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UniProtKB/Swiss-Prot entry P15336


[Entry info] [Name and origin] [References] [Comments] [Cross-references] [Keywords] [Features] [Sequence] [Tools]

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Entry information
Entry name ATF2_HUMAN
Primary accession number P15336
Secondary accession numbers Q13000 Q4ZFU9 Q53RY2
Integrated into Swiss-Prot on April 1, 1990
Sequence was last modified on November 25, 2008 (Sequence version 4)
Annotations were last modified on    June 16, 2009 (Entry version 107)
Name and origin of the protein
Protein name Cyclic AMP-dependent transcription factor ATF-2
Synonyms cAMP-dependent transcription factor ATF-2
Activating transcription factor 2
cAMP response element-binding protein CRE-BP1
HB16
Cyclic AMP-responsive element-binding protein 2
cAMP-responsive element-binding protein 2
CREB-2
Gene name
Name: ATF2
Synonyms: CREB2, CREBP1
From
Homo sapiens (Human) [TaxID: 9606] 
Taxonomy Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini; Catarrhini; Hominidae; Homo.
Protein existence 1: Evidence at protein level;
References
[1]
NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1).
TISSUE=Fetal brain;
PubMed=2529117 [NCBI, ExPASy, EBI, Israel, Japan]
Maekawa T., Sakura H., Kanei-Ishii C., Sudo T., Yoshimura T., Fujisawa J., Yoshida M., Ishii S.;
"Leucine zipper structure of the protein CRE-BP1 binding to the cyclic AMP response element in brain.";
EMBO J. 8:2023-2028(1989).
[2]
NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 2).
TISSUE=Thymus;
PubMed=9058782 [NCBI, ExPASy, EBI, Israel, Japan]
Yang L., Lanier E.R., Kraig E.;
"Identification of a novel, spliced variant of CREB that is preferentially expressed in the thymus.";
J. Immunol. 158:2522-2525(1997).
[3]
NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
DOI=10.1038/nature03466; PubMed=15815621 [NCBI, ExPASy, EBI, Israel, Japan]
Hillier L.W., Graves T.A., Fulton R.S., Fulton L.A., Pepin K.H., Minx P., Wagner-McPherson C., Layman D., Wylie K., Sekhon M., Becker M.C., Fewell G.A., Delehaunty K.D., Miner T.L., Nash W.E., Kremitzki C., Oddy L., Du H., Sun H., Bradshaw-Cordum H., Ali J., Carter J., Cordes M., Harris A., Isak A., van Brunt A., Nguyen C., Du F., Courtney L., Kalicki J., Ozersky P., Abbott S., Armstrong J., Belter E.A., Caruso L., Cedroni M., Cotton M., Davidson T., Desai A., Elliott G., Erb T., Fronick C., Gaige T., Haakenson W., Haglund K., Holmes A., Harkins R., Kim K., Kruchowski S.S., Strong C.M., Grewal N., Goyea E., Hou S., Levy A., Martinka S., Mead K., McLellan M.D., Meyer R., Randall-Maher J., Tomlinson C., Dauphin-Kohlberg S., Kozlowicz-Reilly A., Shah N., Swearengen-Shahid S., Snider J., Strong J.T., Thompson J., Yoakum M., Leonard S., Pearman C., Trani L., Radionenko M., Waligorski J.E., Wang C., Rock S.M., Tin-Wollam A.-M., Maupin R., Latreille P., Wendl M.C., Yang S.-P., Pohl C., Wallis J.W., Spieth J., Bieri T.A., Berkowicz N., Nelson J.O., Osborne J., Ding L., Meyer R., Sabo A., Shotland Y., Sinha P., Wohldmann P.E., Cook L.L., Hickenbotham M.T., Eldred J., Williams D., Jones T.A., She X., Ciccarelli F.D., Izaurralde E., Taylor J., Schmutz J., Myers R.M., Cox D.R., Huang X., McPherson J.D., Mardis E.R., Clifton S.W., Warren W.C., Chinwalla A.T., Eddy S.R., Marra M.A., Ovcharenko I., Furey T.S., Miller W., Eichler E.E., Bork P., Suyama M., Torrents D., Waterston R.H., Wilson R.K.;
"Generation and annotation of the DNA sequences of human chromosomes 2 and 4.";
Nature 434:724-731(2005).
[4]
NUCLEOTIDE SEQUENCE [MRNA] OF 211-505 (ISOFORMS 1/2).
PubMed=2320002 [NCBI, ExPASy, EBI, Israel, Japan]
Kara C.J., Liou H.-C., Ivashkiv L.B., Glimcher L.H.;
"A cDNA for a human cyclic AMP response element-binding protein which is distinct from CREB and expressed preferentially in brain.";
Mol. Cell. Biol. 10:1347-1357(1990).
[5]
INTERACTION WITH UTF1.
DOI=10.1074/jbc.273.40.25840; PubMed=9748258 [NCBI, ExPASy, EBI, Israel, Japan]
Fukushima A., Okuda A., Nishimoto M., Seki N., Hori T.A., Muramatsu M.;
