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UniProtKB/Swiss-Prot entry P23225

[Entry info] [Name and origin] [References] [Comments] [Cross-references] [Keywords] [Features] [Sequence] [Tools]

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Entry information
Entry name GLTB_MAIZE
Primary accession number P23225
Secondary accession numbers None
Integrated into Swiss-Prot on November 1, 1991
Sequence was last modified on November 1, 1991 (Sequence version 1)
Annotations were last modified on    November 25, 2008 (Entry version 73)
Name and origin of the protein
Protein name Ferredoxin-dependent glutamate synthase, chloroplastic [Precursor]
Synonyms EC 1.4.7.1
Fd-GOGAT
Gene name
Name: GLSF
From
Zea mays (Maize) [TaxID: 4577] 
Taxonomy Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta; Spermatophyta; Magnoliophyta; Liliopsida; Poales; Poaceae; PACCAD clade; Panicoideae; Andropogoneae; Zea.
Protein existence 1: Evidence at protein level;
References
[1]
 NUCLEOTIDE SEQUENCE [MRNA], AND PROTEIN SEQUENCE OF 97-109 AND 790-802.
STRAIN=cv. Golden cross Bantam T51;
TISSUE=Leaf;
PubMed=1989968 [NCBI, ExPASy, EBI, Israel, Japan]
Sakakibara H., Watanabe M., Hase T., Sugiyama T.;
"Molecular cloning and characterization of complementary DNA encoding for ferredoxin-dependent glutamate synthase in maize leaf.";
J. Biol. Chem. 266:2028-2035(1991).
Comments
Copyright
Copyrighted by the UniProt Consortium, see http://www.uniprot.org/terms. Distributed under the Creative Commons Attribution-NoDerivs License.
Cross-references
Sequence databases
EMBL
M59190; AAA33463.1; -; mRNA.[EMBL / GenBank / DDBJ] [CoDingSequence]
PIR A38596; A38596.
RefSeq NP_001105693.1; -.
UniGene Zm.54067
3D structure databases
HSSP P55038; 1OFD. [HSSP ENTRY / PDB]
ModBase P23225.
Organism-specific databases
Gramene P23225; -.
MaizeGDB 40403; -.
Ontologies
GO
GO:0009507; Cellular component: chloroplast (inferred from electronic annotation from UniProtKB-KW).
GO:0051538; Molecular function: 3 iron, 4 sulfur cluster binding (inferred from electronic annotation from UniProtKB-KW).
GO:0016041; Molecular function: glutamate synthase (ferredoxin) activity (inferred from electronic annotation from EC).
GO:0005506; Molecular function: iron ion binding (inferred from electronic annotation from UniProtKB-KW).
GO:0006537; Biological process: glutamate biosynthetic process (inferred from electronic annotation from InterPro).
GO:0006541; Biological process: glutamine metabolic process (inferred from electronic annotation from UniProtKB-KW).
GO:0055114; Biological process: oxidation reduction (inferred from electronic annotation from UniProtKB-KW).
QuickGo view.
Family and domain databases
InterPro IPR013785; Aldolase_TIM.
IPR000583; GATase_2.
IPR002932; Glu_synth_centr_C.
IPR006982; Glu_synth_centr_N.
IPR002489; Glu_synthase_C.
Graphical view of domain structure.
Gene3D G3DSA:3.20.20.70; Aldolase_TIM; 2.
G3DSA:2.160.20.60; Glu_synthase_C; 1.
Pfam PF00310; GATase_2; 1.
PF04898; Glu_syn_central; 1.
PF01645; Glu_synthase; 1.
PF01493; GXGXG; 1.
Pfam graphical view of domain structure.
PROSITE PS51278; GATASE_TYPE_2; 1.
PROSITE graphical view of domain structure (profiles).
ProtoNet P23225.
Genome annotation databases
GeneID 542710; -.
Other
UniRef View cluster of proteins with at least 50% / 90% / 100% identity.
Keywords
3Fe-4S; Amino-acid biosynthesis; Chloroplast; Direct protein sequencing; FAD; Flavoprotein; FMN; Glutamate biosynthesis; Glutamine amidotransferase; Iron; Iron-sulfur; Metal-binding; Oxidoreductase; Plastid; Transit peptide.
Features
SEVIEWER logo Feature table viewer FT aligner logo Feature aligner
KeyFrom    To Length Description FTId
TRANSIT   1     97  97     Chloroplast. 
CHAIN   98   1616  1519     Ferredoxin-dependent glutamate synthase, chloroplastic. PRO_0000011616
DOMAIN   98    497  400     Glutamine amidotransferase type-2. 
NP_BIND   1176   1233  58     FMN (By similarity). 
ACT_SITE   98     98        For GATase activity (By similarity). 
METAL   1229   1229        Iron-sulfur (3Fe-4S) (By similarity). 
METAL   1235   1235        Iron-sulfur (3Fe-4S) (By similarity). 
METAL   1240   1240        Iron-sulfur (3Fe-4S) (By similarity). 
Sequence information
Length: 1616 AA [This is the length of the unprocessed precursor] Molecular weight: 175173 Da [This is the MW of the unprocessed precursor] CRC64: 67724CBFA0AEB053 [This is a checksum on the sequence] 
        10         20         30         40         50         60 
MATLPRAAPP TPAALLPLPR AAPPLLLAGR AAAARRSRLR ARGPSAAARR SWVVASAASS 

