ExPASy logo ExPASy Home page Site Map Search ExPASy Contact us Swiss-Prot
Notice: This page will be replaced with www.uniprot.org. Please send us your feedback!
Search for

UniProtKB/Swiss-Prot entry P12646


[Entry info] [Name and origin] [References] [Comments] [Cross-references] [Keywords] [Features] [Sequence] [Tools]

Note: most headings are clickable, even if they don't appear as links. They link to the user manual or other documents.
Entry information
Entry name G6PD_DROME
Primary accession number P12646
Secondary accession numbers Q27574 Q27872 Q27879 Q27881 Q9VWE2 Q9VWE3
Integrated into Swiss-Prot on October 1, 1989
Sequence was last modified on July 15, 1998 (Sequence version 2)
Annotations were last modified on    July 22, 2008 (Entry version 90)
Name and origin of the protein
Protein name Glucose-6-phosphate 1-dehydrogenase
Synonyms G6PD
EC 1.1.1.49
Zwischenferment
Gene name
Name: Zw
Synonyms: G6PD
ORFNames: CG12529
From
Drosophila melanogaster (Fruit fly) [TaxID: 7227] 
Taxonomy Eukaryota; Metazoa; Arthropoda; Hexapoda; Insecta; Pterygota; Neoptera; Endopterygota; Diptera; Brachycera; Muscomorpha; Ephydroidea; Drosophilidae; Drosophila; Sophophora.
Protein existence 1: Evidence at protein level;
References
[1]
NUCLEOTIDE SEQUENCE (ISOFORM A).
DOI=10.1016/0378-1119(88)90530-6; PubMed=2838391 [NCBI, ExPASy, EBI, Israel, Japan]
Fouts D., Ganguly R., Gutierrez A.G., Lucchesi J.C., Manning J.E.;
"Nucleotide sequence of the Drosophila glucose-6-phosphate dehydrogenase gene and comparison with the homologous human gene.";
Gene 63:261-275(1988).
[2]
NUCLEOTIDE SEQUENCE (ISOFORM A).
STRAIN=F23.3, F24.1, MT32, MT41, MT68, Z11, Z16, Z21, Z27, Z3, Z41, Z42, Z5, Z55, Z62, Z64, and Z74;
PubMed=8913747 [NCBI, ExPASy, EBI, Israel, Japan]
Eanes W.F., Kirchner M., Yoon J., Biermann C.H., Wang I.N., McCartney M.A., Verrelli B.C.;
"Historical selection, amino acid polymorphism and lineage-specific divergence at the G6pd locus in Drosophila melanogaster and D. simulans.";
Genetics 144:1027-1041(1996).
[3]
NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
STRAIN=Berkeley;
DOI=10.1126/science.287.5461.2185; PubMed=10731132 [NCBI, ExPASy, EBI, Israel, Japan]
Adams M.D., Celniker S.E., Holt R.A., Evans C.A., Gocayne J.D., Amanatides P.G., Scherer S.E., Li P.W., Hoskins R.A., Galle R.F., George R.A., Lewis S.E., Richards S., Ashburner M., Henderson S.N., Sutton G.G., Wortman J.R., Yandell M.D., Zhang Q., Chen L.X., Brandon R.C., Rogers Y.-H.C., Blazej R.G., Champe M., Pfeiffer B.D., Wan K.H., Doyle C., Baxter E.G., Helt G., Nelson C.R., Miklos G.L.G., Abril J.F., Agbayani A., An H.-J., Andrews-Pfannkoch C., Baldwin D., Ballew R.M., Basu A., Baxendale J., Bayraktaroglu L., Beasley E.M., Beeson K.Y., Benos P.V., Berman B.P., Bhandari D., Bolshakov S., Borkova D., Botchan M.R., Bouck J., Brokstein P., Brottier P., Burtis K.C., Busam D.A., Butler H., Cadieu E., Center A., Chandra I., Cherry J.M., Cawley S., Dahlke C., Davenport L.B., Davies P., de Pablos B., Delcher A., Deng Z., Mays A.D., Dew I., Dietz S.M., Dodson K., Doup L.E., Downes M., Dugan-Rocha S., Dunkov B.C., Dunn P., Durbin K.J., Evangelista C.C., Ferraz C., Ferriera S., Fleischmann W., Fosler C., Gabrielian A.E., Garg N.S., Gelbart W.M., Glasser K., Glodek A., Gong F., Gorrell J.H., Gu Z., Guan P., Harris M., Harris N.L., Harvey D.A., Heiman T.J., Hernandez J.R., Houck J., Hostin D., Houston K.A., Howland T.J., Wei M.