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UniProtKB/Swiss-Prot entry P21730


[Entry info] [Name and origin] [References] [Comments] [Cross-references] [Keywords] [Features] [Sequence] [Tools]

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Entry information
Entry name C5AR_HUMAN
Primary accession number P21730
Secondary accession numbers None
Integrated into Swiss-Prot on May 1, 1991
Sequence was last modified on May 1, 1991 (Sequence version 1)
Annotations were last modified on    November 4, 2008 (Entry version 88)
Name and origin of the protein
Protein name C5a anaphylatoxin chemotactic receptor
Synonyms C5a-R
C5aR
CD88 antigen
Gene name
Name: C5AR1
Synonyms: C5AR, C5R1
From
Homo sapiens (Human) [TaxID: 9606] 
Taxonomy Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini; Catarrhini; Hominidae; Homo.
Protein existence 1: Evidence at protein level;
References
[1]
NUCLEOTIDE SEQUENCE [GENOMIC DNA / MRNA].
DOI=10.1038/349614a0; PubMed=1847994 [NCBI, ExPASy, EBI, Israel, Japan]
Gerard N.P., Gerard C.;
"The chemotactic receptor for human C5a anaphylatoxin.";
Nature 349:614-617(1991).
[2]
NUCLEOTIDE SEQUENCE [MRNA].
DOI=10.1021/bi00226a002; PubMed=2007135 [NCBI, ExPASy, EBI, Israel, Japan]
Boulay F., Mery L., Tardif M., Brouchon L., Vignais P.;
"Expression cloning of a receptor for C5a anaphylatoxin on differentiated HL-60 cells.";
Biochemistry 30:2993-2999(1991).
[3]
NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
Kopatz S.A., Aronstam R.S., Sharma S.V.;
"cDNA clones of human proteins involved in signal transduction sequenced by the Guthrie cDNA resource center (www.cdna.org).";
Submitted (JAN-2003) to the EMBL/GenBank/DDBJ databases.
[4]
NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
Kalnine N., Chen X., Rolfs A., Halleck A., Hines L., Eisenstein S., Koundinya M., Raphael J., Moreira D., Kelley T., LaBaer J., Lin Y., Phelan M., Farmer A.;
"Cloning of human full-length CDSs in BD Creator(TM) system donor vector.";
Submitted (MAY-2003) to the EMBL/GenBank/DDBJ databases.
[5]
NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
TISSUE=Brain;
DOI=10.1101/gr.2596504; PubMed=15489334 [NCBI, ExPASy, EBI, Israel, Japan]
The MGC Project Team;
"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC).";
Genome Res. 14:2121-2127(2004).
[6]
NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 1-3.
DOI=10.1021/bi00056a007; PubMed=8383526 [NCBI, ExPASy, EBI, Israel, Japan]
Gerard N.P., Bao L., Xiao-Ping H., Eddy R.L. Jr., Shows T.B., Gerard C.;
"Human chemotaxis receptor genes cluster at 19q13.3-13.4. Characterization of the human C5a receptor gene.";
Biochemistry 32:1243-1250(1993).
[7]
PHOSPHORYLATION AT SER-314; SER-317; SER-327; SER-332; SER-334 AND SER-338.
DOI=10.1074/jbc.270.32.19166; PubMed=7642584 [NCBI, ExPASy, EBI, Israel, Japan]
Giannini E., Brouchon L., Boulay F.;
"Identification of the major phosphorylation sites in human C5a anaphylatoxin receptor in vivo.";
J. Biol. Chem. 270:19166-19172(1995).
[8]
SULFATION AT TYR-11 AND TYR-14.
DOI=10.1084/jem.193.9.1059; PubMed=11342590 [NCBI, ExPASy, EBI, Israel, Japan]
Farzan M., Schnitzler C.E., Vasilieva N., Leung D., Kuhn J., Gerard C., Gerard N.P., Choe H.;
"Sulfated tyrosines contribute to the formation of the c5a docking site of the human c5a anaphylatoxin receptor.";
