RNA-directed RNA polymerase
     (EC 2.7.7.48)
mRNA (guanine-N(7)-)-methyltransferase
     (EC 2.1.1.56)
mRNA guanylyltransferase
     (EC 2.7.7.-)

Gene name

Name:

From

Human parainfluenza 3 virus (strain Wash/47885/57) (HPIV-3) (Human parainfluenza 3 virus (strain NIH 47885))

[TaxID: 11217]

Taxonomy

Viruses; ssRNA negative-strand viruses; Mononegavirales; Paramyxoviridae; Paramyxovirinae; Respirovirus.

Virus host

Homo sapiens (Human) [TaxID: 9606]

Protein existence

2: Evidence at transcript level;

References

[1]	NUCLEOTIDE SEQUENCE. DOI=10.1016/0042-6822(88)90594-6; PubMed=2841798 [NCBI, ExPASy, EBI, Israel, Japan] Galinski M.S., Mink M.A., Pons M.W.; "Molecular cloning and sequence analysis of the human parainfluenza 3 virus gene encoding the L protein."; Virology 165:499-510(1988).
[2]	NUCLEOTIDE SEQUENCE [MRNA] OF 1-26. PubMed=2822598 [NCBI, ExPASy, EBI, Israel, Japan] Storey D.G., Cote M.-J., Dimock K., Kang C.Y.; "Nucleotide sequence of the coding and flanking regions of the human parainfluenza virus 3 hemagglutinin-neuraminidase gene: comparison with other paramyxoviruses."; Intervirology 27:69-80(1987).

Comments

FUNCTION: Displays RNA-directed RNA polymerase, mRNA guanylyl transferase, mRNA (guanine-N(7)-)-methyltransferase and poly(A) synthetase activities. The viral mRNA guanylyl transferase displays a different biochemical reaction than the cellular enzyme. The template is composed of the viral RNA tightly encapsidated by the nucleoprotein (N). Functions either as transcriptase or as replicase. The transcriptase synthesizes subsequently the subgenomic RNAs, assuring their capping and polyadenylation by a stuttering mechanism. The transcriptase stutters on a specific sequence, resulting on a cotranscriptional editing of the phosphoprotein (P) mRNA. The replicase mode is dependent on intracellular N protein concentration. In this mode, the polymerase replicates the whole viral genome without recognizing the transcriptional signals (By similarity).
CATALYTIC ACTIVITY: Nucleoside triphosphate + RNA(n) = diphosphate + RNA(n+1).
CATALYTIC ACTIVITY: S-adenosyl-L-methionine + G(5')pppR-RNA = S-adenosyl-L-homocysteine + m₇G(5')pppR-RNA.
SUBUNIT: Interacts with the P protein (By similarity).
SUBCELLULAR LOCATION: Virion (Potential). Cytoplasm (By similarity).
SIMILARITY: Belongs to the paramyxoviruses L protein family.
SIMILARITY: Contains 1 RdRp catalytic domain.

Copyright

Copyrighted by the UniProt Consortium, see http://www.uniprot.org/terms. Distributed under the Creative Commons Attribution-NoDerivs License.

Cross-references

Sequence databases

EMBL

M21649; AAA46854.1; -; Genomic_RNA.	[EMBL / GenBank / DDBJ] [CoDingSequence]
M20402; AAA46857.1; -; mRNA.	[EMBL / GenBank / DDBJ] [CoDingSequence]

PIR

B46451; B46451.

3D structure databases

ModBase

P12577.

Family and domain databases

InterPro

IPR016269; RNA-dir_RNA_pol_paramyxovir.
IPR014023; RNA_pol_cat.
IPR001016; RNA_pol_L_viral.
Graphical view of domain structure.

Pfam

PF00946; Paramyx_RNA_pol; 1.
Pfam graphical view of domain structure.

PIRSF

PIRSF000830; RNA_pol_ParamyxoV; 1.

PROSITE

PS50526; RDRP_SSRNA_NEG_NONSEG; 1.
PROSITE graphical view of domain structure (profiles).

Other

View cluster of proteins with at least 50% / 90% / 100% identity.

