RNA-directed RNA polymerase
     (EC 2.7.7.48)
mRNA (guanine-N(7)-)-methyltransferase
     (EC 2.1.1.56)
mRNA guanylyltransferase
     (EC 2.7.7.-)

Gene name

Name:

From

Vesicular stomatitis New Jersey virus (strain Hazelhurst subtype Hazelhurst) (VSNJV)

[TaxID: 11281]

Taxonomy

Viruses; ssRNA negative-strand viruses; Mononegavirales; Rhabdoviridae; Dimarhabdovirus supergroup; Vesiculovirus.

Virus hosts

Aedes [TaxID: 7158]
Bos taurus (Bovine) [TaxID: 9913]
Culicoides [TaxID: 58271]
Equus asinus (Donkey) [TaxID: 9793]
Equus caballus (Horse) [TaxID: 9796]
Homo sapiens (Human) [TaxID: 9606]
Lutzomyia [TaxID: 252607]
Musca domestica (House fly) [TaxID: 7370]
Simuliidae (black flies) [TaxID: 7190]
Sus scrofa (Pig) [TaxID: 9823]

Protein existence

3: Inferred from homology;

References

[1]

NUCLEOTIDE SEQUENCE [GENOMIC RNA].
DOI=10.1016/0042-6822(88)90276-0; PubMed=2833012 [NCBI, ExPASy, EBI, Israel, Japan]
Feldhaus A.L., Lesnaw J.A.;
"Nucleotide sequence of the L gene of vesicular stomatitis virus (New Jersey): identification of conserved domains in the New Jersey and Indiana L proteins.";
Virology 163:359-368(1988).

Comments

FUNCTION: Displays RNA-directed RNA polymerase, mRNA guanylyl transferase, mRNA (guanine-N(7)-)-methyltransferase and poly(A) synthetase activities. The viral mRNA guanylyl transferase displays a different biochemical reaction than the cellular enzyme. The template is composed of the viral RNA tightly encapsidated by the nucleoprotein (N). Functions either as transcriptase or as replicase. The transcriptase synthesizes subsequently five subgenomic RNAs, assuring their capping and polyadenylation by a stuttering mechanism. The replicase mode is dependent on intracellular N protein concentration. In this mode, the polymerase replicates the whole viral genome without recognizing the transcriptional signals (By similarity).
CATALYTIC ACTIVITY: Nucleoside triphosphate + RNA(n) = diphosphate + RNA(n+1).
CATALYTIC ACTIVITY: S-adenosyl-L-methionine + G(5')pppR-RNA = S-adenosyl-L-homocysteine + m₇G(5')pppR-RNA.
SUBUNIT: Interacts with the P protein (By similarity).
SUBCELLULAR LOCATION: Virion (Potential). Cytoplasm (By similarity).
SIMILARITY: Belongs to the rhabdoviridae protein L family.
SIMILARITY: Contains 1 RdRp catalytic domain.

Copyright

Copyrighted by the UniProt Consortium, see http://www.uniprot.org/terms. Distributed under the Creative Commons Attribution-NoDerivs License.

Cross-references

Sequence databases

EMBL

M20166; AAA48440.1; -; Genomic_RNA.

[EMBL / GenBank / DDBJ] [CoDingSequence]

PIR

A28602; ZLVNNJ.

3D structure databases

ModBase

P13615.

Family and domain databases

InterPro

IPR017234; RNA-dir_RNA_pol_rhabdovir.
IPR014023; RNA_pol_cat.
IPR001016; RNA_pol_L_viral.
Graphical view of domain structure.

Pfam

PF00946; Paramyx_RNA_pol; 1.
Pfam graphical view of domain structure.

PIRSF

PIRSF037546; RNA_pol_RhabdoV_sub; 1.

PROSITE

PS50526; RDRP_SSRNA_NEG_NONSEG; 1.
PROSITE graphical view of domain structure (profiles).

Other

View cluster of proteins with at least 50% / 90% / 100% identity.

