RNA-directed RNA polymerase
     (EC 2.7.7.48)
mRNA (guanine-N(7)-)-methyltransferase
     (EC 2.1.1.56)
mRNA guanylyltransferase
     (EC 2.7.7.-)

Gene name

Name:

From

Rabies virus (strain SAD B19) (RABV)

[TaxID: 11300]

Taxonomy

Viruses; ssRNA negative-strand viruses; Mononegavirales; Rhabdoviridae; Lyssavirus.

Virus host

Mammalia [TaxID: 40674]

Protein existence

1: Evidence at protein level;

References

[1]	NUCLEOTIDE SEQUENCE [GENOMIC RNA]. DOI=10.1016/0042-6822(90)90433-R; PubMed=2139267 [NCBI, ExPASy, EBI, Israel, Japan] Conzelmann K.-K., Cox J.H., Schneider L.G., Thiel H.-J.; "Molecular cloning and complete nucleotide sequence of the attenuated rabies virus SAD B19."; Virology 175:485-499(1990).
[2]	NUCLEOTIDE SEQUENCE [GENOMIC RNA]. STRAIN=SRV9; Wang T., Zhang S., Hu R.; "Analysis of the whole sequence of rabies virus vaccine strain SRV9."; Submitted (APR-2004) to the EMBL/GenBank/DDBJ databases.
[3]	NUCLEOTIDE SEQUENCE [GENOMIC RNA]. STRAIN=Isolate SAD Bern, Isolate SAD Bern original var 1, Isolate SAD Bern original var 2, Isolate SAD Bern original var 3, Isolate SAD Bern original var 4, Isolate SAD Bern original var 5, Isolate SAD P5/88, Isolate SAD VA1, Isolate SAD1-3670 var 1, Isolate SAD1-3670 var 2, and Isolate SAG 2; Geue L., Schares S., Schnick C., Kliemt J., Beckert A., Hoffmann B., Freuling C., Marston D., McElhinney L., Fooks A., Zanoni R., Peterhans E., Cox J., Mueller T.; "Complete nucleotide sequencing of SAD derivatives of attenuated rabies virus vaccine strains."; Submitted (JAN-2007) to the EMBL/GenBank/DDBJ databases.
[4]	MUTAGENESIS OF LEU-725; ALA-726; GLN-727; GLY-728; ASP-729; ASN-730; GLN-731; VAL-732 AND LEU-733. DOI=10.1006/viro.1995.0063; PubMed=8553554 [NCBI, ExPASy, EBI, Israel, Japan] Schnell M.J., Conzelmann K.K.; "Polymerase activity of in vitro mutated rabies virus L protein."; Virology 214:522-530(1995).

Comments

FUNCTION: Displays RNA-directed RNA polymerase, mRNA guanylyl transferase, mRNA (guanine-7-N-)-methyltransferase and poly(A) synthetase activities. The viral mRNA guanylyl transferase displays a different biochemical reaction than the cellular enzyme. The template is composed of the viral RNA tightly encapsidated by the nucleoprotein (N). Functions either as transcriptase or as replicase. The transcriptase synthesizes subsequently five subgenomic RNAs, assuring their capping and polyadenylation by a stuttering mechanism. The replicase mode is dependent on intracellular N protein concentration. In this mode, the polymerase replicates the whole viral genome without recognizing the transcriptional signals (By similarity).
CATALYTIC ACTIVITY: Nucleoside triphosphate + RNA(n) = diphosphate + RNA(n+1).
CATALYTIC ACTIVITY: S-adenosyl-L-methionine + G(5')pppR-RNA = S-adenosyl-L-homocysteine + m₇G(5')pppR-RNA.
SUBUNIT: Interacts with the P protein to form the functional polymerase (By similarity).
SUBCELLULAR LOCATION: Virion. Cytoplasm (By similarity).
SIMILARITY: Belongs to the rhabdoviruses protein L family.
SIMILARITY: Contains 1 RdRp catalytic domain.

Copyright

Copyrighted by the UniProt Consortium, see http://www.uniprot.org/terms. Distributed under the Creative Commons Attribution-NoDerivs License.

