[1]	NUCLEOTIDE SEQUENCE [MRNA] (ISOFORMS 1 AND 2). PubMed=7515060 [NCBI, ExPASy, EBI, Israel, Japan] Chen B.P.C., Liang G., Whelan J., Hai T.; "ATF3 and ATF3 delta Zip. Transcriptional repression versus activation by alternatively spliced isoforms."; J. Biol. Chem. 269:15819-15826(1994).
[2]	NUCLEOTIDE SEQUENCE [GENOMIC DNA], AND VARIANT MET-38. NIEHS SNPs program; Submitted (OCT-2003) to the EMBL/GenBank/DDBJ databases.
[3]	NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1). TISSUE=Muscle; DOI=10.1101/gr.2596504; PubMed=15489334 [NCBI, ExPASy, EBI, Israel, Japan] The MGC Project Team; "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."; Genome Res. 14:2121-2127(2004).
[4]	NUCLEOTIDE SEQUENCE [MRNA] OF 25-181. PubMed=2516827 [NCBI, ExPASy, EBI, Israel, Japan] Hai T., Liu F., Coukos W.J., Green M.R.; "Transcription factor ATF cDNA clones: an extensive family of leucine zipper proteins able to selectively form DNA-binding heterodimers."; Genes Dev. 3:2083-2090(1989).
[5]	ERRATUM. Hai T., Liu F., Coukos W.J., Green M.R.; Genes Dev. 4:682-682(1990).
[6]	SPLICE ISOFORM(S) THAT ARE POTENTIAL NMD TARGET(S). DOI=10.1186/gb-2004-5-2-r8; PubMed=14759258 [NCBI, ExPASy, EBI, Israel, Japan] Hillman R.T., Green R.E., Brenner S.E.; "An unappreciated role for RNA surveillance."; Genome Biol. 5:RESEARCH008.1-RESEARCH008.16(2004).

Comments

FUNCTION: This protein binds the cAMP response element (CRE) (consensus: 5'-GTGACGT[AC][AG]-3'), a sequence present in many viral and cellular promoters. Represses transcription from promoters with ATF sites. It may repress transcription by stabilizing the binding of inhibitory cofactors at the promoter. Isoform 2 activates transcription presumably by sequestering inhibitory cofactors away from the promoters.
SUBUNIT: Binds DNA as a homodimer or a heterodimer.
INTERACTION:
Self; NbExp=1; IntAct=EBI-712767, EBI-712767;
Q14192:FHL2; NbExp=1; IntAct=EBI-712767, EBI-701903;
Q04206:RELA; NbExp=1; IntAct=EBI-712767, EBI-73886;
SUBCELLULAR LOCATION: Nucleus.

ALTERNATIVE PRODUCTS: 2 named isoforms [FASTA] produced by alternative splicing.

Name	1
Synonyms	ATF3
Isoform ID	P18847-1
This is the isoform sequence displayed in this entry.

Name	2
Synonyms	ATF3-delta-Zip
Isoform ID	P18847-2
Note: May be produced at very low levels due to a premature stop codon in the mRNA, leading to nonsense-mediated mRNA decay.
Features which should be applied to build the isoform sequence: VSP_000592.

SIMILARITY: Belongs to the bZIP family. ATF subfamily.
SIMILARITY: Contains 1 bZIP domain.
WEB RESOURCE: Name=NIEHS-SNPs; URL="http://egp.gs.washington.edu/data/atf3/";.

Copyright

Copyrighted by the UniProt Consortium, see http://www.uniprot.org/terms. Distributed under the Creative Commons Attribution-NoDerivs License.

Cross-references

Sequence databases

EMBL

L19871; AAA20506.1; -; mRNA.	[EMBL / GenBank / DDBJ] [CoDingSequence]
AY426987; AAQ93358.1; -; Genomic_DNA.	[EMBL / GenBank / DDBJ] [CoDingSequence]
BC006322; AAH06322.1; -; mRNA.	[EMBL / GenBank / DDBJ] [CoDingSequence]

IPI

IPI00002502; -.
IPI00413177; -.

PIR

A54025; A54025.
B54025; B54025.
C34223; C34223.

RefSeq

NP_001025458.1; -.
NP_001665.1; -.
NP_004015.4; -.

UniGene

Hs.460

3D structure databases

HSSP

P01100; 1FOS. [HSSP ENTRY / PDB]

ModBase

P18847.

Protein-protein interaction databases

DIP

DIP:254N; -.
DIP:344N; -.

IntAct

P18847; 10.

