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- FUNCTION: Initiates DNA unwinding and replication via elaborate interactions with the viral origin of replication (By similarity).
- FUNCTION: Large T antigen has both transforming and trans-activating activities. Disrupts the function of host retinoblastoma protein RB1/pRb, which is a key regulator of the cell cycle. Induces the disassembly of the E2F1 transcription factors from RB1, with subsequent transcriptional activation of E2F1-regulated S-phase genes. Inactivation of the ability of RB1 to arrest the cell cycle is critical for cellular transformation, uncontrolled cellular growth and proliferation induced by viral infection. Stimulation of progression from G1 to S phase allows the virus to efficiently use the cellular DNA replicating machinery to achieve viral genome replication. Interferes with histone deacetylation mediated by HDAC1, leading to activation of transcription (By similarity).
- SUBUNIT: Forms homohexamers in the presence of ATP. Interaction with host RB1 induces the aberrant dissociation of RB1-E2F1 complex thereby disrupting RB1's activity (By similarity).
- SUBCELLULAR LOCATION: Nucleus.
- ALTERNATIVE PRODUCTS:
2 named isoforms [FASTA] produced by alternative splicing.
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| Name | Small t antigen |
| Isoform ID | P15000-1 |
| This isoform is stored in UniProtKB/Swiss-Prot entry P15000. |
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- DOMAIN: The zinc finger region contributes to protein-protein interactions essential for the assembly of stable T-antigen hexamers at the origin of replication and hexamers are needed for subsequent alterations in the structure of origin DNA (By similarity).
- PTM: Phosphorylated on both serine and threonine residues (By similarity).
- SIMILARITY: Contains 1 J domain.
- SIMILARITY: Contains 1 SF3 helicase domain.
- SIMILARITY: Contains 1 T-ag D1-type zinc finger.
- SIMILARITY: Contains 1 T-ag OBD DNA-binding domain.
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Copyrighted by the UniProt Consortium, see http://www.uniprot.org/terms.
Distributed under the Creative Commons Attribution-NoDerivs License.
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| Length: 691 AA [This is the length of the unprocessed precursor] |
Molecular weight: 80129 Da [This is the MW of the unprocessed precursor] |
CRC64: 2F26992D3C441A84 [This is a checksum on the sequence] |
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10 20 30 40 50 60
MDKVLNREES MELMDLLGLE RAAWGNLPLM RKAYLKKCKE FHPDKGGDED KMKRMNTLYK
70 80 90 100 110 120
KMEQDVKVAH QPDFGTWNSS EVPTYGTEEW ESWWSSFNEK WDEDLFCHED MFASDEEATA
130 140 150 160 170 180
DSQHSTPPKK KRKVEDPKDF PSDLHQFLSQ AVFSNRTLAC FAVYTTKEKA QILYKKLMEK
190 200 210 220 230 240
YSVTFISRHM CAGHNIIFFL TPHRHRVSAI NNFCQKLCTF SFLICKGVNK EYLLYSALTR
250 260 270 280 290 300
DPYHIIEESI QGGLKEHDFN PEEPEETKQV SWKLITEYAV ETKCEDVFLL LGMYLEFQYN
310 320 330 340 350 360
VEECKKCQKK DQPYHFKYHE KHFANAIIFA ESKNQKSICQ QAVDTVLAKK RVDTLHMTRE
370 380 390 400 410 420
EMLTERFNHI LDKMDLIFGA HGNAVLEQYM AGVAWLHCLL PKMDSVIFDF LHCVVFNVPK
430 440 450 460 470 480
RRYWLFKGPI DSGKTTLAAG LLDLCGGKAL NVNLPMERLT FELGVAIDQY MVVFEDVKGT
490 500 510 520 530 540
GAESKDLPSG HGINNLDSLR DYLDGSVKVN LEKKHLNKRT QIFPPGLVTM NEYPVPKTLQ
550 560 570 580 590 600
ARFVRQIDFR PKIYLRKSLQ NSEFLLEKRI LQSGMTLLLL LIWFRPVADF SKDIQSRIVE
610 620 630 640 650 660
WKERLDSEIS MYTFSRMKYN ICMGKCILDI TREEDSETED SGHGSSTESQ SQCSSQVSDT
670 680 690
SAPDSENPHS QELHLCKGFQ CFKRPKTPPP K
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P14999 in FASTA format |
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