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UniProtKB/Swiss-Prot entry P21844


[Entry info] [Name and origin] [References] [Comments] [Cross-references] [Keywords] [Features] [Sequence] [Tools]

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Entry information
Entry name CMA1_MOUSE
Primary accession number P21844
Secondary accession number Q9R1F0
Integrated into Swiss-Prot on May 1, 1991
Sequence was last modified on December 1, 1992 (Sequence version 2)
Annotations were last modified on    November 25, 2008 (Entry version 83)
Name and origin of the protein
Protein name Chymase [Precursor]
Synonyms EC 3.4.21.39
Alpha-chymase
Mast cell chymase 1
Mast cell protease I
Mast cell protease 5
MMCP-5
Gene name
Name: Cma1
Synonyms: Mcpt5
From
Mus musculus (Mouse) [TaxID: 10090] 
Taxonomy Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Sciurognathi; Muroidea; Muridae; Murinae; Mus.
Protein existence 1: Evidence at protein level;
References
[1]
NUCLEOTIDE SEQUENCE [MRNA].
PubMed=1939089 [NCBI, ExPASy, EBI, Israel, Japan]
McNeil H.P., Austen K.F., Somerville L.L., Gurish M.F., Stevens R.L.;
"Molecular cloning of the mouse mast cell protease-5 gene. A novel secretory granule protease expressed early in the differentiation of serosal mast cells.";
J. Biol. Chem. 266:20316-20322(1991).
[2]
NUCLEOTIDE SEQUENCE [MRNA].
STRAIN=Leaden X A1;
TISSUE=Mastocytoma;
PubMed=2060576 [NCBI, ExPASy, EBI, Israel, Japan]
Huang R., Blom T., Hellman L.;
"Cloning and structural analysis of MMCP-1, MMCP-4 and MMCP-5, three mouse mast cell-specific serine proteases.";
Eur. J. Immunol. 21:1611-1621(1991).
[3]
NUCLEOTIDE SEQUENCE [MRNA].
DOI=10.1016/0167-4838(92)90340-J; PubMed=1376147 [NCBI, ExPASy, EBI, Israel, Japan]
Chu W., Johnson D.A., Musich P.R.;
"Molecular cloning and characterization of mouse mast cell chymases.";
Biochim. Biophys. Acta 1121:83-87(1992).
[4]
NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 1-19.
STRAIN=129/Sv;
DOI=10.1007/s002510100316; PubMed=11398967 [NCBI, ExPASy, EBI, Israel, Japan]
Lunderius C., Hellman L.;
"Characterization of the gene encoding mouse mast cell protease 8 (mMCP-8), and a comparative analysis of hematopoietic serine protease genes.";
Immunogenetics 53:225-232(2001).
[5]
PROTEIN SEQUENCE OF 22-51.
PubMed=2326280 [NCBI, ExPASy, EBI, Israel, Japan]
Reynolds D.S., Stevens R.L., Lane W.S., Carr M.H., Austen K.F., Serafin W.E.;
"Different mouse mast cell populations express various combinations of at least six distinct mast cell serine proteases.";
Proc. Natl. Acad. Sci. U.S.A. 87:3230-3234(1990).
[6]
SUBCELLULAR LOCATION.
PubMed=1527387 [NCBI, ExPASy, EBI, Israel, Japan]
McNeil H.P., Frenkel D.P., Austen F., Friend D.S., Stevens R.L.;
"Translation and granule localization of mouse mast cell protease-5. Immunodetection with specific antipeptide Ig.";
J. Immunol. 149:2466-2472(1992).
Comments
Copyright
Copyrighted by the UniProt Consortium, see http://www.uniprot.org/terms. Distributed under the Creative Commons Attribution-NoDerivs License.
Cross-references
Sequence databases
EMBL
M73759; AAA40105.1; -; mRNA.[EMBL / GenBank / DDBJ] [CoDingSequence]
M73760; -; NOT_ANNOTATED_CDS; mRNA.[EMBL / GenBank / DDBJ]
X68805; CAA48705.1; ALT_INIT; mRNA.[EMBL / GenBank / DDBJ] [CoDingSequence]
M68898; AAA39492.1; -; mRNA.[EMBL / GenBank / DDBJ] [CoDingSequence]
AF119364; AAD43901.1; -; Genomic_DNA.[EMBL / GenBank / DDBJ] [CoDingSequence]