Keywords

ATP-binding; Cytoplasm; Methyltransferase; mRNA capping; mRNA processing; Multifunctional enzyme; Nucleotide-binding; Nucleotidyltransferase; RNA replication; RNA-directed RNA polymerase; S-adenosyl-L-methionine; Transferase; Virion.

Features

Feature table viewer

Feature aligner

`Key`	`From To`	`Length`	`Description`	`FTId`
`CHAIN`	`1 2233`	`2233`	`Large structural protein.`	`PRO_0000142733`
`DOMAIN`	`656 840`	`185`	`RdRp catalytic.`
`NP_BIND`	`1805 1814`	`10`	`ATP (Potential).`
`CONFLICT`	`26 26`		`K -> R (in Ref. 2; AAA46857).`

Sequence information

Length: 2233 AA [This is the length of the unprocessed precursor]

Molecular weight: 255806 Da [This is the MW of the unprocessed precursor]

CRC64: DBBB8B5DE74B4638 [This is a checksum on the sequence]

        10         20         30         40         50         60 
MDTESNNGTV SDILYPECHL NSPIVKGKIA QLHTIMSLPQ PYDMDDDSIL VITRQKIKLN 

        70         80         90        100        110        120 
KLDKRQRSIR RLKLILTEKV NDLGKYTFIR YPEMSKEMFK LHIPGINSKV TELLLKADRT 

       130        140        150        160        170        180 
YSQMTDGLRD LWINVLSKLA SKNDGSNYDL NEEINNISKV HTTYKSDKWY NPFKTWFTIK 

       190        200        210        220        230        240 
YDMRRLQKAR NEVTFNMGKD YNLLEDQKNF LLIHPELVLI LDKQNYNGYL ITPELVLPYC 

       250        260        270        280        290        300 
DVVEGRWNIS ACAKLDPKLQ SMYQKGNNLW EVIDKLFPIM GEKTFDVISL LEPLALSLIQ 

       310        320        330        340        350        360 
THDPVKQLRG AFLNHVLSEM ELIFESRESI KEFLSVDYID KILDIFNKST IDEIAEIFSF 

       370        380        390        400        410        420 
FRTFGHPPLE ASIAAEKVRK YMYIGKQLKF DTINKCHAIF CTIIINGYRE RHGGQWPPVT 

       430        440        450        460        470        480 
LPDHAHEFII NAYGSNSAIS YENAVDYYQS FIGIKFNKFI EPQLDEDLTI YMKDKALSPK 

       490        500        510        520        530        540 
KSNWDTVSPA SNLLYRTNAS NESRRLVEKF IADSKFDPNQ ILDYVESGDW LDDPEFNISY 

       550        560        570        580        590        600 
SLKEKEIKQE GRLFAKMTYK MRATQVLSET LLANNIGKFF QENGMVKGEI ELLKRLTTIS 

       610        620        630        640        650        660 
ISGVPRYNEV YNNSKSHTDD LKTYNKISNL NLSSNQKSKK FEFKSTDIYN DGYETVSCFL 

       670        680        690        700        710        720 
TTDLKKYCLN WRYESTALFG ETCNQIFGLN KLFNWLHPRL EGSTIYVGDP YCPPSDKEHI 

       730        740        750        760        770        780 
SLEDHPDSGF YVHNPRGGIE GFCQKLWTLI SISAIHLAAV RIGVRVTAMV QGDNQAIAVT 

       790        800        810        820        830        840 
TRVPNNYDYR VKKEIVYKDV VRFFDSLREV MDDLGHELKL NETIISSKMF IYSKRIYYDG 

       850        860        870        880        890        900 
RILPQALKAL SRCVFWSETV IDETRSASSN LATSFAKAIE NGYSPVLGYA CSIFKNIQQL 

       910        920        930        940        950        960 
YIALGMNINP TITQNIKDLY FRNPNWMQYA SLIPASVGGF NYMAMSRCFV RNIGDPSVAA 

       970        980        990       1000       1010       1020 
LADIKRFIKA NLLDRSVLYR IMNQEPGESS FLDWASDPYS CNLPQSQNIT TMIKNITARN 

      1030       1040       1050       1060       1070       1080 
VLQDSPNPLL SGLFTNTMIE EDEELAEFLM DRKVILPRVA HDILDNSLTG IRNAIAGMLD 

