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UniProtKB/Swiss-Prot entry P20966


[Entry info] [Name and origin] [References] [Comments] [Cross-references] [Keywords] [Features] [Sequence] [Tools]

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Entry information
Entry name PTFBC_ECOLI
Primary accession number P20966
Secondary accession numbers None
Integrated into Swiss-Prot on February 1, 1991
Sequence was last modified on February 1, 1991 (Sequence version 1)
Annotations were last modified on    November 25, 2008 (Entry version 82)
Name and origin of the protein
Protein name PTS system fructose-specific EIIBC component
Synonym EIIBC-Fru
Includes Fructose-specific phosphotransferase enzyme IIB component
     (EC 2.7.1.69)
     (PTS system fructose-specific EIIB component)
     (EIII-Fru)
Fructose permease IIC component
     (PTS system fructose-specific EIIC component)
Gene name
Name: fruA
Synonyms: ptsF
OrderedLocusNames: b2167, JW2154
From
Escherichia coli (strain K12) [TaxID: 83333] [HAMAP proteome]
Taxonomy Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacteriales; Enterobacteriaceae; Escherichia.
Protein existence 4: Predicted;
References
[1]
NUCLEOTIDE SEQUENCE [GENOMIC DNA].
STRAIN=K12;
PubMed=3076173 [NCBI, ExPASy, EBI, Israel, Japan]
Prior T.I., Kornberg H.L.;
"Nucleotide sequence of fruA, the gene specifying enzyme IIfru of the phosphoenolpyruvate-dependent sugar phosphotransferase system in Escherichia coli K12.";
J. Gen. Microbiol. 134:2757-2768(1988).
[2]
NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
STRAIN=K12 / BHB2600;
Richterich P., Lakey N., Gryan G., Jaehn L., Mintz L., Robison K., Church G.M.;
"Automated multiplex sequencing of the E.coli genome.";
Submitted (OCT-1993) to the EMBL/GenBank/DDBJ databases.
[3]
NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
STRAIN=K12 / MG1655 / ATCC 47076;
DOI=10.1126/science.277.5331.1453; PubMed=9278503 [NCBI, ExPASy, EBI, Israel, Japan]
Blattner F.R., Plunkett G. III, Bloch C.A., Perna N.T., Burland V., Riley M., Collado-Vides J., Glasner J.D., Rode C.K., Mayhew G.F., Gregor J., Davis N.W., Kirkpatrick H.A., Goeden M.A., Rose D.J., Mau B., Shao Y.;
"The complete genome sequence of Escherichia coli K-12.";
Science 277:1453-1474(1997).
[4]
NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
STRAIN=K12 / W3110 / ATCC 27325 / DSM 5911;
DOI=10.1038/msb4100049; PubMed=16738553 [NCBI, ExPASy, EBI, Israel, Japan]
Hayashi K., Morooka N., Yamamoto Y., Fujita K., Isono K., Choi S., Ohtsubo E., Baba T., Wanner B.L., Mori H., Horiuchi T.;
"Highly accurate genome sequences of Escherichia coli K-12 strains MG1655 and W3110.";
Mol. Syst. Biol. 2:E1-E5(2006).
[5]
NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA] OF 1-365.
STRAIN=K12 / W3110 / ATCC 27325 / DSM 5911;
DOI=10.1093/dnares/3.6.379; PubMed=9097040 [NCBI, ExPASy, EBI, Israel, Japan]
Itoh T., Aiba H., Baba T., Fujita K., Hayashi K., Inada T., Isono K., Kasai H., Kimura S., Kitakawa M., Kitagawa M., Makino K., Miki T., Mizobuchi K., Mori H., Mori T., Motomura K., Nakade S., Nakamura Y., Nashimoto H., Nishio Y., Oshima T., Saito N., Sampei G., Seki Y., Sivasundaram S., Tagami H., Takeda J., Takemoto K., Wada C., Yamamoto Y., Horiuchi T.;
"A 460-kb DNA sequence of the Escherichia coli K-12 genome corresponding to the 40.1-50.0 min region on the linkage map.";
DNA Res. 3:379-392(1996).
[6]
NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 1-74.
STRAIN=K12;
PubMed=1981619 [NCBI, ExPASy, EBI, Israel, Japan]
Orchard L.M.D., Kornberg H.L.;
"Sequence similarities between the gene specifying 1-phosphofructokinase (fruK), genes specifying other kinases in Escherichia coli K12, and lacC of Staphylococcus aureus.";
Proc. R. Soc. Lond., B, Biol. Sci. 242:87-90(1990).
[7]
SUBCELLULAR LOCATION.
STRAIN=K12 / MG1655 / ATCC 47076;
DOI=10.1126/science.1109730; PubMed=15919996 [NCBI, ExPASy, EBI, Israel, Japan]
Daley D.O., Rapp M., Granseth E., Melen K., Drew D., von Heijne G.;
"Global topology analysis of the Escherichia coli inner membrane proteome.";
Science 308:1321-1323(2005).
Comments
  • FUNCTION: The phosphoenolpyruvate-dependent sugar phosphotransferase system (sugar PTS), a major carbohydrate active -transport system, catalyzes the phosphorylation of incoming sugar substrates concomitantly with their translocation across the cell membrane. This system is involved in fructose transport.
