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[1]
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NUCLEOTIDE SEQUENCE [GENOMIC RNA].
Eggenberger A.L.,
Beachy R.N.,
Hill J.H.;
"Two genes of soybean mosaic virus are involved in the interaction with the Rsv1 resistance allele of soybean.";
Submitted (DEC-2000) to the EMBL/GenBank/DDBJ databases.
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[2]
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NUCLEOTIDE SEQUENCE [GENOMIC RNA] OF 2764-3066, AND PROTEIN SEQUENCE OF 2845-2859.
PubMed=2661723 [NCBI, ExPASy, EBI, Israel, Japan]
Eggenberger A.L.,
Stark D.M.,
Beachy R.N.;
"The nucleotide sequence of a soybean mosaic virus coat protein-coding region and its expression in Escherichia coli, Agrobacterium tumefaciens and tobacco callus.";
J. Gen. Virol. 70:1853-1860(1989).
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[3]
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REVIEW.
DOI=10.1016/S0168-1702(01)00220-9; PubMed=11226583 [NCBI, ExPASy, EBI, Israel, Japan]
Urcuqui-Inchima S.,
Haenni A.L.,
Bernardi F.;
"Potyvirus proteins: a wealth of functions.";
Virus Res. 74:157-175(2001).
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- FUNCTION: Coat protein is involved in aphid transmission, cell-to-cell and systemis movement, encapsidation of the viral RNA and in the regulation of viral RNA amplification.
- FUNCTION: Nuclear inclusion protein B is a RNA-dependent RNA polymerase that plays an essential role in the virus replication.
- FUNCTION: Helper component proteinase is required for aphid transmission and also has proteolytic activity. Interacts with virions and aphid stylets. Acts as a suppressor of RNA-mediated gene silencing, also known as post-transcriptional gene silencing (PTGS), a mechanism of plant viral defense that limits the accumulation of viral RNAs. May have RNA-binding activity (By similarity).
- FUNCTION: Cytoplasmic inclusion protein has helicase activity. It may be involved in replication (By similarity).
- FUNCTION: Both 6K peptides are indispensable for virus replication (By similarity).
- FUNCTION: Nuclear inclusion protein A has RNA-binding and proteolytic activities.
- CATALYTIC ACTIVITY: Hydrolyzes a Gly-|-Gly bond at its own C-terminus, commonly in the sequence -Tyr-Xaa-Val-Gly-|-Gly, in the processing of the potyviral polyprotein.
- CATALYTIC ACTIVITY: Hydrolyzes glutaminyl bonds, and activity is further restricted by preferences for the amino acids in P6 - P1' that vary with the species of potyvirus, e.g. Glu-Xaa-Xaa-Tyr-Xaa-Gln-|-(Ser or Gly) for the enzyme from tobacco etch virus. The natural substrate is the viral polyprotein, but other proteins and oligopeptides containing the appropriate consensus sequence are also cleaved.
- CATALYTIC ACTIVITY: Nucleoside triphosphate + RNA(n) = diphosphate + RNA(n+1).
- SUBCELLULAR LOCATION: Coat protein: Virion (Potential).
- DOMAIN: The N-terminus of helper component proteinase is involved in interaction with stylets. The central part is involved in interaction with virions and the C-terminus is involved in cell-to cell movement of the virus.
- PTM: VPg is covalently linked to the genomic RNA (By similarity).
- PTM: The viral RNA of potyviruses is expressed as a single polyprotein which undergoes post-translational proteolytic processing by the main proteinase NIa-pro resulting in the production of at least ten individual proteins. The P1 proteinase and the HC-pro cleave only their respective C-termini autocatalytically. 6K1 is essential for proper proteolytic separation of P3 from CI (By similarity).
- SIMILARITY: Belongs to the potyviruses polyprotein family.
- SIMILARITY: Contains 1 helicase ATP-binding domain.
- SIMILARITY: Contains 1 helicase C-terminal domain.
- SIMILARITY: Contains 1 peptidase C4 domain [view classification].
- SIMILARITY: Contains 1 peptidase C6 domain [view classification].
- SIMILARITY: Contains 1 peptidase S30 domain [view classification].
- SIMILARITY: Contains 1 RdRp catalytic domain.
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Copyrighted by the UniProt Consortium, see http://www.uniprot.org/terms.
Distributed under the Creative Commons Attribution-NoDerivs License.
