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UniProtKB/Swiss-Prot entry P22848

[Entry info] [Name and origin] [References] [Comments] [Cross-references] [Keywords] [Features] [Sequence] [Tools]

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Entry information
Entry name 5NTD_VIBPA
Primary accession number P22848
Secondary accession numbers None
Integrated into Swiss-Prot on August 1, 1991
Sequence was last modified on April 4, 2003 (Sequence version 2)
Annotations were last modified on    November 25, 2008 (Entry version 76)
Name and origin of the protein
Protein name 5'-nucleotidase [Precursor]
Synonym EC 3.1.3.5
Gene name
Name: nutA
OrderedLocusNames: VP0748
From
Vibrio parahaemolyticus [TaxID: 670] [HAMAP proteome]
Taxonomy Bacteria; Proteobacteria; Gammaproteobacteria; Vibrionales; Vibrionaceae; Vibrio.
Protein existence 3: Inferred from homology;
References
[1]
 NUCLEOTIDE SEQUENCE [GENOMIC DNA].
STRAIN=AQ3334;
PubMed=2016269 [NCBI, ExPASy, EBI, Israel, Japan]
Tamao Y., Noguchi K., Sakai-Tomita Y., Hama H., Shimamoto T., Kanazawa H., Tsuda M., Tsuchiya T.;
"Sequence analysis of nutA gene encoding membrane-bound Cl(-)-dependent 5'-nucleotidase of Vibrio parahaemolyticus.";
J. Biochem. 109:24-29(1991).
[2]
 NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
STRAIN=RIMD 2210633 / Serotype O3:K6;
DOI=10.1016/S0140-6736(03)12659-1; PubMed=12620739 [NCBI, ExPASy, EBI, Israel, Japan]
Makino K., Oshima K., Kurokawa K., Yokoyama K., Uda T., Tagomori K., Iijima Y., Najima M., Nakano M., Yamashita A., Kubota Y., Kimura S., Yasunaga T., Honda T., Shinagawa H., Hattori M., Iida T.;
"Genome sequence of Vibrio parahaemolyticus: a pathogenic mechanism distinct from that of V. cholerae.";
Lancet 361:743-749(2003).
Comments
Copyright
Copyrighted by the UniProt Consortium, see http://www.uniprot.org/terms. Distributed under the Creative Commons Attribution-NoDerivs License.
Cross-references
Sequence databases
EMBL
X57711; CAA40882.1; -; Genomic_DNA.[EMBL / GenBank / DDBJ] [CoDingSequence]
D00910; BAA00756.1; -; Genomic_DNA.[EMBL / GenBank / DDBJ] [CoDingSequence]
BA000031; BAC59011.1; -; Genomic_DNA.[EMBL / GenBank / DDBJ] [CoDingSequence]
PIR JX0153; JX0153.
RefSeq NP_797127.1; -.
3D structure databases
HSSP P07024; 2USH. [HSSP ENTRY / PDB]
ModBase P22848.
Enzyme and pathway databases
BioCyc VPAR223926:VP0748-MON; -.
Ontologies
GO
GO:0009279; Cellular component: cell outer membrane (inferred from electronic annotation from UniProtKB-KW).
GO:0005886; Cellular component: plasma membrane (inferred from electronic annotation from UniProtKB-KW).
GO:0008253; Molecular function: 5'-nucleotidase activity (inferred from electronic annotation from EC).
GO:0000287; Molecular function: magnesium ion binding (inferred from electronic annotation from UniProtKB-KW).
GO:0000166; Molecular function: nucleotide binding (inferred from electronic annotation from InterPro).
GO:0009166; Biological process: nucleotide catabolic process (inferred from electronic annotation from InterPro).
QuickGo view.
Family and domain databases
InterPro IPR008334; 5'-Nucleotdase_C.
IPR006146; 5'-Nucleotdase_CS.
IPR006179; 5_nucleotidase.
IPR004843; M-pesterase.
Graphical view of domain structure.
Gene3D G3DSA:3.90.780.10; 5'-Nucleotdase_C; 1.
PANTHER PTHR11575; 5_nucleotidase; 1.
Pfam PF02872; 5_nucleotid_C; 1.
PF00149; Metallophos; 1.
Pfam graphical view of domain structure.
PRINTS PR01607; APYRASEFAMLY.
PROSITE PS00785; 5_NUCLEOTIDASE_1; 1.
PS00786; 5_NUCLEOTIDASE_2; 1.
PS51257; PROKAR_LIPOPROTEIN; 1.
PROSITE graphical view of domain structure (profiles).
ProtoNet P22848.
Genome annotation databases
GeneID 1188243; -.
GenomeReviews BA000031_GR; VP0748.
KEGG vpa:VP0748; -.
NMPDR fig|223926.1.peg.748; -.
Phylogenomic databases
HOGENOM P22848; -.
Genome annotation databases
CMR P22848; VP0748.
Other
UniRef View cluster of proteins with at least 50% / 90% / 100% identity.
Keywords
Cell membrane; Cell outer membrane; Complete proteome; Hydrolase; Lipoprotein; Magnesium; Membrane; Metal-binding; Nucleotide-binding; Palmitate; Signal.
Features
SEVIEWER logo Feature table viewer FT aligner logo Feature aligner
KeyFrom   To Length Description FTId
SIGNAL   1    21  21     Potential. 
CHAIN   22   560  539     5'-nucleotidase. PRO_0000000029
REGION   501   507  7     Substrate binding (By similarity). 
METAL   45    45        Divalent metal cation 1 (By similarity). 
METAL   47    47        Divalent metal cation 1 (By similarity). 
METAL   88    88        Divalent metal cation 1 (By similarity). 
METAL   88    88        Divalent metal cation 2 (By similarity). 
METAL   120   120        Divalent metal cation 2 (By similarity). 
METAL   221   221        Divalent metal cation 2 (By similarity). 
METAL   256   256        Divalent metal cation 2 (By similarity). 
METAL   258   258        Divalent metal cation 1 (By similarity). 
BINDING   432   432        Substrate (By similarity). 
SITE   121   121  1     Transition state stabilizer (By similarity). 
SITE   124   124  1     Transition state stabilizer (By similarity). 
LIPID   22    22        N-palmitoyl cysteine (Probable). 
LIPID   22    22        S-diacylglycerol cysteine (Probable). 
CONFLICT   198   198        V -> A (in Ref. 1; CAA40882/BAA00756). 
Sequence information
Length: 560 AA [This is the length of the unprocessed precursor] Molecular weight: 62175 Da [This is the MW of the unprocessed precursor] CRC64: F82E6B4095403D05 [This is a checksum on the sequence] 
        10         20         30         40         50         60 
MNQRLIIKTA LSAAILASLA GCASQPAHEW NADTTYKLTV LHTNDHHGRF WQNKHGEYGM 