Cross-references

Sequence databases

EMBL

M31046; AAA47203.1; -; Genomic_RNA.	[EMBL / GenBank / DDBJ] [CoDingSequence]
AF499686; AAT48626.1; -; Genomic_RNA.	[EMBL / GenBank / DDBJ] [CoDingSequence]
EF206708; ABN11300.1; -; Genomic_RNA.	[EMBL / GenBank / DDBJ] [CoDingSequence]
EF206709; ABN11305.1; -; Genomic_RNA.	[EMBL / GenBank / DDBJ] [CoDingSequence]
EF206710; ABN11310.1; -; Genomic_RNA.	[EMBL / GenBank / DDBJ] [CoDingSequence]
EF206711; ABN11315.1; -; Genomic_RNA.	[EMBL / GenBank / DDBJ] [CoDingSequence]
EF206712; ABN11320.1; -; Genomic_RNA.	[EMBL / GenBank / DDBJ] [CoDingSequence]
EF206713; ABN11325.1; -; Genomic_RNA.	[EMBL / GenBank / DDBJ] [CoDingSequence]
EF206714; ABN11330.1; -; Genomic_RNA.	[EMBL / GenBank / DDBJ] [CoDingSequence]
EF206715; ABN11335.1; -; Genomic_RNA.	[EMBL / GenBank / DDBJ] [CoDingSequence]
EF206716; ABN11340.1; -; Genomic_RNA.	[EMBL / GenBank / DDBJ] [CoDingSequence]
EF206717; ABN11345.1; -; Genomic_RNA.	[EMBL / GenBank / DDBJ] [CoDingSequence]
EF206718; ABN11350.1; -; Genomic_RNA.	[EMBL / GenBank / DDBJ] [CoDingSequence]
EF206719; ABN11355.1; -; Genomic_RNA.	[EMBL / GenBank / DDBJ] [CoDingSequence]
EF206720; ABN11360.1; -; Genomic_RNA.	[EMBL / GenBank / DDBJ] [CoDingSequence]

PIR

E34746; ZLVNSB.

3D structure databases

ModBase

P16289.

Family and domain databases

InterPro

IPR017234; RNA-dir_RNA_pol_rhabdovir.
IPR014023; RNA_pol_cat.
IPR001016; RNA_pol_L_viral.
Graphical view of domain structure.

Pfam

PF00946; Paramyx_RNA_pol; 1.
Pfam graphical view of domain structure.

PIRSF

PIRSF037546; RNA_pol_RhabdoV_sub; 1.

PROSITE

PS50526; RDRP_SSRNA_NEG_NONSEG; 1.
PROSITE graphical view of domain structure (profiles).

Other

View cluster of proteins with at least 50% / 90% / 100% identity.

Keywords

ATP-binding; Complete proteome; Cytoplasm; Methyltransferase; mRNA capping; mRNA processing; Multifunctional enzyme; Nucleotide-binding; Nucleotidyltransferase; RNA replication; RNA-directed RNA polymerase; S-adenosyl-L-methionine; Transferase; Virion.

Features

Feature table viewer

Feature aligner

`Key`	`From To`	`Length`	`Description`	`FTId`
`CHAIN`	`1 2127`	`2127`	`Large structural protein.`	`PRO_0000222841`
`DOMAIN`	`611 799`	`189`	`RdRp catalytic.`
`NP_BIND`	`1701 1710`	`10`	`ATP (Potential).`
`REGION`	`1562 2127`	`566`	`Interaction with P protein (By similarity).`
`VARIANT`	`26 26`	`1`	`I -> T (in strain: Isolate SAD1-3670 var 1 and Isolate SAD1-3670 var 2).`
`VARIANT`	`365 365`	`1`	`F -> Y (in strain: Isolate SAD1-3670 var 1 and Isolate SAD1-3670 var 2).`
`VARIANT`	`391 391`	`1`	`L -> I (in strain: Isolate SAD1-3670 var 1 and Isolate SAD1-3670 var 2).`
`VARIANT`	`394 394`	`1`	`M -> V (in strain: SRV9, Isolate SAD1-3670 var 1, Isolate SAD1-3670 var 2, Isolate SAD Bern, Isolate SAD Bern original var 1, Isolate SAD Bern original var 2, Isolate SAD Bern original var 3, Isolate SAD Bern original var 4, Isolate SAD Bern original var 5, Isolate SAD P5/88, Isolate SAD VA1 and Isolate SAG 2).`
`VARIANT`	`524 524`	`1`	`R -> K (in strain: Isolate SAD1-3670 var 1 and Isolate SAD1-3670 var 2).`
`VARIANT`	`901 901`	`1`	`I -> V (in strain: Isolate SAD1-3670 var 1 and Isolate SAD1-3670 var 2).`
`VARIANT`	`933 933`	`1`	`Q -> H (in strain: Isolate SAD1-3670 var 1 and Isolate SAD1-3670 var 2).`
`VARIANT`	`1018 1018`	`1`	`I -> T (in strain: Isolate SAD1-3670 var 1 and Isolate SAD1-3670 var 2).`
`VARIANT`	`1100 1100`	`1`	`L -> Q (in strain: SRV9).`
`VARIANT`	`1212 1212`	`1`	`A -> T (in strain: Isolate SAD1-3670 var 1 and Isolate SAD1-3670 var 2).`
`VARIANT`	`1450 1450`	`1`	`V -> I (in strain: Isolate SAD1-3670 var 1 and Isolate SAD1-3670 var 2).`
`VARIANT`	`1516 1516`	`1`	`I -> V (in strain: Isolate SAD1-3670 var 1 and Isolate SAD1-3670 var 2).`
`VARIANT`	`1541 1541`	`1`	`S -> T (in strain: Isolate SAD1-3670 var 1 and Isolate SAD1-3670 var 2).`
`VARIANT`	`1761 1761`	`1`	`L -> F (in strain: Isolate SAD1-3670 var 1 and Isolate SAD1-3670 var 2).`
`VARIANT`	`1990 1990`	`1`	`S -> L (in strain: Isolate SAD1-3670 var 1 and Isolate SAD1-3670 var 2).`
`VARIANT`	`2097 2097`	`1`	`R -> G (in strain: Isolate SAD1-3670 var 1 and Isolate SAD1-3670 var 2).`
`MUTAGEN`	`725 725`		`L->I,M: Complete loss of polymerase activity.`
`MUTAGEN`	`726 726`		`A->G,S,V: Complete loss of polymerase activity.`
`MUTAGEN`	`727 727`		`Q->E,N: Complete loss of polymerase activity.`
`MUTAGEN`	`728 730`		`GDN->SDD: Complete loss of polymerase activity.`
`MUTAGEN`	`728 728`		`G->A: Complete loss of polymerase activity.`
`MUTAGEN`	`729 729`		`D->E,N: Complete loss of polymerase activity.`
`MUTAGEN`	`730 730`		`N->A,D,E,Q: Complete loss of polymerase activity.`
`MUTAGEN`	`731 731`		`Q->E,N: Complete loss of polymerase activity.`
`MUTAGEN`	`732 732`		`V->A: Partial loss of polymerase activity.`
`MUTAGEN`	`732 732`		`V->S,T: Complete loss of polymerase activity.`
`MUTAGEN`	`733 733`		`L->I: No effect on polymerase activity.`
`MUTAGEN`	`733 733`		`L->N: Partial loss of polymerase activity.`
`MUTAGEN`	`733 733`		`L->S: Partial loss of polymerase activity.`