  • CATALYTIC ACTIVITY: Protein EIIB N(pi)-phospho-L-histidine/cysteine + sugar = protein EIIB + sugar phosphate.
  • SUBCELLULAR LOCATION: Cell inner membrane; Multi-pass membrane protein.
  • DOMAIN: The EIIB domain is phosphorylated by phospho-EIIA on a cysteinyl or histidyl residue, depending on the transported sugar. Then, it transfers the phosphoryl group to the sugar substrate concomitantly with the sugar uptake processed by the EIIC domain.
  • DOMAIN: The EIIC domain forms the PTS system translocation channel and contains the specific substrate-binding site.
  • SIMILARITY: Contains 1 PTS EIIB type-2 domain.
  • SIMILARITY: Contains 1 PTS EIIC type-2 domain.
Copyright
Copyrighted by the UniProt Consortium, see http://www.uniprot.org/terms. Distributed under the Creative Commons Attribution-NoDerivs License.
Cross-references
Sequence databases
EMBL
M23196; AAA62624.1; -; Genomic_DNA.[EMBL / GenBank / DDBJ] [CoDingSequence]
U00007; AAA60524.1; -; Genomic_DNA.[EMBL / GenBank / DDBJ] [CoDingSequence]
U00096; AAC75228.1; -; Genomic_DNA.[EMBL / GenBank / DDBJ] [CoDingSequence]
AP009048; BAA15976.2; -; Genomic_DNA.[EMBL / GenBank / DDBJ] [CoDingSequence]
X53948; -; NOT_ANNOTATED_CDS; Genomic_DNA.[EMBL / GenBank / DDBJ]
PIR A34962; A34962.
RefSeq AP_002764.1; -.
NP_416672.1; -.
3D structure databases
ModBase P20966.
Enzyme and pathway databases
BioCyc EcoCyc:FRUA-MON; -.
Organism-specific databases
EchoBASE EB0332; -.
EcoGene EG10336; fruA.
Ontologies
GO
GO:0016021; Cellular component: integral to membrane (inferred from electronic annotation from InterPro).
GO:0005886; Cellular component: plasma membrane (inferred from electronic annotation from UniProtKB-KW).
GO:0016301; Molecular function: kinase activity (inferred from electronic annotation from UniProtKB-KW).
GO:0008982; Molecular function: protein-N(PI)-phosphohistidine-sugar phosphotransferase activity (inferred from electronic annotation from InterPro).
GO:0005351; Molecular function: sugar:hydrogen symporter activity (inferred from electronic annotation from InterPro).
GO:0009401; Biological process: phosphoenolpyruvate-dependent sugar phosphotransferase system (inferred from electronic annotation from InterPro).
QuickGo view.
Family and domain databases
InterPro IPR013011; PTS_EIIB_2.
IPR003352; PTS_EIIC.
IPR013014; PTS_EIIC_2.
IPR003353; PTS_IIB_fruc.
IPR006327; PTS_IIC_fruc.
Graphical view of domain structure.
Pfam PF02378; PTS_EIIC; 1.
PF02379; PTS_IIB_fruc; 1.
Pfam graphical view of domain structure.
TIGRFAMs TIGR00829; FRU; 1.
TIGR01427; PTS_IIC_fructo; 1.
PROSITE PS51099; PTS_EIIB_TYPE_2; 1.
PS51104; PTS_EIIC_TYPE_2; 1.
PROSITE graphical view of domain structure (profiles).
ProtoNet P20966.
Genome annotation databases
GeneID 946672; -.
GenomeReviews U00096_GR; b2167.
AP009048_GR; JW2154.
KEGG ecj:JW2154; -.
eco:b2167; -.
Phylogenomic databases
HOGENOM P20966; -.
Genome annotation databases
CMR P20966; b2167.
Other
UniRef View cluster of proteins with at least 50% / 90% / 100% identity.
Keywords
Cell inner membrane; Cell membrane; Complete proteome; Kinase; Membrane; Phosphotransferase system; Repeat; Sugar transport; Transferase; Transmembrane; Transport.
Features
SEVIEWER logo Feature table viewer FT aligner logo Feature aligner
KeyFrom   To Length Description FTId
CHAIN   1   563  563     PTS system fructose-specific EIIBC component. PRO_0000186508
TRANSMEM   236   256  21     Potential. 
TRANSMEM   274   294  21     Potential. 
TRANSMEM   304   324  21     Potential. 
TRANSMEM   349   369  21     Potential. 
TRANSMEM   382   402  21     Potential. 
TRANSMEM   430   450  21     Potential. 
TRANSMEM   463   483  21     Potential. 
TRANSMEM   489   509  21     Potential. 
TRANSMEM   518   538  21     Potential. 
DOMAIN   104   201  98     PTS EIIB type-2. 
DOMAIN   226   561  336     PTS EIIC type-2. 
ACT_SITE   112   112        Phosphocysteine intermediate; for EIIB activity (By similarity). 
Sequence information
Length: 563 AA [This is the length of the unprocessed precursor] Molecular weight: 57519 Da [This is the MW of the unprocessed precursor] CRC64: AC066A96A94DBA05 [This is a checksum on the sequence]
        10         20         30         40         50         60 
MKTLLIIDAN LGQARAYMAK TLLGAAARKA KLEIIDNPND AEMAIVLGDS IPNDSALNGK 