"Characterization of functional domains of an embryonic stem cell coactivator UTF1 which are conserved and essential for potentiation of ATF-2 activity.";
J. Biol. Chem. 273:25840-25849(1998).
[6]
PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-112, AND MASS SPECTROMETRY.
TISSUE=Epithelium;
DOI=10.1073/pnas.0404720101; PubMed=15302935 [NCBI, ExPASy, EBI, Israel, Japan]
Beausoleil S.A., Jedrychowski M., Schwartz D., Elias J.E., Villen J., Li J., Cohn M.A., Cantley L.C., Gygi S.P.;
"Large-scale characterization of HeLa cell nuclear phosphoproteins.";
Proc. Natl. Acad. Sci. U.S.A. 101:12130-12135(2004).
[7]
PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT THR-69; THR-71 AND SER-112, AND MASS SPECTROMETRY.
TISSUE=Epithelium;
DOI=10.1016/j.cell.2006.09.026; PubMed=17081983 [NCBI, ExPASy, EBI, Israel, Japan]
Olsen J.V., Blagoev B., Gnad F., Macek B., Kumar C., Mortensen P., Mann M.;
"Global, in vivo, and site-specific phosphorylation dynamics in signaling networks.";
Cell 127:635-648(2006).
[8]
PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-328, AND MASS SPECTROMETRY.
DOI=10.1021/pr0705441; PubMed=18220336 [NCBI, ExPASy, EBI, Israel, Japan]
Cantin G.T., Yi W., Lu B., Park S.K., Xu T., Lee J.-D., Yates J.R. III;
"Combining protein-based IMAC, peptide-based IMAC, and MudPIT for efficient phosphoproteomic analysis.";
J. Proteome Res. 7:1346-1351(2008).
[9]
PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT THR-69; THR-71 AND SER-112, AND MASS SPECTROMETRY.
DOI=10.1073/pnas.0805139105; PubMed=18669648 [NCBI, ExPASy, EBI, Israel, Japan]
Dephoure N., Zhou C., Villen J., Beausoleil S.A., Bakalarski C.E., Elledge S.J., Gygi S.P.;
"A quantitative atlas of mitotic phosphorylation.";
Proc. Natl. Acad. Sci. U.S.A. 105:10762-10767(2008).
[10]
STRUCTURE BY NMR OF 19-56.
DOI=10.1006/jmbi.1999.2620; PubMed=10092462 [NCBI, ExPASy, EBI, Israel, Japan]
Nagadoi A., Nakazawa K., Uda H., Okuno K., Maekawa T., Ishii S., Nishimura Y.;
"Solution structure of the transactivation domain of ATF-2 comprising a zinc finger-like subdomain and a flexible subdomain.";
J. Mol. Biol. 287:593-607(1999).
[11]
VARIANT [LARGE SCALE ANALYSIS] HIS-352.
DOI=10.1126/science.1133427; PubMed=16959974 [NCBI, ExPASy, EBI, Israel, Japan]
Sjoeblom T., Jones S., Wood L.D., Parsons D.W., Lin J., Barber T.D., Mandelker D., Leary R.J., Ptak J., Silliman N., Szabo S., Buckhaults P., Farrell C., Meeh P., Markowitz S.D., Willis J., Dawson D., Willson J.K.V., Gazdar A.F., Hartigan J., Wu L., Liu C., Parmigiani G., Park B.H., Bachman K.E., Papadopoulos N., Vogelstein B., Kinzler K.W., Velculescu V.E.;
"The consensus coding sequences of human breast and colorectal cancers.";
Science 314:268-274(2006).
Comments
Copyright
Copyrighted by the UniProt Consortium, see http://www.uniprot.org/terms. Distributed under the Creative Commons Attribution-NoDerivs License.
Cross-references
Sequence databases
EMBL
X15875; CAA33886.1; -; mRNA.[EMBL / GenBank / DDBJ] [CoDingSequence]
U16028; AAB64017.1; -; mRNA.[EMBL / GenBank / DDBJ] [CoDingSequence]
AC131958; AAX88876.1; -; Genomic_DNA.[EMBL / GenBank / DDBJ] [CoDingSequence]
AC074291; AAY15004.1; -; Genomic_DNA.[EMBL / GenBank / DDBJ] [CoDingSequence]
AC007435; -; NOT_ANNOTATED_CDS; Genomic_DNA.[EMBL / GenBank / DDBJ]
AC096649; -; NOT_ANNOTATED_CDS; Genomic_DNA.[EMBL / GenBank / DDBJ]
M31630; AAA35951.1; -; mRNA.[EMBL / GenBank / DDBJ] [CoDingSequence]
IPI IPI00234446; -.
IPI00748604; -.
PIR S05380; S05380.
RefSeq NP_001871.2; -.
UniGene Hs.592510
3D structure databases
PDB
1BHI; NMR; -; A=19-56.[ExPASy / RCSB / EBI]
1T2K; X-ray; 3.00 A; D=354-414.[ExPASy / RCSB / EBI]
Detailed list of linked structures.
PDBsum 1BHI; -.
1T2K; -.
ModBase P15336.
Protein-protein interaction databases
DIP DIP:632N; -.
IntAct P15336; 12.
PTM databases
PhosphoSite P15336; -.
Enzyme and pathway databases
Pathway_Interaction_DB il12_2pathway; IL12-mediated signaling events.
smad2_3nuclearpathway; Regulation of nuclear SMAD2/3 signaling.
