[1]	NUCLEOTIDE SEQUENCE [MRNA], AND PARTIAL PROTEIN SEQUENCE. TISSUE=Bone marrow; PubMed=1696722 [NCBI, ExPASy, EBI, Israel, Japan] Cherif-Zahar B., Bloy C., le van Kim C., Blanchard D., Bailly P., Hermand P., Salmon C., Cartron J.-P., Colin Y.; "Molecular cloning and protein structure of a human blood group Rh polypeptide."; Proc. Natl. Acad. Sci. U.S.A. 87:6243-6247(1990).
[2]	NUCLEOTIDE SEQUENCE [MRNA]. PubMed=2123099 [NCBI, ExPASy, EBI, Israel, Japan] Avent N.D., Ridgwell K., Tanner M.J.A., Anstee D.J.; "cDNA cloning of a 30 kDa erythrocyte membrane protein associated with Rh (Rhesus)-blood-group-antigen expression."; Biochem. J. 271:821-825(1990).
[3]	NUCLEOTIDE SEQUENCE [MRNA]. DOI=10.1007/BF00222717; PubMed=7916743 [NCBI, ExPASy, EBI, Israel, Japan] Kajii E., Umenishi F., Iwamoto S., Ikemoto S.; "Isolation of a new cDNA clone encoding an Rh polypeptide associated with the Rh blood group system."; Hum. Genet. 91:157-162(1993).
[4]	NUCLEOTIDE SEQUENCE [MRNA] (ISOFORMS RHIV; RHVI AND RHVIII). TISSUE=Bone marrow; PubMed=1379850 [NCBI, ExPASy, EBI, Israel, Japan] le van Kim C., Cherif-Zahar B., Raynal V., Mouro I., Lopez M., Cartron J.-P., Colin Y.; "Multiple Rh messenger RNA isoforms are produced by alternative splicing."; Blood 80:1074-1078(1992).
[5]	NUCLEOTIDE SEQUENCE, AND VARIANTS RHEKH THR-154; RHEFM GLU-233 AND RHEFM VAL-238. Kashiwase K., Ishikawa Y., Uchikawa M.; "The serological profile and molecular basis of the RhE variants (RhEFM and RhEKH) in Japanese."; Submitted (OCT-1998) to the EMBL/GenBank/DDBJ databases.
[6]	NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 1-11. DOI=10.1006/geno.1994.1014; PubMed=8188244 [NCBI, ExPASy, EBI, Israel, Japan] Cherif-Zahar B., le van Kim C., Rouillac C., Raynal V., Cartron J.-P., Colin Y.; "Organization of the gene (RHCE) encoding the human blood group RhCcEe antigens and characterization of the promoter region."; Genomics 19:68-74(1994).
[7]	PROTEIN SEQUENCE OF 2-33. PubMed=3146980 [NCBI, ExPASy, EBI, Israel, Japan] Avent N.D., Ridgwell K., Mawby W.J., Tanner M.J.A., Anstee D.J., Kumpel B.; "Protein-sequence studies on Rh-related polypeptides suggest the presence of at least two groups of proteins which associate in the human red-cell membrane."; Biochem. J. 256:1043-1046(1988).
[8]	PROTEIN SEQUENCE OF 2-17. PubMed=3135863 [NCBI, ExPASy, EBI, Israel, Japan] Bloy C., Blanchard D., Dahr W., Beyreuther K., Salmon C., Cartron J.-P.; "Determination of the N-terminal sequence of human red cell Rh(D) polypeptide and demonstration that the Rh(D), (c), and (E) antigens are carried by distinct polypeptide chains."; Blood 72:661-666(1988).
[9]	PROTEIN SEQUENCE OF 402-409. PubMed=1898705 [NCBI, ExPASy, EBI, Israel, Japan] Suyama K., Goldstein J., Aebersold R., Kent S.; "Regarding the size of Rh proteins."; Blood 77:411-411(1991).
[10]	VARIANTS BLOOD GROUP C AND E. DOI=10.1038/ng0993-62; PubMed=8220426 [NCBI, ExPASy, EBI, Israel, Japan] Mouro I., Colin Y., Cherif-Zahar B., Cartron J.-P., le van Kim C.; "Molecular genetic basis of the human Rhesus blood group system."; Nat. Genet. 5:62-65(1993).

Comments

FUNCTION: May be part of an oligomeric complex which is likely to have a transport or channel function in the erythrocyte membrane.
SUBCELLULAR LOCATION: Membrane; Multi-pass membrane protein.

ALTERNATIVE PRODUCTS: 4 named isoforms [FASTA] produced by alternative splicing.

Name	RHI
Isoform ID	P18577-1
This is the isoform sequence displayed in this entry.

Name	RHIV
Isoform ID	P18577-2
Features which should be applied to build the isoform sequence: VSP_005703, VSP_005704.