Enzyme and pathway databases

Pathway_Interaction_DB

smad2_3nuclearpathway; Regulation of nuclear SMAD2/3 signaling.

Organism-specific databases

GC01P210805; -.

HIX0001571; -.

HGNC:785; ATF3.

HPA001562; -.

603148; gene. [NCBI / EBI]

PharmGKB

PA25085; -.

Gene expression databases

P18847; -.

P18847; -.

HS_ATF3; -.

ENSG00000162772; Homo sapiens.

Ontologies

GO:0005730;	Cellular component: nucleolus (inferred from direct assay from HPA).
GO:0042802;	Molecular function: identical protein binding (inferred from physical interaction from IntAct).
GO:0046983;	Molecular function: protein dimerization activity (inferred from electronic annotation from InterPro).
GO:0043565;	Molecular function: sequence-specific DNA binding (inferred from electronic annotation from InterPro).
GO:0003714;	Molecular function: transcription corepressor activity (traceable author statement from ProtInc).
GO:0003700;	Molecular function: transcription factor activity (traceable author statement from ProtInc).
GO:0006355;	Biological process: regulation of transcription, DNA-dependent (inferred from electronic annotation from UniProtKB-KW).
GO:0006350;	Biological process: transcription (inferred from electronic annotation from UniProtKB-KW).
QuickGo view.

Family and domain databases

InterPro

IPR011700; bZIP_2.
IPR000837; Leuzip_Fos.
IPR004827; TF_bZIP.
Graphical view of domain structure.

Pfam

PF07716; bZIP_2; 1.
Pfam graphical view of domain structure.

PRINTS

PR00042; LEUZIPPRFOS.

SMART

SM00338; BRLZ; 1.
SMART graphical view of domain structure.

PROSITE

PS50217; BZIP; 1.
PS00036; BZIP_BASIC; 1.
PROSITE graphical view of domain structure (profiles).

Proteomic databases

PRIDE

P18847; -.

Genome annotation databases

Ensembl

ENSG00000162772; Homo sapiens. [Contig view]

GeneID

467; -.

KEGG

hsa:467; -.

Phylogenomic databases

HOGENOM

P18847; -.

HOVERGEN

P18847; -.

OMA

P18847; ATAIVPC.

Other

1925; -.

ATF3; Homo sapiens.

View cluster of proteins with at least 50% / 90% / 100% identity.

Keywords

Alternative splicing; DNA-binding; Nucleus; Polymorphism; Repressor; Transcription; Transcription regulation.

Features

Feature table viewer

Feature aligner

`Key`	`From To`	`Length`	`Description`	`FTId`
`CHAIN`	`1 181`	`181`	`Cyclic AMP-dependent transcription factor ATF-3.`	`PRO_0000076581`
`DOMAIN`	`114 142`	`29`	`Leucine-zipper.`
`DNA_BIND`	`88 110`	`23`	`Basic motif.`
`VAR_SEQ`	`116 181`		`KESEKLESVNAELKAQIEELKNEKQHLIYMLNLHRPTCIV` `RAQNGRTPEDERNLFIQQIKEGTLQS -> LQY (in isoform 2).`	`VSP_000592`
`VARIANT`	`38 38`	`1`	`T -> M (in dbSNP:rs11571541 [NCBI]).`	`VAR_048442`
`CONFLICT`	`132 132`		`I -> L (in Ref. 4).`

Sequence information

Length: 181 AA [This is the length of the unprocessed precursor]

Molecular weight: 20576 Da [This is the MW of the unprocessed precursor]

CRC64: EC5D8F065EEE2D9C [This is a checksum on the sequence]

        10         20         30         40         50         60 
MMLQHPGQVS ASEVSASAIV PCLSPPGSLV FEDFANLTPF VKEELRFAIQ NKHLCHRMSS 

        70         80         90        100        110        120 
ALESVTVSDR PLGVSITKAE VAPEEDERKK RRRERNKIAA AKCRNKKKEK TECLQKESEK 

       130        140        150        160        170        180 
LESVNAELKA QIEELKNEKQ HLIYMLNLHR PTCIVRAQNG RTPEDERNLF IQQIKEGTLQ 


S

P18847 in FASTA format

View entry in raw text format (no links)
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	BLAST submission on ExPASy/SIB or at NCBI (USA)		Sequence analysis tools: ProtParam, ProtScale, Compute pI/Mw, PeptideMass, PeptideCutter, Dotlet (Java)
	ScanProsite, MotifScan		Submit a homology modeling request to SWISS-MODEL
	NPSA Sequence analysis tools