ExPASy logo ExPASy Home page Site Map Search ExPASy Contact us Swiss-Prot
Notice: This page will be replaced with www.uniprot.org. Please send us your feedback!
Search for

UniProtKB/Swiss-Prot entry P22944

[Entry info] [Name and origin] [References] [Comments] [Cross-references] [Keywords] [Features] [Sequence] [Tools]

Note: most headings are clickable, even if they don't appear as links. They link to the user manual or other documents.
Entry information
Entry name NIR_EMENI
Primary accession number P22944
Secondary accession numbers None
Integrated into Swiss-Prot on August 1, 1991
Sequence was last modified on August 1, 1991 (Sequence version 1)
Annotations were last modified on    November 25, 2008 (Entry version 66)
Name and origin of the protein
Protein name Nitrite reductase [NAD(P)H]
Synonym EC 1.7.1.4
Gene name
Name: niiA
From
Emericella nidulans (Aspergillus nidulans) [TaxID: 162425] 
Taxonomy Eukaryota; Fungi; Dikarya; Ascomycota; Pezizomycotina; Eurotiomycetes; Eurotiomycetidae; Eurotiales; Trichocomaceae; Emericella.
Protein existence 3: Inferred from homology;
References
[1]
 NUCLEOTIDE SEQUENCE [GENOMIC DNA].
DOI=10.1016/0378-1119(90)90178-T; PubMed=2205530 [NCBI, ExPASy, EBI, Israel, Japan]
Johnstone I.L., McCabe P.C., Greaves P., Gurr S.J., Cole G.E., Brow M.A.D., Unkles S.E., Clutterbuck A.J., Kinghorn J.R., Innis M.A.;
"Isolation and characterisation of the crnA-niiA-niaD gene cluster for nitrate assimilation in Aspergillus nidulans.";
Gene 90:181-192(1990).
Comments
Copyright
Copyrighted by the UniProt Consortium, see http://www.uniprot.org/terms. Distributed under the Creative Commons Attribution-NoDerivs License.
Cross-references
Sequence databases
EMBL
M58289; AAA33315.1; -; Genomic_DNA.[EMBL / GenBank / DDBJ] [CoDingSequence]
PIR JH0181; JH0181.
3D structure databases
HSSP P37332; 1FQT. [HSSP ENTRY / PDB]
ModBase P22944.
Ontologies
GO
GO:0051537; Molecular function: 2 iron, 2 sulfur cluster binding (inferred from electronic annotation from InterPro).
GO:0051539; Molecular function: 4 iron, 4 sulfur cluster binding (inferred from electronic annotation from UniProtKB-KW).
GO:0009055; Molecular function: electron carrier activity (inferred from electronic annotation from InterPro).
GO:0050660; Molecular function: FAD binding (inferred from electronic annotation from InterPro).
GO:0020037; Molecular function: heme binding (inferred from electronic annotation from InterPro).
GO:0005506; Molecular function: iron ion binding (inferred from electronic annotation from UniProtKB-KW).
GO:0050661; Molecular function: NADP binding (inferred from electronic annotation from InterPro).
GO:0008942; Molecular function: nitrite reductase [NAD(P)H] activity (inferred from electronic annotation from InterPro).
GO:0042128; Biological process: nitrate assimilation (inferred from electronic annotation from InterPro).
GO:0055114; Biological process: oxidation reduction (inferred from electronic annotation from InterPro).
QuickGo view.
Family and domain databases
InterPro IPR007419; BFD_Fer2_bd.
IPR013027; FAD_pyr_nucl-diS_OxRdtase.
IPR006066; Nir_Si_BS.
IPR006067; Nir_Sir_4Fe4S.
IPR005117; NiRdtase/SiRdtase_haem-b_fer.
IPR012744; Nitri_red_NirB.
IPR012748; Nitri_red_NirD.
IPR001327; Pyr_OxRdtase_NAD_bd.
IPR005806; Rieske_reg.
Graphical view of domain structure.
Gene3D G3DSA:2.102.10.10; Rieske_reg; 1.
Pfam PF04324; Fer2_BFD; 1.
PF01077; NIR_SIR; 1.
PF03460; NIR_SIR_ferr; 1.
PF00070; Pyr_redox; 1.
PF07992; Pyr_redox_2; 1.
PF00355; Rieske; 1.
Pfam graphical view of domain structure.
PRINTS PR00368; FADPNR.
PR00397; SIROHAEM.
ProDom PD000139; FAD_pyr_redox; 1.
[Domain structure / List of seq. sharing at least 1 domain]
TIGRFAMs TIGR02378; nirD_assim_sml; 1.
TIGR02374; nitri_red_nirB; 1.
PROSITE PS00365; NIR_SIR; 1.
PS51296; RIESKE; 1.
PROSITE graphical view of domain structure (profiles).
ProtoNet P22944.
Other
UniRef View cluster of proteins with at least 50% / 90% / 100% identity.
Keywords
2Fe-2S; 4Fe-4S; FAD; Flavoprotein; Heme; Iron; Iron-sulfur; Metal-binding; NADP; Nitrate assimilation; Oxidoreductase.
Features
SEVIEWER logo Feature table viewer FT aligner logo Feature aligner
KeyFrom    To Length Description FTId
CHAIN   1   1104  1104     Nitrite reductase [NAD(P)H]. PRO_0000199958
DOMAIN   932   1040  109     Rieske. 
NP_BIND   44     79  36     FAD (Potential). 
NP_BIND   146    176  31     NAD or NADP (Potential). 
METAL   720    720        Iron-sulfur (4Fe-4S) (By similarity). 
METAL   726    726        Iron-sulfur (4Fe-4S) (By similarity). 
METAL   760    760        Iron-sulfur (4Fe-4S) (By similarity). 
METAL   764    764        Iron (siroheme axial ligand) (By similarity). 
METAL   764    764        Iron-sulfur (4Fe-4S) (By similarity). 
METAL   976    976        Iron-sulfur (2Fe-2S) (By similarity). 
METAL   978    978        Iron-sulfur (2Fe-2S); via pros nitrogen (By similarity). 
METAL   1001   1001        Iron-sulfur (2Fe-2S) (By similarity). 
METAL   1004   1004        Iron-sulfur (2Fe-2S); via pros nitrogen (By similarity). 
Sequence information
Length: 1104 AA [This is the length of the unprocessed precursor] Molecular weight: 122732 Da [This is the MW of the unprocessed precursor] CRC64: E5D00436E1E86304 [This is a checksum on the sequence] 
        10         20         30         40         50         60 
MPLLDGPRNG ETVTASAHNG IPIIDGVDPS TLRGDIDQDP NRRQKIVVVG LGMVAVAFIE 

