[1]	NUCLEOTIDE SEQUENCE [MRNA]. TISSUE=Brain; DOI=10.1093/nar/18.12.3638; PubMed=2362815 [NCBI, ExPASy, EBI, Israel, Japan] Kaghad M., Dumont X., Chalon P., Lelias J.M., Lamande N., Lucas M., Lazar M., Caput D.; "Nucleotide sequences of cDNAs alpha and gamma enolase mRNAs from mouse brain."; Nucleic Acids Res. 18:3638-3638(1990).
[2]	NUCLEOTIDE SEQUENCE [GENOMIC DNA]. PubMed=9445485 [NCBI, ExPASy, EBI, Israel, Japan] Ansari-Lari M.A., Oeltjen J.C., Schwartz S., Zhang Z., Muzny D.M., Lu J., Gorrell J.H., Chinault A.C., Belmont J.W., Miller W., Gibbs R.A.; "Comparative sequence analysis of a gene-rich cluster at human chromosome 12p13 and its syntenic region in mouse chromosome 6."; Genome Res. 8:29-40(1998).
[3]	NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. TISSUE=Retina; DOI=10.1101/gr.2596504; PubMed=15489334 [NCBI, ExPASy, EBI, Israel, Japan] The MGC Project Team; "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."; Genome Res. 14:2121-2127(2004).
[4]	PROTEIN SEQUENCE OF 16-28; 33-50; 73-89; 104-120; 163-179; 184-193; 203-228; 240-262; 270-285; 307-326; 336-358; 373-394 AND 413-422, AND MASS SPECTROMETRY. STRAIN=C57BL/6; TISSUE=Brain; Lubec G., Kang S.U.; Submitted (APR-2007) to UniProtKB.
[5]	PROTEIN SEQUENCE OF 33-50; 73-89; 163-179; 270-285 AND 423-429, AND MASS SPECTROMETRY. TISSUE=Hippocampus; Lubec G., Klug S.; Submitted (MAR-2007) to UniProtKB.
[6]	PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-80, AND MASS SPECTROMETRY. TISSUE=Brain; DOI=10.1002/pmic.200401066; PubMed=15648052 [NCBI, ExPASy, EBI, Israel, Japan] Vosseller K., Hansen K.C., Chalkley R.J., Trinidad J.C., Wells L., Hart G.W., Burlingame A.L.; "Quantitative analysis of both protein expression and serine / threonine post-translational modifications through stable isotope labeling with dithiothreitol."; Proteomics 5:388-398(2005).
[7]	PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT TYR-25 AND TYR-44, AND MASS SPECTROMETRY. TISSUE=Mast cell; PubMed=17947660 [NCBI, ExPASy, EBI, Israel, Japan] Cao L., Yu K., Banh C., Nguyen V., Ritz A., Raphael B.J., Kawakami Y., Kawakami T., Salomon A.R.; "Quantitative time-resolved phosphoproteomic analysis of mast cell signaling."; J. Immunol. 179:5864-5876(2007).
[8]	PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT TYR-25 AND TYR-44, AND MASS SPECTROMETRY. TISSUE=Brain; DOI=10.1021/pr0701254; PubMed=18034455 [NCBI, ExPASy, EBI, Israel, Japan] Ballif B.A., Carey G.R., Sunyaev S.R., Gygi S.P.; "Large-scale identification and evolution indexing of tyrosine phosphorylation sites from murine brain."; J. Proteome Res. 7:311-318(2008).

Comments

FUNCTION: Has neurotrophic and neuroprotective properties on a broad spectrum of central nervous system (CNS) neurons. Binds, in a calcium-dependent manner, to cultured neocortical neurons and promotes cell survival (By similarity).
CATALYTIC ACTIVITY: 2-phospho-D-glycerate = phosphoenolpyruvate + H₂O.
COFACTOR: Magnesium. Required for catalysis and for stabilizing the dimer.
PATHWAY: Carbohydrate degradation; glycolysis; pyruvate from D-glyceraldehyde 3-phosphate: step 4/5 .
SUBUNIT: Mammalian enolase is composed of 3 isozyme subunits, alpha, beta and gamma, which can form homodimers or heterodimers which are cell-type and development-specific.
SUBCELLULAR LOCATION: Cytoplasm (By similarity). Cell membrane (By similarity). Note=Can translocate to the plasma membrane in either the homodimeric (alpha/alpha) or heterodimeric (alpha/gamma) form (By similarity).
TISSUE SPECIFICITY: The alpha/alpha homodimer is expressed in embryo and in most adult tissues. The alpha/beta heterodimer and the beta/beta homodimer are found in striated muscle, and the alpha/gamma heterodimer and the gamma/gamma homodimer in neurons.
DEVELOPMENTAL STAGE: During ontogenesis, there is a transition from the alpha/alpha homodimer to the alpha/beta heterodimer in striated muscle cells, and to the alpha/gamma heterodimer in nerve cells.
SIMILARITY: Belongs to the enolase family.

Copyright

Copyrighted by the UniProt Consortium, see http://www.uniprot.org/terms. Distributed under the Creative Commons Attribution-NoDerivs License.