        70         80         90        100        110        120 
SSRAVVGGVA RREAPPAPQK PTQQAADLNH ILSERGACGV GFVANLKNMS SFDIVRDALM 

       130        140        150        160        170        180 
ALGCMEHRGG CGADSDSGDG AGLMSAVPWD LFDDWASKQG LALFDRRNTG VGMVFLPQDE 

       190        200        210        220        230        240 
KSMEEAKAAT EKVFVDEGLE VLGWRPVPFN VSVVGRNAKE TMPNIQQIFV KVAKEDNADD 

       250        260        270        280        290        300 
IERELYISRK LIERAAKSFS WADELYFCSL SSRTIVYKGM LRSEVLGQFY LDLQNELYKS 

       310        320        330        340        350        360 
PFAIYHRRFS TNTSPRWPLA QPMRLLGHNG EINTIQGNLN WMRSRETTLK SPVWRGREHE 

       370        380        390        400        410        420 
ICPFGDPKAS DSANLDSTAE LLLRSGRSPA EALMILVPEA YKNHPTLSIK YPEVTDFYDY 

       430        440        450        460        470        480 
YKGQMEAWDG PALLLFSDGR TVGATLDRNG LRPARYWRTS DDFVYVASEV GVIPMDESKV 

       490        500        510        520        530        540 
VMKGRLGPGM MITVDLQTGQ VLENTEVKKT VASASPYGTW LQECTRLIKP VNFLSSTIMD 

       550        560        570        580        590        600 
NETVLRHQQA FGYSSEDVQM VIESMASQGK EPTFCMGDDI PLAVLSQRPH LLYDYFKQRF 

       610        620        630        640        650        660 
AQVTNPAIDP LREGLVMSLE VNIGKRGNIL EVGPENADQV ALSSPVLNEG ELETLLNDSK 

       670        680        690        700        710        720 
LKPKVLSTYF DIRKGLDGSL DKTIQALCEE ADAAVRSGSQ LLVLSDRSEA PEPTRPAIPI 

       730        740        750        760        770        780 
LLAVGAIHQH LIQNGLRMSA SIVADTAQCF STHHFACLIG YGASAVCPYL ALETCRQWRL 

       790        800        810        820        830        840 
SNKTLNLMRN GKMPTVTIEQ AQRNFIKAVK SGLLKILSKM GISLLSSYCG AQIFEIYGLG 

       850        860        870        880        890        900 
QEVVDLAFCG SVSKIGGLTL DELGRETLSF WVKAFSEDTA KRLENFGFIQ SRPGGEYHAN 

       910        920        930        940        950        960 
NPEMSKLLHK AIREKRDNAY TVYQQHLASR PVNVLRDLLE LKSDRAPIPI GKVESATSIV 

       970        980        990       1000       1010       1020 
ERFCTGGMSL GAISRETHEA IAIAMNRIGG KSNSGEGGED PIRWNPLTDV VDGYSPTLPH 

      1030       1040       1050       1060       1070       1080 
LKGLQNGDTA TSAIKQVASG RFGVTPTFLV NADQIEIKIA QGAKPGEGGQ LPGKKVSAYI 

      1090       1100       1110       1120       1130       1140 
ARLRNSKPGV PLISPPPHHD IYSIEDLAQL IYDLHQINPK AKVSVKLVSE AGIGTVASGV 

      1150       1160       1170       1180       1190       1200 
SKANADIIQI SGHDGGTGAS PISSIKHAGG PWELGLTETN QTLIQNGLRE RVVLRVDGGF 

      1210       1220       1230       1240       1250       1260 
RSGQDVLIAA AMGADEYGFG SVAMIATGCV MARICHTNNC PVGVASQREE LRARFPGVPG 

      1270       1280       1290       1300       1310       1320 
DLVNYFLFVA EEVRAALAQL GYEKLDDIIG RTDLLKPKHI SLVKTQHIDL GYLLSNAGLP 

      1330       1340       1350       1360       1370       1380 
EWSSSQIRSQ DVHTNGPVLD ETILADPEIA DAIENEKEVS KAFQIYNVDR AVCGRVAGVI 

      1390       1400       1410       1420       1430       1440 
AKKYGDTGFA GQLNITFNGS AGQSFGCFLT PGMNIRLVGE ANDYVGKGMA GGELVVVPVD 

      1450       1460       1470       1480       1490       1500 
KTGFVPEDAT IVGNTCLYGA TGGQVFVRGK AGERFAVRNS LCQAVVEGTG DHCCEYMTGG 

      1510       1520       1530       1540       1550       1560 
CVVVLGKAGR NVAAGMTGGL AYILDEDDTL VPKVNKEIVK MQRVNAPAGQ MQLKGLIEAY 

      1570       1580       1590       1600       1610 
VEKTGSEKGI AILREWEAYL PLFWQLVPPS EEDSPEACAE FERVLAKQAT TQLSAK
P23225 in FASTA format

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