[1]	NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. STRAIN=Brown Norway; Mural R.J., Adams M.D., Myers E.W., Smith H.O., Venter J.C.; Submitted (AUG-2005) to the EMBL/GenBank/DDBJ databases.
[2]	NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. TISSUE=Placenta; DOI=10.1101/gr.2596504; PubMed=15489334 [NCBI, ExPASy, EBI, Israel, Japan] The MGC Project Team; "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."; Genome Res. 14:2121-2127(2004).
[3]	NUCLEOTIDE SEQUENCE [MRNA] OF 26-257. TISSUE=Liver; DOI=10.1093/molbev/msl027; PubMed=16751257 [NCBI, ExPASy, EBI, Israel, Japan] Kullberg M., Nilsson M.A., Arnason U., Harley E.H., Janke A.; "Housekeeping genes for phylogenetic analysis of eutherian relationships."; Mol. Biol. Evol. 23:1493-1503(2006).
[4]	NUCLEOTIDE SEQUENCE [MRNA] OF 109-251. TISSUE=Brain cortex; PubMed=2494655 [NCBI, ExPASy, EBI, Israel, Japan] Gould S.J., Subramani S., Scheffler I.E.; "Use of the DNA polymerase chain reaction for homology probing: isolation of partial cDNA or genomic clones encoding the iron-sulfur protein of succinate dehydrogenase from several species."; Proc. Natl. Acad. Sci. U.S.A. 86:1934-1938(1989).
[5]	ERRATUM. Gould S.J., Subramani S., Scheffler I.E.; Proc. Natl. Acad. Sci. U.S.A. 90:2556-2556(1993).

Comments

FUNCTION: Iron-sulfur protein (IP) subunit of succinate dehydrogenase (SDH) that is involved in complex II of the mitochondrial electron transport chain and is responsible for transferring electrons from succinate to ubiquinone (coenzyme Q) (By similarity).
CATALYTIC ACTIVITY: Succinate + ubiquinone = fumarate + ubiquinol.
COFACTOR: Binds 1 2Fe-2S cluster (By similarity).
COFACTOR: Binds 1 3Fe-4S cluster (By similarity).
COFACTOR: Binds 1 4Fe-4S cluster (By similarity).
PATHWAY: Carbohydrate metabolism; tricarboxylic acid cycle .
SUBUNIT: Component of complex II composed of four subunits: the flavoprotein (FP) SDHA, iron-sulfur protein (IP) SDHB, and a cytochrome b560 composed of SDHC and SDHD (By similarity).
SUBCELLULAR LOCATION: Mitochondrion inner membrane; Peripheral membrane protein; Matrix side (By similarity).
SIMILARITY: Belongs to the succinate dehydrogenase/fumarate reductase iron-sulfur protein family.
SIMILARITY: Contains 1 2Fe-2S ferredoxin-type domain.
SIMILARITY: Contains 1 4Fe-4S ferredoxin-type domain.

Copyright

Copyrighted by the UniProt Consortium, see http://www.uniprot.org/terms. Distributed under the Creative Commons Attribution-NoDerivs License.

Cross-references

Sequence databases

EMBL

CH473968; EDL80954.1; -; Genomic_DNA.	[EMBL / GenBank / DDBJ] [CoDingSequence]
BC158620; AAI58621.1; -; mRNA.	[EMBL / GenBank / DDBJ] [CoDingSequence]
DQ403001; ABD77134.1; -; mRNA.	[EMBL / GenBank / DDBJ] [CoDingSequence]

PIR

B32394; B32394.

RefSeq

XP_001071846.1; -.
XP_216558.3; -.

UniGene

Rn.3902

3D structure databases

HSSP

P07014; 1NEK. [HSSP ENTRY / PDB]

SMR

P21913; 1-143.

ModBase

P21913.

Organism-specific databases

RGD

1308598; Sdhb.

Gene expression databases

ArrayExpress

P21913; -.

GermOnline

ENSRNOG00000007967; Rattus norvegicus.

Ontologies

GO:0005743;	Cellular component: mitochondrial inner membrane (inferred from electronic annotation from UniProtKB-KW).
GO:0051537;	Molecular function: 2 iron, 2 sulfur cluster binding (inferred from electronic annotation from UniProtKB-KW).
GO:0051538;	Molecular function: 3 iron, 4 sulfur cluster binding (inferred from electronic annotation from UniProtKB-KW).
GO:0051539;	Molecular function: 4 iron, 4 sulfur cluster binding (inferred from electronic annotation from UniProtKB-KW).
GO:0009055;	Molecular function: electron carrier activity (inferred from electronic annotation from InterPro).
GO:0005506;	Molecular function: iron ion binding (inferred from electronic annotation from UniProtKB-KW).
GO:0008177;	Molecular function: succinate dehydrogenase (ubiquinone) activity (inferred from electronic annotation from EC).
GO:0022900;	Biological process: electron transport chain (inferred from electronic annotation from UniProtKB-KW).
GO:0006810;	Biological process: transport (inferred from electronic annotation from UniProtKB-KW).
GO:0006099;	Biological process: tricarboxylic acid cycle (inferred from electronic annotation from InterPro).
QuickGo view.

