golgi apparatus, Golgi stack membrane; Single-pass type II membrane protein. Note=Membrane-bound form in trans cisternae of Golgi.

ALTERNATIVE PRODUCTS: 3 named isoforms [FASTA] produced by alternative splicing.

Name	1
Isoform ID	P23336-1
This is the isoform sequence displayed in this entry.

Name	2
Isoform ID	P23336-2
Features which should be applied to build the isoform sequence: VSP_001804.

Name	3
Isoform ID	P23336-3
Features which should be applied to build the isoform sequence: VSP_001804, VSP_001805.

DOMAIN: The conserved DXD motif is involved in cofactor binding. The manganese ion interacts with the beta-phosphate group of UDP and may also have a role in catalysis.
SIMILARITY: Belongs to the glycosyltransferase 6 family.

Copyright

Copyrighted by the UniProt Consortium, see http://www.uniprot.org/terms. Distributed under the Creative Commons Attribution-NoDerivs License.

Cross-references

Sequence databases

EMBL

M26925; AAA37657.1; -; mRNA.	[EMBL / GenBank / DDBJ] [CoDingSequence]
M85153; AAA37711.1; -; mRNA.	[EMBL / GenBank / DDBJ] [CoDingSequence]
AF297614; AAK97205.1; -; Genomic_DNA.	[EMBL / GenBank / DDBJ] [CoDingSequence]
AF297609; AAK97205.1; JOINED; Genomic_DNA.	[EMBL / GenBank / DDBJ] [CoDingSequence]
AF297610; AAK97205.1; JOINED; Genomic_DNA.	[EMBL / GenBank / DDBJ] [CoDingSequence]
AF297611; AAK97205.1; JOINED; Genomic_DNA.	[EMBL / GenBank / DDBJ] [CoDingSequence]
AF297612; AAK97205.1; JOINED; Genomic_DNA.	[EMBL / GenBank / DDBJ] [CoDingSequence]
AF297613; AAK97205.1; JOINED; Genomic_DNA.	[EMBL / GenBank / DDBJ] [CoDingSequence]
AF297615; AAK97206.1; -; mRNA.	[EMBL / GenBank / DDBJ] [CoDingSequence]

PIR

A34417; A34417.
I49698; I49698.

RefSeq

NP_034413.1; -.

UniGene

Mm.281124

3D structure databases

HSSP

P14769; 1GX4. [HSSP ENTRY / PDB]

SMR

P23336; 110-394.

ModBase

P23336.

Organism-specific databases

MGI

MGI:95704; Ggta1.

Gene expression databases

ArrayExpress

P23336; -.

CleanEx

MM_GGTA1; -.

GermOnline

ENSMUSG00000035778; Mus musculus.

Ontologies

GO:0005794;	Cellular component: Golgi apparatus (inferred from electronic annotation from UniProtKB-KW).
GO:0016021;	Cellular component: integral to membrane (inferred from electronic annotation from UniProtKB-KW).
GO:0030145;	Molecular function: manganese ion binding (inferred from electronic annotation from UniProtKB-KW).
GO:0047276;	Molecular function: N-acetyllactosaminide 3-alpha-galactosyltransferase activity (inferred from electronic annotation from EC).
GO:0005975;	Biological process: carbohydrate metabolic process (inferred from electronic annotation from InterPro).
QuickGo view.

Family and domain databases

InterPro

IPR005076; Glyco_trans_6.
Graphical view of domain structure.

PANTHER

PTHR10462; Glyco_trans_6; 1.

Pfam

PF03414; Glyco_transf_6; 1.
Pfam graphical view of domain structure.

ProtoNet

P23336.

Genome annotation databases

Ensembl

ENSMUSG00000035778; Mus musculus. [Contig view]

GeneID

14594; -.

KEGG

mmu:14594; -.

Phylogenomic databases

HOVERGEN

P23336; -.

Other

NextBio

286352; -.

SOURCE

Ggta1; Mus musculus.

UniRef

View cluster of proteins with at least 50% / 90% / 100% identity.

Keywords

Alternative splicing; Glycoprotein; Glycosyltransferase; Golgi apparatus; Manganese; Membrane; Metal-binding; Signal-anchor; Transferase; Transmembrane.

Features

Feature table viewer

Feature aligner

`Key`	`From To`	`Length`	`Description`	`FTId`
`CHAIN`	`1 394`	`394`	`N-acetyllactosaminide alpha-1,3-galactosyltransferase.`	`PRO_0000157299`
`TOPO_DOM`	`1 41`	`41`	`Cytoplasmic (Potential).`
`TRANSMEM`	`42 60`	`19`	`Signal-anchor for type II membrane protein (Potential).`
`TOPO_DOM`	`61 394`	`334`	`Lumenal (Potential).`
`METAL`	`251 251`		`Manganese (By similarity).`
`METAL`	`253 253`		`Manganese (By similarity).`
`CARBOHYD`	`83 83`		`N-linked (GlcNAc...) (Potential).`
`CARBOHYD`	`319 319`		`N-linked (GlcNAc...) (Potential).`
`VAR_SEQ`	`1 35`		`Missing (in isoform 2 and isoform 3).`	`VSP_001804`
`VAR_SEQ`	`62 62`		`R -> SPDGSFLWIYHTK (in isoform 3).`	`VSP_001805`

Sequence information

Length: 394 AA [This is the length of the unprocessed precursor]

Molecular weight: 46475 Da [This is the MW of the unprocessed precursor]

CRC64: 7766831640D1BBF7 [This is a checksum on the sequence]

        10         20         30         40         50         60 
MITMLQDLHV NKISMSRSKS ETSLPSSRSG SQEKIMNVKG KVILLMLIVS TVVVVFWEYV 

        70         80         90        100        110        120 
NRIPEVGENR WQKDWWFPSW FKNGTHSYQE DNVEGRREKG RNGDRIEEPQ LWDWFNPKNR 

       130        140        150        160        170        180 
PDVLTVTPWK APIVWEGTYD TALLEKYYAT QKLTVGLTVF AVGKYIEHYL EDFLESADMY 

       190        200        210        220        230        240 
FMVGHRVIFY VMIDDTSRMP VVHLNPLHSL QVFEIRSEKR WQDISMMRMK TIGEHILAHI 

       250        260        270        280        290        300 
QHEVDFLFCM DVDQVFQDNF GVETLGQLVA QLQAWWYKAS PEKFTYERRE LSAAYIPFGE 

       310        320        330        340        350        360 
GDFYYHAAIF GGTPTHILNL TRECFKGILQ DKKHDIEAQW HDESHLNKYF LFNKPTKILS 

       370        380        390 
PEYCWDYQIG LPSDIKSVKV AWQTKEYNLV RNNV

P23336 in FASTA format

View entry in original UniProtKB/Swiss-Prot format
View entry in raw text format (no links)
Report form for errors/updates in this UniProtKB/Swiss-Prot entry

	BLAST submission on ExPASy/SIB or at NCBI (USA)		Sequence analysis tools: ProtParam, ProtScale, Compute pI/Mw, PeptideMass, PeptideCutter, Dotlet (Java)
	ScanProsite, MotifScan		Submit a homology modeling request to SWISS-MODEL
	NPSA Sequence analysis tools