Keywords

ATP-binding; Cytoplasm; Methyltransferase; mRNA capping; mRNA processing; Multifunctional enzyme; Nucleotide-binding; Nucleotidyltransferase; RNA replication; RNA-directed RNA polymerase; S-adenosyl-L-methionine; Transferase; Virion.

Features

Feature table viewer

Feature aligner

`Key`	`From To`	`Length`	`Description`	`FTId`
`CHAIN`	`1 2109`	`2109`	`Large structural protein.`	`PRO_0000222845`
`DOMAIN`	`598 784`	`187`	`RdRp catalytic.`
`NP_BIND`	`1667 1676`	`10`	`ATP (Potential).`

Sequence information

Length: 2109 AA [This is the length of the unprocessed precursor]

Molecular weight: 241583 Da [This is the MW of the unprocessed precursor]

CRC64: 52FF2182953E9F79 [This is a checksum on the sequence]

        10         20         30         40         50         60 
MDFNLIEDST HWEEEESDFL LRDILSKEDQ MSYLNSADYN LNSPLISDDM VYLIKRMNHE 

        70         80         90        100        110        120 
EVPPIWRSKE WDSPLDMLKG CQAQPLSHQD MHNWFGTWIQ NIQHDSAQGF TFLKEVDKEA 

       130        140        150        160        170        180 
EMTYDLVSTF LKGWVGKEYP FKPKGREIDS IALVGPLCQK FLDLHKVTLI LNAVSLGETK 

       190        200        210        220        230        240 
ELLTTFKGKY RMSCENIPIA RLRLPSLGPV FMCKGWTYIH KERVLMDRNF LLMCKDVIIG 

       250        260        270        280        290        300 
RMQTFLSMIG RSDNKFSPDQ IYTLANVYRI GDRILEQCGN RAYDLIKMIE PICNLKMMEL 

       310        320        330        340        350        360 
ARLHRPKIPK FPHFEEHLKG SVRELTKKSN KIQALYDLIM SIKDVDLVLV VYGSFRHWGH 

       370        380        390        400        410        420 
PFIDYFEGLK KLYTQVNMEK NIDKEHPQQL ASDLARLVLL KQFSESKKWF VDLSKMPPKH 

       430        440        450        460        470        480 
PFYEHVVNKT WPTAAKIQDF GDNWHKLPLT QCFEIPDLID PSVIYSDKSH SMNKKEVIQH 

       490        500        510        520        530        540 
VRTKPNIPIP SKKELQTMLT NKATNWKVFL KDIDGNGLDD DDLIIGLKGK ERELKIAGRF 

       550        560        570        580        590        600 
FSLMSWRLRE YFVITEYLIK TYYVPLFKGL TMADDLTSVI KKMMDSSSGQ GLDDYSSVCL 

       610        620        630        640        650        660 
ANHIDYEQWN NHQRKESNGP IFRVMGQFLG YPSLIERIHE FFEKSLIYYN GLPDLLTIRN 

       670        680        690        700        710        720 
GTLCNSTKHR VCWNGQKGGL EGLRQKGWSI VNLLVIQREA KIRNTAVKVL AQGDNQVICT 

       730        740        750        760        770        780 
QYKTRKTRSE LELRAVLHQM AGNNNKIMEE IKRGTEKLGL IINDDETMQS ADYLNYGKIP 

       790        800        810        820        830        840 
IFRGVIRGLE TKRWSRVTCV TNDQIPTCAN LMSSVSTNAL TVAHFAESPI NAMIQYNYFG 

       850        860        870        880        890        900 
TFARLLLFMH DPAIRQSLYT VKEKIPGLHT RTFKYAVLYL DPSIGGECGM ALSRFLIRAF 

       910        920        930        940        950        960 
PDPETESLSF WKFIYEHARS LHLKKMAVMF GDLPIAKFRI EHINKLLEDP TSLNISMGMS 

       970        980        990       1000       1010       1020 
PANLLKSEVK KCLIESRSSI KNEIIKDATI YMHQEEEKLR GFLWSINPLF PRFLSEFKAG 

