ID   POL1_BRSV               Reviewed;        2264 AA.
AC   P18522;
DT   01-NOV-1990, integrated into UniProtKB/Swiss-Prot.
DT   01-NOV-1990, sequence version 1.
DT   08-NOV-2023, entry version 120.
DE   RecName: Full=RNA1 polyprotein;
DE   AltName: Full=P1;
DE   Contains:
DE     RecName: Full=P1A protein;
DE              Short=1A;
DE     AltName: Full=Protease cofactor;
DE   Contains:
DE     RecName: Full=Putative ATP-dependent helicase;
DE              EC=3.6.4.-;
DE     AltName: Full=1B;
DE     AltName: Full=Membrane-binding protein;
DE     AltName: Full=NTP-binding protein;
DE              Short=NTB;
DE   Contains:
DE     RecName: Full=Viral genome-linked protein;
DE     AltName: Full=1C-VPg;
DE   Contains:
DE     RecName: Full=Picornain 3C-like protease;
DE              Short=3C-like protease;
DE              EC=3.4.22.-;
DE     AltName: Full=1D-PRO;
DE   Contains:
DE     RecName: Full=RNA-directed RNA polymerase;
DE              EC=2.7.7.48;
DE     AltName: Full=1E-POL;
OS   Beet ringspot virus (BRSV) (Tomato black ring virus (strain S)).
OC   Viruses; Riboviria; Orthornavirae; Pisuviricota; Pisoniviricetes;
OC   Picornavirales; Secoviridae; Comovirinae; Nepovirus; Nepovirus betae.
OX   NCBI_TaxID=191547;
OH   NCBI_TaxID=4681; Allium porrum (Leek) (Allium ampeloprasum var. porrum).
OH   NCBI_TaxID=4045; Apium graveolens (Celery).
OH   NCBI_TaxID=161934; Beta vulgaris (Sugar beet).
OH   NCBI_TaxID=38871; Fraxinus (ash trees).
OH   NCBI_TaxID=4236; Lactuca sativa (Garden lettuce).
OH   NCBI_TaxID=39639; Narcissus pseudonarcissus (Daffodil).
OH   NCBI_TaxID=3885; Phaseolus vulgaris (Kidney bean) (French bean).
OH   NCBI_TaxID=35938; Robinia pseudoacacia (Black locust).
OH   NCBI_TaxID=23216; Rubus (bramble).
OH   NCBI_TaxID=4081; Solanum lycopersicum (Tomato) (Lycopersicon esculentum).
OH   NCBI_TaxID=4113; Solanum tuberosum (Potato).
OH   NCBI_TaxID=13305; Tulipa.
OH   NCBI_TaxID=3603; Vitis.
RN   [1]
RP   NUCLEOTIDE SEQUENCE [GENOMIC RNA].
RA   Greif C., Hemmer O., Fritsch C.;
RT   "Nucleotide sequence of tomato black ring virus RNA-1.";
RL   J. Gen. Virol. 69:1517-1529(1988).
CC   -!- FUNCTION: Picornain 3C-like protease is a thiol protease that cleaves
CC       the P1 and P2 polyproteins. {ECO:0000250}.
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=a ribonucleoside 5'-triphosphate + RNA(n) = diphosphate +
CC         RNA(n+1); Xref=Rhea:RHEA:21248, Rhea:RHEA-COMP:14527, Rhea:RHEA-
CC         COMP:17342, ChEBI:CHEBI:33019, ChEBI:CHEBI:61557, ChEBI:CHEBI:140395;
CC         EC=2.7.7.48; Evidence={ECO:0000255|PROSITE-ProRule:PRU00539};
CC   -!- SUBCELLULAR LOCATION: [Viral genome-linked protein]: Host endoplasmic
CC       reticulum lumen {ECO:0000250}.
CC   -!- SUBCELLULAR LOCATION: [Putative ATP-dependent helicase]: Host
CC       endoplasmic reticulum membrane {ECO:0000250}; Single-pass membrane
CC       protein {ECO:0000250}.
CC   -!- PTM: Specific enzymatic cleavages by picornain 3C-like protease in vivo
CC       yield mature proteins. Picornain 3C-like protease is autocatalytically
CC       processed (By similarity). {ECO:0000250}.
CC   -!- PTM: VPg is uridylylated by the polymerase and is covalently linked to
CC       the 5'-end of genomic RNA. This uridylylated form acts as a nucleotide-
CC       peptide primer for the polymerase (By similarity). {ECO:0000250}.
CC   -!- SIMILARITY: Belongs to the nepoviruses RNA1 polyprotein family.
CC       {ECO:0000305}.
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DR   EMBL; D00322; BAA00234.1; -; Genomic_RNA.
DR   PIR; JQ0009; GNVVTB.
DR   RefSeq; NP_620112.1; NC_003693.1.
DR   SMR; P18522; -.
DR   MEROPS; C03.025; -.
DR   GeneID; 988049; -.
DR   KEGG; vg:988049; -.
DR   Proteomes; UP000007615; Genome.