        70         80         90        100        110        120 
KLVKLDSERR KYDIVVIGEE PHIAYNRVGL SSYFEHRKIE DLYLNPKEWY GSFKDRSFDY 

       130        140        150        160        170        180 
YLNTRVTDVF PQHKTVKTST GDIVSYDILV LATGSDAVLP TSTPGHDAKG IFVYRTISDL 

       190        200        210        220        230        240 
ERLMEFAANH KGQTGVTVGG GLLGLEAAKA MTDLEDFGSV KLIDRNKWVL ARQLDGDAGS 

       250        260        270        280        290        300 
LVTKKIRDLG LEVLHEKRVA KIHTDDDNNV TGILFEDGQE LDCCCICFAI GIRPRDELGG 

       310        320        330        340        350        360 
STGIQCAKRG GFVIDESLRT SVNDIYAIGE CASWENQTFG IIAPGIEMAD VLSFNLTNPD 

       370        380        390        400        410        420 
KEPKRFNRPD LSTKLKLLGV DVASFGDFFA DRDGPKFLPG QRPSAESIGA ADPNREEEPQ 

       430        440        450        460        470        480 
VKALTYRDPF GGVYKKYLFT MDGKYLLGGM MIGDTKDYVK LNQMVKSQKP LEVPPSEFIL 

       490        500        510        520        530        540 
GAQSGGEENA DDLDDSTQIC SCHNVTKGDV VESVKSGTCK TIADVKSCTK AGTGCGGCMP 

       550        560        570        580        590        600 
LVQSIFNKTM LDMGQEVSNN LCVHIPYSRA DLYNVIAIRQ LRTFDDVMKS AGKCPDSLGC 

       610        620        630        640        650        660 
EICKPAIASI LSSLFNPHLM DKEYHELQET NDRFLANIQR NGTFSVVPRV PGGEITADKL 

       670        680        690        700        710        720 
IAIGQVAKKY NLYCKITGGQ RIDMFGARKQ DLLDIWTELV DAGMESGHRY AKSLRTVKSC 

       730        740        750        760        770        780 
VGTTWCRFGV GDSVGMAIRL EQRYKSIRAP HKFKGAVSGC VRECAEAQNK DFGLIATEKG 

       790        800        810        820        830        840 
FNIFVGGNGG AKPRHSELLA KDVPPEEVIP ILDRYVIFYI RTADKLQRTA RWLESLPGGI 

       850        860        870        880        890        900 
EYLKDVVLND KLGIAAEMER QMQELVDSYF CEWTETVRNP KRRKYFQQFA NTDETVENVE 

       910        920        930        940        950        960 
IVKEREQVRP TYWPKDGANE DFKGHQWSSL SWQPVIKADY FSDGPPAISS ANIKRGDTQL 

       970        980        990       1000       1010       1020 
AIFKVKGKYY ATQQMCPHKR TFVLSDGLIG DDDNGKYWVS CPYHKRNFEL NGEQAGRCQN 

      1030       1040       1050       1060       1070       1080 
DEAMNIATFP VEEREDGWIY MKLPPVEELD SVLGTEKWKV KKGEAVDPFE AYDKKYSGMK 

      1090       1100 
GKRAGAKGIE GSKPTRSPSN TIDW
P22944 in FASTA format

View entry in original UniProtKB/Swiss-Prot format
View entry in raw text format (no links)
Report form for errors/updates in this UniProtKB/Swiss-Prot entry

BLAST logo BLAST submission on ExPASy/SIB
or at NCBI (USA) 		Tools Sequence analysis tools: ProtParam, ProtScale, Compute pI/Mw, PeptideMass, PeptideCutter, Dotlet (Java)
PROSITE logo ScanProsite, MotifScan SWISS-MODEL Submit a homology modeling request to SWISS-MODEL
NPSA logo NPSA Sequence analysis tools

ExPASy logo ExPASy Home page Site Map Search ExPASy Contact us Swiss-Prot
Hosted by ca flag CBR Canada Mirror sites: Australia Brazil China Korea Switzerland
Notice: This page will be replaced with www.uniprot.org. Please send us your feedback!