Sequence information

Length: 2127 AA [This is the length of the unprocessed precursor]

Molecular weight: 242979 Da [This is the MW of the unprocessed precursor]

CRC64: 6D74F0779CCBBDF9 [This is a checksum on the sequence]

        10         20         30         40         50         60 
MLDPGEVYDD PIDPIELEAE PRGTPIVPNI LRNSDYNLNS PLIEDPARLM LEWLKTGNRP 

        70         80         90        100        110        120 
YRMTLTDNCS RSFRVLKDYF KKVDLGSLKV GGMAAQSMIS LWLYGAHSES NRSRRCITDL 

       130        140        150        160        170        180 
AHFYSKSSPI EKLLNLTLGN RGLRIPPEGV LSCLERVDYD NAFGRYLANT YSSYLFFHVI 

       190        200        210        220        230        240 
TLYMNALDWD EEKTILALWK DLTSVDIGKD LVKFKDQIWG LLIVTKDFVY SQSSNCLFDR 

       250        260        270        280        290        300 
NYTLMLKDLF LSRFNSLMVL LSPPEPRYSD DLISQLCQLY IAGDQVLSMC GNSGYEVIKI 

       310        320        330        340        350        360 
LEPYVVNSLV QRAEKFRPLI HSLGDFPVFI KDKVSQLEET FGPCARRFFR ALDQFDNIHD 

       370        380        390        400        410        420 
LVFVFGCYRH WGHPYIDYRK GLSKLYDQVH LKKMIDKSYQ ECLASDLARR ILRWGFDKYS 

       430        440        450        460        470        480 
KWYLDSRFLA RDHPLTPYIK TQTWPPKHIV DLVGDTWHKL PITQIFEIPE SMDPSEILDD 

       490        500        510        520        530        540 
KSHSFTRTRL ASWLSENRGG PVPSEKVIIT ALSKPPVNPR EFLRSIDLGG LPDEDLIIGL 

       550        560        570        580        590        600 
KPKERELKIE GRFFALMSWN LRLYFVITEK LLANYILPLF DALTMTDNLN KVFKKLIDRV 

       610        620        630        640        650        660 
TGQGLLDYSR VTYAFHLDYE KWNNHQRLES TEDVFSVLDQ VFGLKRVFSR THEFFQKAWI 

       670        680        690        700        710        720 
YYSDRSDLIG LREDQIYCLD ASNGPTCWNG QDGGLEGLRQ KGWSLVSLLM IDRESQIRNT 

       730        740        750        760        770        780 
RTKILAQGDN QVLCPTYMLS PGLSQEGLLY ELERISRNAL SIYRAVEEGA SKLGLIIKKE 

