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UniProtKB/Swiss-Prot entry P22636

[Entry info] [Name and origin] [References] [Comments] [Cross-references] [Keywords] [Features] [Sequence] [Tools]

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Entry information
Entry name PCYB_PSEPA
Primary accession number P22636
Secondary accession numbers None
Integrated into Swiss-Prot on August 1, 1991
Sequence was last modified on August 1, 1991 (Sequence version 1)
Annotations were last modified on    November 25, 2008 (Entry version 45)
Name and origin of the protein
Protein name Protocatechuate 4,5-dioxygenase beta chain
Synonyms EC 1.13.11.8
4,5-PCD
Gene name
Name: ligB
From
Pseudomonas paucimobilis (Sphingomonas paucimobilis) [TaxID: 13689] 
Taxonomy Bacteria; Proteobacteria; Alphaproteobacteria; Sphingomonadales; Sphingomonadaceae; Sphingomonas.
Protein existence 1: Evidence at protein level;
References
[1]
 NUCLEOTIDE SEQUENCE [GENOMIC DNA], AND PROTEIN SEQUENCE OF 1-9.
STRAIN=SYK-6;
PubMed=2185230 [NCBI, ExPASy, EBI, Israel, Japan]
Noda Y., Nishikawa S., Shiozuka K., Kadokura H., Nakajima H., Yoda K., Katayama Y., Morohoshi N., Haraguchi T., Yamasaki M.;
"Molecular cloning of the protocatechuate 4,5-dioxygenase genes of Pseudomonas paucimobilis.";
J. Bacteriol. 172:2704-2709(1990).
Comments
  • FUNCTION: Responsible for the aromatic ring fission of protocatechuate.
  • CATALYTIC ACTIVITY: Protocatechuate + O2 = 4-carboxy-2-hydroxymuconate semialdehyde.
  • COFACTOR: Fe(2+) ion.
  • SUBUNIT: Composed of two subunits (alpha and beta) in a 1:1 ratio.
Copyright
Copyrighted by the UniProt Consortium, see http://www.uniprot.org/terms. Distributed under the Creative Commons Attribution-NoDerivs License.
Cross-references
Sequence databases
EMBL
M34835; AAA17728.1; -; Unassigned_DNA.[EMBL / GenBank / DDBJ] [CoDingSequence]
PIR B35271; B35271.
3D structure databases
PDB
1B4U; X-ray; 2.20 A; B/D=1-302.[ExPASy / RCSB / EBI]
1BOU; X-ray; 2.20 A; B/D=1-302.[ExPASy / RCSB / EBI]
Detailed list of linked structures.
PDBsum 1B4U; -.
1BOU; -.
ModBase P22636.
Ontologies
GO
GO:0009055; Molecular function: electron carrier activity (inferred from electronic annotation from InterPro).
GO:0008198; Molecular function: ferrous iron binding (inferred from electronic annotation from InterPro).
GO:0018579; Molecular function: protocatechuate 4,5-dioxygenase activity (inferred from electronic annotation from EC).
GO:0019439; Biological process: aromatic compound catabolic process (inferred from electronic annotation from UniProtKB-KW).
GO:0055114; Biological process: oxidation reduction (inferred from electronic annotation from UniProtKB-KW).
QuickGo view.
Family and domain databases
InterPro IPR004183; Xdiol_dOase_3B.
Graphical view of domain structure.
Gene3D G3DSA:3.40.830.10; Xdiol_dOase_3B; 1.
Pfam PF02900; LigB; 1.
Pfam graphical view of domain structure.
ProtoNet P22636.
Other
UniRef View cluster of proteins with at least 50% / 90% / 100% identity.
Keywords
3D-structure; Aromatic hydrocarbons catabolism; Dioxygenase; Direct protein sequencing; Iron; Oxidoreductase.
Features
SEVIEWER logo Feature table viewer
KeyFrom   To Length Description FTId
CHAIN   1   302  302     Protocatechuate 4,5-dioxygenase beta chain. PRO_0000085101
STRAND   3    10  8      
HELIX   14    21  8      
TURN   28    30  3      
HELIX   31    44  14      
TURN   47    49  3      
STRAND   52    58  7      
STRAND   62    64  3      
STRAND   69    76  8      
STRAND   78    81  4      
STRAND   87    90  4      
HELIX   100   112  13      
STRAND   118   122  5      
HELIX   127   137  11      
STRAND   144   152  9      
STRAND   155   157  3      
HELIX   162   177  16      
STRAND   179   181  3      
STRAND   184   190  7      
TURN   200   203  4      
HELIX   207   219  13      
HELIX   221   224  4      
HELIX   229   236  8      
HELIX   240   243  4      
HELIX   244   251  8      
STRAND   257   268  12      
STRAND   271   280  10      
HELIX   281   283  3      
STRAND   289   296  8      
Sequence information
Length: 302 AA [This is the length of the unprocessed precursor] Molecular weight: 33292 Da [This is the MW of the unprocessed precursor] CRC64: 0552B3B0E59702E5 [This is a checksum on the sequence] 
        10         20         30         40         50         60 
MARVTTGITS SHIPALGAAI QTGTSDNDYW GPVFKGYQPI RDWIKQPGNM PDVVILVYND 

        70         80         90        100        110        120 
HASAFDMNII PTFAIGCAET FKPADEGWGP RPVPDVKGHP DLAWHIAQSL ILDEFDMTIM 

       130        140        150        160        170        180 
NQMDVDHGCT VPLSMIFGEP EEWPCKVIPF PVNVVTYPPP SGKRCFALGD SIRAAVESFP 

       190        200        210        220        230        240 
EDLNVHVWGT GGMSHQLQGP RAGLINKEFD LNFIDKLISD PEELSKMPHI QYLRESGSEG 

       250        260        270        280        290        300 
VELVMWLIMR GALPEKVRDL YTFYHIPASN TALGAMILQP EETAGTPLEP RKVMSGHSLA 


QA
P22636 in FASTA format

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View entry in raw text format (no links)
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