Cross-references

Sequence databases

EMBL

X52380; CAA36606.1; -; mRNA.	[EMBL / GenBank / DDBJ] [CoDingSequence]
AC002397; AAC36002.1; -; Genomic_DNA.	[EMBL / GenBank / DDBJ] [CoDingSequence]
BC031739; AAH31739.1; -; mRNA.	[EMBL / GenBank / DDBJ] [CoDingSequence]

S10247; S10247.

NP_038537.1; -.

3D structure databases

HSSP

P56252; 1PDZ. [HSSP ENTRY / PDB]

P17183; 2-434.

PTM databases

P17183; -.

Organism-specific databases

MGI

MGI:95394; Eno2.

Gene expression databases

ArrayExpress

P17183; -.

CleanEx

MM_ENO2; -.

GermOnline

ENSMUSG00000004267; Mus musculus.

Ontologies

GO:0005886;	Cellular component: plasma membrane (inferred from electronic annotation from UniProtKB-SubCell).
QuickGo view.

Family and domain databases

InterPro

IPR000941; Enolase.
Graphical view of domain structure.

PANTHER

PTHR11902; Enolase; 1.

Pfam

PF00113; Enolase_C; 1.
PF03952; Enolase_N; 1.
Pfam graphical view of domain structure.

PIRSF

PIRSF001400; Enolase; 1.

PRINTS

PR00148; ENOLASE.

ProDom

PD000902; Enolase; 1.
[Domain structure / List of seq. sharing at least 1 domain]

TIGR01060; eno; 1.

PS00164; ENOLASE; 1.

Genome annotation databases

Ensembl

ENSMUSG00000004267; Mus musculus. [Contig view]

GeneID

13807; -.

KEGG

mmu:13807; -.

Phylogenomic databases

HOGENOM

P17183; -.

HOVERGEN

P17183; -.

Other

Eno2; Mus musculus.

View cluster of proteins with at least 50% / 90% / 100% identity.

Keywords

Cell membrane; Cytoplasm; Direct protein sequencing; Glycolysis; Lyase; Magnesium; Membrane; Metal-binding; Phosphoprotein.

Features

Feature table viewer

Feature aligner

`Key`	`From To`	`Length`	`Description`	`FTId`
`INIT_MET`	`1 1`		`Removed (By similarity).`
`CHAIN`	`2 434`	`433`	`Gamma-enolase.`	`PRO_0000134113`
`REGION`	`370 373`	`4`	`Substrate binding (By similarity).`
`ACT_SITE`	`210 210`		`Proton donor (By similarity).`
`ACT_SITE`	`343 343`		`Proton acceptor (By similarity).`
`METAL`	`245 245`		`Magnesium (By similarity).`
`METAL`	`293 293`		`Magnesium (By similarity).`
`METAL`	`318 318`		`Magnesium (By similarity).`
`BINDING`	`158 158`		`Substrate (By similarity).`
`BINDING`	`167 167`		`Substrate (By similarity).`
`BINDING`	`293 293`		`Substrate (By similarity).`
`BINDING`	`318 318`		`Substrate (By similarity).`
`BINDING`	`394 394`		`Substrate (By similarity).`
`MOD_RES`	`25 25`		`Phosphotyrosine.`
`MOD_RES`	`44 44`		`Phosphotyrosine.`
`MOD_RES`	`80 80`		`Phosphoserine.`

Sequence information

Length: 434 AA [This is the length of the unprocessed precursor]

Molecular weight: 47297 Da [This is the MW of the unprocessed precursor]

CRC64: A5C7F189E913392E [This is a checksum on the sequence]

        10         20         30         40         50         60 
MSIEKIWARE ILDSRGNPTV EVDLYTAKGL FRAAVPSGAS TGIYEALELR DGDKQRYLGK 

        70         80         90        100        110        120 
GVLKAVDHIN SRIAPALISS GISVVEQEKL DNLMLELDGT ENKSKFGANA ILGVSLAVCK 

       130        140        150        160        170        180 
AGAAERDLPL YRHIAQLAGN SDLILPVPAF NVINGGSHAG NKLAMQEFMI LPVGAESFRD 

       190        200        210        220        230        240 
AMRLGAEVYH TLKGVIKDKY GKDATNVGDE GGFAPNILEN SEALELVKEA IDKAGYTEKM 

       250        260        270        280        290        300 
VIGMDVAASE FYRDGKYDLD FKSPADPSRY ITGDQLGALY QDFVRNYPVV SIEDPFDQDD 

       310        320        330        340        350        360 
WAAWSKFTAN VGIQIVGDDL TVTNPKRIER AVEEKACNCL LLKVNQIGSV TEAIQACKLA 

       370        380        390        400        410        420 
QENGWGVMVS HRSGETEDTF IADLVVGLCT GQIKTGAPCR SERLAKYNQL MRIEEELGDE 

       430 
ARFAGHNFRN PSVL

P17183 in FASTA format

View entry in original UniProtKB/Swiss-Prot format
View entry in raw text format (no links)
Report form for errors/updates in this UniProtKB/Swiss-Prot entry

	BLAST submission on ExPASy/SIB or at NCBI (USA)		Sequence analysis tools: ProtParam, ProtScale, Compute pI/Mw, PeptideMass, PeptideCutter, Dotlet (Java)
	ScanProsite, MotifScan		Submit a homology modeling request to SWISS-MODEL
	NPSA Sequence analysis tools