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UniProtKB/Swiss-Prot entry P22276


[Entry info] [Name and origin] [References] [Comments] [Cross-references] [Keywords] [Features] [Sequence] [Tools]

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Entry information
Entry name RPC2_YEAST
Primary accession number P22276
Secondary accession number Q12696
Integrated into Swiss-Prot on August 1, 1991
Sequence was last modified on November 1, 1997 (Sequence version 2)
Annotations were last modified on    November 25, 2008 (Entry version 71)
Name and origin of the protein
Protein name DNA-directed RNA polymerase III subunit RPC2
Synonyms RNA polymerase III subunit C2
EC 2.7.7.6
DNA-directed RNA polymerase III 130 kDa polypeptide
C128
Gene name
Name: RET1
Synonyms: RPC128, RPC2
OrderedLocusNames: YOR207C
From
Saccharomyces cerevisiae (Baker's yeast) [TaxID: 4932] 
Taxonomy Eukaryota; Fungi; Dikarya; Ascomycota; Saccharomycotina; Saccharomycetes; Saccharomycetales; Saccharomycetaceae; Saccharomyces.
Protein existence 1: Evidence at protein level;
References
[1]
NUCLEOTIDE SEQUENCE [GENOMIC DNA].
PubMed=2005101 [NCBI, ExPASy, EBI, Israel, Japan]
James P., Whelen S., Hall B.D.;
"The RET1 gene of yeast encodes the second-largest subunit of RNA polymerase III. Structural analysis of the wild-type and ret1-1 mutant alleles.";
J. Biol. Chem. 266:5616-5624(1991).
[2]
NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
STRAIN=ATCC 96604 / S288c / FY1679;
PubMed=9169874 [NCBI, ExPASy, EBI, Israel, Japan]
Dujon B., Albermann K., Aldea M., Alexandraki D., Ansorge W., Arino J., Benes V., Bohn C., Bolotin-Fukuhara M., Bordonne R., Boyer J., Camasses A., Casamayor A., Casas C., Cheret G., Cziepluch C., Daignan-Fornier B., Dang V.-D., de Haan M., Delius H., Durand P., Fairhead C., Feldmann H., Gaillon L., Galisson F., Gamo F.-J., Gancedo C., Goffeau A., Goulding S.E., Grivell L.A., Habbig B., Hand N.J., Hani J., Hattenhorst U., Hebling U., Hernando Y., Herrero E., Heumann K., Hiesel R., Hilger F., Hofmann B., Hollenberg C.P., Hughes B., Jauniaux J.-C., Kalogeropoulos A., Katsoulou C., Kordes E., Lafuente M.J., Landt O., Louis E.J., Maarse A.C., Madania A., Mannhaupt G., Marck C., Martin R.P., Mewes H.-W., Michaux G., Paces V., Parle-McDermott A.G., Pearson B.M., Perrin A., Pettersson B., Poch O., Pohl T.M., Poirey R., Portetelle D., Pujol A., Purnelle B., Ramezani Rad M., Rechmann S., Schwager C., Schweizer M., Sor F., Sterky F., Tarassov I.A., Teodoru C., Tettelin H., Thierry A., Tobiasch E., Tzermia M., Uhlen M., Unseld M., Valens M., Vandenbol M., Vetter I., Vlcek C., Voet M., Volckaert G., Voss H., Wambutt R., Wedler H., Wiemann S., Winsor B., Wolfe K.H., Zollner A., Zumstein E., Kleine K.;
"The nucleotide sequence of Saccharomyces cerevisiae chromosome XV.";
Nature 387:98-102(1997).
[3]
REVIEW ON THE RNA POL III COMPLEX.
DOI=10.1101/sqb.1998.63.381; PubMed=10384303 [NCBI, ExPASy, EBI, Israel, Japan]
Chedin S., Ferri M.L., Peyroche G., Andrau J.-C., Jourdain S., Lefebvre O., Werner M., Carles C., Sentenac A.;
"The yeast RNA polymerase III transcription machinery: a paradigm for eukaryotic gene activation.";
Cold Spring Harb. Symp. Quant. Biol. 63:381-389(1998).
[4]
SUBCELLULAR LOCATION [LARGE SCALE ANALYSIS].
DOI=10.1038/nature02026; PubMed=14562095 [NCBI, ExPASy, EBI, Israel, Japan]
Huh W.-K., Falvo J.V., Gerke L.C., Carroll A.S., Howson R.W., Weissman J.S., O'Shea E.K.;
"Global analysis of protein localization in budding yeast.";
Nature 425:686-691(2003).
[5]
LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
DOI=10.1038/nature02046; PubMed=14562106 [NCBI, ExPASy, EBI, Israel, Japan]
Ghaemmaghami S., Huh W.-K., Bower K., Howson R.W., Belle A., Dephoure N., O'Shea E.K., Weissman J.S.;
"Global analysis of protein expression in yeast.";
Nature 425:737-741(2003).
[6]
3D-STRUCTURE MODELING OF THE POL III CORE COMPLEX.
DOI=10.1016/j.molcel.2006.05.013; PubMed=16818233 [NCBI, ExPASy, EBI, Israel, Japan]
Jasiak A.J., Armache K.J., Martens B., Jansen R.P., Cramer P.;
"Structural biology of RNA polymerase III: subcomplex C17/25 X-ray structure and 11 subunit enzyme model.";
Mol. Cell 23:71-81(2006).
Comments