      1090       1100       1110       1120       1130       1140 
TTKSLIRVGI NRGGLTYSLL RKISNYDLVQ YETLSRTLRL IVSDKIRYED MCSVDLAIAL 

      1150       1160       1170       1180       1190       1200 
RQKMWIHLSG GRMISGLETP DPLELLSGVI ITGSEHCKIC YSSDGTNPYT WMYLPGNIKI 

      1210       1220       1230       1240       1250       1260 
GSAETGISSL RVPYFGSVTD ERSEAQLGYI KNLSKPAKAA IRIAMIYTWA FGNDEISWME 

      1270       1280       1290       1300       1310       1320 
ASQIAQTRAN FTLDSLKILT PVATSTNLSH RLKDTATQMK FSSTSLIRVS RFITMSNDNM 

      1330       1340       1350       1360       1370       1380 
SIKEANETKD TNLIYQQIML TGLSVFEYLF RLEETTGHNP IVMHLHIEDE CCIKESFNDE 

      1390       1400       1410       1420       1430       1440 
HINPESTLEL IRYPESNEFI YDKDPLKDVD LSKLMVIKDH SYTIDMNYWD DTDIIHAISI 

      1450       1460       1470       1480       1490       1500 
CTAITIADTM SQLDRDNLKE IIVIANDDDI NSLITEFLTL DILVFLKTFG GLLVNQFAYT 

      1510       1520       1530       1540       1550       1560 
LYSLKTEGRD LIWDYIMRTL RDTSHSILKV LSNALSHPKV FKRFWDCGVL NPIYGPNTAS 

      1570       1580       1590       1600       1610       1620 
QDQIKLALSI CEYSLDLFMR EWLNGVSLEI YICDSDMEVA NDRKQAFISR HLSFVCCLAE 

      1630       1640       1650       1660       1670       1680 
IASFGPNLLN LTYLERLDLL KQYLELNIKD DPTLKYVQIS GLLIKSFPST VTYVRKTAIK 

      1690       1700       1710       1720       1730       1740 
YLRIRGISPP EVIDDWDPIE DENMLDNIVK TINDNCNKDN KGNKINNFWG LALKNYQVLK 

      1750       1760       1770       1780       1790       1800 
IRSITSDSDN NDRSDASTGG LTLPQGGNYL SHQLRLFGIN STSCLKALEL SQILMKEVNK 

      1810       1820       1830       1840       1850       1860 
DQDRLFLGEG AGAMLACYDA TLGPAVNYYN SGLNITDVIG QRELKIFPSE VSLVGKKLGN 

      1870       1880       1890       1900       1910       1920 
VTQILNRVKV LFNGNPNSTW IGNMECETLI WSELNDKSIG LVHCDMEGAI GKSEETVLHE 

      1930       1940       1950       1960       1970       1980 
HYSVIRITYL IGDDDVVLIS KIIPTITPNW SRILYLYKLY WKDVSIISLK TSNPASTELY 

      1990       2000       2010       2020       2030       2040 
LISKDAYCTI MEPSEVVLSK LKRLSLLEEN NLLKWIILSK KKNNEWLHHE IKEGERDYGV 

      2050       2060       2070       2080       2090       2100 
MRPYHMALQI FGFQINLNHL AKEFLSTPDL TNINNIIQSF QRTIKDVLFE WINITHDGKR 

      2110       2120       2130       2140       2150       2160 
HKLGGRYNIF PLKNKGKLRL LSRRLVLSWI SLSLSTRLLT GRFPDEKFEH RAQTGYVSLP 

      2170       2180       2190       2200       2210       2220 
DTDLESLKLL SKNTIKNYRE CIGSISYWFL TKEVKILMKL IGGAKLLGIP RQYKEPEEQL 

      2230 
LEDYNQHDEF DID

P12577 in FASTA format

View entry in original UniProtKB/Swiss-Prot format
View entry in raw text format (no links)
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	BLAST submission on ExPASy/SIB or at NCBI (USA)		Sequence analysis tools: ProtParam, ProtScale, Compute pI/Mw, PeptideMass, PeptideCutter, Dotlet (Java)
	ScanProsite, MotifScan		Submit a homology modeling request to SWISS-MODEL
	NPSA Sequence analysis tools