-H., Ibegwam C., Jalali M., Kalush F., Karpen G.H., Ke Z., Kennison J.A., Ketchum K.A., Kimmel B.E., Kodira C.D., Kraft C.L., Kravitz S., Kulp D., Lai Z., Lasko P., Lei Y., Levitsky A.A., Li J.H., Li Z., Liang Y., Lin X., Liu X., Mattei B., McIntosh T.C., McLeod M.P., McPherson D., Merkulov G., Milshina N.V., Mobarry C., Morris J., Moshrefi A., Mount S.M., Moy M., Murphy B., Murphy L., Muzny D.M., Nelson D.L., Nelson D.R., Nelson K.A., Nixon K., Nusskern D.R., Pacleb J.M., Palazzolo M., Pittman G.S., Pan S., Pollard J., Puri V., Reese M.G., Reinert K., Remington K., Saunders R.D.C., Scheeler F., Shen H., Shue B.C., Siden-Kiamos I., Simpson M., Skupski M.P., Smith T.J., Spier E., Spradling A.C., Stapleton M., Strong R., Sun E., Svirskas R., Tector C., Turner R., Venter E., Wang A.H., Wang X., Wang Z.-Y., Wassarman D.A., Weinstock G.M., Weissenbach J., Williams S.M., Woodage T., Worley K.C., Wu D., Yang S., Yao Q.A., Ye J., Yeh R.-F., Zaveri J.S., Zhan M., Zhang G., Zhao Q., Zheng L., Zheng X.H., Zhong F.N., Zhong W., Zhou X., Zhu S.C., Zhu X., Smith H.O., Gibbs R.A., Myers E.W., Rubin G.M., Venter J.C.;
"The genome sequence of Drosophila melanogaster.";
Science 287:2185-2195(2000).
[4]
GENOME REANNOTATION, AND ALTERNATIVE SPLICING.
PubMed=12537572 [NCBI, ExPASy, EBI, Israel, Japan]
Misra S., Crosby M.A., Mungall C.J., Matthews B.B., Campbell K.S., Hradecky P., Huang Y., Kaminker J.S., Millburn G.H., Prochnik S.E., Smith C.D., Tupy J.L., Whitfield E.J., Bayraktaroglu L., Berman B.P., Bettencourt B.R., Celniker S.E., de Grey A.D.N.J., Drysdale R.A., Harris N.L., Richter J., Russo S., Schroeder A.J., Shu S.Q., Stapleton M., Yamada C., Ashburner M., Gelbart W.M., Rubin G.M., Lewis S.E.;
"Annotation of the Drosophila melanogaster euchromatic genome: a systematic review.";
Genome Biol. 3:RESEARCH0083.1-RESEARCH0083.22(2002).
[5]
NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM A).
STRAIN=Berkeley;
TISSUE=Embryo;
PubMed=12537569 [NCBI, ExPASy, EBI, Israel, Japan]
Stapleton M., Carlson J.W., Brokstein P., Yu C., Champe M., George R.A., Guarin H., Kronmiller B., Pacleb J.M., Park S., Wan K.H., Rubin G.M., Celniker S.E.;
"A Drosophila full-length cDNA resource.";
Genome Biol. 3:RESEARCH0080.1-RESEARCH0080.8(2002).
[6]
PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-20, AND MASS SPECTROMETRY.
TISSUE=Embryo;
DOI=10.1021/pr700696a; PubMed=18327897 [NCBI, ExPASy, EBI, Israel, Japan]
Zhai B., Villen J., Beausoleil S.A., Mintseris J., Gygi S.P.;
"Phosphoproteome analysis of Drosophila melanogaster embryos.";
J. Proteome Res. 7:1675-1682(2008).
Comments
Copyright
Copyrighted by the UniProt Consortium, see http://www.uniprot.org/terms. Distributed under the Creative Commons Attribution-NoDerivs License.
Cross-references
Sequence databases
EMBL
M26674; AAA51463.1; -; Genomic_DNA.[EMBL / GenBank / DDBJ] [CoDingSequence]
M26673; AAA51463.1; JOINED; Genomic_DNA.[EMBL / GenBank / DDBJ] [CoDingSequence]
U42738; AAB02801.1; -; Genomic_DNA.[EMBL / GenBank / DDBJ] [CoDingSequence]
U42739; AAB02802.1; -; Genomic_DNA.[EMBL / GenBank / DDBJ] [CoDingSequence]
U42740; AAB02803.1; -; Genomic_DNA.[EMBL / GenBank / DDBJ] [CoDingSequence]
U42741; AAB02804.1; -; Genomic_DNA.[EMBL / GenBank / DDBJ] [CoDingSequence]
U42742; AAB02805.1; -; Genomic_DNA.[EMBL / GenBank / DDBJ] [CoDingSequence]
U42743; AAB02806.1; -; Genomic_DNA.[EMBL / GenBank / DDBJ] [CoDingSequence]
U42744; AAB02807.1; -; Genomic_DNA.[EMBL / GenBank / DDBJ] [CoDingSequence]
U42745; AAB02808.1; -; Genomic_DNA.[EMBL / GenBank / DDBJ] [CoDingSequence]
U42746; AAB02809.1; -; Genomic_DNA.[EMBL / GenBank / DDBJ] [CoDingSequence]
U42747; AAB02810.1; -; Genomic_DNA.[EMBL / GenBank / DDBJ] [CoDingSequence]
U42748; AAB02811.1; -; Genomic_DNA.[EMBL / GenBank / DDBJ] [CoDingSequence]
