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UniProtKB/Swiss-Prot entry P21589


[Entry info] [Name and origin] [References] [Comments] [Cross-references] [Keywords] [Features] [Sequence] [Tools]

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Entry information
Entry name 5NTD_HUMAN
Primary accession number P21589
Secondary accession numbers O75520 Q5W116
Integrated into Swiss-Prot on May 1, 1991
Sequence was last modified on May 1, 1991 (Sequence version 1)
Annotations were last modified on    November 25, 2008 (Entry version 92)
Name and origin of the protein
Protein name 5'-nucleotidase [Precursor]
Synonyms EC 3.1.3.5
Ecto-5'-nucleotidase
5'-NT
CD73 antigen
Gene name
Name: NT5E
Synonyms: NT5, NTE
From
Homo sapiens (Human) [TaxID: 9606] 
Taxonomy Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini; Catarrhini; Hominidae; Homo.
Protein existence 1: Evidence at protein level;
References
[1]
NUCLEOTIDE SEQUENCE [MRNA], PARTIAL PROTEIN SEQUENCE, AND GPI-ANCHOR AT SER-549.
TISSUE=Placenta;
DOI=10.1111/j.1432-1033.1990.tb19158.x; PubMed=2129526 [NCBI, ExPASy, EBI, Israel, Japan]
Misumi Y., Ogata S., Ohkubo K., Hirose S., Ikehara Y.;
"Primary structure of human placental 5'-nucleotidase and identification of the glycolipid anchor in the mature form.";
Eur. J. Biochem. 191:563-569(1990).
[2]
NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
DOI=10.1038/nature02055; PubMed=14574404 [NCBI, ExPASy, EBI, Israel, Japan]
Mungall A.J., Palmer S.A., Sims S.K., Edwards C.A., Ashurst J.L., Wilming L., Jones M.C., Horton R., Hunt S.E., Scott C.E., Gilbert J.G.R., Clamp M.E., Bethel G., Milne S., Ainscough R., Almeida J.P., Ambrose K.D., Andrews T.D., Ashwell R.I.S., Babbage A.K., Bagguley C.L., Bailey J., Banerjee R., Barker D.J., Barlow K.F., Bates K., Beare D.M., Beasley H., Beasley O., Bird C.P., Blakey S.E., Bray-Allen S., Brook J., Brown A.J., Brown J.Y., Burford D.C., Burrill W., Burton J., Carder C., Carter N.P., Chapman J.C., Clark S.Y., Clark G., Clee C.M., Clegg S., Cobley V., Collier R.E., Collins J.E., Colman L.K., Corby N.R., Coville G.J., Culley K.M., Dhami P., Davies J., Dunn M., Earthrowl M.E., Ellington A.E., Evans K.A., Faulkner L., Francis M.D., Frankish A., Frankland J., French L., Garner P., Garnett J., Ghori M.J., Gilby L.M., Gillson C.J., Glithero R.J., Grafham D.V., Grant M., Gribble S., Griffiths C., Griffiths M.N.D., Hall R., Halls K.S., Hammond S., Harley J.L., Hart E.A., Heath P.D., Heathcott R., Holmes S.J., Howden P.J., Howe K.L., Howell G.R., Huckle E., Humphray S.J., Humphries M.D., Hunt A.R., Johnson C.M., Joy A.A., Kay M., Keenan S.J., Kimberley A.M., King A., Laird G.K., Langford C., Lawlor S., Leongamornlert D.A., Leversha M., Lloyd C.R., Lloyd D.M., Loveland J.E., Lovell J., Martin S., Mashreghi-Mohammadi M., Maslen G.L., Matthews L., McCann O.T., McLaren S.J., McLay K., McMurray A., Moore M.J.F., Mullikin J.C., Niblett D., Nickerson T., Novik K.L., Oliver K., Overton-Larty E.K., Parker A., Patel R., Pearce A.V., Peck A.I., Phillimore B.J.C.T., Phillips S., Plumb R.W., Porter K.M., Ramsey Y., Ranby S.A., Rice C.M., Ross M.T., Searle S.M., Sehra H.K., Sheridan E., Skuce C.D., Smith S., Smith M., Spraggon L., Squares S.L., Steward C.A., Sycamore N., Tamlyn-Hall G., Tester J., Theaker A.J., Thomas D.W., Thorpe A., Tracey A., Tromans A., Tubby B., Wall M., Wallis J.M., West A.P., White S.S., Whitehead S.L., Whittaker H., Wild A., Willey D.J., Wilmer T.E., Wood J.M., Wray P.W., Wyatt J.C., Young L., Younger R.M., Bentley D.R., Coulson A., Durbin R.M., Hubbard T., Sulston J.E., Dunham I., Rogers J., Beck S.;
"The DNA sequence and analysis of human chromosome 6.";
Nature 425:805-811(2003).
[3]
NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 1-113.
TISSUE=Placenta;
DOI=10.1016/0378-1119(95)00574-9; PubMed=8566797 [NCBI, ExPASy, EBI, Israel, Japan]
Hansen K.R., Resta R., Webb C.F., Thompson L.F.;
"Isolation and characterization of the promoter of the human 5'-nucleotidase (CD73)-encoding gene.";
Gene 167:307-312(1995).
[4]
NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 359-489, AND VARIANT ALA-376.
TISSUE=Leukocyte;
Zanoni L., Rosi F., Pagani R., Marinello E.;
Submitted (MAY-1998) to the EMBL/GenBank/DDBJ databases.
[5]
PROTEIN SEQUENCE OF 27-40.
TISSUE=Placenta;
DOI=10.1016/0006-291X(90)91601-N; PubMed=2173922 [NCBI, ExPASy, EBI, Israel, Japan]
Klemens M.R., Sherman W.R., Holmberg N.J., Ruedi J.M., Low M.G., Thompson L.F.;
"Characterization of soluble vs membrane-bound human placental 5'-nucleotidase.";
Biochem. Biophys. Res. Commun. 172:1371-1377(1990).
[6]
DISEASE.
DOI=10.1016/S0024-3205(98)00206-9; PubMed=9651114 [NCBI, ExPASy, EBI, Israel, Japan]
Rosi F., Agostinho A.B., Carlucci F., Zanoni L., Porcelli B., Marinello E., Galieni P., Tabucchi A.;
