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UniProtKB/Swiss-Prot entry P15274


[Entry info] [Name and origin] [References] [Comments] [Cross-references] [Keywords] [Features] [Sequence] [Tools]

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Entry information
Entry name AMPD_YEAST
Primary accession number P15274
Secondary accession numbers None
Integrated into Swiss-Prot on April 1, 1990
Sequence was last modified on February 1, 1996 (Sequence version 2)
Annotations were last modified on    July 22, 2008 (Entry version 78)
Name and origin of the protein
Protein name AMP deaminase
Synonyms EC 3.5.4.6
Myoadenylate deaminase
Gene name
Name: AMD1
Synonyms: AMD
OrderedLocusNames: YML035C
From
Saccharomyces cerevisiae (Baker's yeast) [TaxID: 4932] 
Taxonomy Eukaryota; Fungi; Dikarya; Ascomycota; Saccharomycotina; Saccharomycetes; Saccharomycetales; Saccharomycetaceae; Saccharomyces.
Protein existence 1: Evidence at protein level;
References
[1]
NUCLEOTIDE SEQUENCE [GENOMIC DNA].
DOI=10.1021/bi00448a009; PubMed=2690949 [NCBI, ExPASy, EBI, Israel, Japan]
Meyer S.L., Kvalnes-Krick K.L., Schramm V.L.;
"Characterization of AMD, the AMP deaminase gene in yeast. Production of amd strain, cloning, nucleotide sequence, and properties of the protein.";
Biochemistry 28:8734-8743(1989).
[2]
NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
STRAIN=ATCC 204511 / S288c / AB972;
PubMed=9169872 [NCBI, ExPASy, EBI, Israel, Japan]
Bowman S., Churcher C.M., Badcock K., Brown D., Chillingworth T., Connor R., Dedman K., Devlin K., Gentles S., Hamlin N., Hunt S., Jagels K., Lye G., Moule S., Odell C., Pearson D., Rajandream M.A., Rice P., Skelton J., Walsh S.V., Whitehead S., Barrell B.G.;
"The nucleotide sequence of Saccharomyces cerevisiae chromosome XIII.";
Nature 387:90-93(1997).
[3]
LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
DOI=10.1038/nature02046; PubMed=14562106 [NCBI, ExPASy, EBI, Israel, Japan]
Ghaemmaghami S., Huh W.-K., Bower K., Howson R.W., Belle A., Dephoure N., O'Shea E.K., Weissman J.S.;
"Global analysis of protein expression in yeast.";
Nature 425:737-741(2003).
[4]
PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-138, AND MASS SPECTROMETRY.
DOI=10.1074/mcp.M400219-MCP200; PubMed=15665377 [NCBI, ExPASy, EBI, Israel, Japan]
Gruhler A., Olsen J.V., Mohammed S., Mortensen P., Faergeman N.J., Mann M., Jensen O.N.;
"Quantitative phosphoproteomics applied to the yeast pheromone signaling pathway.";
Mol. Cell. Proteomics 4:310-327(2005).
[5]
PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-58; SER-61 AND SER-138, AND MASS SPECTROMETRY.
DOI=10.1021/pr060559j; PubMed=17330950 [NCBI, ExPASy, EBI, Israel, Japan]
Li X., Gerber S.A., Rudner A.D., Beausoleil S.A., Haas W., Villen J., Elias J.E., Gygi S.P.;
"Large-scale phosphorylation analysis of alpha-factor-arrested Saccharomyces cerevisiae.";
J. Proteome Res. 6:1190-1197(2007).
[6]
PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-138, AND MASS SPECTROMETRY.
DOI=10.1073/pnas.0607084104; PubMed=17287358 [NCBI, ExPASy, EBI, Israel, Japan]
Chi A., Huttenhower C., Geer L.Y., Coon J.J., Syka J.E.P., Bai D.L., Shabanowitz J., Burke D.J., Troyanskaya O.G., Hunt D.F.;
"Analysis of phosphorylation sites on proteins from Saccharomyces cerevisiae by electron transfer dissociation (ETD) mass spectrometry.";
Proc. Natl. Acad. Sci. U.S.A. 104:2193-2198(2007).
[7]
PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-58; SER-61; THR-136 AND SER-138, AND MASS SPECTROMETRY.
DOI=10.1073/pnas.0701622104; PubMed=17563356 [NCBI, ExPASy, EBI, Israel, Japan]
Smolka M.B., Albuquerque C.P., Chen S.H., Zhou H.;
"Proteome-wide identification of in vivo targets of DNA damage checkpoint kinases.";
Proc. Natl. Acad. Sci. U.S.A. 104:10364-10369(2007).
[8]
PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-19; SER-26; SER-58; SER-61; SER-138 AND SER-140, AND MASS SPECTROMETRY.
DOI=10.1074/mcp.M700468-MCP200; PubMed=18407956 [NCBI, ExPASy, EBI, Israel, Japan]
Albuquerque C.P., Smolka M.B., Payne S.H., Bafna V., Eng J., Zhou H.;
"A multidimensional chromatography technology for in-depth phosphoproteome analysis.";
Mol. Cell. Proteomics 0:0-0(2008).
Comments
Copyright
Copyrighted by the UniProt Consortium, see http://www.uniprot.org/terms. Distributed under the Creative Commons Attribution-NoDerivs License.
Cross-references
Sequence databases
EMBL
M30449; AAA34420.1; -; Genomic_DNA.[EMBL / GenBank / DDBJ] [CoDingSequence]
Z46659; CAA86620.1; -; Genomic_DNA.[EMBL / GenBank / DDBJ] [CoDingSequence]
PIR S49744; S49744.
RefSeq NP_013677.1; -.
3D structure databases
ModBase P15274.
Protein-protein interaction databases
DIP DIP:1949N; -.
IntAct P15274; -.
Organism-specific databases
CYGD YML035c; -.
SGD S000004498; AMD1.
Yeast-GFP YML035C.
Gene expression databases
ArrayExpress P15274; -.
GermOnline YML035C; Saccharomyces cerevisiae.
Ontologies
GO
GO:0005737; Cellular component: cytoplasm (inferred from direct assay from SGD).
GO:0003876; Molecular function: AMP deaminase activity (inferred from direct assay from SGD).
GO:0005515; Molecular function: protein binding (inferred from physical interaction from IntAct).
GO:0006163; Biological process: purine nucleotide metabolic process (inferred by curator from SGD).
QuickGo view.
Family and domain databases
InterPro IPR006650; A/AMP_deam_AS.
IPR001365; A/AMP_deaminase.
IPR006329; AMP_deaminase.
IPR016297; AMP_deaminase_met.
Graphical view of domain structure.
Pfam PF00962; A_deaminase; 1.
Pfam graphical view of domain structure.
PIRSF PIRSF001251; AMP_deaminase_met; 1.
TIGRFAMs TIGR01429; AMP_deaminase; 1.
PROSITE PS00485; A_DEAMINASE; 1.
BLOCKS P15274.
Proteomic databases
PeptideAtlas P15274; -.
Genome annotation databases
Ensembl YML035C; Saccharomyces cerevisiae. [Contig view]
GeneID 854973; -.
GenomeReviews Z71257_GR; YML035C.
KEGG sce:YML035C; -.
NMPDR fig|4932.3.peg.4717; -.
Phylogenomic databases
HOGENOM P15274; -.
Other
LinkHub P15274; -.
ProtoNet P15274.
UniRef View cluster of proteins with at least 50% / 90% / 100% identity.
Keywords
Complete proteome; Hydrolase; Nucleotide metabolism; Phosphoprotein.
Features
SEVIEWER logo Feature table viewer
KeyFrom   To Length Description FTId
CHAIN   1   810  810     AMP deaminase. PRO_0000194414
ACT_SITE   422   422        Potential. 
ACT_SITE   631   631        Potential. 
ACT_SITE   707   707        Potential. 
ACT_SITE   708   708        Potential. 
MOD_RES   19    19        Phosphoserine. 
MOD_RES   26    26        Phosphoserine. 
MOD_RES   58    58        Phosphoserine. 
MOD_RES   61    61        Phosphoserine. 
MOD_RES   136   136        Phosphothreonine. 
MOD_RES   138   138        Phosphoserine. 
MOD_RES   140   140        Phosphoserine. 
CONFLICT   568   568        F -> C (in Ref. 1; AAA34420). 
Sequence information
Length: 810 AA [This is the length of the unprocessed precursor] Molecular weight: 93302 Da [This is the MW of the unprocessed precursor] CRC64: 7A6DCB43B9B45C93 [This is a checksum on the sequence]
        10         20         30         40         50         60 
MDNQATQRLN DLSLEPAPSH DEQDGSGLVI DIDQRKIGDE QAGVVVDDET PPLEQQDSHE 