DR   GO; GO:0044166; C:host cell endoplasmic reticulum lumen; IEA:UniProtKB-SubCell.
DR   GO; GO:0044167; C:host cell endoplasmic reticulum membrane; IEA:UniProtKB-SubCell.
DR   GO; GO:0016020; C:membrane; IEA:UniProtKB-KW.
DR   GO; GO:0005524; F:ATP binding; IEA:UniProtKB-KW.
DR   GO; GO:0004197; F:cysteine-type endopeptidase activity; IEA:InterPro.
DR   GO; GO:0003723; F:RNA binding; IEA:UniProtKB-KW.
DR   GO; GO:0003724; F:RNA helicase activity; IEA:InterPro.
DR   GO; GO:0003968; F:RNA-dependent RNA polymerase activity; IEA:UniProtKB-KW.
DR   GO; GO:0006351; P:DNA-templated transcription; IEA:InterPro.
DR   GO; GO:0006508; P:proteolysis; IEA:UniProtKB-KW.
DR   GO; GO:0039694; P:viral RNA genome replication; IEA:InterPro.
DR   Gene3D; 1.20.960.20; -; 1.
DR   Gene3D; 3.30.70.270; -; 1.
DR   InterPro; IPR043502; DNA/RNA_pol_sf.
DR   InterPro; IPR000605; Helicase_SF3_ssDNA/RNA_vir.
DR   InterPro; IPR014759; Helicase_SF3_ssRNA_vir.
DR   InterPro; IPR044067; PCV_3C_PRO.
DR   InterPro; IPR043128; Rev_trsase/Diguanyl_cyclase.
DR   InterPro; IPR001205; RNA-dir_pol_C.
DR   InterPro; IPR007094; RNA-dir_pol_PSvirus.
DR   Pfam; PF00680; RdRP_1; 1.
DR   Pfam; PF00910; RNA_helicase; 1.
DR   SUPFAM; SSF56672; DNA/RNA polymerases; 1.
DR   PROSITE; PS51874; PCV_3C_PRO; 1.
DR   PROSITE; PS50507; RDRP_SSRNA_POS; 1.
DR   PROSITE; PS51218; SF3_HELICASE_2; 1.
PE   3: Inferred from homology;
KW   ATP-binding; Covalent protein-RNA linkage; Helicase;
KW   Host endoplasmic reticulum; Host membrane; Hydrolase; Membrane;
KW   Nucleotide-binding; Nucleotidyltransferase; Protease; RNA-binding;
KW   RNA-directed RNA polymerase; Thiol protease; Transferase; Transmembrane;
KW   Transmembrane helix; Viral RNA replication.
FT   CHAIN           1..565
FT                   /note="P1A protein"
FT                   /evidence="ECO:0000255"
FT                   /id="PRO_0000037060"
FT   CHAIN           566..1203
FT                   /note="Putative ATP-dependent helicase"
FT                   /evidence="ECO:0000255"
FT                   /id="PRO_0000037061"
FT   CHAIN           1204..1230
FT                   /note="Viral genome-linked protein"
FT                   /evidence="ECO:0000250"
FT                   /id="PRO_0000037062"
FT   CHAIN           1231..1440
FT                   /note="Picornain 3C-like protease"
FT                   /evidence="ECO:0000255"
FT                   /id="PRO_0000037063"
FT   CHAIN           1441..2264
FT                   /note="RNA-directed RNA polymerase"
FT                   /evidence="ECO:0000255"
FT                   /id="PRO_0000037064"
FT   TOPO_DOM        566..1156
FT                   /note="Cytoplasmic"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        1157..1177
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TOPO_DOM        1178..1203
FT                   /note="Lumenal"
FT                   /evidence="ECO:0000255"
FT   DOMAIN          750..916
FT                   /note="SF3 helicase"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00551"
FT   DOMAIN          1227..1436
FT                   /note="Peptidase C3"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU01222"
FT   DOMAIN          1713..1841
FT                   /note="RdRp catalytic"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00539"
FT   ACT_SITE        1270