Family and domain databases

InterPro

IPR006058; 2Fe2S_fd_BS.
IPR001450; 4Fe4S_Fe_S_bd.
IPR012675; b-grasp_ferredoxin-like.
IPR001041; Ferredoxin.
IPR012285; Fum_reductase_C.
IPR004489; Succ_DHase/fum_Rdtase_Fe-S.
Graphical view of domain structure.

Gene3D

G3DSA:3.10.20.30; Ferredoxin_fold; 1.
G3DSA:1.10.1060.10; Fum_reductase_C; 1.

TIGRFAMs

TIGR00384; dhsB; 1.

PROSITE

PS00197; 2FE2S_FER_1; 1.
PS51085; 2FE2S_FER_2; 1.
PS00198; 4FE4S_FER_1; 1.
PS51379; 4FE4S_FER_2; 1.
PROSITE graphical view of domain structure (profiles).

ProtoNet

P21913.

Genome annotation databases

Ensembl

ENSRNOG00000007967; Rattus norvegicus. [Contig view]

GeneID

298596; -.

Phylogenomic databases

HOVERGEN

P21913; -.

Other

UniRef

View cluster of proteins with at least 50% / 90% / 100% identity.

Keywords

2Fe-2S; 3Fe-4S; 4Fe-4S; Electron transport; Iron; Iron-sulfur; Membrane; Metal-binding; Mitochondrion; Mitochondrion inner membrane; Oxidoreductase; Transit peptide; Transport; Tricarboxylic acid cycle.

Features

Feature table viewer

Feature aligner

`Key`	`From To`	`Length`	`Description`	`FTId`
`TRANSIT`	`1 30`	`30`	`Mitochondrion (By similarity).`
`CHAIN`	`31 282`	`252`	`Succinate dehydrogenase [ubiquinone] iron-sulfur subunit, mitochondrial.`	`PRO_0000158693`
`DOMAIN`	`56 135`	`80`	`2Fe-2S ferredoxin-type.`
`DOMAIN`	`178 208`	`31`	`4Fe-4S ferredoxin-type.`
`METAL`	`95 95`		`Iron-sulfur 1 (2Fe-2S) (By similarity).`
`METAL`	`100 100`		`Iron-sulfur 1 (2Fe-2S) (By similarity).`
`METAL`	`103 103`		`Iron-sulfur 1 (2Fe-2S) (By similarity).`
`METAL`	`115 115`		`Iron-sulfur 1 (2Fe-2S) (By similarity).`
`METAL`	`188 188`		`Iron-sulfur 2 (4Fe-4S) (By similarity).`
`METAL`	`191 191`		`Iron-sulfur 2 (4Fe-4S) (By similarity).`
`METAL`	`194 194`		`Iron-sulfur 2 (4Fe-4S) (By similarity).`
`METAL`	`198 198`		`Iron-sulfur 3 (3Fe-4S) (By similarity).`
`METAL`	`245 245`		`Iron-sulfur 3 (3Fe-4S) (By similarity).`
`METAL`	`251 251`		`Iron-sulfur 3 (3Fe-4S) (By similarity).`
`METAL`	`255 255`		`Iron-sulfur 2 (4Fe-4S) (By similarity).`
`BINDING`	`203 203`		`Ubiquinone; shared with DHSD (By similarity).`
`CONFLICT`	`122 124`		`DLG -> NLN (in Ref. 4).`
`CONFLICT`	`153 153`		`K -> R (in Ref. 4).`
`CONFLICT`	`178 178`		`D -> E (in Ref. 4).`
`CONFLICT`	`227 227`		`E -> D (in Ref. 4).`

Sequence information

Length: 282 AA [This is the length of the unprocessed precursor]

Molecular weight: 31830 Da [This is the MW of the unprocessed precursor]

CRC64: 6600EBC8201529A9 [This is a checksum on the sequence]

        10         20         30         40         50         60 
MAAVVGVSLK RGFSATALGR VGLQFQACRE AQTAAAAAPR IKTFAIYRWD PDKAGDKPRM 

        70         80         90        100        110        120 
QTYKVDLNKC GPMVLDALIK IKNEIDSTLT FRRSCREGIC GSCAMNINGG NTLACTRRID 

       130        140        150        160        170        180 
TDLGKVSKIY PLPHMYVIKD LVPDLSNFYA QYKSIEPYLK KKDESQEGKQ QYLQSIEDRE 

       190        200        210        220        230        240 
KLDGLYECIL CACCSTSCPS YWWNGDKYLG PAVLMQAYRW MIDSRDEFTE ERLAKLQDPF 

       250        260        270        280 
SLYRCHTIMN CTQTCPKGLN PGKAIAEIKK MMATYKEKRA LA

P21913 in FASTA format

View entry in original UniProtKB/Swiss-Prot format
View entry in raw text format (no links)
Report form for errors/updates in this UniProtKB/Swiss-Prot entry

	BLAST submission on ExPASy/SIB or at NCBI (USA)		Sequence analysis tools: ProtParam, ProtScale, Compute pI/Mw, PeptideMass, PeptideCutter, Dotlet (Java)
	ScanProsite, MotifScan		Submit a homology modeling request to SWISS-MODEL
	NPSA Sequence analysis tools