ExPASy logo ExPASy Home page Site Map Search ExPASy Contact us Swiss-Prot
Notice: This page will be replaced with www.uniprot.org. Please send us your feedback!
Search for

UniProtKB/Swiss-Prot entry P20366


[Entry info] [Name and origin] [References] [Comments] [Cross-references] [Keywords] [Features] [Sequence] [Tools]

Note: most headings are clickable, even if they don't appear as links. They link to the user manual or other documents.
Entry information
Entry name TKN1_HUMAN
Primary accession number P20366
Secondary accession numbers O60600 O60601 Q00072 Q53GH4
Integrated into Swiss-Prot on February 1, 1991
Sequence was last modified on February 1, 1991 (Sequence version 1)
Annotations were last modified on    July 1, 2008 (Entry version 92)
Name and origin of the protein
Protein name Protachykinin-1 [Precursor]
Synonym PPT
Contains Substance P
Neurokinin A
     (NKA)
     (Substance K)
     (Neuromedin L)
Neuropeptide K
     (NPK)
Neuropeptide gamma
C-terminal-flanking peptide
Gene name
Name: TAC1
Synonyms: NKA, NKNA, TAC2
From
Homo sapiens (Human) [TaxID: 9606] 
Taxonomy Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini; Catarrhini; Hominidae; Homo.
Protein existence 1: Evidence at protein level;
References
[1]
NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM BETA).
DOI=10.1016/0014-5793(86)81534-4; PubMed=3770210 [NCBI, ExPASy, EBI, Israel, Japan]
Harmar A.J., Armstrong A., Pascall J.C., Chapman K., Rosie R., Curtis A., Going J., Edwards C.R.W., Fink G.;
"cDNA sequence of human beta-preprotachykinin, the common precursor to substance P and neurokinin A.";
FEBS Lett. 208:67-72(1986).
[2]
NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM BETA).
TISSUE=Brain;
Tan A., Too H.P.;
Submitted (OCT-1995) to the EMBL/GenBank/DDBJ databases.
[3]
NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM BETA).
TISSUE=Small intestine;
Suzuki Y., Sugano S., Totoki Y., Toyoda A., Takeda T., Sakaki Y., Tanaka A., Yokoyama S.;
Submitted (APR-2005) to the EMBL/GenBank/DDBJ databases.
[4]
NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM BETA).
TISSUE=Brain;
DOI=10.1101/gr.2596504; PubMed=15489334 [NCBI, ExPASy, EBI, Israel, Japan]
The MGC Project Team;
"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC).";
Genome Res. 14:2121-2127(2004).
[5]
NUCLEOTIDE SEQUENCE [MRNA] OF 36-122 (ISOFORMS BETA AND GAMMA).
TISSUE=Testis;
PubMed=1708336 [NCBI, ExPASy, EBI, Israel, Japan]
Chiwakata C., Brackmann B., Hunt N., Davidoff M., Schulze W., Ivell R.;
"Tachykinin (substance-P) gene expression in Leydig cells of the human and mouse testis.";
Endocrinology 128:2441-2448(1991).
[6]
PROTEIN SEQUENCE OF 98-107.
PubMed=3038549 [NCBI, ExPASy, EBI, Israel, Japan]
Theodorsson-Norheim E., Joernvall H., Andersson M., Norheim I., Oeberg K., Jacobsson G.;
"Isolation and characterization of neurokinin A, neurokinin A(3-10) and neurokinin A(4-10) from a neutral water extract of a metastatic ileal carcinoid tumour.";
Eur. J. Biochem. 166:693-697(1987).
[7]
NUCLEOTIDE SEQUENCE [MRNA] OF 36-122 (ISOFORMS ALPHA; BETA AND DELTA).
TISSUE=Blood, and Brain;
PubMed=9846828 [NCBI, ExPASy, EBI, Israel, Japan]
Lai J.P., Douglas S.D., Rappaport E., Wu J.M., Ho W.Z.;
"Identification of a delta isoform of preprotachykinin mRNA in human mononuclear phagocytes and lymphocytes.";
J. Neuroimmunol. 91:121-128(1998).
[8]
PROTEIN SEQUENCE OF 111-126.
TISSUE=Adrenal medulla;
DOI=10.1016/0196-9781(90)90007-R; PubMed=2284201 [NCBI, ExPASy, EBI, Israel, Japan]
McGregor G.P., Conlon J.M.;
"Characterization of the C-terminal flanking peptide of human beta-preprotachykinin.";
Peptides 11:907-910(1990).
[9]
STRUCTURE BY NMR OF 72-107.
DOI=10.1021/bi052287o; PubMed=16503654 [NCBI, ExPASy, EBI, Israel, Japan]
Dike A., Cowsik S.M.;
"Three-dimensional structure of neuropeptide K bound to dodecylphosphocholine micelles.";
Biochemistry 45:2994-3004(2006).
Comments
Copyright
Copyrighted by the UniProt Consortium, see http://www.uniprot.org/terms. Distributed under the Creative Commons Attribution-NoDerivs License.
Cross-references
Sequence databases
EMBL
X54469; CAA38351.1; -; mRNA.[EMBL / GenBank / DDBJ] [CoDingSequence]
U37529; AAA79195.1; -; mRNA.[EMBL / GenBank / DDBJ] [CoDingSequence]
AK222957; BAD96677.1; -; mRNA.[EMBL / GenBank / DDBJ] [CoDingSequence]
BC018047; AAH18047.1; -; mRNA.[EMBL / GenBank / DDBJ] [CoDingSequence]
M68906; AAA60159.1; -; mRNA.[EMBL / GenBank / DDBJ] [CoDingSequence]
M68907; AAA60160.1; -; mRNA.[EMBL / GenBank / DDBJ] [CoDingSequence]