PIR S23504; S23504.
S26043; S26043.
RefSeq NP_034910.1; -.
UniGene Mm.1252
3D structure databases
HSSP P23946; 1NN6. [HSSP ENTRY / PDB]
SMR P21844; 22-247.
ModBase P21844.
Protein family/group databases
MEROPS S01.150; -.
PTM databases
PhosphoSite P21844; -.
Organism-specific databases
MGI MGI:96941; Cma1.
Gene expression databases
ArrayExpress P21844; -.
CleanEx MM_CMA1; -.
GermOnline ENSMUSG00000022225; Mus musculus.
Ontologies
GO
GO:0005576; Cellular component: extracellular region (inferred from electronic annotation from UniProtKB-KW).
GO:0005622; Cellular component: intracellular (inferred from direct assay from MGI).
GO:0004252; Molecular function: serine-type endopeptidase activity (inferred from electronic annotation from InterPro).
GO:0006508; Biological process: proteolysis (inferred from electronic annotation from InterPro).
QuickGo view.
Family and domain databases
InterPro IPR001254; Peptidase_S1_S6.
IPR001314; Peptidase_S1A.
Graphical view of domain structure.
Pfam PF00089; Trypsin; 1.
Pfam graphical view of domain structure.
PRINTS PR00722; CHYMOTRYPSIN.
SMART SM00020; Tryp_SPc; 1.
SMART graphical view of domain structure.
PROSITE PS50240; TRYPSIN_DOM; 1.
PS00134; TRYPSIN_HIS; 1.
PS00135; TRYPSIN_SER; 1.
PROSITE graphical view of domain structure (profiles).
ProtoNet P21844.
Genome annotation databases
Ensembl ENSMUSG00000022225; Mus musculus. [Contig view]
GeneID 17228; -.
KEGG mmu:17228; -.
Phylogenomic databases
HOVERGEN P21844; -.
Other
NextBio 291648; -.
SOURCE Cma1; Mus musculus.
UniRef View cluster of proteins with at least 50% / 90% / 100% identity.
Keywords
Direct protein sequencing; Glycoprotein; Hydrolase; Protease; Secreted; Serine protease; Signal; Zymogen.
Features
SEVIEWER logo Feature table viewer FT aligner logo Feature aligner
KeyFrom   To Length Description FTId
SIGNAL   1    19  19     Potential. 
PROPEP   20    21  2     Activation peptide. PRO_0000027455
CHAIN   22   247  226     Chymase. PRO_0000027456
DOMAIN   22   245  224     Peptidase S1. 
ACT_SITE   66    66        Charge relay system (By similarity). 
ACT_SITE   110   110        Charge relay system (By similarity). 
ACT_SITE   203   203        Charge relay system (By similarity). 
CARBOHYD   80    80        N-linked (GlcNAc...) (Potential). 
DISULFID   51    67        By similarity. 
DISULFID   144   209        By similarity. 
DISULFID   175   188        By similarity. 
CONFLICT   5     5        T -> A (in Ref. 1; AAA40105). 
CONFLICT   51    51        C -> R (in Ref. 5; AA sequence). 
CONFLICT   224   224        A -> R (in Ref. 3; AAA39492). 
Sequence information
Length: 247 AA [This is the length of the unprocessed precursor] Molecular weight: 27586 Da [This is the MW of the unprocessed precursor] CRC64: 24C290CF61237DC7 [This is a checksum on the sequence]
        10         20         30         40         50         60 
MHLLTLHLLL LLLGSSTKAG EIIGGTECIP HSRPYMAYLE IVTSENYLSA CSGFLIRRNF 

        70         80         90        100        110        120 
VLTAAHCAGR SITVLLGAHN KTSKEDTWQK LEVEKQFLHP KYDENLVVHD IMLLKLKEKA 

       130        140        150        160        170        180 
KLTLGVGTLP LSANFNFIPP GRMCRAVGWG RTNVNEPASD TLQEVKMRLQ EPQACKHFTS 

       190        200        210        220        230        240 
FRHNSQLCVG NPKKMQNVYK GDSGGPLLCA GIAQGIASYV HRNAKPPAVF TRISHYRPWI 


NKILREN 

P21844 in FASTA format

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