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UniProtKB/Swiss-Prot entry P21588

[Entry info] [Name and origin] [References] [Comments] [Cross-references] [Keywords] [Features] [Sequence] [Tools]

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Entry information
Entry name 5NTD_RAT
Primary accession number P21588
Secondary accession numbers None
Integrated into Swiss-Prot on May 1, 1991
Sequence was last modified on May 1, 1991 (Sequence version 1)
Annotations were last modified on    November 25, 2008 (Entry version 79)
Name and origin of the protein
Protein name 5'-nucleotidase [Precursor]
Synonyms EC 3.1.3.5
Ecto-5'-nucleotidase
5'-NT
CD73 antigen
Gene name
Name: Nt5e
Synonyms: Nt5, Nte
From
Rattus norvegicus (Rat) [TaxID: 10116] 
Taxonomy Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Sciurognathi; Muroidea; Muridae; Murinae; Rattus.
Protein existence 1: Evidence at protein level;
References
[1]
 NUCLEOTIDE SEQUENCE [MRNA], AND PARTIAL PROTEIN SEQUENCE.
TISSUE=Liver;
PubMed=2298743 [NCBI, ExPASy, EBI, Israel, Japan]
Misumi Y., Ogata S., Hirose S., Ikehara Y.;
"Primary structure of rat liver 5'-nucleotidase deduced from the cDNA. Presence of the COOH-terminal hydrophobic domain for possible post-translational modification by glycophospholipid.";
J. Biol. Chem. 265:2178-2183(1990).
[2]
 PROTEIN SEQUENCE OF 538-551, AND GPI-ANCHOR AT SER-551.
TISSUE=Liver;
DOI=10.1021/bi00486a021; PubMed=2148114 [NCBI, ExPASy, EBI, Israel, Japan]
Ogata S., Hayashi Y., Misumi Y., Ikehara Y.;
"Membrane-anchoring domain of rat liver 5'-nucleotidase: identification of the COOH-terminal serine-523 covalently attached with a glycolipid.";
Biochemistry 29:7923-7927(1990).
Comments
Copyright
Copyrighted by the UniProt Consortium, see http://www.uniprot.org/terms. Distributed under the Creative Commons Attribution-NoDerivs License.
Cross-references
Sequence databases
EMBL
J05214; AAA40621.1; -; mRNA.[EMBL / GenBank / DDBJ] [CoDingSequence]
PIR A35036; A35036.
UniGene Rn.40132
3D structure databases
HSSP P07024; 2USH. [HSSP ENTRY / PDB]
ModBase P21588.
Organism-specific databases
RGD 61956; Nt5e.
Gene expression databases
ArrayExpress P21588; -.
GermOnline ENSRNOG00000011071; Rattus norvegicus.
Ontologies
GO
GO:0031225; Cellular component: anchored to membrane (inferred from electronic annotation from UniProtKB-KW).
GO:0005886; Cellular component: plasma membrane (inferred from electronic annotation from UniProtKB-KW).
GO:0008253; Molecular function: 5'-nucleotidase activity (inferred from electronic annotation from EC).
GO:0000166; Molecular function: nucleotide binding (inferred from electronic annotation from InterPro).
GO:0008270; Molecular function: zinc ion binding (inferred from electronic annotation from UniProtKB-KW).
GO:0009166; Biological process: nucleotide catabolic process (inferred from electronic annotation from InterPro).
QuickGo view.
Family and domain databases
InterPro IPR008334; 5'-Nucleotdase_C.
IPR006146; 5'-Nucleotdase_CS.
IPR006179; 5_nucleotidase.
IPR004843; M-pesterase.
Graphical view of domain structure.
Gene3D G3DSA:3.90.780.10; 5'-Nucleotdase_C; 1.
PANTHER PTHR11575; 5_nucleotidase; 1.
Pfam PF02872; 5_nucleotid_C; 1.
PF00149; Metallophos; 1.
Pfam graphical view of domain structure.
PRINTS PR01607; APYRASEFAMLY.
PROSITE PS00785; 5_NUCLEOTIDASE_1; 1.
PS00786; 5_NUCLEOTIDASE_2; 1.
ProtoNet P21588.
Genome annotation databases
Ensembl ENSRNOG00000011071; Rattus norvegicus. [Contig view]
Phylogenomic databases
HOVERGEN P21588; -.
Other
UniRef View cluster of proteins with at least 50% / 90% / 100% identity.
Keywords
Cell membrane; Direct protein sequencing; Glycoprotein; GPI-anchor; Hydrolase; Lipoprotein; Membrane; Metal-binding; Nucleotide-binding; Signal; Zinc.
Features
SEVIEWER logo Feature table viewer FT aligner logo Feature aligner
KeyFrom   To Length Description FTId
SIGNAL   1    28  28      
CHAIN   29   551  523     5'-nucleotidase. PRO_0000000019
PROPEP   552   576  25     Removed in mature form. PRO_0000000020
REGION   502   508  7     Substrate binding (By similarity). 
METAL   38    38        Zinc 1 (By similarity). 
METAL   40    40        Zinc 1 (By similarity). 
METAL   87    87        Zinc 1 (By similarity). 
METAL   87    87        Zinc 2 (By similarity). 
METAL   119   119        Zinc 2 (By similarity). 
METAL   222   222        Zinc 2 (By similarity). 
METAL   245   245        Zinc 2 (By similarity). 
BINDING   419   419        Substrate (By similarity). 
SITE   120   120  1     Transition state stabilizer (By similarity). 
SITE   123   123  1     Transition state stabilizer (By similarity). 
LIPID   551   551        GPI-anchor amidated serine. 
CARBOHYD   55    55        N-linked (GlcNAc...) (Potential). 
CARBOHYD   313   313        N-linked (GlcNAc...) (Potential). 
CARBOHYD   335   335        N-linked (GlcNAc...) (Potential). 
CARBOHYD   349   349        N-linked (GlcNAc...) (Potential). 
CARBOHYD   405   405        N-linked (GlcNAc...) (Potential). 
Sequence information
Length: 576 AA [This is the length of the unprocessed precursor] Molecular weight: 63969 Da [This is the MW of the unprocessed precursor] CRC64: 9EB75DD51E678AA6 [This is a checksum on the sequence] 
        10         20         30         40         50         60 
MRPAAATAPK WLLLALSALL PLWPTAKSWE LTIMHTNDVH SRLEQTSDDS TKCLNASLCV 