Name	RHVI
Isoform ID	P18577-3
Features which should be applied to build the isoform sequence: VSP_005702, VSP_005705.

Name	RHVIII
Isoform ID	P18577-4
Features which should be applied to build the isoform sequence: VSP_005701.

TISSUE SPECIFICITY: Restricted to tissues or cell lines expressing erythroid characters.
POLYMORPHISM: RhCE and RhD are responsible for the RH blood group system. The molecular basis of the E=Rh3/e=Rh5 blood group antigens is a single variation in position 226; Pro-226 corresponds to Rh3 and Ala-226 to Rh5. The molecular basis of the C=Rh2/c=Rh4 blood group antigens is a single variation in position 102; Ser-103 corresponds to Rh2 and Pro-103 to Rh4.
SIMILARITY: Belongs to the ammonium transporter (TC 2.A.49) family. Rh subfamily [view classification].
WEB RESOURCE: Name=BGMUT; Note=Blood group antigen gene mutation database"; URL="http://www.ncbi.nlm.nih.gov/projects/mhc/xslcgi.fcgi?cmd=bgmut/systems_info&system=rh";.
WEB RESOURCE: Name=GeneReviews; URL="http://www.genetests.org/query?gene=RHCE";.

Copyright

Copyrighted by the UniProt Consortium, see http://www.uniprot.org/terms. Distributed under the Creative Commons Attribution-NoDerivs License.

Cross-references

Sequence databases

EMBL

M34015; AAA36567.1; -; mRNA.	[EMBL / GenBank / DDBJ] [CoDingSequence]
X54534; CAA38401.1; -; mRNA.	[EMBL / GenBank / DDBJ] [CoDingSequence]
S57967; AAB26080.1; -; mRNA.	[EMBL / GenBank / DDBJ] [CoDingSequence]
X63095; CAA44809.1; -; mRNA.	[EMBL / GenBank / DDBJ] [CoDingSequence]
X63096; CAA44810.1; -; mRNA.	[EMBL / GenBank / DDBJ] [CoDingSequence]
X63098; CAA44812.1; -; mRNA.	[EMBL / GenBank / DDBJ] [CoDingSequence]
AB018644; BAA33927.1; -; mRNA.	[EMBL / GenBank / DDBJ] [CoDingSequence]
AB018645; BAA33928.1; -; mRNA.	[EMBL / GenBank / DDBJ] [CoDingSequence]
S70456; AAD14061.1; -; Genomic_DNA.	[EMBL / GenBank / DDBJ] [CoDingSequence]

PIR

A30405; A30405.
S78478; S78478.
S78479; S78479.
S78480; S78480.

RefSeq

NP_065231.2; -.
NP_619522.2; -.
NP_619523.2; -.
NP_619524.2; -.

UniGene

Hs.449968

3D structure databases

ModBase

P18577.

Organism-specific databases

HIX0023511; -.

HGNC:10008; RHCE.

RHCE; Homo sapiens.

111690; phenotype. [NCBI / EBI]
111700; gene+phenotype. [NCBI / EBI]

Orphanet

71275; Rh deficiency syndrome.

PharmGKB

PA34386; -.

GeneCards

P18577.

Gene expression databases

ArrayExpress

P18577; -.

CleanEx

HS_RHCE; -.

GermOnline

ENSG00000188672; Homo sapiens.

Ontologies

GO:0005887;	Cellular component: integral to plasma membrane (traceable author statement from ProtInc).
QuickGo view.

Family and domain databases

InterPro

IPR010256; RH_like_transpt.
Graphical view of domain structure.

Pfam

PF00909; Ammonium_transp; 1.
Pfam graphical view of domain structure.

BLOCKS

P18577.

Genome annotation databases

Ensembl

ENSG00000188672; Homo sapiens. [Contig view]

GeneID

6006; -.

KEGG

hsa:6006; -.

Phylogenomic databases

HOVERGEN

P18577; -.

Other

RHCE; Homo sapiens.

View cluster of proteins with at least 50% / 90% / 100% identity.

Keywords

Alternative splicing; Blood group antigen; Direct protein sequencing; Membrane; Polymorphism; Transmembrane.

