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UniProtKB/Swiss-Prot entry P21269


[Entry info] [Name and origin] [References] [Comments] [Cross-references] [Keywords] [Features] [Sequence] [Tools]

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Entry information
Entry name CCA1_YEAST
Primary accession number P21269
Secondary accession numbers None
Integrated into Swiss-Prot on May 1, 1991
Sequence was last modified on May 1, 1991 (Sequence version 1)
Annotations were last modified on    November 25, 2008 (Entry version 82)
Name and origin of the protein
Protein name tRNA nucleotidyltransferase, mitochondrial [Precursor]
Synonyms EC 2.7.7.25
tRNA adenylyltransferase
tRNA CCA-pyrophosphorylase
CCA-adding enzyme
Gene name
Name: CCA1
Synonyms: TNT1
OrderedLocusNames: YER168C
From
Saccharomyces cerevisiae (Baker's yeast) [TaxID: 4932] 
Taxonomy Eukaryota; Fungi; Dikarya; Ascomycota; Saccharomycotina; Saccharomycetes; Saccharomycetales; Saccharomycetaceae; Saccharomyces.
Protein existence 1: Evidence at protein level;
References
[1]
NUCLEOTIDE SEQUENCE [GENOMIC DNA].
PubMed=2204621 [NCBI, ExPASy, EBI, Israel, Japan]
Aebi M., Kirchner G., Chen J.Y., Vijayraghavan U., Jacobson A., Martin N.C., Abelson J.;
"Isolation of a temperature-sensitive mutant with an altered tRNA nucleotidyltransferase and cloning of the gene encoding tRNA nucleotidyltransferase in the yeast Saccharomyces cerevisiae.";
J. Biol. Chem. 265:16216-16220(1990).
[2]
NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
STRAIN=ATCC 204511 / S288c / AB972;
PubMed=9169868 [NCBI, ExPASy, EBI, Israel, Japan]
Dietrich F.S., Mulligan J.T., Hennessy K.M., Yelton M.A., Allen E., Araujo R., Aviles E., Berno A., Brennan T., Carpenter J., Chen E., Cherry J.M., Chung E., Duncan M., Guzman E., Hartzell G., Hunicke-Smith S., Hyman R.W., Kayser A., Komp C., Lashkari D., Lew H., Lin D., Mosedale D., Nakahara K., Namath A., Norgren R., Oefner P., Oh C., Petel F.X., Roberts D., Sehl P., Schramm S., Shogren T., Smith V., Taylor P., Wei Y., Botstein D., Davis R.W.;
"The nucleotide sequence of Saccharomyces cerevisiae chromosome V.";
Nature 387:78-81(1997).
[3]
ALTERNATIVE INITIATION, AND PROTEIN SEQUENCE OF 19-26.
PubMed=8175766 [NCBI, ExPASy, EBI, Israel, Japan]
Wolfe C.L., Lou Y.-C., Hopper A.K., Martin N.C.;
"Interplay of heterogeneous transcriptional start sites and translational selection of AUGs dictate the production of mitochondrial and cytosolic/nuclear tRNA nucleotidyltransferase from the same gene in yeast.";
J. Biol. Chem. 269:13361-13366(1994).
[4]
LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
DOI=10.1038/nature02046; PubMed=14562106 [NCBI, ExPASy, EBI, Israel, Japan]
Ghaemmaghami S., Huh W.-K., Bower K., Howson R.W., Belle A., Dephoure N., O'Shea E.K., Weissman J.S.;
"Global analysis of protein expression in yeast.";
Nature 425:737-741(2003).
[5]
PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-21, AND MASS SPECTROMETRY.
DOI=10.1074/mcp.M700468-MCP200; PubMed=18407956 [NCBI, ExPASy, EBI, Israel, Japan]
Albuquerque C.P., Smolka M.B., Payne S.H., Bafna V., Eng J., Zhou H.;
"A multidimensional chromatography technology for in-depth phosphoproteome analysis.";
Mol. Cell. Proteomics 7:1389-1396(2008).
Comments
Copyright
Copyrighted by the UniProt Consortium, see http://www.uniprot.org/terms. Distributed under the Creative Commons Attribution-NoDerivs License.
Cross-references
Sequence databases
EMBL
M59870; AAA35160.1; -; Genomic_DNA.[EMBL / GenBank / DDBJ] [CoDingSequence]
U18922; AAB64695.1; -; Genomic_DNA.[EMBL / GenBank / DDBJ] [CoDingSequence]
PIR S11180; S11180.
RefSeq NP_011095.1; -.
3D structure databases
ModBase P21269.
Protein-protein interaction databases
DIP DIP:2841N; -.
IntAct P21269; -.
Organism-specific databases
CYGD YER168c; -.
SGD S000000970; CCA1.
Yeast-GFP YER168C.
Gene expression databases
ArrayExpress P21269; -.
GermOnline YER168C; Saccharomyces cerevisiae.
Ontologies
GO
GO:0005759; Cellular component: mitochondrial matrix (inferred from direct assay from SGD).
GO:0005634; Cellular component: nucleus (inferred from electronic annotation from UniProtKB-KW).
GO:0000166; Molecular function: nucleotide binding (inferred from electronic annotation from UniProtKB-KW).
GO:0003723; Molecular function: RNA binding (inferred from electronic annotation from InterPro).
GO:0004810; Molecular function: tRNA adenylyltransferase activity (inferred from electronic annotation from EC).
GO:0006400; Biological process: tRNA modification (inferred from direct assay from SGD).
QuickGo view.
Family and domain databases
InterPro IPR002646; PolyA_pol_reg.
Graphical view of domain structure.
Pfam PF01743; PolyA_pol; 1.
Pfam graphical view of domain structure.
ProtoNet P21269.
Proteomic databases
PeptideAtlas P21269; -.
Genome annotation databases
Ensembl YER168C; Saccharomyces cerevisiae. [Contig view]
GeneID 856915; -.
GenomeReviews U00092_GR; YER168C.
KEGG sce:YER168C; -.
NMPDR fig|4932.3.peg.2181; -.
Phylogenomic databases
HOGENOM P21269; -.
Other
LinkHub P21269; -.
NextBio 983365; -.
UniRef View cluster of proteins with at least 50% / 90% / 100% identity.
Keywords
Alternative initiation; Complete proteome; Cytoplasm; Direct protein sequencing; Mitochondrion; Nucleotide-binding; Nucleotidyltransferase; Nucleus; Phosphoprotein; RNA-binding; Transferase; Transit peptide.
Features
SEVIEWER logo Feature table viewer
KeyFrom  To Length Description FTId
TRANSIT   1     ?        Mitochondrion (Potential). 
CHAIN   ?   546        tRNA nucleotidyltransferase, mitochondrial. PRO_0000004779
MOD_RES   21    21        Phosphoserine. 
VAR_SEQ   1    17        Missing (in isoform Cytoplasmic+nuclear 2). VSP_018699
VAR_SEQ   1     9        Missing (in isoform Cytoplasmic+nuclear 1). VSP_018698
Sequence information
Length: 546 AA [This is the length of the unprocessed precursor] Molecular weight: 62485 Da [This is the MW of the unprocessed precursor] CRC64: C0B2B918596E19D5 [This is a checksum on the sequence]
        10         20         30         40         50         60 
MLRSTISLLM NSAAQKTMTN SNFVLNAPKI TLTKVEQNIC NLLNDYTDLY NQKYHNKPEP 