U42749; AAB02812.1; -; Genomic_DNA.[EMBL / GenBank / DDBJ] [CoDingSequence]
U43165; AAA99071.1; -; Genomic_DNA.[EMBL / GenBank / DDBJ] [CoDingSequence]
U43166; AAA99072.1; -; Genomic_DNA.[EMBL / GenBank / DDBJ] [CoDingSequence]
U43167; AAA99073.1; -; Genomic_DNA.[EMBL / GenBank / DDBJ] [CoDingSequence]
U44721; AAA99092.1; -; Genomic_DNA.[EMBL / GenBank / DDBJ] [CoDingSequence]
U45985; AAA99107.1; -; Genomic_DNA.[EMBL / GenBank / DDBJ] [CoDingSequence]
AE014298; AAF48999.1; -; Genomic_DNA.[EMBL / GenBank / DDBJ] [CoDingSequence]
AE014298; AAF49000.2; -; Genomic_DNA.[EMBL / GenBank / DDBJ] [CoDingSequence]
AY052079; AAK93503.1; -; mRNA.[EMBL / GenBank / DDBJ] [CoDingSequence]
PIR A47740; A47740.
JT0272; DEFFG6.
RefSeq NP_523411.1; -.
NP_728287.1; -.
UniGene Dm.225
3D structure databases
HSSP P11413; 1QKI. [HSSP ENTRY / PDB]
ModBase P12646.
Protein-protein interaction databases
DIP DIP:20748N; -.
IntAct P12646; -.
Organism-specific databases
FlyBase FBgn0004057; Zw.
Gene expression databases
ArrayExpress P12646; -.
GermOnline CG12529; Drosophila melanogaster.
Ontologies
GO
GO:0004345; Molecular function: glucose-6-phosphate dehydrogenase activity (traceable author statement from FlyBase).
GO:0005515; Molecular function: protein binding (inferred from physical interaction from IntAct).
QuickGo view.
Family and domain databases
InterPro IPR001282; Glc-6-P_DHase.
IPR016040; NAD(P)-bd.
Graphical view of domain structure.
Gene3D G3DSA:3.40.50.720; NAD(P)-bd; 1.
PANTHER PTHR23429; G6PDH; 1.
Pfam PF02781; G6PD_C; 1.
PF00479; G6PD_N; 1.
Pfam graphical view of domain structure.
PIRSF PIRSF000110; G6PD; 1.
PRINTS PR00079; G6PDHDRGNASE.
ProDom PD001129; G6PD; 1.
[Domain structure / List of seq. sharing at least 1 domain]
TIGRFAMs TIGR00871; zwf; 1.
PROSITE PS00069; G6P_DEHYDROGENASE; 1.
BLOCKS P12646.
Genome annotation databases
Ensembl CG12529; Drosophila melanogaster. [Contig view]
GeneID 32974; -.
KEGG dme:Dmel_CG12529; -.
Other
ProtoNet P12646.
UniRef View cluster of proteins with at least 50% / 90% / 100% identity.
Keywords
Alternative splicing; Carbohydrate metabolism; Complete proteome; Glucose metabolism; NADP; Oxidoreductase; Phosphoprotein; Polymorphism.
Features
SEVIEWER logo Feature table viewer
KeyFrom   To Length Description FTId
CHAIN   1   524  524     Glucose-6-phosphate 1-dehydrogenase. PRO_0000068092
ACT_SITE   267   267        Proton acceptor (By similarity). 
BINDING   44    44        NADP (By similarity). 
BINDING   76    76        NADP (By similarity). 
BINDING   205   205        Substrate (By similarity). 
BINDING   209   209        Substrate (By similarity). 
MOD_RES   20    20        Phosphoserine. 
VAR_SEQ   1    22        Missing (in isoform B). VSP_001593
VARIANT   32    32  1     G -> C (in strain: F24.1, MT32 and MT68). 
VARIANT   80    80  1     T -> N (in strain: Z74). 
VARIANT   382   382  1     L -> P (in strain: F23.3, MT41, Z3, Z5, Z11, Z16, Z21, Z27, Z42, Z55, Z64, Z74 and Berkeley). 
CONFLICT   185   186        QA -> AG (in Ref. 1; AAA51463). 
CONFLICT   214   214        N -> K (in Ref. 1; AAA51463). 
CONFLICT   344   346        LGV -> ARS (in Ref. 1; AAA51463). 
CONFLICT   461   465        DELRE -> AAAQ (in Ref. 1; AAA51463). 
Sequence information
Length: 524 AA [This is the length of the unprocessed precursor] Molecular weight: 60431 Da [This is the MW of the unprocessed precursor] CRC64: ABF81B763A82F1FD [This is a checksum on the sequence]
        10         20         30         40         50         60 
MATQKEDHTA LDLIIKSLKS PTMVCEGTHF DGKIPHTFVI FGASGDLAKK KIYPTLWWLY 