J. Exp. Med. 193:1059-1066(2001).
Comments
  • FUNCTION: Receptor for the chemotactic and inflammatory peptide anaphylatoxin C5a. This receptor stimulates chemotaxis, granule enzyme release and superoxide anion production.
  • SUBCELLULAR LOCATION: Cell membrane; Multi-pass membrane protein.
  • PTM: Sulfation plays a critical role in the association of the receptor with C5a, but no significant role in the ability of the receptor to transduce a signal and mobilize calcium in response to a small peptide agonist.
  • SIMILARITY: Belongs to the G-protein coupled receptor 1 family [view classification].
Copyright
Copyrighted by the UniProt Consortium, see http://www.uniprot.org/terms. Distributed under the Creative Commons Attribution-NoDerivs License.
Cross-references
Sequence databases
EMBL
X57250; CAA40530.1; -; mRNA.[EMBL / GenBank / DDBJ] [CoDingSequence]
X58674; CAB37830.1; -; Genomic_DNA.[EMBL / GenBank / DDBJ] [CoDingSequence]
M62505; AAA62831.1; -; mRNA.[EMBL / GenBank / DDBJ] [CoDingSequence]
AY221091; AAO65969.1; -; Genomic_DNA.[EMBL / GenBank / DDBJ] [CoDingSequence]
BT007358; AAP36022.1; -; mRNA.[EMBL / GenBank / DDBJ] [CoDingSequence]
BC008982; AAH08982.1; -; mRNA.[EMBL / GenBank / DDBJ] [CoDingSequence]
S56556; AAD14919.1; -; Genomic_DNA.[EMBL / GenBank / DDBJ] [CoDingSequence]
S56557; AAD14919.1; JOINED; Genomic_DNA.[EMBL / GenBank / DDBJ] [CoDingSequence]
PIR A37963; A37963.
RefSeq NP_001727.1; -.
UniGene Hs.2161
3D structure databases
ModBase P21730.
Protein family/group databases
GPCRDB P21730; C5AR_HUMAN.
PTM databases
PhosphoSite P21730; -.
Organism-specific databases
H-InvDB HIX0015270; -.
HGNC HGNC:1338; C5AR1.
GenAtlas C5AR1.
HPA CAB002720; -.
HPA014520; -.
MIM 113995; gene. [NCBI / EBI]
Orphanet 101994; Complement regulatory proteins anomaly.
GeneCards P21730.
Gene expression databases
ArrayExpress P21730; -.
CleanEx HS_C5AR1; -.
GermOnline ENSG00000197405; Homo sapiens.
Ontologies
GO
GO:0005887; Cellular component: integral to plasma membrane (traceable author statement from ProtInc).
GO:0004944; Molecular function: C5a anaphylatoxin receptor activity (traceable author statement from ProtInc).
GO:0000187; Biological process: activation of MAPK activity (traceable author statement from ProtInc).
GO:0007202; Biological process: activation of phospholipase C activity (traceable author statement from ProtInc).
GO:0006968; Biological process: cellular defense response (traceable author statement from ProtInc).
GO:0006935; Biological process: chemotaxis (traceable author statement from ProtInc).
GO:0007204; Biological process: elevation of cytosolic calcium ion concentration (traceable author statement from ProtInc).
GO:0006955; Biological process: immune response (traceable author statement from ProtInc).
GO:0007606; Biological process: sensory perception of chemical stimulus (traceable author statement from ProtInc).
QuickGo view.
Family and domain databases
InterPro IPR002234; Anphylx_rcpt.
IPR001274; C5A_anaphtx_rcpt.
IPR000276; GPCR_Rhodpsn.
IPR017452; GPCR_Rhodpsn_supfam.
Graphical view of domain structure.
Pfam PF00001; 7tm_1; 1.
Pfam graphical view of domain structure.
PRINTS PR01104; ANPHYLATOXNR.
PR00426; C5ANPHYLTXNR.
PR00237; GPCRRHODOPSN.
