ID   PDE1A_BOVIN             Reviewed;         530 AA.
AC   P14100; Q08E30; Q28063;
DT   01-JAN-1990, integrated into UniProtKB/Swiss-Prot.
DT   23-JAN-2007, sequence version 3.
DT   02-SEP-2008, entry version 70.
DE   RecName: Full=Calcium/calmodulin-dependent 3',5'-cyclic nucleotide phosphodiesterase 1A;
DE            Short=Cam-PDE 1A;
DE            EC=3.1.4.17;
DE   AltName: Full=61 kDa Cam-PDE;
GN   Name=PDE1A;
OS   Bos taurus (Bovine).
OC   Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
OC   Mammalia; Eutheria; Laurasiatheria; Cetartiodactyla; Ruminantia;
OC   Pecora; Bovidae; Bovinae; Bos.
OX   NCBI_TaxID=9913;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [MRNA].
RC   TISSUE=Brain;
RX   MEDLINE=93107074; PubMed=7678006;
RA   Sonnenburg W.K., Seger D., Beavo J.A.;
RT   "Molecular cloning of a cDNA encoding the '61-kDa' calmodulin-
RT   stimulated cyclic nucleotide phosphodiesterase. Tissue-specific
RT   expression of structurally related isoforms.";
RL   J. Biol. Chem. 268:645-652(1993).
RN   [2]
RP   NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1).
RC   TISSUE=Lung;
RX   MEDLINE=96125075; PubMed=8537356; DOI=10.1074/jbc.270.52.30989;
RA   Sonnenburg W.K., Seger D., Kwak K.S., Huang J., Charbonneau H.,
RA   Beavo J.A.;
RT   "Identification of inhibitory and calmodulin-binding domains of the
RT   PDE1A1 and PDE1A2 calmodulin-stimulated cyclic nucleotide
RT   phosphodiesterases.";
RL   J. Biol. Chem. 270:30989-31000(1995).
RN   [3]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1).
RC   STRAIN=Hereford; TISSUE=Hippocampus;
RG   NIH - Mammalian Gene Collection (MGC) project;
RL   Submitted (SEP-2006) to the EMBL/GenBank/DDBJ databases.
RN   [4]
RP   PROTEIN SEQUENCE OF 2-530 (ISOFORM 2).
RC   TISSUE=Brain;
RX   MEDLINE=91329365; PubMed=1651111; DOI=10.1021/bi00246a009;
RA   Charbonneau H., Kumar S., Novack J.P., Blumenthal D.K., Griffin P.R.,
RA   Shabanowitz J., Hunt D.F., Beavo J.A., Walsh K.A.;
RT   "Evidence for domain organization within the 61-kDa calmodulin-
RT   dependent cyclic nucleotide phosphodiesterase from bovine brain.";
RL   Biochemistry 30:7931-7940(1991).
RN   [5]
RP   PROTEIN SEQUENCE OF 194-427.
RC   TISSUE=Brain;
RX   MEDLINE=87092242; PubMed=3025833;
RA   Charbonneau H., Beier N., Walsh K.A., Beavo J.A.;
RT   "Identification of a conserved domain among cyclic nucleotide
RT   phosphodiesterases from diverse species.";
RL   Proc. Natl. Acad. Sci. U.S.A. 83:9308-9312(1986).
CC   -!- CATALYTIC ACTIVITY: Nucleoside 3',5'-cyclic phosphate + H(2)O =
CC       nucleoside 5'-phosphate.
CC   -!- ENZYME REGULATION: Type I PDE are activated by the binding of
CC       calmodulin in the presence of Ca(2+).
CC   -!- SUBUNIT: Homodimer.