        70         80         90        100        110        120 
RDDLLPKPTK FCGYARSMLT VDSIKEQCLP YMKVQPHEQK KYEEFWALNE YVSGRYDGRT 

       130        140        150        160        170        180 
GFELLNQQLE IMENKNKANR IFYLALPPSV FEEVTVNIKQ ICMSVCGWNR VIIEKPFGRD 

       190        200        210        220        230        240 
DASSQALSDH LAGLFQEDQL YRIDHYLGKE MVQNLMTIRF GNKILSSTWN RENIASVLIT 

       250        260        270        280        290        300 
FKEPFGTQGR GGYFDEFGII RDVMQNHLLQ ILSLVAMEKP VSCHPDDIRD EKVKVLKSIE 

       310        320        330        340        350        360 
ALTLDDMVLG QYLGNPQGTN DDARTGYVED PTVSNDSNTP TYALGVLKIN NERWQGVPFI 

       370        380        390        400        410        420 
LRCGKALNER KAEVRIQYQD VLGDIFEGNT KRNELVIRVQ PGEALYFKMM TKSPGITFDI 

       430        440        450        460        470        480 
EETELDLTYE HRYKDSYLPD AYERLILDVF CGSQMHFVRS DELREAWRIF TPILHQIEKE 

       490        500        510        520 
HIRPITYQYG SRGPKEADRK CEENNFKYSG SYKWHGGKAA TSNH 

P12646 in FASTA format

View entry in original UniProtKB/Swiss-Prot format
View entry in raw text format (no links)
Report form for errors/updates in this UniProtKB/Swiss-Prot entry

BLAST logo BLAST submission on ExPASy/SIB
or at NCBI (USA)
Tools Sequence analysis tools: ProtParam, ProtScale, Compute pI/Mw, PeptideMass, PeptideCutter, Dotlet (Java)
PROSITE logo ScanProsite, MotifScan SWISS-MODEL Submit a homology modeling request to SWISS-MODEL
NPSA logo NPSA Sequence analysis tools

ExPASy logo ExPASy Home page Site Map Search ExPASy Contact us Swiss-Prot
 Hosted by ch flag SIB Switzerland Mirror sites: Australia  Brazil  Canada  China  Korea
Notice: This page will be replaced with www.uniprot.org. Please send us your feedback!