PROSITE PS00237; G_PROTEIN_RECEP_F1_1; 1.
PS50262; G_PROTEIN_RECEP_F1_2; 1.
PROSITE graphical view of domain structure (profiles).
ProtoNet P21730.
Genome annotation databases
Ensembl ENSG00000197405; Homo sapiens. [Contig view]
GeneID 728; -.
KEGG hsa:728; -.
Phylogenomic databases
HOGENOM P21730; -.
HOVERGEN P21730; -.
Other
NextBio 2964; -.
SOURCE C5AR1; Homo sapiens.
GPCRDB-Snakes P21730.
UniRef View cluster of proteins with at least 50% / 90% / 100% identity.
Keywords
Cell membrane; Chemotaxis; G-protein coupled receptor; Glycoprotein; Membrane; Phosphoprotein; Receptor; Sulfation; Transducer; Transmembrane.
Features
SEVIEWER logo Feature table viewer FT aligner logo Feature aligner
KeyFrom   To Length Description FTId
CHAIN   1   350  350     C5a anaphylatoxin chemotactic receptor. PRO_0000069209
TOPO_DOM   1    37  37     Extracellular (Potential). 
TRANSMEM   38    60  23     1 (Potential). 
TOPO_DOM   61    71  11     Cytoplasmic (Potential). 
TRANSMEM   72    94  23     2 (Potential). 
TOPO_DOM   95   110  16     Extracellular (Potential). 
TRANSMEM   111   132  22     3 (Potential). 
TOPO_DOM   133   153  21     Cytoplasmic (Potential). 
TRANSMEM   154   174  21     4 (Potential). 
TOPO_DOM   175   200  26     Extracellular (Potential). 
TRANSMEM   201   226  26     5 (Potential). 
TOPO_DOM   227   242  16     Cytoplasmic (Potential). 
TRANSMEM   243   265  23     6 (Potential). 
TOPO_DOM   266   282  17     Extracellular (Potential). 
TRANSMEM   283   303  21     7 (Potential). 
TOPO_DOM   304   350  47     Cytoplasmic (Potential). 
MOD_RES   11    11        Sulfotyrosine. 
MOD_RES   14    14        Sulfotyrosine. 
MOD_RES   314   314        Phosphoserine (Probable). 
MOD_RES   317   317        Phosphoserine (Probable). 
MOD_RES   327   327        Phosphoserine (Probable). 
MOD_RES   332   332        Phosphoserine (Probable). 
MOD_RES   334   334        Phosphoserine (Probable). 
MOD_RES   338   338        Phosphoserine (Probable). 
CARBOHYD   5     5        N-linked (GlcNAc...) (Potential). 
DISULFID   109   188        By similarity. 
Sequence information
Length: 350 AA [This is the length of the unprocessed precursor] Molecular weight: 39321 Da [This is the MW of the unprocessed precursor] CRC64: DD1D0D71D327CDD0 [This is a checksum on the sequence]
        10         20         30         40         50         60 
MNSFNYTTPD YGHYDDKDTL DLNTPVDKTS NTLRVPDILA LVIFAVVFLV GVLGNALVVW 

        70         80         90        100        110        120 
VTAFEAKRTI NAIWFLNLAV ADFLSCLALP ILFTSIVQHH HWPFGGAACS ILPSLILLNM 

       130        140        150        160        170        180 
YASILLLATI SADRFLLVFK PIWCQNFRGA GLAWIACAVA WGLALLLTIP SFLYRVVREE 

       190        200        210        220        230        240 
YFPPKVLCGV DYSHDKRRER AVAIVRLVLG FLWPLLTLTI CYTFILLRTW SRRATRSTKT 

       250        260        270        280        290        300 
LKVVVAVVAS FFIFWLPYQV TGIMMSFLEP SSPTFLLLNK LDSLCVSFAY INCCINPIIY 

       310        320        330        340        350 
VVAGQGFQGR LRKSLPSLLR NVLTEESVVR ESKSFTRSTV DTMAQKTQAV 

P21730 in FASTA format

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View entry in raw text format (no links)
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