        70         80         90        100        110        120 
SLAADSRNAN FSYHENQQLL ENGTKQLALD EHDSHSAILE QPSHSTNCSS SNIAAMNKGH 

       130        140        150        160        170        180 
DSADHASQNS GGKPRTLSAS AQHILPETLK SFAGAPVVNK QVRTSASYKM GMLADDASQQ 

       190        200        210        220        230        240 
FLDDPSSELI DLYSKVAECR NLRAKYQTIS VQNDDQNPKN KPGWVVYPPP PKPSYNSDTK 

       250        260        270        280        290        300 
TVVPVTNKPD AEVFDFTKCE IPGEDPDWEF TLNDDDSYVV HRSGKTDELI AQIPTLRDYY 

       310        320        330        340        350        360 
LDLEKMISIS SDGPAKSFAY RRLQYLEARW NLYYLLNEYQ ETSVSKRNPH RDFYNVRKVD 

       370        380        390        400        410        420 
THVHHSACMN QKHLLRFIKH KLRHSKDEKV IFRDGKLLTL DEVFRSLHLT GYDLSIDTLD 

       430        440        450        460        470        480 
MHAHKDTFHR FDKFNLKYNP IGESRLREIF LKTNNYIKGT YLADITKQVI FDLENSKYQN 

       490        500        510        520        530        540 
CEYRISVYGR SLDEWDKLAS WVIDNKVISH NVRWLVQIPR LYDIYKKTGI VQSFQDICKN 

       550        560        570        580        590        600 
LFQPLFEVTK NPQSHPKLHV FLQRVIGFDS VDDESKVDRR FHRKYPKPSL WEAPQNPPYS 

       610        620        630        640        650        660 
YYLYYLYSNV ASLNQWRAKR GFNTLVLRPH CGEAGDPEHL VSAYLLAHGI SHGILLRKVP 

       670        680        690        700        710        720 
FVQYLYYLDQ VGIAMSPLSN NALFLTYDKN PFPRYFKRGL NVSLSTDDPL QFSYTREPLI 

       730        740        750        760        770        780 
EEYSVAAQIY KLSNVDMCEL ARNSVLQSGW EAQIKKHWIG KDFDKSGVEG NDVVRTNVPD 

       790        800        810 
IRINYRYDTL STELELVNHF ANFKRTIEEK 

P15274 in FASTA format

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