CC   -!- INTERACTION:
CC       P62168:Freq (xeno); NbExp=1; IntAct=EBI-907809, EBI-907774;
CC       P84076:Hpca (xeno); NbExp=1; IntAct=EBI-907809, EBI-908193;
CC       P61602:NCALD; NbExp=1; IntAct=EBI-907809, EBI-908133;
CC   -!- ALTERNATIVE PRODUCTS:
CC       Event=Alternative splicing; Named isoforms=2;
CC       Name=2; Synonyms=PDE1A2;
CC         IsoId=P14100-1; Sequence=Displayed;
CC       Name=1; Synonyms=PDE1A1;
CC         IsoId=P14100-2; Sequence=VSP_004546;
CC   -!- SIMILARITY: Belongs to the cyclic nucleotide phosphodiesterase
CC       family.
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DR   EMBL; M90358; AAA74560.1; -; mRNA.
DR   EMBL; L34069; AAA92555.1; -; mRNA.
DR   EMBL; BC123449; AAI23450.1; -; mRNA.
DR   PIR; A45334; A45334.
DR   RefSeq; NP_776839.1; -.
DR   UniGene; Bt.512; -.
DR   HSSP; Q08499; 1OYN.
DR   SMR; P14100; 142-471.
DR   IntAct; P14100; -.
DR   Ensembl; ENSBTAG00000012100; Bos taurus.
DR   GeneID; 281969; -.
DR   KEGG; bta:281969; -.
DR   HOVERGEN; P14100; -.
DR   GO; GO:0005515; F:protein binding; IPI:IntAct.
DR   InterPro; IPR003607; Met-dep_phosphohydro_HD.
DR   InterPro; IPR002073; PDEase.
DR   InterPro; IPR013706; PDEase_N.
DR   Pfam; PF00233; PDEase_I; 1.
DR   Pfam; PF08499; PDEase_I_N; 1.
DR   PRINTS; PR00387; PDIESTERASE1.
DR   SMART; SM00471; HDc; 1.
DR   PROSITE; PS00126; PDEASE_I; 1.
PE   1: Evidence at protein level;
KW   Alternative splicing; Calmodulin-binding; cAMP; cGMP;
KW   Direct protein sequencing; Hydrolase.
FT   INIT_MET      1      1       Removed.
FT   CHAIN         2    530       Calcium/calmodulin-dependent 3',5'-cyclic
FT                                nucleotide phosphodiesterase 1A.
FT                                /FTId=PRO_0000198784.
FT   REGION       24     44       Calmodulin-binding.
FT   REGION      193    501       Catalytic (By similarity).
FT   VAR_SEQ       1     34       MGSTATETEELENTTFKYLIGEQTEKMWQRLKGI -> MDD
FT                                HVTIRRKHLQRPIFR (in isoform 1).
FT                                /FTId=VSP_004546.
FT   CONFLICT    237    237       H -> G (in Ref. 5; AA sequence).
FT   CONFLICT    321    321       N -> W (in Ref. 5; AA sequence).
SQ   SEQUENCE   530 AA;  60843 MW;  24CF83E5211AE06F CRC64;
     MGSTATETEE LENTTFKYLI GEQTEKMWQR LKGILRCLVK QLEKGDVNVI DLKKNIEYAA
     SVLEAVYIDE TRRLLDTDDE LSDIQSDSVP SEVRDWLAST FTRKMGMMKK KSEEKPRFRS
     IVHVVQAGIF VERMYRKSYH MVGLAYPEAV IVTLKDVDKW SFDVFALNEA SGEHSLKFMI
     YELFTRYDLI NRFKIPVSCL IAFAEALEVG YSKYKNPYHN LIHAADVTQT VHYIMLHTGI
     MHWLTELEIL AMVFAAAIHD YEHTGTTNNF HIQTRSDVAI LYNDRSVLEN HHVSAAYRLM
     QEEEMNVLIN LSKDDWRDLR NLVIEMVLST DMSGHFQQIK NIRNSLQQPE GLDKAKTMSL
     ILHAADISHP AKSWKLHHRW TMALMEEFFL QGDKEAELGL PFSPLCDRKS TMVAQSQIGF
     IDFIVEPTFS LLTDSTEKII IPLIEEDSKT KTPSYGASRR SNMKGTTNDG TYSPDYSLAS
     VDLKSFKNSL VDIIQQNKER WKELAAQGEP DPHKNSDLVN AEEKHAETHS
//