ID   3BHS2_RAT               Reviewed;         373 AA.
AC   P22072;
DT   01-AUG-1991, integrated into UniProtKB/Swiss-Prot.
DT   23-JAN-2007, sequence version 3.
DT   25-NOV-2008, entry version 71.
DE   RecName: Full=3 beta-hydroxysteroid dehydrogenase/Delta 5-->4-isomerase type 2;
DE   AltName: Full=3-beta-HSD II;
DE   Includes:
DE     RecName: Full=3-beta-hydroxy-Delta(5)-steroid dehydrogenase;
DE              EC=1.1.1.145;
DE     AltName: Full=3-beta-hydroxy-5-ene steroid dehydrogenase;
DE     AltName: Full=Progesterone reductase;
DE   Includes:
DE     RecName: Full=Steroid Delta-isomerase;
DE              EC=5.3.3.1;
DE     AltName: Full=Delta-5-3-ketosteroid isomerase;
GN   Name=Hsd3b;
OS   Rattus norvegicus (Rat).
OC   Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
OC   Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Sciurognathi;
OC   Muroidea; Muridae; Murinae; Rattus.
OX   NCBI_TaxID=10116;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [MRNA].
RX   MEDLINE=91093186; PubMed=1985917;
RA   Zhao H.-F., Labrie C., Simard J., de Launoit Y., Trudel C., Martel C.,
RA   Rheaume E., Dupont E., Luu-The V., Pelletier G., Labrie F.;
RT   "Characterization of rat 3 beta-hydroxysteroid dehydrogenase/delta 5-
RT   delta 4 isomerase cDNAs and differential tissue-specific expression of
RT   the corresponding mRNAs in steroidogenic and peripheral tissues.";
RL   J. Biol. Chem. 266:583-593(1991).
RN   [2]
RP   NUCLEOTIDE SEQUENCE [MRNA].
RC   TISSUE=Liver;
RX   MEDLINE=92049388; PubMed=1944305;
RA   Naville D., Keeney D.S., Jenkin G., Murry B.A., Head J.R., Mason J.I.;
RT   "Regulation of expression of male-specific rat liver microsomal 3
RT   beta-hydroxysteroid dehydrogenase.";
RL   Mol. Endocrinol. 5:1090-1100(1991).
CC   -!- FUNCTION: 3-beta-HSD is a bifunctional enzyme, that catalyzes the
CC       oxidative conversion of Delta(5)-ene-3-beta-hydroxy steroid, and
CC       the oxidative conversion of ketosteroids. The 3-beta-HSD enzymatic
CC       system plays a crucial role in the biosynthesis of all classes of
CC       hormonal steroids.
CC   -!- CATALYTIC ACTIVITY: A 3-beta-hydroxy-Delta(5)-steroid + NAD(+) = a
CC       3-oxo-Delta(5)-steroid + NADH.
CC   -!- CATALYTIC ACTIVITY: A 3-oxo-Delta(5)-steroid = a 3-oxo-Delta(4)-
CC       steroid.
CC   -!- PATHWAY: Lipid metabolism; steroid biosynthesis.
CC   -!- SUBCELLULAR LOCATION: Endoplasmic reticulum membrane; Single-pass
CC       membrane protein. Mitochondrion membrane; Single-pass membrane
CC       protein.
CC   -!- TISSUE SPECIFICITY: Adrenal glands, testes and ovaries.
CC   -!- SIMILARITY: Belongs to the 3-beta-HSD family.
CC   -!- CAUTION: Rat 3-beta-HSD type II may possess only one transmembrane
CC       domain.
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DR   EMBL; M38179; AAA63475.1; -; mRNA.
DR   EMBL; S63167; AAB20228.1; -; mRNA.
DR   PIR; A40378; DERTHM.
DR   PIR; B39051; DERTH2.
DR   RefSeq; NP_001036084.1; -.
DR   UniGene; Rn.128814; -.
DR   PhosphoSite; P22072; -.
DR   Ensembl; ENSRNOG00000019454; Rattus norvegicus.
DR   GeneID; 682974; -.
DR   KEGG; rno:682974; -.
DR   RGD; 1592771; Hsd3b.
DR   HOVERGEN; P22072; -.
DR   NextBio; 724140; -.
DR   ArrayExpress; P22072; -.
DR   GermOnline; ENSRNOG00000019454; Rattus norvegicus.
DR   GO; GO:0005789; C:endoplasmic reticulum membrane; IEA:UniProtKB-SubCell.
DR   GO; GO:0016021; C:integral to membrane; IEA:UniProtKB-KW.
DR   GO; GO:0031966; C:mitochondrial membrane; IEA:UniProtKB-SubCell.
DR   GO; GO:0003854; F:3-beta-hydroxy-delta5-steroid dehydrogenase...; IEA:InterPro.
DR   GO; GO:0005488; F:binding; IEA:InterPro.
DR   GO; GO:0004769; F:steroid delta-isomerase activity; IEA:EC.
DR   GO; GO:0006700; P:C21-steroid hormone biosynthetic process; IEA:UniProtKB-KW.
DR   GO; GO:0055114; P:oxidation reduction; IEA:UniProtKB-KW.
DR   InterPro; IPR002225; 3Beta_OHSteriod_DHase/Estase.
DR   InterPro; IPR016040; NAD(P)-bd.
DR   Gene3D; G3DSA:3.40.50.720; NAD(P)-bd; 1.
DR   Pfam; PF01073; 3Beta_HSD; 1.
PE   2: Evidence at transcript level;
KW   Endoplasmic reticulum; Isomerase; Membrane; Mitochondrion;
KW   Multifunctional enzyme; NAD; Oxidoreductase; Steroidogenesis;
KW   Transmembrane.
FT   INIT_MET      1      1       Removed (By similarity).
FT   CHAIN         2    373       3 beta-hydroxysteroid dehydrogenase/Delta
FT                                5-->4-isomerase type 2.
FT                                /FTId=PRO_0000087788.
FT   TRANSMEM    288    308       Potential.
FT   CONFLICT    115    115       A -> T (in Ref. 2; AAB20228).
FT   CONFLICT    165    165       A -> S (in Ref. 2; AAB20228).
FT   CONFLICT    341    341       E -> V (in Ref. 2; AAB20228).
SQ   SEQUENCE   373 AA;  42277 MW;  0F922E9632DE9526 CRC64;
     MPGWSCLVTG AGGFVGQRII RMLVQEKELQ EVRALDKVFR PETKEEFSKL QTKAKVTMLE
     GDILDAQYLR RACQGISVVI HTASVMDFSR VLPRQTILDV NLKGTQNLLE AGIHASVPAF
     IYCSTVDVAG PNSYKKTILN GREEEHHEST WSNPYPYSKK MAEKAVLAAN GSILKNGGTL
     HTCALRPMYI YGERGQFLSR IIIMALKNKG VLNVTGKFSI VNPVYVGNVA WAHILAARGL
     RDPKKSQNIQ GQFYYISDDT PHQSYDDLNC TLSKEWGLRL DSSWSLPLPL LYWLAFLLET
     VSFLLRPFYN YRPPFNCHLV TLSNSKFTFS YKKAQRDLGY EPLVSWEEAK QKTSEWIGTL
     VEQHRETLDT KSQ
//