FT                   /note="For picornain 3C-like protease activity"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU01222"
FT   ACT_SITE        1308
FT                   /note="For picornain 3C-like protease activity"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU01222"
FT   ACT_SITE        1400
FT                   /note="For picornain 3C-like protease activity"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU01222"
FT   BINDING         780..787
FT                   /ligand="ATP"
FT                   /ligand_id="ChEBI:CHEBI:30616"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00551"
SQ   SEQUENCE   2264 AA;  253678 MW;  65949E4B5CE8B722 CRC64;
     MSVTLSPSGD CFSFNHVKYN NSLNKYLFYN SNLDIVLDDF DFYFNFYVKK YNVLLSFFSD
     RVLSALYTSM SVSEAASLAM EDFCELALDE LKINPFHQLW EETLANWPVY PGTSLLDFCR
     TQYEIRREAA EASAEILRLK EVARQRAFDD EVEFLIKHGA KVHFAPSFAA QLWRAGKDQK
     KCRGILLGKL NKAKALGEAH RSAVARAQAK AEVLREFEPS PQQIQRALEA QIFADRLSRK
     YAALTARVRA KRAAARELRE KELFLETQDL LNAPLLPPME KVGIERKYRK VRPTGSNVTS
     TPKPNVLENL CPFMGLGAKT ADVRCQATLM AGKIHPQYPR LASAIYAWVL GPSMKFECIA
     PVKTFIKGLT FMVDYFPEEV LIDELNKINS EARCFEASLV LEEERAKLEA HAENANCRAN
     IFMKAMAGVK NMAKCAYSGF LTGCEEAGRS LSEGVCSVMI NSFRECIKMI HKELGCAMEL
     IEVMIKKVKD WYNSMLEKLH CGLATLGTYA MYALAILLGC GLTTLLERCI GGAGILTKLF
     VTGVFAAIGL HAAGGFDGLQ REMVQMCTAL AAGIFDVHHK GNGKYSPMAD ILAEQRLEDR
     RADNVRSIPI ISGIISAMQQ FGTGLCSMHS ISLIEIGKLG AACHSMRMGK EALKEFCATI
     MYYLGRISDK VTGRETVFFD ELSTLVSVDV RGWILCAQSC IRESFHTEIG NQFFRDMVAQ
     LVDDGQKLQV GVNGIPRKIS TDYSQLSSDT EGPNELHKRT IRAGISEGRR CEPVWIYLFG
     QRHCGKSNFM ATLDNALAKH FGLPNTTAYR NCKDSFFSGY SGQTFFHVDD LSSVKLDPPM
     EAEMINLVSC QEVPLNMADL ADKPIYFRSP FIISSSNFED VPAGCGVRDI EAYRSRKACL
     VEMRRKPGVL FDPDNPLLAS QARFKDPMSQ MLLEGQTEEN SWLEMEDVVT EIINISARHR
     AAQEKLQARY MREKSLLDPL ALAAENFLKG EVQTHILIFL VLNLKSWNPK PQGGRGLYVD
     GSLYLLDPTF QFEEIPITDD GYKRLWDERM RKSFLSKIQT GEYLNSKSMV VTGFLRSLVN
     GDCAVLSKDT LSSSASVAQQ SIFKALGIDE RIYLRTLQHQ LDLYSADIPE NPYSNSAWIK
     ILKAIGMGRT YLAENGCGIL MIAAALILIL VSAWGFWKLF IGLFSGSMSL GAAIVGMSAV
     DIKAQQKSSS QEGGYRARNI PIHHRYAYAK SQAGDGLLPA ARFVCCYLST GGGFVSAMQY
     KNKSVRMTRH QALRFQEGEQ LTVIFSSTGE SQLIRWHKYM MREEPGSEIV TWLAPSLPSL
     SPDLKDLFLE DKEVDLPNHF KTIGYVLRVD NTAFHYDLLD TYAAVDKTPL PLKGVVGNEL
     YLHEIPEKIT FHYESRNDDC GMIILCQIKG KMRVVGMLVA GKDKTSWADI MPPNTLAELQ
     SQIEYIPKFG EAYDGFFKAG YVPMADAPTL PKKTNMVPVP QSLRVPCDVP IKEPAVLTNA
     DKRCPAGVNP PVTALKKKFE HPMKELEQEI LDEVATDILE TWYDCEDHVL NDIPLVVAIN
     GIPADSEEAE LENFVMKTSP GYPYFKNNRA EKLKGKSAYF EEAEDGTLKL KEGGMAAKLH
     ENLVEFTKNE VPELVVIECT KDELLPERKI KVGACRLFEI MPLHYNLFLR QKTCAFTQFL
     QHNRHVLPCQ VGTNPYSREW GHMLNRLMRP KTNEAINCDY SGFDGLLNAQ VIECIAKMIN
     RLYALSGESE VQQAQRYNML MALVGRYAFV GPEVYKVNCG LPSGFALTVV VNSVFNEILI
     RYAYKKLAPK PERNRFNQVV CLLVYGDDNL ISVSPSIASW FTGEAIRITL AEKKVKITDG
     SDKDAPTIEA KSFWELDFLK RKFLKLDNGI VQAPLDRSAI FSSLYWLTPD KSKFHASQRA
     SDFQGTVDVV EELILNVNVA LMELYLHNDP REFSRVRDFY IKALPLATGQ FRTWAFCEAF
     HSAQQTGMLK YDPAKVLDHM SGLDFKKFMH VSEQGNKAHF YTEMLGVAGP HYKPQENDFI
     VSTEPLKMGV CGEHVPIQYG SGVGGLPTKK WVLDFGRPSQ LKNKLGYLIH PILRAQIEAG
     KRLVFMSPAP YVANNAALIA FGTGGKMLIQ KDALVHYRNV IPESTSGLEQ YFDAPLPTAT
     IGTFYFANGE TYAALCEYKE GKVLNYEGFP TLILNEAAKD RKVPCMVATQ AKTKFKVSLA
     CDSTMCPHHT AVCETYEKAF RHCWLAKCKT SAVKVSPWHG TKLS
//