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| Length: 3066 AA [This is the length of the unprocessed precursor] |
Molecular weight: 349846 Da [This is the MW of the unprocessed precursor] |
CRC64: 394149153DD5328F [This is a checksum on the sequence] |
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10 20 30 40 50 60
MATIMIGSMA ISVPNTHVSC ASNSVMPVQA VQMAKQVPSA RGVLYTLKRE GSTQVHKHEE
70 80 90 100 110 120
ALRKFQEAFD QDVGIQRRLL VNKHSSIQST KKNGLTLRRL TLEQARAKEA AIARRKQEEE
130 140 150 160 170 180
DFLNGKYEQQ FYAGVSATKS MKFEGGSVGF RTKYWRPTPK KTKERRATSQ CRKPTYVLEE
190 200 210 220 230 240
VLSIASKSGK LVEFITGKGK RVKVCYVRKH GAILPKFSLP HEEGKYIHQE LQYASTYEFL
250 260 270 280 290 300
PYICMFAKYK SINADDITYG DSGLLFDERS SLTTNHTKLP YFVVRGRRNG KLVNALEVVE
310 320 330 340 350 360
NMEDIQHYSQ NPEAQFFRGW KKVFDKMPPH VENHECTTDF TNEQCGELAA AISQSIFPVK
370 380 390 400 410 420
KLSCKQCRQH IKHLSWEEYK QFLLAHMGCH GPEWETFQEI DGMRYVKRVI ETSTAENASL
430 440 450 460 470 480
QTSLEIVRLT QNYKSTHMLQ IQDINKALMK GPSVTQSELE QASKQLLAMT QWWKNHMTLT
490 500 510 520 530 540
DEDALKVFRN KRSSKALLNP SLLCDNQLDK NGNFVWGERG RHSKRFFANY FEEVVPSEGY
550 560 570 580 590 600
SKYVIRKNPN GQRELAIGSL IVPLDFERAR MALQGKSVTR EPITMSCISR QDGNFVYPCC
610 620 630 640 650 660
CVTHDDGKAF YSELRSPTKR HLVIGTSGDP KYIDLPATDA DRMYIAKEGF CYLNIFLAML
670 680 690 700 710 720
VNVNEDEAKD FTKMVRDVIV PRLGKWPTML DVATAAYMLT VFHPETRNAE LPRILVDHAC
730 740 750 760 770 780
QTMHVIDSFG SLTVGYHVLK AGTVNQLIQF ASNDLQSEMK FYRVGGEVQQ RMKCETALIT
790 800 810 820 830 840
SIFKPKRMIQ ILENDPYILL MGLVSPSILI HMYRMKHFEK GVELWISKEH SVAKIFIILE
850 860 870 880 890 900
QLTKRVAAND VLLEQLEMIS ETSERFMSIL EDCPQAPHSY KTAKDLLTMY IERKASNNQL
910 920 930 940 950 960
VENGFVDMND KLYMAYEKIY SDRLKQEWRA LSWLEKFSIT WQLKRFAPHT EKCLTKKVVE
970 980 990 1000 1010 1020
ESSASSGNFA SVCFMNAQSH LRNVRNTLFQ KCDQVWTASV RAFVKLIIST LHRCYSDIVY
1030 1040 1050 1060 1070 1080
LVNICIIFSL LVQMTSVLQG IVNTVRRDKA LLSGWKRKED EEAVIHLYEM CEKMEGGHPS
1090 1100 1110 1120 1130 1140
IEKFLDHVKG VRPDLLPVAV SMTGQSEDVS AQAKTATQLQ LEKIVAFMAL LTMCIDNERS
1150 1160 1170 1180 1190 1200
DAVFKVLSKL KAFFSTMGED VKVQSLDEIQ SIDEDKKLTI DFDLETNKES SSVSFDVKFE
1210 1220 1230 1240 1250 1260
AWWNRQLEQN RVIPHYRSTG EFLEFTRETA AKIANLVATS SHTEFLIRGA VGSGKSTGLP
1270 1280 1290 1300 1310 1320
HHLSKKGKVL LLEPTRPLAE NVSKQLSFEP FYHNVTLRMR GMSKFGSSNI VVMTSGFAFH
1330 1340 1350 1360 1370 1380
YYVNNPQQLS DFDFIIIDEC HVQDSPTIAF NCALKEFEFS GKLIKVSATP PGRECEFTTQ
1390 1400 1410 1420 1430 1440
HPVKLKVEDH LSFQNFVQAQ GTGSNADMIQ HGNNLLVYVA SYNEVDQLSR LLTEKHYKVT
1450 1460 1470 1480 1490 1500
KVDGRTMQMG NVEIATTGTE GKPHFIVATN IIENGVTLDI DCVIDFGLKV VATLDTDNRC