"Behaviour of human lymphocytic isoenzymes of 5'-nucleotidase.";
Life Sci. 62:2257-2266(1998).
Comments
Copyright
Copyrighted by the UniProt Consortium, see http://www.uniprot.org/terms. Distributed under the Creative Commons Attribution-NoDerivs License.
Cross-references
Sequence databases
EMBL
X55740; CAA39271.1; -; mRNA.[EMBL / GenBank / DDBJ] [CoDingSequence]
AL135903; CAH72337.1; -; Genomic_DNA.[EMBL / GenBank / DDBJ] [CoDingSequence]
AL589666; CAH72337.1; JOINED; Genomic_DNA.[EMBL / GenBank / DDBJ] [CoDingSequence]
AL589666; CAI40168.1; -; Genomic_DNA.[EMBL / GenBank / DDBJ] [CoDingSequence]
AL135903; CAI40168.1; JOINED; Genomic_DNA.[EMBL / GenBank / DDBJ] [CoDingSequence]
U21730; AAA96950.1; -; Genomic_DNA.[EMBL / GenBank / DDBJ] [CoDingSequence]
AF069067; AAC98672.1; -; Genomic_DNA.[EMBL / GenBank / DDBJ] [CoDingSequence]
PIR S11032; S11032.
RefSeq NP_002517.1; -.
UniGene Hs.153952
3D structure databases
HSSP P07024; 2USH. [HSSP ENTRY / PDB]
ModBase P21589.
PTM databases
PhosphoSite P21589; -.
Enzyme and pathway databases
Reactome REACT_1698; Nucleotide metabolism.
Organism-specific databases
H-InvDB HIX0032840; -.
HGNC HGNC:8021; NT5E.
GenAtlas NT5E.
HPA HPA017357; -.
MIM 129190; gene. [NCBI / EBI]
PharmGKB PA31804; -.
GeneCards P21589.
Gene expression databases
ArrayExpress P21589; -.
CleanEx HS_NT5E; -.
GermOnline ENSG00000135318; Homo sapiens.
Ontologies
GO
GO:0031225; Cellular component: anchored to membrane (inferred from electronic annotation from UniProtKB-KW).
GO:0005624; Cellular component: membrane fraction (traceable author statement from ProtInc).
GO:0005886; Cellular component: plasma membrane (inferred from electronic annotation from UniProtKB-KW).
GO:0008253; Molecular function: 5'-nucleotidase activity (traceable author statement from ProtInc).
GO:0000166; Molecular function: nucleotide binding (inferred from electronic annotation from InterPro).
GO:0008270; Molecular function: zinc ion binding (inferred from electronic annotation from UniProtKB-KW).
GO:0006259; Biological process: DNA metabolic process (traceable author statement from ProtInc).
GO:0009166; Biological process: nucleotide catabolic process (inferred from electronic annotation from InterPro).
QuickGo view.
Family and domain databases
InterPro IPR008334; 5'-Nucleotdase_C.
IPR006146; 5'-Nucleotdase_CS.
IPR006179; 5_nucleotidase.
IPR004843; M-pesterase.
Graphical view of domain structure.
Gene3D G3DSA:3.90.780.10; 5'-Nucleotdase_C; 1.
PANTHER PTHR11575; 5_nucleotidase; 1.
Pfam PF02872; 5_nucleotid_C; 1.
PF00149; Metallophos; 1.
Pfam graphical view of domain structure.
PRINTS PR01607; APYRASEFAMLY.
PROSITE PS00785; 5_NUCLEOTIDASE_1; 1.
PS00786; 5_NUCLEOTIDASE_2; 1.
ProtoNet P21589.
Genome annotation databases
Ensembl ENSG00000135318; Homo sapiens. [Contig view]
GeneID 4907; -.
KEGG hsa:4907; -.
Phylogenomic databases
HOGENOM P21589; -.
HOVERGEN P21589; -.
Other
DrugBank DB00806; Pentoxifylline.
NextBio 18883; -.
SOURCE NT5E; Homo sapiens.
UniRef View cluster of proteins with at least 50% / 90% / 100% identity.
Keywords
Cell membrane; Direct protein sequencing; Glycoprotein; GPI-anchor; Hydrolase; Lipoprotein; Membrane; Metal-binding; Nucleotide-binding; Polymorphism; Signal; Zinc.
Features
SEVIEWER logo Feature table viewer FT aligner logo Feature aligner
KeyFrom   To Length Description FTId
SIGNAL   1    26  26      
CHAIN   27   549  523     5'-nucleotidase. PRO_0000000015
PROPEP   550   574  25     Removed in mature form. PRO_0000000016
REGION   500   506  7     Substrate binding (By similarity). 
METAL   36    36        Zinc 1 (By similarity). 
METAL   38    38        Zinc 1 (By similarity). 
METAL   85    85        Zinc 1 (By similarity). 
METAL   85    85        Zinc 2 (By similarity). 
METAL   117   117        Zinc 2 (By similarity). 
METAL   220   220        Zinc 2 (By similarity). 
METAL   243   243        Zinc 2 (By similarity). 
BINDING   417   417        Substrate (By similarity). 
SITE   118   118  1     Transition state stabilizer (By similarity). 
SITE   121   121  1     Transition state stabilizer (By similarity). 
LIPID   549   549        GPI-anchor amidated serine. 
CARBOHYD   53    53        N-linked (GlcNAc...) (Potential). 
CARBOHYD   311   311        N-linked (GlcNAc...) (Potential). 
CARBOHYD   333   333        N-linked (GlcNAc...) (Potential). 
CARBOHYD   403   403        N-linked (GlcNAc...) (Potential). 
VARIANT   376   376  1     T -> A (in dbSNP:rs3812138 [NCBI]). VAR_022091 
Sequence information
Length: 574 AA [This is the length of the unprocessed precursor] Molecular weight: 63368 Da [This is the MW of the unprocessed precursor] CRC64: A99AF170AB7EAECE [This is a checksum on the sequence]
        10         20         30         40         50         60 
MCPRAARAPA TLLLALGAVL WPAAGAWELT ILHTNDVHSR LEQTSEDSSK CVNASRCMGG 