AF050655; AAC15701.1; -; mRNA.[EMBL / GenBank / DDBJ] [CoDingSequence]
AF050656; AAC15702.1; -; mRNA.[EMBL / GenBank / DDBJ] [CoDingSequence]
AF050658; AAC15704.1; -; mRNA.[EMBL / GenBank / DDBJ] [CoDingSequence]
PIR A24805; SPHUB.
RefSeq NP_003173.1; -.
NP_054702.1; -.
NP_054704.1; -.
UniGene Hs.2563
3D structure databases
PDB
2B19; NMR; -; A=72-107.[ExPASy / RCSB / EBI]
PDBsum 2B19; -.
ModBase P20366.
Organism-specific databases
H-InvDB HIX0006874; -.
HGNC HGNC:11517; TAC1.
GeneLynx TAC1; Homo sapiens.
GenAtlas TAC1.
HPA CAB004588; -.
MIM 162320; gene. [NCBI / EBI]
PharmGKB PA26899; -.
GeneCards P20366.
Gene expression databases
ArrayExpress P20366; -.
CleanEx HS_TAC1; -.
GermOnline ENSG00000006128; Homo sapiens.
Ontologies
GO
GO:0005615; Cellular component: extracellular space (traceable author statement from ProtInc).
GO:0007267; Biological process: cell-cell signaling (traceable author statement from ProtInc).
GO:0009582; Biological process: detection of abiotic stimulus (traceable author statement from ProtInc).
GO:0007204; Biological process: elevation of cytosolic calcium ion concentration (inferred from direct assay from UniProtKB).
GO:0007320; Biological process: insemination (traceable author statement from ProtInc).
QuickGo view.
Family and domain databases
InterPro IPR008216; Protachykinin.
IPR013055; Tachy_Neuro_lke_CS.
IPR002040; Tachy_Neurokinin.
IPR008215; Tachykinin.
Graphical view of domain structure.
PANTHER PTHR11250; Protachykinin; 1.
Pfam PF02202; Tachykinin; 2.
Pfam graphical view of domain structure.
PRINTS PR01829; PROTACHYKNIN.
ProDom PD005598; Protachykinin; 1.
[Domain structure / List of seq. sharing at least 1 domain]
SMART SM00203; TK; 2.
SMART graphical view of domain structure.
PROSITE PS00267; TACHYKININ; 2.
BLOCKS P20366.
Genome annotation databases
Ensembl ENSG00000006128; Homo sapiens. [Contig view]
GeneID 6863; -.
KEGG hsa:6863; -.
Phylogenomic databases
HOGENOM P20366; -.
HOVERGEN P20366; -.
Other
DrugBank DB00626; Bacitracin.
LinkHub P20366; -.
SOURCE TAC1; Homo sapiens.
ProtoNet P20366.
UniRef View cluster of proteins with at least 50% / 90% / 100% identity.
Keywords
3D-structure; Alternative splicing; Amidation; Cleavage on pair of basic residues; Direct protein sequencing; Neuropeptide; Neurotransmitter; Secreted; Signal.
Features
SEVIEWER logo Feature table viewer FT aligner logo Feature aligner
KeyFrom   To Length Description FTId
SIGNAL   1    19  19     Potential. 
PROPEP   20    56  37     Potential. PRO_0000033529
PEPTIDE   58    68  11     Substance P. PRO_0000033530
PEPTIDE   72   107  36     Neuropeptide K. PRO_0000033531
PEPTIDE   72    73  2     Neuropeptide gamma, 1st part. PRO_0000033532
PEPTIDE   89   107  19     Neuropeptide gamma, 2nd part. PRO_0000033533
PEPTIDE   98   107  10     Neurokinin A. PRO_0000033534
PEPTIDE   111   126  16     C-terminal-flanking peptide. PRO_0000033535
MOD_RES   68    68        Methionine amide. 
MOD_RES   107   107        Methionine amide. 
VAR_SEQ   74    88        Missing (in isoform Gamma and isoform Delta). VSP_006375
VAR_SEQ   97   114        Missing (in isoform Alpha and isoform Delta). VSP_006376
VAR_SEQ   115   115        V -> M (in isoform Alpha and isoform Delta). VSP_006377
CONFLICT   32    32        S -> P (in Ref. 3; BAD96677). 
Sequence information
Length: 129 AA [This is the length of the unprocessed precursor] Molecular weight: 15003 Da [This is the MW of the unprocessed precursor] CRC64: 51412C1692368DE4 [This is a checksum on the sequence]
        10         20         30         40         50         60 
MKILVALAVF FLVSTQLFAE EIGANDDLNY WSDWYDSDQI KEELPEPFEH LLQRIARRPK 

        70         80         90        100        110        120 
PQQFFGLMGK RDADSSIEKQ VALLKALYGH GQISHKRHKT DSFVGLMGKR ALNSVAYERS 


AMQNYERRR 

P20366 in FASTA format

View entry in original UniProtKB/Swiss-Prot format
View entry in raw text format (no links)
Report form for errors/updates in this UniProtKB/Swiss-Prot entry

BLAST logo BLAST submission on ExPASy/SIB
or at NCBI (USA)
Tools Sequence analysis tools: ProtParam, ProtScale, Compute pI/Mw, PeptideMass, PeptideCutter, Dotlet (Java)
PROSITE logo ScanProsite, MotifScan SWISS-MODEL Submit a homology modeling request to SWISS-MODEL
NPSA logo NPSA Sequence analysis tools

ExPASy logo ExPASy Home page Site Map Search ExPASy Contact us Swiss-Prot
 Hosted by ch flag SIB Switzerland Mirror sites: Australia  Brazil  Canada  China  Korea
Notice: This page will be replaced with www.uniprot.org. Please send us your feedback!