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UniProtKB/Swiss-Prot entry P17980


[Entry info] [Name and origin] [References] [Comments] [Cross-references] [Keywords] [Features] [Sequence] [Tools]

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Entry information
Entry name PRS6A_HUMAN
Primary accession number P17980
Secondary accession numbers Q3B757 Q3B865 Q53HU5 Q6GPG8 Q6IBS1 Q96HD3
Integrated into Swiss-Prot on November 1, 1990
Sequence was last modified on May 10, 2002 (Sequence version 3)
Annotations were last modified on    July 22, 2008 (Entry version 93)
Name and origin of the protein
Protein name 26S protease regulatory subunit 6A
Synonyms Proteasome 26S subunit ATPase 3
TAT-binding protein 1
TBP-1
Proteasome subunit P50
Gene name
Name: PSMC3
Synonyms: TBP1
From
Homo sapiens (Human) [TaxID: 9606] 
Taxonomy Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini; Catarrhini; Hominidae; Homo.
Protein existence 1: Evidence at protein level;
References
[1]
NUCLEOTIDE SEQUENCE [MRNA].
PubMed=8419915 [NCBI, ExPASy, EBI, Israel, Japan]
Ohana B., Moore P.A., Ruben S.M., Southgate C.D., Green M.R., Rosen C.A.;
"The type 1 human immunodeficiency virus Tat binding protein is a transcriptional activator belonging to an additional family of evolutionarily conserved genes.";
Proc. Natl. Acad. Sci. U.S.A. 90:138-142(1993).
[2]
NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
TISSUE=Kidney, Lung, and Peripheral nerve;
DOI=10.1101/gr.2596504; PubMed=15489334 [NCBI, ExPASy, EBI, Israel, Japan]
The MGC Project Team;
"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC).";
Genome Res. 14:2121-2127(2004).
[3]
NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 16-439.
TISSUE=Adipose tissue;
Suzuki Y., Sugano S., Totoki Y., Toyoda A., Takeda T., Sakaki Y., Tanaka A., Yokoyama S.;
Submitted (APR-2005) to the EMBL/GenBank/DDBJ databases.
[4]
NUCLEOTIDE SEQUENCE [MRNA] OF 36-439, AND INTERACTION WITH HIV-1 TAT.
DOI=10.1126/science.2194290; PubMed=2194290 [NCBI, ExPASy, EBI, Israel, Japan]
Nelbock P., Dillion P.J., Perkins A., Rosen C.A.;
"A cDNA for a protein that interacts with the human immunodeficiency virus Tat transactivator.";
Science 248:1650-1653(1990).
[5]
NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 36-439.
Ebert L., Schick M., Neubert P., Schatten R., Henze S., Korn B.;
"Cloning of human full open reading frames in Gateway(TM) system entry vector (pDONR201).";
Submitted (JUN-2004) to the EMBL/GenBank/DDBJ databases.
[6]
PROTEIN SEQUENCE OF 156-171, AND MASS SPECTROMETRY.
TISSUE=Brain, and Cajal-Retzius cell;
Lubec G., Afjehi-Sadat L.;
Submitted (MAR-2007) to UniProtKB.
[7]
PARTIAL PROTEIN SEQUENCE.
DOI=10.1074/jbc.271.6.3112; PubMed=8621709 [NCBI, ExPASy, EBI, Israel, Japan]
Demartino G.N., Proske R.J., Moomaw C.R., Strong A.A., Song X., Hisamatsu H., Tanaka K., Slaughter C.A.;
"Identification, purification, and characterization of a PA700-dependent activator of the proteasome.";
J. Biol. Chem. 271:3112-3118(1996).
[8]
INTERACTION WITH PAAF1.
DOI=10.1128/MCB.25.9.3842-3853.2005; PubMed=15831487 [NCBI, ExPASy, EBI, Israel, Japan]
Park Y., Hwang Y.-P., Lee J.-S., Seo S.-H., Yoon S.K., Yoon J.-B.;
"Proteasomal ATPase-associated factor 1 negatively regulates proteasome activity by interacting with proteasomal ATPases.";
Mol. Cell. Biol. 25:3842-3853(2005).
[9]
PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-9, ACETYLATION AT MET-1, AND MASS SPECTROMETRY.
DOI=10.1021/bi061994u; PubMed=17323924 [NCBI, ExPASy, EBI, Israel, Japan]
Wang X., Chen C.-F., Baker P.R., Chen P.-L., Kaiser P., Huang L.;
"Mass spectrometric characterization of the affinity-purified human 26S proteasome complex.";
Biochemistry 46:3553-3565(2007).
Comments
Copyright
Copyrighted by the UniProt Consortium, see http://www.uniprot.org/terms. Distributed under the Creative Commons Attribution-NoDerivs License.
Cross-references
Sequence databases
EMBL
M34079; AAA36666.1; -; mRNA.[EMBL / GenBank / DDBJ] [CoDingSequence]
BC008713; AAH08713.4; -; mRNA.[EMBL / GenBank / DDBJ] [CoDingSequence]
BC073165; AAH73165.3; -; mRNA.[EMBL / GenBank / DDBJ] [CoDingSequence]
BC106920; AAI06921.1; -; mRNA.[EMBL / GenBank / DDBJ] [CoDingSequence]
BC107804; AAI07805.1; ALT_INIT; mRNA.[EMBL / GenBank / DDBJ] [CoDingSequence]
AK222485; BAD96205.1; -; mRNA.[EMBL / GenBank / DDBJ] [CoDingSequence]
CR456731; CAG33012.1; -; mRNA.[EMBL / GenBank / DDBJ] [CoDingSequence]
PIR A34832; A34832.
RefSeq NP_002795.2; -.