        70         80         90        100        110        120 
VARLFTKVQQ IRRAEPNVLL LDAGDQYQGT IWFTVYKGAE VAHFMNALRY DAMALGNHEF 

       130        140        150        160        170        180 
DNGVEGLIEP LLKEAKFPIL SANIKAKGPL ASQISGLYLP YKVLPVGDEV VGIVGYTSKE 

       190        200        210        220        230        240 
TPFLSNPGTN LVFEDEITAL QPEVDKLKTL NVNKIIALGH SGFEMDKLIA QKVRGVDVVV 

       250        260        270        280        290        300 
GGHSNTFLYT GNPPSKEVPA GKYPFIVTSD DGRKVPVVQA YAFGKYLGYL KIEFDERGNV 

       310        320        330        340        350        360 
ISSHGNPILL NSSIPEDPSI KADINKWRIK LDNYSTQELG KTIVYLDGSS QSCRFRECNM 

       370        380        390        400        410        420 
GNLICDAMIN NNLRHTDEMF WNHVSMCILN GGGIRSPIDE RNNGTITWEN LAAVLPFGGT 

       430        440        450        460        470        480 
FDLVQLKGST LKKAFEHSVH RYGQSTGEFL QVGGIHVVYD LSRKPGDRVV KLDVLCTKCR 

       490        500        510        520        530        540 
VPSYDPLKMD EVYKVILPNF LANGGDGFQM IKDELLRHDS GDQDINVVST YISKMKVIYP 

       550        560        570 
AVEGRIKFST GSHCHGSFSL IFLSLWAVIF VLYQ 

P21589 in FASTA format

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