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UniProtKB/Swiss-Prot entry P22817


[Entry info] [Name and origin] [References] [Comments] [Cross-references] [Keywords] [Features] [Sequence] [Tools]

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Entry information
Entry name IDE_DROME
Primary accession number P22817
Secondary accession numbers Q86MR2 Q95S06 Q9VPG8
Integrated into Swiss-Prot on August 1, 1991
Sequence was last modified on January 23, 2007 (Sequence version 4)
Annotations were last modified on    November 25, 2008 (Entry version 68)
Name and origin of the protein
Protein name Insulin-degrading enzyme
Synonyms EC 3.4.24.56
Insulin protease
Insulinase
Insulysin
Gene name
Name: Ide
ORFNames: CG5517
From
Drosophila melanogaster (Fruit fly) [TaxID: 7227] 
Taxonomy Eukaryota; Metazoa; Arthropoda; Hexapoda; Insecta; Pterygota; Neoptera; Endopterygota; Diptera; Brachycera; Muscomorpha; Ephydroidea; Drosophilidae; Drosophila; Sophophora.
Protein existence 1: Evidence at protein level;
References
[1]
NUCLEOTIDE SEQUENCE [MRNA], AND PARTIAL PROTEIN SEQUENCE.
PubMed=2126597 [NCBI, ExPASy, EBI, Israel, Japan]
Kuo W.L., Gehm B.D., Rosner M.R.;
"Cloning and expression of the cDNA for a Drosophila insulin-degrading enzyme.";
Mol. Endocrinol. 4:1580-1591(1990).
[2]
NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
STRAIN=Berkeley;
DOI=10.1126/science.287.5461.2185; PubMed=10731132 [NCBI, ExPASy, EBI, Israel, Japan]
Adams M.D., Celniker S.E., Holt R.A., Evans C.A., Gocayne J.D., Amanatides P.G., Scherer S.E., Li P.W., Hoskins R.A., Galle R.F., George R.A., Lewis S.E., Richards S., Ashburner M., Henderson S.N., Sutton G.G., Wortman J.R., Yandell M.D., Zhang Q., Chen L.X., Brandon R.C., Rogers Y.-H.C., Blazej R.G., Champe M., Pfeiffer B.D., Wan K.H., Doyle C., Baxter E.G., Helt G., Nelson C.R., Miklos G.L.G., Abril J.F., Agbayani A., An H.-J., Andrews-Pfannkoch C., Baldwin D., Ballew R.M., Basu A., Baxendale J., Bayraktaroglu L., Beasley E.M., Beeson K.Y., Benos P.V., Berman B.P., Bhandari D., Bolshakov S., Borkova D., Botchan M.R., Bouck J., Brokstein P., Brottier P., Burtis K.C., Busam D.A., Butler H., Cadieu E., Center A., Chandra I., Cherry J.M., Cawley S., Dahlke C., Davenport L.B., Davies P., de Pablos B., Delcher A., Deng Z., Mays A.D., Dew I., Dietz S.M., Dodson K., Doup L.E., Downes M., Dugan-Rocha S., Dunkov B.C., Dunn P., Durbin K.J., Evangelista C.C., Ferraz C., Ferriera S., Fleischmann W., Fosler C., Gabrielian A.E., Garg N.S., Gelbart W.M., Glasser K., Glodek A., Gong F., Gorrell J.H., Gu Z., Guan P., Harris M., Harris N.L., Harvey D.A., Heiman T.J., Hernandez J.R., Houck J., Hostin D., Houston K.A., Howland T.J., Wei M.-H., Ibegwam C., Jalali M., Kalush F., Karpen G.H., Ke Z., Kennison J.A., Ketchum K.A., Kimmel B.E., Kodira C.D., Kraft C.L., Kravitz S., Kulp D., Lai Z., Lasko P., Lei Y., Levitsky A.A., Li J.H., Li Z., Liang Y., Lin X., Liu X., Mattei B., McIntosh T.C., McLeod M.P., McPherson D., Merkulov G., Milshina N.V., Mobarry C., Morris J., Moshrefi A., Mount S.M., Moy M., Murphy B., Murphy L., Muzny D.M., Nelson D.L., Nelson D.R., Nelson K.A., Nixon K., Nusskern D.R., Pacleb J.M., Palazzolo M., Pittman G.S., Pan S., Pollard J., Puri V., Reese M.G., Reinert K., Remington K., Saunders R.D.C., Scheeler F., Shen H., Shue B.C., Siden-Kiamos I., Simpson M., Skupski M.P., Smith T.J., Spier E., Spradling A.C., Stapleton M., Strong R., Sun E., Svirskas R., Tector C., Turner R., Venter E., Wang A.H., Wang X., Wang Z.-Y., Wassarman D.A., Weinstock G.M., Weissenbach J., Williams S.M., Woodage T., Worley K.C., Wu D., Yang S., Yao Q.A., Ye J., Yeh R.-F., Zaveri J.S., Zhan M., Zhang G., Zhao Q., Zheng L., Zheng X.H., Zhong F.N., Zhong W., Zhou X., Zhu S.C., Zhu X., Smith H.O., Gibbs R.A., Myers E.W., Rubin G.M., Venter J.C.;
"The genome sequence of Drosophila melanogaster.";
Science 287:2185-2195(2000).
[3]
GENOME REANNOTATION.
PubMed=12537572 [NCBI, ExPASy, EBI, Israel, Japan]
Misra S., Crosby M.A., Mungall C.J., Matthews B.B., Campbell K.S., Hradecky P., Huang Y., Kaminker J.S., Millburn G.H., Prochnik S.E., Smith C.D., Tupy J.L., Whitfield E.J., Bayraktaroglu L., Berman B.P., Bettencourt B.R., Celniker S.E., de Grey A.D.N.J., Drysdale R.A., Harris N.L., Richter J., Russo S., Schroeder A.J., Shu S.Q., Stapleton M., Yamada C., Ashburner M., Gelbart W.M., Rubin G.M., Lewis S.E.;
