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UniProtKB/Swiss-Prot entry P22200

[Entry info] [Name and origin] [References] [Comments] [Cross-references] [Keywords] [Features] [Sequence] [Tools]

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Entry information
Entry name KPYC_SOLTU
Primary accession number P22200
Secondary accession numbers None
Integrated into Swiss-Prot on August 1, 1991
Sequence was last modified on August 1, 1991 (Sequence version 1)
Annotations were last modified on    November 25, 2008 (Entry version 64)
Name and origin of the protein
Protein name Pyruvate kinase, cytosolic isozyme
Synonyms PK
EC 2.7.1.40
Gene name None
From
Solanum tuberosum (Potato) [TaxID: 4113] 
Taxonomy Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta; Spermatophyta; Magnoliophyta; eudicotyledons; core eudicotyledons; asterids; lamiids; Solanales; Solanaceae; Solanoideae; Solaneae; Solanum.
Protein existence 2: Evidence at transcript level;
References
[1]
 NUCLEOTIDE SEQUENCE [MRNA].
STRAIN=cv. Kennebec;
TISSUE=Tuber;
DOI=10.1007/BF00017842; PubMed=2102383 [NCBI, ExPASy, EBI, Israel, Japan]
Blakeley S.D., Plaxton W.C., Dennis D.T.;
"Cloning and characterization of a cDNA for the cytosolic isozyme of plant pyruvate kinase: the relationship between the plant and non-plant enzyme.";
Plant Mol. Biol. 15:665-669(1990).
[2]
 NUCLEOTIDE SEQUENCE [MRNA].
DOI=10.1016/0378-1119(92)90213-9; PubMed=1487141 [NCBI, ExPASy, EBI, Israel, Japan]
Cole K.P., Blakeley S.D., Dennis D.T.;
"Structure of the gene encoding potato cytosolic pyruvate kinase.";
Gene 122:255-261(1992).
Comments
Copyright
Copyrighted by the UniProt Consortium, see http://www.uniprot.org/terms. Distributed under the Creative Commons Attribution-NoDerivs License.
Cross-references
Sequence databases
EMBL
X53688; CAA37727.1; -; mRNA.[EMBL / GenBank / DDBJ] [CoDingSequence]
PIR JC1481; JC1481.
3D structure databases
HSSP P14178; 1E0T. [HSSP ENTRY / PDB]
ModBase P22200.
Ontologies
GO
GO:0005737; Cellular component: cytoplasm (inferred from electronic annotation from UniProtKB-KW).
GO:0000287; Molecular function: magnesium ion binding (inferred from electronic annotation from InterPro).
GO:0030955; Molecular function: potassium ion binding (inferred from electronic annotation from InterPro).
GO:0004743; Molecular function: pyruvate kinase activity (inferred from electronic annotation from InterPro).
GO:0006096; Biological process: glycolysis (inferred from electronic annotation from InterPro).
QuickGo view.
Family and domain databases
InterPro IPR001697; Pyr_Knase.
IPR015813; Pyrv/PenolPyrv_Kinase_cat.
IPR015794; Pyrv_Knase_a/b.
IPR015793; Pyrv_Knase_brl.
Graphical view of domain structure.
Gene3D G3DSA:3.20.20.60; Pyrv/PenolPyrv_Kinase_cat; 1.
G3DSA:3.40.1380.20; Pyrv_Knase_a/b; 1.
PANTHER PTHR11817; Pyruvate_kinase; 1.
Pfam PF00224; PK; 1.
PF02887; PK_C; 1.
Pfam graphical view of domain structure.
PRINTS PR01050; PYRUVTKNASE.
ProDom PD001009; Pyruvate_kinase; 2.
[Domain structure / List of seq. sharing at least 1 domain]
TIGRFAMs TIGR01064; pyruv_kin; 1.
PROSITE PS00110; PYRUVATE_KINASE; 1.
ProtoNet P22200.
Other
UniRef View cluster of proteins with at least 50% / 90% / 100% identity.
Keywords
Cytoplasm; Glycolysis; Kinase; Magnesium; Metal-binding; Pyruvate; Transferase.
Features
SEVIEWER logo Feature table viewer
KeyFrom   To Length Description FTId
CHAIN   1   510  510     Pyruvate kinase, cytosolic isozyme. PRO_0000112124
ACT_SITE   240   240        By similarity. 
METAL   242   242        Magnesium (Potential). 
METAL   263   263        Magnesium (Potential). 
METAL   264   264        Magnesium (Potential). 
VARIANT   133   133  1     M -> V. 
VARIANT   169   169  1     T -> S. 
VARIANT   227   227  1     V -> A. 
VARIANT   309   309  1     A -> R. 
Sequence information
Length: 510 AA [This is the length of the unprocessed precursor] Molecular weight: 55170 Da [This is the MW of the unprocessed precursor] CRC64: 1752C9ED920F2854 [This is a checksum on the sequence] 
        10         20         30         40         50         60 
MANIDIAGIM KDLPNDGRIP KTKIVCTLGP SSRTVPMLEK LLRAGMNVAR FNFSHGTHEY 

        70         80         90        100        110        120 
HQETLDNLKI AMQNTQILCA VMLDTKGPEI RTGFLTDGKP IQLKEGQEIT VSTDYTIKGN 

       130        140        150        160        170        180 
EEMISMSYKK LVMDLKPGNT ILCADGTITL TVLSCDPPSG TVRCRCENTA TLGERKNVNL 

       190        200        210        220        230        240 
PGVVVDLPTL TEKDKEDILE WGVPNNIDMI ALSFVRKGSD LVNVRKVLGP HAKRIQLMSK 

       250        260        270        280        290        300 
VENQEGVINF DEILRETDSF MVARGDLGME IPVEKIFLAQ KMMIYKCNLA GKAVVTATQM 

       310        320        330        340        350        360 
LESMIKSPAP TRAEATDVAN AVLDGTDCVM LSGESAAGAY PELAVKIMSR ICIEAESSLD 

       370        380        390        400        410        420 
NEAIFKEMIR CTPLPMSPLE SLASSAVRTA NKARAKLIVV LTRGGSTAKL VAKYRPAVPI 

       430        440        450        460        470        480 
LSVVVPVLTT DSFDWSISDE TPARHSLVYR GLIPLLGEGS AKATDSESTE VILEAALKSA 

       490        500        510 
VTRGLCKPGD AVVALHRIGS ASVIKICVVK
P22200 in FASTA format

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