       790        800        810        820        830        840 
ETMCSYDFLI YGKTPLFRGN ILVPESKRWA RVSCVSNDQI VNLANIMSTV STNALTVAQH 

       850        860        870        880        890        900 
SQSLIKPMRD FLLMSVQAVF HYLLFSPILK GRVYKILSAE GESFLLAMSR IIYLDPSLGG 

       910        920        930        940        950        960 
ISGMSLGRFH IRQFSDPVSE GLSFWREIWL SSQESWIHAL CQEAGNPDLG ERTLESFTRL 

       970        980        990       1000       1010       1020 
LEDPTTLNIR GGASPTILLK DAIRKALYDE VDKVENSEFR EAILLSKTHR DNFILFLISV 

      1030       1040       1050       1060       1070       1080 
EPLFPRFLSE LFSSSFLGIP ESIIGLIQNS RTIRRQFRKS LSKTLEESFY NSEIHGISRM 

      1090       1100       1110       1120       1130       1140 
TQTPQRVGGV WPCSSERADL LREISWGRKV VGTTVPHPSE MLGLLPKSSI SCTCGATGGG 

      1150       1160       1170       1180       1190       1200 
NPRVSVSVLP SFDQSFFSRG PLKGYLGSST SMSTQLFHAW EKVTNVHVVK RALSLKESIN 

      1210       1220       1230       1240       1250       1260 
WFITRDSNLA QALIRNIMSL TGPDFPLEEA PVFKRTGSAL HRFKSARYSE GGYSSVCPNL 

      1270       1280       1290       1300       1310       1320 
LSHISVSTDT MSDLTQDGKN YDFMFQPLML YAQTWTSELV QRDTRLRDST FHWHLRCNRC 

      1330       1340       1350       1360       1370       1380 
VRPIDDVTLE TSQIFEFPDV SKRISRMVSG AVPHFQRLPD IRLRPGDFES LSGREKSHHI 

      1390       1400       1410       1420       1430       1440 
GSAQGLLYSI LVAIHDSGYN DGTIFPVNIY GKVSPRDYLR GLARGVLIGS SICFLTRMTN 

      1450       1460       1470       1480       1490       1500 
ININRPLELV SGVISYILLR LDNHPSLYIM LREPSLRGEI FSIPQKIPAA YPTTMKEGNR 

      1510       1520       1530       1540       1550       1560 
SILCYLQHVL RYEREIITAS PENDWLWIFS DFRSAKMTYL SLITYQSHLL LQRVERNLSK 

      1570       1580       1590       1600       1610       1620 
SMRDNLRQLS SLMRQVLGGH GEDTLESDDN IQRLLKDSLR RTRWVDQEVR HAARTMTGDY 

      1630       1640       1650       1660       1670       1680 
SPNKKVSRKV GCSEWVCSAQ QVAVSTSANP APVSELDIRA LSKRFQNPLI SGLRVVQWAT 

      1690       1700       1710       1720       1730       1740 
GAHYKLKPIL DDLNVFPSLC LVVGDGSGGI SRAVLNMFPD AKLVFNSLLE VNDLMASGTH 

      1750       1760       1770       1780       1790       1800 
PLPPSAIMRG GNDIVSRVID LDSIWEKPSD LRNLATWKYF QSVQKQVNMS YDLIICDAEV 

      1810       1820       1830       1840       1850       1860 
TDIASINRIT LLMSDFALSI DGPLYLVFKT YGTMLVNPNY KAIQHLSRAF PSVTGFITQV 

      1870       1880       1890       1900       1910       1920 
TSSFSSELYL RFSKRGKFFR DAEYLTSSTL REMSLVLFNC SSPKSEMQRA RSLNYQDLVR 

      1930       1940       1950       1960       1970       1980 
GFPEEIISNP YNEMIITLID SDVESFLVHK MVDDLELQRG TLSKVAIIIA IMIVFSNRVF 

      1990       2000       2010       2020       2030       2040 
NVSKPLTDPS FYPPSDPKIL RHFNICCSTM MYLSTALGDV PSFARLHDLY NRPITYYFRK 

      2050       2060       2070       2080       2090       2100 
QVIRGNVYLS WSWSNDTSVF KRVACNSSLS LSSHWIRLIY KIVKTTRLVG SIKDLSREVE 

      2110       2120 
RHLHRYNRWI TLEDIRSRSS LLDYSCL

P16289 in FASTA format

View entry in original UniProtKB/Swiss-Prot format
View entry in raw text format (no links)
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	BLAST submission on ExPASy/SIB or at NCBI (USA)		Sequence analysis tools: ProtParam, ProtScale, Compute pI/Mw, PeptideMass, PeptideCutter, Dotlet (Java)
	ScanProsite, MotifScan		Submit a homology modeling request to SWISS-MODEL
	NPSA Sequence analysis tools