  • FUNCTION: DNA-dependent RNA polymerase catalyzes the transcription of DNA into RNA using the four ribonucleoside triphosphates as substrates. Second largest core component of RNA polymerase III which synthesizes small RNAs, such as 5S rRNA and tRNAs. Proposed to contribute to the polymerase catalytic activity and forms the polymerase active center together with the largest subunit. Pol III is composed of mobile elements and RPC2 is part of the core element with the central large cleft and probably a clamp element that moves to open and close the cleft (By similarity).
  • CATALYTIC ACTIVITY: Nucleoside triphosphate + RNA(n) = diphosphate + RNA(n+1).
  • SUBUNIT: Component of the RNA polymerase III (Pol III) complex consisting of 17 subunits.
  • SUBCELLULAR LOCATION: Nucleus.
  • MISCELLANEOUS: Present with 1950 molecules/cell in log phase SD medium.
  • SIMILARITY: Belongs to the RNA polymerase beta chain family.
Copyright
Copyrighted by the UniProt Consortium, see http://www.uniprot.org/terms. Distributed under the Creative Commons Attribution-NoDerivs License.
Cross-references
Sequence databases
EMBL
M38723; AAB59324.1; -; Genomic_DNA.[EMBL / GenBank / DDBJ] [CoDingSequence]
Z75115; CAA99422.1; -; Genomic_DNA.[EMBL / GenBank / DDBJ] [CoDingSequence]
PIR S67099; S67099.
RefSeq NP_014850.1; -.
3D structure databases
HSSP P08518; 1I50. [HSSP ENTRY / PDB]
ModBase P22276.
Protein-protein interaction databases
DIP DIP:997N; -.
IntAct P22276; -.
Organism-specific databases
CYGD YOR207c; -.
SGD S000005733; RET1.
Yeast-GFP YOR207C.
Gene expression databases
ArrayExpress P22276; -.
GermOnline YOR207C; Saccharomyces cerevisiae.
Ontologies
GO
GO:0005666; Cellular component: DNA-directed RNA polymerase III complex (traceable author statement from SGD).
GO:0003677; Molecular function: DNA binding (inferred from electronic annotation from InterPro).
GO:0003899; Molecular function: DNA-directed RNA polymerase activity (inferred from electronic annotation from InterPro).
GO:0008270; Molecular function: zinc ion binding (inferred from electronic annotation from UniProtKB-KW).
GO:0006350; Biological process: transcription (inferred from electronic annotation from InterPro).
QuickGo view.
Family and domain databases
InterPro IPR015712; DNA-dir_RNA_pol_su2.
IPR007120; DNA-dir_RNA_pol_su2_6.
IPR007121; RNA_pol_bsu_CS.
IPR007644; RNA_pol_bsu_protrusion.
IPR007642; RNA_pol_Rpb2_2.
IPR007645; RNA_pol_Rpb2_3.
IPR007646; RNA_pol_Rpb2_4.
IPR007647; RNA_pol_Rpb2_5.
IPR007641; RNA_pol_Rpb2_7.
Graphical view of domain structure.
PANTHER PTHR20856; RNA_pol_I_sub2; 1.
Pfam PF04563; RNA_pol_Rpb2_1; 1.
PF04561; RNA_pol_Rpb2_2; 1.
PF04565; RNA_pol_Rpb2_3; 1.
PF04566; RNA_pol_Rpb2_4; 1.
PF04567; RNA_pol_Rpb2_5; 1.
PF00562; RNA_pol_Rpb2_6; 1.
PF04560; RNA_pol_Rpb2_7; 1.
Pfam graphical view of domain structure.
PROSITE PS01166; RNA_POL_BETA; 1.
ProtoNet P22276.
Proteomic databases
PeptideAtlas P22276; -.
Genome annotation databases
Ensembl YOR207C; Saccharomyces cerevisiae. [Contig view]
GeneID 854382; -.
GenomeReviews Y13140_GR; YOR207C.
KEGG sce:YOR207C; -.
NMPDR fig|4932.3.peg.5962; -.
Phylogenomic databases
HOGENOM P22276; -.
Other
LinkHub P22276; -.
NextBio 976523; -.
UniRef View cluster of proteins with at least 50% / 90% / 100% identity.
Keywords
Complete proteome; DNA-directed RNA polymerase; Metal-binding; Nucleotidyltransferase; Nucleus; Transcription; Transferase; Zinc; Zinc-finger.
Features
SEVIEWER logo Feature table viewer FT aligner logo Feature aligner
KeyFrom    To Length Description FTId
CHAIN   1   1149  1149     DNA-directed RNA polymerase III subunit RPC2. PRO_0000048096
ZN_FING   1095   1110  16     C4-type (By similarity). 
METAL   1095   1095        Zinc (By similarity). 
METAL   1098   1098        Zinc (By similarity). 
METAL   1107   1107        Zinc (By similarity). 
METAL   1110   1110        Zinc (By similarity). 
CONFLICT   213    213        K -> E (in Ref. 1; AAB59324). 
Sequence information
Length: 1149 AA [This is the length of the unprocessed precursor] Molecular weight: 129456 Da [This is the MW of the unprocessed precursor] CRC64: 6AC52354F34CF090 [This is a checksum on the sequence]
        10         20         30         40         50         60 
MVAATKRRKT HIHKHVKDEA FDDLLKPVYK GKKLTDEINT AQDKWHLLPA FLKVKGLVKQ 