"Annotation of the Drosophila melanogaster euchromatic genome: a systematic review.";
Genome Biol. 3:RESEARCH0083.1-RESEARCH0083.22(2002).
[4]
NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
STRAIN=Berkeley;
TISSUE=Embryo;
Stapleton M., Brokstein P., Hong L., Agbayani A., Carlson J.W., Champe M., Chavez C., Dorsett V., Dresnek D., Farfan D., Frise E., George R.A., Gonzalez M., Guarin H., Kronmiller B., Li P.W., Liao G., Miranda A., Mungall C.J., Nunoo J., Pacleb J.M., Paragas V., Park S., Patel S., Phouanenavong S., Wan K.H., Yu C., Lewis S.E., Rubin G.M., Celniker S.E.;
Submitted (MAR-2003) to the EMBL/GenBank/DDBJ databases.
[5]
NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 663-990.
STRAIN=Berkeley;
TISSUE=Embryo;
PubMed=12537569 [NCBI, ExPASy, EBI, Israel, Japan]
Stapleton M., Carlson J.W., Brokstein P., Yu C., Champe M., George R.A., Guarin H., Kronmiller B., Pacleb J.M., Park S., Wan K.H., Rubin G.M., Celniker S.E.;
"A Drosophila full-length cDNA resource.";
Genome Biol. 3:RESEARCH0080.1-RESEARCH0080.8(2002).
[6]
PROTEIN SEQUENCE OF 2-16, FUNCTION, INDUCTION, AND BIOPHYSICOCHEMICAL PROPERTIES.
DOI=10.1021/bi00412a006; PubMed=3139025 [NCBI, ExPASy, EBI, Israel, Japan]
Garcia J.V., Fenton B.W., Rosner M.R.;
"Isolation and characterization of an insulin-degrading enzyme from Drosophila melanogaster.";
Biochemistry 27:4237-4244(1988).
Comments
Copyright
Copyrighted by the UniProt Consortium, see http://www.uniprot.org/terms. Distributed under the Creative Commons Attribution-NoDerivs License.
Cross-references
Sequence databases
EMBL
M58465; AAA28439.1; -; mRNA.[EMBL / GenBank / DDBJ] [CoDingSequence]
AE014296; AAF51584.2; ALT_INIT; Genomic_DNA.[EMBL / GenBank / DDBJ] [CoDingSequence]
BT006006; AAO74689.1; ALT_INIT; mRNA.[EMBL / GenBank / DDBJ] [CoDingSequence]
AY061015; AAL28563.1; ALT_INIT; mRNA.[EMBL / GenBank / DDBJ] [CoDingSequence]
PIR A37254; SNFFIN.
RefSeq NP_524182.2; -.
UniGene Dm.7706
3D structure databases
ModBase P22817.
Protein family/group databases
MEROPS M16.002; -.
M16.984; -.
Organism-specific databases
FlyBase FBgn0001247; Ide.
Gene expression databases
ArrayExpress P22817; -.
GermOnline CG5517; Drosophila melanogaster.
Ontologies
GO
GO:0004222; Molecular function: metalloendopeptidase activity (inferred from electronic annotation from InterPro).
GO:0008270; Molecular function: zinc ion binding (inferred from electronic annotation from InterPro).
GO:0006508; Biological process: proteolysis (inferred from electronic annotation from InterPro).
QuickGo view.
Family and domain databases
InterPro IPR011237; Pept_M16_core.
IPR011765; Pept_M16_N.
IPR001431; Pept_M16_Zn_BS.
IPR007863; Peptidase_M16_C.
Graphical view of domain structure.
Gene3D G3DSA:3.30.830.10; Pept_M16_core; 1.
Pfam PF00675; Peptidase_M16; 1.
PF05193; Peptidase_M16_C; 2.
Pfam graphical view of domain structure.
PROSITE PS00143; INSULINASE; 1.
ProtoNet P22817.
Genome annotation databases
Ensembl CG5517; Drosophila melanogaster. [Contig view]
GeneID 40248; -.
KEGG dme:Dmel_CG5517; -.
NMPDR fig|7227.3.peg.10731; -.
Phylogenomic databases
HOGENOM P22817; -.
Other
NextBio 817784; -.
UniRef View cluster of proteins with at least 50% / 90% / 100% identity.
Keywords
Complete proteome; Direct protein sequencing; Hydrolase; Metal-binding; Metalloprotease; Protease; Zinc.
Features
SEVIEWER logo Feature table viewer
KeyFrom   To Length Description FTId
INIT_MET   1     1        Removed. 
CHAIN   2   990  989     Insulin-degrading enzyme. PRO_0000074407
ACT_SITE   84    84        Proton acceptor (By similarity). 
METAL   81    81        Zinc (By similarity). 
METAL   85    85        Zinc (By similarity). 
METAL   162   162        Zinc (By similarity). 
CONFLICT   11    11        A -> V (in Ref. 1; AAA28439). 
CONFLICT   384   384        F -> L (in Ref. 1; AAA28439). 
CONFLICT   402   403        QP -> ES (in Ref. 1; AAA28439). 
CONFLICT   415   415        Q -> K (in Ref. 1; AAA28439). 
CONFLICT   433   433        R -> S (in Ref. 1; AAA28439). 
Sequence information
Length: 990 AA [This is the length of the unprocessed precursor] Molecular weight: 113684 Da [This is the MW of the unprocessed precursor] CRC64: 32D19736CB497DCB [This is a checksum on the sequence]
        10         20         30         40         50         60 
MTIAESSQKS ATRKPDSMEP ILRLNNIEKS LQDTRDYRGL QLENGLKVLL ISDPNTDVSA 