        70         80         90        100        110        120 
GGVARLFTKV QQIRKEEPNV LLLDAGDQYQ GTIWFTVYKG LEVAHFMNLL GYDAMALGNH 

       130        140        150        160        170        180 
EFDNGVEGLI DPLLRNVKFP ILSANIKARG PLAPQISGLY LPYKVLSVGG EVVGIVGYTS 

       190        200        210        220        230        240 
KETPFLSNPG TNLVFEDEVT ALQPEVDKLK TLNVNKIIAL GHSGFEMDKL IAQKVRGVDV 

       250        260        270        280        290        300 
VVGGHTNTFL YTGNPPSKEV PAGKYPFIVT SDDGRKVPVV QAYAFGKYLG YLKVEFDDKG 

       310        320        330        340        350        360 
NVVTSYGNPI LLNSTIREDA AIKADINQWR IKLDNYSTQE LGRTIVYLNG SAQECRFREC 

       370        380        390        400        410        420 
NMGNLICDAM INNNLRHPDE MFWNHVSMCI VNGGGIRSPI DERNNGTITW ENLAAVLPFG 

       430        440        450        460        470        480 
GTFDLVQLKG STLKKAFEHS VHRYGQSTGE FLQVGGIHVV YDISRKPWDR VVQLKVLCTK 

       490        500        510        520        530        540 
CRVPIYEPLE MDKVYKVVLP SYLVNGGDGF QMIKDELLKH DSGDQDISVV SEYISKMKVI 

       550        560        570 
YPAVEGRIKF SAASHYQGSF PLIILSFWAV ILVLYQ
P21588 in FASTA format

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