p38alphabetadownstreampathway; Signaling mediated by p38-alpha and p38-beta.
Reactome REACT_6900; Signaling in Immune system.
Organism-specific databases
GeneCards GC02M175645; -.
HGNC HGNC:784; ATF2.
GenAtlas ATF2.
MIM 123811; gene. [NCBI / EBI]
PharmGKB PA25084; -.
Gene expression databases
ArrayExpress P15336; -.
Bgee P15336; -.
CleanEx HS_ATF2; -.
GermOnline ENSG00000115966; Homo sapiens.
Ontologies
GO
GO:0005829; Cellular component: cytosol (inferred from experiment from Reactome).
GO:0005634; Cellular component: nucleus (inferred from direct assay from MGI).
GO:0046983; Molecular function: protein dimerization activity (inferred from electronic annotation from InterPro).
GO:0003702; Molecular function: RNA polymerase II transcription factor activity (traceable author statement from ProtInc).
GO:0043565; Molecular function: sequence-specific DNA binding (inferred from electronic annotation from InterPro).
GO:0003713; Molecular function: transcription coactivator activity (traceable author statement from ProtInc).
GO:0003700; Molecular function: transcription factor activity (inferred from direct assay from MGI).
GO:0008270; Molecular function: zinc ion binding (inferred from electronic annotation from UniProtKB-KW).
GO:0006355; Biological process: regulation of transcription, DNA-dependent (inferred from electronic annotation from UniProtKB-KW).
GO:0006350; Biological process: transcription (inferred from electronic annotation from UniProtKB-KW).
QuickGo view.
Family and domain databases
InterPro IPR011616; bZIP_1.
IPR004827; TF_bZIP.
IPR016378; TF_cAMP-dep.
IPR007087; Znf_C2H2.
IPR015880; Znf_C2H2-like.
Graphical view of domain structure.
Pfam PF00170; bZIP_1; 1.
PF00096; zf-C2H2; 1.
Pfam graphical view of domain structure.
PIRSF PIRSF003153; ATF2_CRE-BP1; 1.
SMART SM00338; BRLZ; 1.
SM00355; ZnF_C2H2; 1.
SMART graphical view of domain structure.
PROSITE PS50217; BZIP; 1.
PS00036; BZIP_BASIC; 1.
PS00028; ZINC_FINGER_C2H2_1; 1.
PS50157; ZINC_FINGER_C2H2_2; 1.
PROSITE graphical view of domain structure (profiles).
Proteomic databases
PRIDE P15336; -.
Genome annotation databases
Ensembl ENSG00000115966; Homo sapiens. [Contig view]
GeneID 1386; -.
KEGG hsa:1386; -.
Phylogenomic databases
HOGENOM P15336; -.
HOVERGEN P15336; -.
OMA P15336; QNEVTLL.
Other
NextBio 5631; -.
SOURCE ATF2; Homo sapiens.
ProtoNet P15336.
UniRef View cluster of proteins with at least 50% / 90% / 100% identity.
Keywords
3D-structure; Activator; Alternative splicing; DNA-binding; Metal-binding; Nucleus; Phosphoprotein; Polymorphism; Transcription; Transcription regulation; Zinc; Zinc-finger.
Features
SEVIEWER logo Feature table viewer FT aligner logo Feature aligner
KeyFrom   To Length Description FTId
CHAIN   1   505  505     Cyclic AMP-dependent transcription factor ATF-2. PRO_0000076577
DOMAIN   380   408  29     Leucine-zipper. 
ZN_FING   25    49  25     C2H2-type. 
DNA_BIND   351   374  24     Basic motif. 
MOD_RES   69    69        Phosphothreonine; by MAPK14. 
MOD_RES   71    71        Phosphothreonine; by MAPK14. 
MOD_RES   112   112        Phosphoserine. 
MOD_RES   328   328        Phosphoserine. 
VAR_SEQ   1   176        Missing (in isoform 2). VSP_000587
VAR_SEQ   177   185        TSSDSSVII -> MSTAYFQMM (in isoform 2). VSP_000588
VARIANT   352   352  1     D -> H (in a breast cancer sample; somatic mutation). VAR_035999 
CONFLICT   209   209        V -> L (in Ref. 2; AAB64017). 
CONFLICT   223   223        N -> S (in Ref. 1; CAA33886). 
CONFLICT   311   311        R -> L (in Ref. 2; AAB64017). 
TURN   30    32  3      
STRAND   35    38  4      
HELIX   39    50  12      
TURN   51    55  5      
HELIX   355   412  58      
Sequence information
Length: 505 AA [This is the length of the unprocessed precursor] Molecular weight: 54537 Da [This is the MW of the unprocessed precursor] CRC64: 0190EEFAEC8891A7 [This is a checksum on the sequence]
        10         20         30         40         50         60 
MKFKLHVNSA RQYKDLWNMS DDKPFLCTAP GCGQRFTNED HLAVHKHKHE MTLKFGPARN 