        70         80         90        100        110        120 
HLDSFNYFVD TDLKKIIKAN QLILSDVDPE FYLKYVDIRV GKKSSSSTKD YLTPPHECRL 

       130        140        150        160        170        180 
RDMTYSAPIY VDIEYTRGRN IIMHKDVEIG RMPIMLRSNK CILYDADESK MAKLNECPLD 

       190        200        210        220        230        240 
PGGYFIVNGT EKVILVQEQL SKNRIIVEAD EKKGIVQASV TSSTHERKSK TYVITKNGKI 

       250        260        270        280        290        300 
YLKHNSIAEE IPIAIVLKAC GILSDLEIMQ LVCGNDSSYQ DIFAVNLEES SKLDIYTQQQ 

       310        320        330        340        350        360 
ALEYIGAKVK TMRRQKLTIL QEGIEAIATT VIAHLTVEAL DFREKALYIA MMTRRVVMAM 

       370        380        390        400        410        420 
YNPKMIDDRD YVGNKRLELA GQLISLLFED LFKKFNNDFK LSIDKVLKKP NRAMEYDALL 

       430        440        450        460        470        480 
SINVHSNNIT SGLNRAISTG NWSLKRFKME RAGVTHVLSR LSYISALGMM TRISSQFEKS 

       490        500        510        520        530        540 
RKVSGPRALQ PSQFGMLCTA DTPEGEACGL VKNLALMTHI TTDDEEEPIK KLCYVLGVED 

       550        560        570        580        590        600 
ITLIDSASLH LNYGVYLNGT LIGSIRFPTK FVTQFRHLRR TGKVSEFISI YSNSHQMAVH 

       610        620        630        640        650        660 
IATDGGRICR PLIIVSDGQS RVKDIHLRKL LDGELDFDDF LKLGLVEYLD VNEENDSYIA 

       670        680        690        700        710        720 
LYEKDIVPSM THLEIEPFTI LGAVAGLIPY PHHNQSPRNT YQCAMGKQAI GAIAYNQFKR 

       730        740        750        760        770        780 
IDTLLYLMTY PQQPMVKTKT IELIDYDKLP AGQNATVAVM SYSGYDIEDA LVLNKSSIDR 

       790        800        810        820        830        840 
GFGRCETRRK TTTVLKRYAN HTQDIIGGMR VDENGDPIWQ HQSLGPDGLG EVGMKVQSGQ 

       850        860        870        880        890        900 
IYINKSVPTN SADAPNPNNV NVQTQYREAP VIYRGPEPSH IDQVMMSVSD NDQALIKVLL 

       910        920        930        940        950        960 
RQNRRPELGD KFSSRHGQKG VCGIIVKQED MPFNDQGIVP DIIMNPHGFP SRMTVGKMIE 

       970        980        990       1000       1010       1020 
LISGKAGVLN GTLEYGTCFG GSKLEDMSKI LVDQGFNYSG KDMLYSGITG ECLQAYIFFG 

      1030       1040       1050       1060       1070       1080 
PIYYQKLKHM VLDKMHARAR GPRAVLTRQP TEGRSRDGGL RLGEMERDCV IAYGASQLLL 

      1090       1100       1110       1120       1130       1140 
ERLMISSDAF EVDVCDKCGL MGYSGWCTTC KSAENIIKMT IPYAAKLLFQ ELLSMNIAPR 


LRLEDIFQQ 

P22276 in FASTA format

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