      1030       1040       1050       1060       1070       1080 
TFLGVSEALI NLFQNSRTIR NSFKRRYHKD LDELIIKSEI SSLSHLGSMH YRLGDNHIWS 

      1090       1100       1110       1120       1130       1140 
CSASRADVLR YKSWTRKVVG TTVPHPLEMH GSPSKKENPC QLCNSSGLTY ISVHCPKGIT 

      1150       1160       1170       1180       1190       1200 
DVFNRRGPLP AYLGSNTSES TSILQPWEKE SKIPIIKRAT RLRDAISWFI PPESPLSTCI 

      1210       1220       1230       1240       1250       1260 
LNNIRALTGE DWSSKQHGFK RTGSALHRFS TSRMSNGGFA SQSPATLTRM IATTDTMRDF 

      1270       1280       1290       1300       1310       1320 
GTKNYDFMFQ ASLLYGQMTT SISRYGSPGS CTDHYHIRCK GCIREIEEVE LNTSLEYRTP 

      1330       1340       1350       1360       1370       1380 
VVYHILEKWR NNTGSWGHQI KQLKPAEGNW ESLSPVEQSY QVARCIGFLY GELTHKKSRQ 

      1390       1400       1410       1420       1430       1440 
ADDSSLFPLS IQLKVRGRGF LQGLLDGLMR SSCCQVIHRR SVSTLKRPAN AVYGGLIYLI 

      1450       1460       1470       1480       1490       1500 
DKLSASSPFL SLVRTGPIRQ ELEQVPHKMS TSYPTNIRDL GSIVRNYFKY QCRPVERGHY 

      1510       1520       1530       1540       1550       1560 
KTYYNQIWLF SDVLSTEFIG PMRISSSLLK LLYRSSLTKK DKEELRELAA LSSNLRSGED 

      1570       1580       1590       1600       1610       1620 
WDDLHIKFFS NDLLFCPAEI RHACKFGIQK ENEDIALYPN WGIEYIGNVI DIPVFYRAQN 

      1630       1640       1650       1660       1670       1680 
VQKDIRIPLR IQNPLMSGLR LGQLPTGAHY KMRTIISRLK IPYHDFLACG DGSGGMTVAL 

      1690       1700       1710       1720       1730       1740 
LRLNRASRGI FNSLLDLSDT MLRGSSPEPP SALETLGGER SRCVNGDSCW EHPSDLSDKN 

      1750       1760       1770       1780       1790       1800 
TWKYFLHVKK GCGMSINLIT MDMEVKDPTM SYKIELLVRQ YVPVLLESDC CLIYKTYGTY 

      1810       1820       1830       1840       1850       1860 
IATQKDNSLT LIGSLFHSVQ LVQTDLSSSN TSELYLVCRR LKDYIDTPFV DWIELYDSWE 

      1870       1880       1890       1900       1910       1920 
NQYAFKDIKD EFHRARSLKP ETTLVGIPPQ FIPDPGVNLE TLFQIAGVPT GVAHGVIITS 

      1930       1940       1950       1960       1970       1980 
MQSKNKLISN AIGSMCVISH FVMNTIRTTD SMPGPPSDGD VNKMCSALIG TCFWLSWMES 

      1990       2000       2010       2020       2030       2040 
DLNLYKTCLR SIMKSMPVRW FRALKNGKWF QKWDCKGDAI PKDSRLGDSM ANIGNWIRAW 

      2050       2060       2070       2080       2090       2100 
ELIRDGNISE PFDAAAVEML TTSVDKSLSW KKILKTTGIP RLLNSDTDVI DQSILNVQID 


IVENQAWQN

P13615 in FASTA format

View entry in original UniProtKB/Swiss-Prot format
View entry in raw text format (no links)
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	BLAST submission on ExPASy/SIB or at NCBI (USA)		Sequence analysis tools: ProtParam, ProtScale, Compute pI/Mw, PeptideMass, PeptideCutter, Dotlet (Java)
	ScanProsite, MotifScan		Submit a homology modeling request to SWISS-MODEL
	NPSA Sequence analysis tools