        70         80         90        100        110        120 
AARKTLIDDL RDEIQAEGGS VLLLSGGDIN TGVPESDLQD AEPDFKGMSK IGYDAMALGN 

       130        140        150        160        170        180 
HEFDNPLDVL FKQQDWANFP MLSANIYDKK TGKRLFQPYA MFNKQGIKIA VIGLTTEDTA 

       190        200        210        220        230        240 
KLGNPEFIGQ VDFRDPKVEA KELIAELKKT ENPDLIFAVT HMGHYENGNR GINAPGDVAL 

       250        260        270        280        290        300 
ARYLNEGDLD MIVGGHSQEP VCMEGPNVIK KNFKPGDECQ PDQQNGTYIV QAHEWGKYVG 

       310        320        330        340        350        360 
RADYEFRNGE LSMVSYDLIP VNLKKKINVD GQSQRVFVQD EITQDKAMLD FLRPFQEKGQ 

       370        380        390        400        410        420 
SQLNVKIAES NGKLEGDRDV VRFQQTNLGR LIATAHMERA KADFAVMNSG GVRDSIEAGD 

       430        440        450        460        470        480 
ITYKDVLTVQ PFGNMVSYVD MSGQEVLDYL NIVATKPVDS GAYAQFAGIS MRIENDKVTN 

       490        500        510        520        530        540 
VFIGNKQLRL DGRYRFTVPS YNASGGDGYP KIDTHPGYVN TGFTDAEVLK DYLESHSPID 

       550        560 
VNEYAPSGEV MYQTNNVVNQ
P22848 in FASTA format

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