1510 1520 1530 1540 1550 1560
VRYNKQSVSY GERIQRLGRV GRCKPGFALR IGHTGKGVEE VPEFIATEAA FLSFAYGLPV
1570 1580 1590 1600 1610 1620
TTQSVSTNIL SRCTVKQARV ALNFELTPFF TTNFIKYDGS MHPEIHRLLK SYKLRESEML
1630 1640 1650 1660 1670 1680
LTKIAIPYQF VGQWVTVKEY ERQGIHLNCP EKVKIPFYVH GIPDKLYEML WDTVCKYKND
1690 1700 1710 1720 1730 1740
AGFGSVKSVN ATKISYTLST DPTAIPRTLA ILDHLLSEEM TKKSHFDTIG SAVTGYSFSL
1750 1760 1770 1780 1790 1800
AGIADGFRKR YLKDYTQHNI AVLQQAKAQL LEFDCNKVDI NNLHNVEGIG ILNAVQLQSK
1810 1820 1830 1840 1850 1860
HEVSKFLQLK GKWDGKKFMN DAVVAIFTLV GGGWMLWDYF TRVIREPVST QGKKRQIQKL
1870 1880 1890 1900 1910 1920
KFRDAFDRKI GREVYADDYT MEHTFGEAYT KKGKQKGSTR TKGMGRKSRN FIHLYGVEPE
1930 1940 1950 1960 1970 1980
NYSMIRFVDP LTGHTMDEHP RVDIRMVQQE FEEIRKDMIG EGELDRQRVY HNPGLQAYFI
1990 2000 2010 2020 2030 2040
GKNTEEALKV DLTPHRPTLL CQNSNAIAGF PEREDELRQT GLPQVVSKSD VPRAKERVEM
2050 2060 2070 2080 2090 2100
ESKSVYKGLR DYSGISTLIC QLTNSSDGHK ETMFGVGYGS FIITNGHLFR RNNGMLTVKT
2110 2120 2130 2140 2150 2160
WHGEFVIHNT TQLKIHFIQG KDVILIRMPK DFPPFGKRNL FRQPKREERV CMVGTNFQEK
2170 2180 2190 2200 2210 2220
SLRATVSESS MILPEGKGSF WIHWITTQDG FCGLPLVSVN DGHIVGIHGL TSNDSEKNFF
2230 2240 2250 2260 2270 2280
VPLTDGFEKE YLENADNLSW DKHWFWEPSK IAWGSLNLVE EQPKEEFKIS KLVSDLFGNT
2290 2300 2310 2320 2330 2340
VTVQGRKERW VLDAMEGNLA ACGQADSALV TKHVVKGKCP YFAQYLSVNQ EAKSFFEPLM
2350 2360 2370 2380 2390 2400
GAYQPSRLNK DAFKRDFFKY NKPVVLNEVD FQSFERAVAG VKLMMMEFDF KECVYVTDPD
2410 2420 2430 2440 2450 2460
EIYDSLNMKA AVGAQYKGKK QDYFSGMDSF DKERLLYLSC ERLFYGEKGV WNGSLKAELR
2470 2480 2490 2500 2510 2520
PIEKVQANKT RTFTAAPIDT LLGAKVCVDD FNNQFYSLNL TCPWTVGMTK FYRGWDKLMR
2530 2540 2550 2560 2570 2580
SLPDGWVYCH ADGSQFDSSL TPLLLNAVLD VRSFFMEDWW VGREMLENLY AEIVYTPILA
2590 2600 2610 2620 2630 2640
PDGTIFKKFR GNNSGQPSTV VDNTLMVVIA MYYSCCKQGW SEEDIQERLV FFANGDDIIL
2650 2660 2670 2680 2690 2700
AVSDKDTWLY DTLSTSFAEL GLNYNFEERT KKREELWFMS HKAVLVDGIY IPKLEPERIV
2710 2720 2730 2740 2750 2760
SILEWDRSKE LMHRTEAICA SMIEAWGYTE LLQEIRKFYL WLLNKDEFKE LASSGKAPYI
2770 2780 2790 2800 2810 2820
AETALRKLYT DVNAQTSELQ RYLEVLDFNH ADDCCESVSL QSGKEKEGDM DADKDPKKST
2830 2840 2850 2860 2870 2880
SSSKGAGTSS KDVNVGSKGK VVPRLQKITR KMNLPMVEGK IILSLDHLLE YKPNQVDLFN
2890 2900 2910 2920 2930 2940
TRATRTQFEA WYNAVKDEYE LDDEQMGVVM NGFMVWCIDN GTSPDANGVW VMMDGEEQIE
2950 2960 2970 2980 2990 3000
YPLKPIVENA KPTLRQIMHH FSDAAEAYIE MRNSESPYMP RYGLLRNLRD RELARYAFDF
3010 3020 3030 3040 3050 3060
YEVTSKTPNR AREAIAQMKA AALSGVNNKL FGLDGNISTN SENTERHTAR DVNQNMHTLL
GMGPPQ
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P21231 in FASTA format |
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