        70         80         90        100        110        120 
AALSVQVGHM SDPTNLPGLA HFCEHMLFLG TEKYPHENGY TTYLSQSGGS SNAATYPLMT 

       130        140        150        160        170        180 
KYHFHVAPDK LDGALDRFAQ FFIAPLFTPS ATEREINAVN SEHEKNLPSD LWRIKQVNRH 

       190        200        210        220        230        240 
LAKPDHAYSK FGSGNKTTLS EIPKSKNIDV RDELLKFHKQ WYSANIMCLA VIGKESLDEL 

       250        260        270        280        290        300 
EGMVLEKFSE IENKNVKVPG WPRHPYAEER YGQKVKIVPI KDIRSLTISF TTDDLTQFYK 

       310        320        330        340        350        360 
SGPDNYLTHL IGHEGKGSIL SELRRLGWCN DLMAGHQNTQ NGFGFFDIVV DLTQEGLEHV 

       370        380        390        400        410        420 
DDIVKIVFQY LEMLRKEGPK KWIFDECVKL NEMRFRFKEK EQPENLVTHA VSSMQIFPLE 

       430        440        450        460        470        480 
EVLIAPYLSN EWRPDLIKGL LDELVPSKSR IVIVSQSFEP DCDLAEPYYK TKYGITRVAK 

       490        500        510        520        530        540 
DTVQSWENCE LNENLKLALP NSFIPTNFDI SDVPADAPKH PTIILDTPIL RVWHKQDNQF 

       550        560        570        580        590        600 
NKPKACMTFD MSNPIAYLDP LNCNLNHMMV MLLKDQLNEY LYDAELASLK LSVMGKSCGI 

       610        620        630        640        650        660 
DFTIRGFSDK QVVLLEKLLD HLFDFSIDEK RFDILKEEYV RSLKNFKAEQ PYQHSIYYLA 

       670        680        690        700        710        720 
LLLTENAWAN MELLDAMELV TYDRVLNFAK EFFQRLHTEC FIFGNVTKQQ ATDIAGRVNT 

       730        740        750        760        770        780 
RLEATNASKL PILARQMLKK REYKLLAGDS YLFEKENEFH KSSCAQLYLQ CGAQTDHTNI 

       790        800        810        820        830        840 
MVNLVSQVLS EPCYDCLRTK EQLGYIVFSG VRKVNGANGI RIIVQSAKHP SYVEDRIENF 

       850        860        870        880        890        900 
LQTYLQVIED MPLDEFERHK EALAVKKLEK PKTIFQQFSQ FYGEIAMQTY HFEREEAEVA 

       910        920        930        940        950        960 
ILRKISKADF VDYFKKFIAK DGEERRVLSV HIVSQQTDEN ATSEAEPVEI TNMERHKPIS 

       970        980        990 
DIVTFKSCKE LYPIALPFLD IKAKGARSKL 

P22817 in FASTA format

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