ExPASy logo ExPASy Home page Site Map Search ExPASy Contact us Swiss-Prot
Notice: This page will be replaced with www.uniprot.org. Please send us your feedback!
Search for

UniProtKB/Swiss-Prot entry P18294

[Entry info] [Name and origin] [References] [Comments] [Cross-references] [Keywords] [Features] [Sequence] [Tools]

Note: most headings are clickable, even if they don't appear as links. They link to the user manual or other documents.
Entry information
Entry name KCRF_STRPU
Primary accession number P18294
Secondary accession numbers None
Integrated into Swiss-Prot on November 1, 1990
Sequence was last modified on November 1, 1990 (Sequence version 1)
Annotations were last modified on    July 22, 2008 (Entry version 52)
Name and origin of the protein
Protein name Creatine kinase, flagellar
Synonym EC 2.7.3.2
Gene name None
From
Strongylocentrotus purpuratus (Purple sea urchin) [TaxID: 7668] 
Taxonomy Eukaryota; Metazoa; Echinodermata; Eleutherozoa; Echinozoa; Echinoidea; Euechinoidea; Echinacea; Echinoida; Strongylocentrotidae; Strongylocentrotus.
Protein existence 1: Evidence at protein level;
References
[1]
 NUCLEOTIDE SEQUENCE [MRNA], AND PARTIAL PROTEIN SEQUENCE.
TISSUE=Testis;
PubMed=2367531 [NCBI, ExPASy, EBI, Israel, Japan]
Wothe D.D., Charbonneau H., Shapiro B.M.;
"The phosphocreatine shuttle of sea urchin sperm: flagellar creatine kinase resulted from a gene triplication.";
Proc. Natl. Acad. Sci. U.S.A. 87:5203-5207(1990).
Comments
  • FUNCTION: This axonemal protein participates in an energy shuttle that utilizes phosphocreatine to transfer the energy from ATP generated by the mitochondrion in the sperm head to dynein in the distal portions of the flagellum.
  • CATALYTIC ACTIVITY: ATP + creatine = ADP + phosphocreatine.
  • SUBUNIT: Monomer.
  • SUBCELLULAR LOCATION: Cell projection, cilium, flagellum axoneme. Note=Associates specifically with the axoneme and may bind directly to polymerized microtubules.
  • DOMAIN: Contains three complete but non-identical creatine kinase segments flanked by unique regions.
  • SIMILARITY: Belongs to the ATP:guanido phosphotransferase family.
Copyright
Copyrighted by the UniProt Consortium, see http://www.uniprot.org/terms. Distributed under the Creative Commons Attribution-NoDerivs License.
Cross-references
Sequence databases
EMBL
M33763; AAA30049.1; -; mRNA.[EMBL / GenBank / DDBJ] [CoDingSequence]
PIR A43736; A43736.
RefSeq NP_999687.1; -.
UniGene Spu.13
3D structure databases
HSSP P00563; 2CRK. [HSSP ENTRY / PDB]
SMR P18294; 431-794.
ModBase P18294.
Ontologies
GO
GO:0004111; Molecular function: creatine kinase activity (inferred from electronic annotation from EC).
QuickGo view.
Family and domain databases
InterPro IPR000749; ATP-gua_Ptrans.
IPR014746; Gln_synth/guanido_kin_cat.
Graphical view of domain structure.
Gene3D G3DSA:1.10.135.10; ATP-gua_Ptrans; 3.
G3DSA:3.30.590.10; ATP-gua_Ptrans; 3.
PANTHER PTHR11547; ATP-gua_Ptrans; 1.
Pfam PF00217; ATP-gua_Ptrans; 3.
PF02807; ATP-gua_PtransN; 3.
Pfam graphical view of domain structure.
PROSITE PS00112; GUANIDO_KINASE; 3.
BLOCKS P18294.
Genome annotation databases
GeneID 373287; -.
KEGG spu:373287; -.
Other
ProtoNet P18294.
UniRef View cluster of proteins with at least 50% / 90% / 100% identity.
Keywords
ATP-binding; Cell projection; Cilium; Cilium biogenesis/degradation; Direct protein sequencing; Flagellum; Kinase; Nucleotide-binding; Repeat; Transferase.
Features
SEVIEWER logo Feature table viewer FT aligner logo Feature aligner
KeyFrom    To Length Description FTId
CHAIN   1   1174  1174     Creatine kinase, flagellar. PRO_0000212010
REPEAT   61    414  354     1; approximate. 
REPEAT   434    787  354     2; approximate. 
REPEAT   808   1161  354     3; approximate. 
NP_BIND   542    546  5     ATP (By similarity). 
NP_BIND   734    739  6     ATP (By similarity). 
ACT_SITE   324    324        By similarity. 
ACT_SITE   697    697        By similarity. 
ACT_SITE   1071   1071        By similarity. 
BINDING   605    605        ATP (By similarity). 
BINDING   706    706        ATP (By similarity). 
BINDING   749    749        ATP (By similarity). 
Sequence information
Length: 1174 AA [This is the length of the unprocessed precursor] Molecular weight: 130869 Da [This is the MW of the unprocessed precursor] CRC64: B8F4FBA5AC48EE93 [This is a checksum on the sequence] 
        10         20         30         40         50         60 
MGCAASSQQT TATGGQPAAG EKANPAPANN NPNAANKAET TGAAEELTKE SEPFVEPDPN 