        70         80         90        100        110        120 
DSVIVADQTP TPTRFLKNCE EVGLFNELAS PFENEFKKAS EDDIKKMPLD LSPLATPIIR 

       130        140        150        160        170        180 
SKIEEPSVVE TTHQDSPLPH PESTTSDEKE VPLAQTAQPT SAIVRPASLQ VPNVLLTSSD 

       190        200        210        220        230        240 
SSVIIQQAVP SPTSSTVITQ APSSNRPIVP VPGPFPLLLH LPNGQTMPVA IPASITSSNV 

       250        260        270        280        290        300 
HVPAAVPLVR PVTMVPSVPG IPGPSSPQPV QSEAKMRLKA ALTQQHPPVT NGDTVKGHGS 

       310        320        330        340        350        360 
GLVRTQSEES RPQSLQQPAT STTETPASPA HTTPQTQSTS GRRRRAANED PDEKRRKFLE 

       370        380        390        400        410        420 
RNRAAASRCR QKRKVWVQSL EKKAEDLSSL NGQLQSEVTL LRNEVAQLKQ LLLAHKDCPV 

       430        440        450        460        470        480 
TAMQKKSGYH TADKDDSSED ISVPSSPHTE AIQHSSVSTS NGVSSTSKAE AVATSVLTQM 

       490        500 
ADQSTEPALS QIVMAPSSQS QPSGS 

P15336 in FASTA format

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