Features

Feature table viewer

Feature aligner

`Key`	`From To`	`Length`	`Description`	`FTId`
`INIT_MET`	`1 1`		`Removed.`
`CHAIN`	`2 417`	`416`	`Blood group Rh(CE) polypeptide.`	`PRO_0000168189`
`TRANSMEM`	`12 32`	`21`	`Potential.`
`TRANSMEM`	`44 64`	`21`	`Potential.`
`TRANSMEM`	`77 97`	`21`	`Potential.`
`TRANSMEM`	`125 145`	`21`	`Potential.`
`TRANSMEM`	`172 192`	`21`	`Potential.`
`TRANSMEM`	`203 223`	`21`	`Potential.`
`TRANSMEM`	`238 258`	`21`	`Potential.`
`TRANSMEM`	`265 285`	`21`	`Potential.`
`TRANSMEM`	`287 307`	`21`	`Potential.`
`TRANSMEM`	`331 351`	`21`	`Potential.`
`TRANSMEM`	`358 378`	`21`	`Potential.`
`VAR_SEQ`	`163 313`		`Missing (in isoform RHVIII).`	`VSP_005701`
`VAR_SEQ`	`164 268`		`Missing (in isoform RHVI).`	`VSP_005702`
`VAR_SEQ`	`314 354`		`VCCNRVLGIHHISVMHSIFSLLGLLGEITYIVLLVL` `HTVWN -> DWLPGPPQHWGTQLGHRDSSHVWSPDRFAPKSQNME` `STSCG (in isoform RHIV).`	`VSP_005703`
`VAR_SEQ`	`355 417`		`Missing (in isoform RHIV).`	`VSP_005704`
`VAR_SEQ`	`359 417`		`IGFQVLLSIGELSLAIVIALTSGLLTGLLLNLKIWKAPHV` `AKYFDDQVFWKFPHLAVGF -> FAPKSQNMESTSC (in isoform RHVI).`	`VSP_005705`
`VARIANT`	`16 16`	`1`	`W -> C.`	`VAR_006911`
`VARIANT`	`36 36`	`1`	`A -> T (in C(X)/Rh9 antigen).`	`VAR_006912`
`VARIANT`	`41 41`	`1`	`Q -> R (in C(W)/Rh8 antigen).`	`VAR_006913`
`VARIANT`	`60 60`	`1`	`L -> I.`	`VAR_006914`
`VARIANT`	`68 68`	`1`	`N -> S.`	`VAR_006915`
`VARIANT`	`103 103`	`1`	`P -> S (in C/Rh2 antigen).`	`VAR_006916`
`VARIANT`	`154 154`	`1`	`R -> T (in RhEKH).`	`VAR_013301`
`VARIANT`	`226 226`	`1`	`P -> A (in E/Rh5 antigen).`	`VAR_006917`
`VARIANT`	`233 233`	`1`	`Q -> E (in RhEFM).`	`VAR_013302`
`VARIANT`	`238 238`	`1`	`M -> V (in RhEFM).`	`VAR_013303`
`VARIANT`	`245 245`	`1`	`L -> V (in VS antigen).`	`VAR_006918`
`CONFLICT`	`12 12`		`C -> L (in Ref. 7; AA sequence).`
`CONFLICT`	`53 53`		`D -> G (in Ref. 4; CAA44812).`
`CONFLICT`	`61 61`		`G -> C (in Ref. 4; CAA44812).`
`CONFLICT`	`408 409`		`WK -> DI (in Ref. 9; AA sequence).`

Sequence information

Length: 417 AA [This is the length of the unprocessed precursor]

Molecular weight: 45560 Da [This is the MW of the unprocessed precursor]

CRC64: 29D33E778D9053DF [This is a checksum on the sequence]

        10         20         30         40         50         60 
MSSKYPRSVR RCLPLWALTL EAALILLFYF FTHYDASLED QKGLVASYQV GQDLTVMAAL 

        70         80         90        100        110        120 
GLGFLTSNFR RHSWSSVAFN LFMLALGVQW AILLDGFLSQ FPPGKVVITL FSIRLATMSA 

       130        140        150        160        170        180 
MSVLISAGAV LGKVNLAQLV VMVLVEVTAL GTLRMVISNI FNTDYHMNLR HFYVFAAYFG 

       190        200        210        220        230        240 
LTVAWCLPKP LPKGTEDNDQ RATIPSLSAM LGALFLWMFW PSVNSPLLRS PIQRKNAMFN 

       250        260        270        280        290        300 
TYYALAVSVV TAISGSSLAH PQRKISMTYV HSAVLAGGVA VGTSCHLIPS PWLAMVLGLV 

       310        320        330        340        350        360 
AGLISIGGAK CLPVCCNRVL GIHHISVMHS IFSLLGLLGE ITYIVLLVLH TVWNGNGMIG 

       370        380        390        400        410 
FQVLLSIGEL SLAIVIALTS GLLTGLLLNL KIWKAPHVAK YFDDQVFWKF PHLAVGF

P18577 in FASTA format

View entry in original UniProtKB/Swiss-Prot format
View entry in raw text format (no links)
Report form for errors/updates in this UniProtKB/Swiss-Prot entry

	BLAST submission on ExPASy/SIB or at NCBI (USA)		Sequence analysis tools: ProtParam, ProtScale, Compute pI/Mw, PeptideMass, PeptideCutter, Dotlet (Java)
	ScanProsite, MotifScan		Submit a homology modeling request to SWISS-MODEL
	NPSA Sequence analysis tools