UniGene Hs.250758
3D structure databases
HSSP Q9LCZ4; 1IY0. [HSSP ENTRY / PDB]
ModBase P17980.
Protein-protein interaction databases
DIP DIP:27555N; -.
IntAct P17980; -.
PTM databases
PhosphoSite P17980; -.
Enzyme and pathway databases
Reactome REACT_11045; Signaling by Wnt.
REACT_152; Cell Cycle, Mitotic.
REACT_1538; Cell Cycle Checkpoints.
REACT_383; DNA Replication.
REACT_6185; HIV Infection.
REACT_6850; Cdc20:Phospho-APC/C mediated degradation of Cyclin A.
REACT_9035; APC/C:Cdh1-mediated degradation of Skp2.
2D gel databases
REPRODUCTION-2DPAGE IPI00018398; -.
Organism-specific databases
H-InvDB HIX0035958; -.
HGNC HGNC:9549; PSMC3.
GenAtlas PSMC3.
HPA HPA006065; -.
MIM 186852; gene. [NCBI / EBI]
PharmGKB PA33894; -.
GeneCards P17980.
Gene expression databases
ArrayExpress P17980; -.
CleanEx HS_PSMC3; -.
GermOnline ENSG00000165916; Homo sapiens.
Ontologies
GO
GO:0005634; Cellular component: nucleus (traceable author statement from ProtInc).
GO:0000502; Cellular component: proteasome complex (traceable author statement from ProtInc).
GO:0003713; Molecular function: transcription coactivator activity (traceable author statement from ProtInc).
GO:0003714; Molecular function: transcription corepressor activity (traceable author statement from ProtInc).
GO:0031145; Biological process: anaphase-promoting complex-dependent proteasomal ubiquitin-dependent protein catabolic process (inferred from experiment from Reactome).
GO:0051436; Biological process: negative regulation of ubiquitin-protein ligase activity during mitotic cell cycle (inferred from experiment from Reactome).
GO:0051437; Biological process: positive regulation of ubiquitin-protein ligase activity during mitotic cell cycle (inferred from experiment from Reactome).
QuickGo view.
Family and domain databases
InterPro IPR005937; 26S_Psome_P45.
IPR003593; AAA+_ATPase_core.
IPR003959; AAA_ATPase_core.
IPR003960; AAA_ATPase_CS.
Graphical view of domain structure.
Pfam PF00004; AAA; 1.
Pfam graphical view of domain structure.
SMART SM00382; AAA; 1.
SMART graphical view of domain structure.
TIGRFAMs TIGR01242; 26Sp45; 1.
PROSITE PS00674; AAA; 1.
BLOCKS P17980.
Proteomic databases
PeptideAtlas P17980; -.
Genome annotation databases
Ensembl ENSG00000165916; Homo sapiens. [Contig view]
GeneID 5702; -.
KEGG hsa:5702; -.
NMPDR fig|9606.3.peg.5546; -.
Phylogenomic databases
HOVERGEN P17980; -.
Other
SOURCE PSMC3; Homo sapiens.
ProtoNet P17980.
UniRef View cluster of proteins with at least 50% / 90% / 100% identity.
Keywords
Acetylation; ATP-binding; Cytoplasm; Direct protein sequencing; Host-virus interaction; Nucleotide-binding; Nucleus; Phosphoprotein; Proteasome.
Features
SEVIEWER logo Feature table viewer
KeyFrom   To Length Description FTId
CHAIN   1   439  439     26S protease regulatory subunit 6A. PRO_0000084698
NP_BIND   227   234  8     ATP (Potential). 
MOD_RES   1     1        N-acetylmethionine. 
MOD_RES   9     9        Phosphoserine. 
MUTAGEN   233   233        K->H: Loss of function. 
MUTAGEN   289   289        D->A: Loss of function. 
CONFLICT   356   356        M -> T (in Ref. 3; BAD96205). 
CONFLICT   409   409        R -> A (in Ref. 1; AAA36666 and 4). 
Sequence information
Length: 439 AA [This is the length of the unprocessed precursor] Molecular weight: 49204 Da [This is the MW of the unprocessed precursor] CRC64: 0E443465DDDEBB0B [This is a checksum on the sequence]
        10         20         30         40         50         60 
MNLLPNIESP VTRQEKMATV WDEAEQDGIG EEVLKMSTEE IIQRTRLLDS EIKIMKSEVL 

        70         80         90        100        110        120 
RVTHELQAMK DKIKENSEKI KVNKTLPYLV SNVIELLDVD PNDQEEDGAN IDLDSQRKGK 

       130        140        150        160        170        180 
CAVIKTSTRQ TYFLPVIGLV DAEKLKPGDL VGVNKDSYLI LETLPTEYDS RVKAMEVDER 

       190        200        210        220        230        240 
PTEQYSDIGG LDKQIQELVE AIVLPMNHKE KFENLGIQPP KGVLMYGPPG TGKTLLARAC 

       250        260        270        280        290        300 
AAQTKATFLK LAGPQLVQMF IGDGAKLVRD AFALAKEKAP SIIFIDELDA IGTKRFDSEK 

       310        320        330        340        350        360 
AGDREVQRTM LELLNQLDGF QPNTQVKVIA ATNRVDILDP ALLRSGRLDR KIEFPMPNEE 

       370        380        390        400        410        420 
ARARIMQIHS RKMNVSPDVN YEELARCTDD FNGAQCKAVC VEAGMIALRR GATELTHEDY 

       430 
MEGILEVQAK KKANLQYYA 

P17980 in FASTA format

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