        70         80         90        100        110        120 
LTLRITGGWV RDKLLGQGSH DLDIAINVMS GEQFATGLNE YLQQHYAKYG AKPHNIHKID 

       130        140        150        160        170        180 
KNPEKSKHLE TATTKLFGVE VDFVNLRSEK YTELSRIPKV CFGTPEEDAL RRDATLNALF 

       190        200        210        220        230        240 
YNIHKGEVED FTKRGLQDLK DGVLRTPLPA KQTFLDDPLR VLRLIRFASR FNFTIDPEVM 

       250        260        270        280        290        300 
AEMGDPQINV AFNSKISRER VGVEMEKILV GPTPLLALQL IQRAHLENVI FFWHNDSSVV 

       310        320        330        340        350        360 
KFNEENCQDM DKINHVYNDN ILNSHLKSFI ELYPMFLEKL PILREKIGRS PGFQQNFILS 

       370        380        390        400        410        420 
AILSPMANLQ IIGNPKKKIN NLVSVTESIV KEGLKLSKND AAVIAKTVDS ICSYEEILAK 

       430        440        450        460        470        480 
FADRSQLKKS EIGIFLRNFN GEWETAHFAS LSDAFLKIPK LETKKIELLF QNYNEFYSYI 

       490        500        510        520        530        540 
FDNNLNNCHE LKPIVDGKQM AKLLQMKPGP WLGKINNEAI RWQFDNPTGT DQELITHLKA 


ILPKYL 

P21269 in FASTA format

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