        70         80         90        100        110        120 
NVWLGDISRA VAHPELFLSE AKGHAKPYTA PVAATAPVAA SGPKRVVAVT ACPTGVAHTF 

       130        140        150        160        170        180 
MAAEAIETEA KKRGWWVKVE TRGSVGAGNA ITPEEVAAAD LVIVAADIEV DLAKFAGKPM 

       190        200        210        220        230        240 
YRTSTGLALK KTAQELDKAV AEATPYEPAG KAQTATTESK KESAGAYRHL LTGVSYMLPM 

       250        260        270        280        290        300 
VVAGGLCIAL SFAFGIEAFK EPGTLAAALM QIGGGSAFAL MVPVLAGYIA FSIADRPGLT 

       310        320        330        340        350        360 
PGLIGGMLAV STGSGFIGGI IAGFLAGYIA KLISTQLKLP QSMEALKPIL IIPLISSLVV 

       370        380        390        400        410        420 
GLAMIYLIGK PVAGILEGLT HWLQTMGTAN AVLLGAILGG MMCTDMGGPV NKAAYAFGVG 

       430        440        450        460        470        480 
LLSTQTYGPM AAIMAAGMVP PLAMGLATMV ARRKFDKAQQ EGGKAALVLG LCFISEGAIP 

       490        500        510        520        530        540 
FAARDPMRVL PCCIVGGALT GAISMAIGAK LMAPHGGLFV LLIPGAITPV LGYLVAIIAG 

       550        560 
TLVAGLAYAF LKRPEVDAVA KAA 

P20966 in FASTA format

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View entry in raw text format (no links)
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