NUCLEOTIDE SEQUENCE [GENOMIC DNA].
PubMed=3308458 [NCBI, ExPASy, EBI, Israel, Japan]
Cole S.T.;
"Nucleotide sequence and comparative analysis of the frd operon encoding the fumarate reductase of Proteus vulgaris. Extensive sequence divergence of the membrane anchors and absence of an frd-linked ampC cephalosporinase gene.";
Eur. J. Biochem. 167:481-488(1987).

Comments

FUNCTION: Two distinct, membrane-bound, FAD-containing enzymes are responsible for the catalysis of fumarate and succinate interconversion; the fumarate reductase is used in anaerobic growth, and the succinate dehydrogenase is used in aerobic growth.
CATALYTIC ACTIVITY: Succinate + acceptor = fumarate + reduced acceptor.
COFACTOR: Binds 1 FAD covalently per subunit.
SUBUNIT: Fumarate dehydrogenase forms part of an enzyme complex containing four subunits: a flavoprotein, an iron-sulfur, and two hydrophobic anchor proteins.
SIMILARITY: Belongs to the FAD-dependent oxidoreductase 2 family. FRD/SDH subfamily.

Copyright

Copyrighted by the UniProt Consortium, see http://www.uniprot.org/terms. Distributed under the Creative Commons Attribution-NoDerivs License.

Cross-references

Sequence databases

EMBL

X06144; CAA29501.1; -; Genomic_DNA.

[EMBL / GenBank / DDBJ] [CoDingSequence]

PIR

S00107; RDEBFV.

3D structure databases

HSSP

P00363; 1KF6. [HSSP ENTRY / PDB]

SMR

P20922; 1-577.

ModBase

P20922.

Family and domain databases

InterPro

IPR003953; FAD_bind2_N.
IPR013027; FAD_pyr_nucl-diS_OxRdtase.
IPR003952; FRD_SDH_FAD_BS.
IPR004112; Fum_Rdtase/Succ_DHase_flav_C.
IPR005884; Fum_red_fp.
IPR001100; Pyr_nuc-diS_OxRdtase.
IPR014006; Succ_Dhase_frdA_Gneg.
Graphical view of domain structure.

Pfam

PF00890; FAD_binding_2; 1.
PF02910; Succ_DH_flav_C; 1.
Pfam graphical view of domain structure.

PRINTS

PR00368; FADPNR.
PR00411; PNDRDTASEI.

TIGRFAMs

TIGR01176; fum_red_Fp; 1.
TIGR01812; sdhA_frdA_Gneg; 1.

PROSITE

PS00504; FRD_SDH_FAD_BINDING; 1.

Other

View cluster of proteins with at least 50% / 90% / 100% identity.

Keywords

Electron transport; FAD; Flavoprotein; Oxidoreductase; Transport.

Features

Feature table viewer

`Key`	`From To`	`Length`	`Description`	`FTId`
`CHAIN`	`1 598`	`598`	`Fumarate reductase flavoprotein subunit.`	`PRO_0000158666`
`NP_BIND`	`9 23`	`15`	`FAD (Potential).`
`ACT_SITE`	`233 233`		`By similarity.`
`ACT_SITE`	`249 249`		`By similarity.`
`MOD_RES`	`45 45`		`Tele-8alpha-FAD histidine (By similarity).`

Sequence information

Length: 598 AA [This is the length of the unprocessed precursor]

Molecular weight: 66142 Da [This is the MW of the unprocessed precursor]

CRC64: AB205F8CEAA186AD [This is a checksum on the sequence]

        10         20         30         40         50         60 
MQTFNADIAI IGAGGAGLRA AIAAAEANPQ LKIALISKVY PMRSHTVAAE GGSAAVTQAH 

        70         80         90        100        110        120 
DSYDFHFNDT VSGGDWLCEQ DVVDYFVEHC PTEMTQLELW GCPWSRKEDG SVNVRRFGGM 

       130        140        150        160        170        180 
KIERTWFAAD KTGFHMLHTL FQTSLKYPQI QRFDEHFVLD ILVDEGHARG VVAINMMEGT 

       190        200        210        220        230        240 
KVQIRANAVI MATGGAGRVY RFNTNGGIVT GDGMGIALRH GVPLRDMEFV QYHPTGLPGS 

       250        260        270        280        290        300 
GILMTEGCRG EGGILVNKDG YRYLQDYGLG PETPLGKPEN KYMELGPRDK VSQAFWHEWR 

       310        320        330        340        350        360 
AGRTIKTHRG DVVHLDLRHL GAKKLHERLP FICELAKAYV GVDPVNEPIP VRPTAHYTMG 

       370        380        390        400        410        420 
GIETNQRTET RIKGLFAVGE CSSVGLHGAN RLGSNSLAEL VVFGRLAGEE AVRRAQEATP 

       430        440        450        460        470        480 
ANASALDAQT RDIEDNLKKL MNQKGSENWA QIRDEMGEAM EEGCGIYRTP ELMQKTIDKL 

       490        500        510        520        530        540 
TELKERFKHV EIKDTSSVFN TDLLYKIELG FGLDVAECMA HSAFNRKESR GAHQRLDEGC 

       550        560        570        580        590 
TERDDVNFLK HTLAFYNPEG APRLEYSDVK ITKSAPAKRV YGGEATAQDK QNKEKANG

P20922 in FASTA format

View entry in original UniProtKB/Swiss-Prot format
View entry in raw text format (no links)
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	BLAST submission on ExPASy/SIB or at NCBI (USA)		Sequence analysis tools: ProtParam, ProtScale, Compute pI/Mw, PeptideMass, PeptideCutter, Dotlet (Java)
	ScanProsite, MotifScan		Submit a homology modeling request to SWISS-MODEL
	NPSA Sequence analysis tools