        70         80         90        100        110        120 
YPDLSKHNNY LAESLTPSIY NKICNLRTLS GYSVDGCMQT GVDNPGHPFI KTVGLVAGDE 

       130        140        150        160        170        180 
ECYDLFADLF DPTIDKRHNG YPRNAKHTTD LNPDHLKGGD DFDPKYVLSC RVRTGRCIRG 

       190        200        210        220        230        240 
YGLPPHCTRA ERRDVEKVCK DALATLDGPL KGTYYPLTGM TEEMQDKLIA DHFLFDKPVS 

       250        260        270        280        290        300 
PLLMSARMAR DWPDGRGIWH NADKNFLVWI NEEDHTRVIS METSGNMKNV FKRFCNGLNK 

       310        320        330        340        350        360 
VENALKAKGY EFSWNEHLGY VLTCPSNLGT GVRAGVHIKI PLFSKHAGFE SILKHYRLQK 

       370        380        390        400        410        420 
RGTGGVDTAS TDGTFDISNL DRLGTSEVQQ VQSVVDGVKK LIELEKALEK GSDISGQIPR 

       430        440        450        460        470        480 
DPAIVRAEQV KEGYPDLSKH NNHLAHCLTY DIWKSLKDKK TPSGFTLDGC IQTGVMNPGH 

       490        500        510        520        530        540 
PHIMTVGMVA GDEESYDVFA DIFDPVIDAR HGGYPKDAVH VTNINHADLK GGDNLDPKYV 

       550        560        570        580        590        600 
LSCRVRTGRS IIGYSLPPHC TVEERAAVET ITIGALDKFD GDLQGKYYPL EGMSDETQTQ 

       610        620        630        640        650        660 
LIDDHFLFDK PVSPLLTAAR MHRDWPQGRG IWHNENKNFL VWVNEEDHIR VISMEKDGNM 

       670        680        690        700        710        720 
RAVFKRFCEG LQKFEQMIKK DGKEFMWNKH LGYVLTCPSN LGTGLRAGVH VKLPLLSKYP 

       730        740        750        760        770        780 
RFDQILRALR LQKRGTGGVD TASTDGTFDI SNLDRLGSSE VQQVQFVVDG VELLVQMEKK 

       790        800        810        820        830        840 
LEKGEDIFDI LPQQCRPKPP IKPFSYDYPD FSLHNNWMSK CMTEEIYNKL CNLKTKGGVT 

       850        860        870        880        890        900 
LNDCIQTGID NPGHPYIMTV GLVAGDEECY EVFAPLFDPV ISARHGGYAL DAKHPTNLNA 

       910        920        930        940        950        960 
AELKGGDDLD PEFVLSCRVR TGRCIRGLAL PPCCTRAERA EVEKITTEAL STLSGPLKGK 

       970        980        990       1000       1010       1020 
YYPLTGMTDE EQEKLIEDHF LFDKPVSPLL LCANMARDWP QGRGIWHNDE KNFLVWVNEE 

      1030       1040       1050       1060       1070       1080 
DHTRVISMEK SGNMKRVFER FCDGLKKVED SIKSKGYQFM WNEHLGYVLT CPSNLGTGLR 

      1090       1100       1110       1120       1130       1140 
AGVHVKVPLL SQQKIFDSIL DHMRLQKRGT GGVDTASTDG TFDISNSDRI GFSEVHLVQQ 

      1150       1160       1170 
LVDGVKLLVN LEKALMKGED INSLLPEKLR EDSS
P18294 in FASTA format

View entry in original UniProtKB/Swiss-Prot format
View entry in raw text format (no links)
Report form for errors/updates in this UniProtKB/Swiss-Prot entry

BLAST logo BLAST submission on ExPASy/SIB
or at NCBI (USA) 		Tools Sequence analysis tools: ProtParam, ProtScale, Compute pI/Mw, PeptideMass, PeptideCutter, Dotlet (Java)
PROSITE logo ScanProsite, MotifScan SWISS-MODEL Submit a homology modeling request to SWISS-MODEL
NPSA logo NPSA Sequence analysis tools

ExPASy logo ExPASy Home page Site Map Search ExPASy Contact us Swiss-Prot
Hosted by ch flag SIB Switzerland Mirror sites: Australia Brazil Canada China Korea
Notice: This page will be replaced with www.uniprot.org. Please send us your feedback!