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UniProtKB/Swiss-Prot entry P12527

[Entry info] [Name and origin] [References] [Comments] [Cross-references] [Keywords] [Features] [Sequence] [Tools]

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Entry information
Entry name LOX5_RAT
Primary accession number P12527
Secondary accession numbers None
Integrated into Swiss-Prot on October 1, 1989
Sequence was last modified on January 23, 2007 (Sequence version 3)
Annotations were last modified on    November 4, 2008 (Entry version 83)
Name and origin of the protein
Protein name Arachidonate 5-lipoxygenase
Synonyms 5-lipoxygenase
5-LO
EC 1.13.11.34
Gene name
Name: Alox5
From
Rattus norvegicus (Rat) [TaxID: 10116] 
Taxonomy Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Sciurognathi; Muroidea; Muridae; Murinae; Rattus.
Protein existence 2: Evidence at transcript level;
References
[1]
 NUCLEOTIDE SEQUENCE [MRNA].
PubMed=3417684 [NCBI, ExPASy, EBI, Israel, Japan]
Balcarek J.M., Theisen T.W., Cook M.N., Varrichio A., Hwang S.-M., Strohsacker M.W., Crooke S.T.;
"Isolation and characterization of a cDNA clone encoding rat 5-lipoxygenase.";
J. Biol. Chem. 263:13937-13941(1988).
[2]
 SEQUENCE REVISION TO 667-670.
DOI=10.1021/bi00076a003; PubMed=8518276 [NCBI, ExPASy, EBI, Israel, Japan]
Minor W., Steczko J., Bolin J.T., Otwinowski Z., Axelrod B.;
"Crystallographic determination of the active site iron and its ligands in soybean lipoxygenase L-1.";
Biochemistry 32:6320-6323(1993).
Comments
Copyright
Copyrighted by the UniProt Consortium, see http://www.uniprot.org/terms. Distributed under the Creative Commons Attribution-NoDerivs License.
Cross-references
Sequence databases
EMBL
J03960; AAA41538.1; ALT_FRAME; mRNA.[EMBL / GenBank / DDBJ] [CoDingSequence]
PIR A30882; A30882.
RefSeq NP_036954.1; -.
UniGene Rn.9662
3D structure databases
HSSP P12530; 1LOX. [HSSP ENTRY / PDB]
ModBase P12527.
Organism-specific databases
RGD 2096; Alox5.
Gene expression databases
ArrayExpress P12527; -.
GermOnline ENSRNOG00000012972; Rattus norvegicus.
Ontologies
GO
GO:0005737; Cellular component: cytoplasm (inferred from electronic annotation from UniProtKB-KW).
GO:0016020; Cellular component: membrane (inferred from electronic annotation from UniProtKB-KW).
GO:0004051; Molecular function: arachidonate 5-lipoxygenase activity (inferred from electronic annotation from EC).
GO:0005509; Molecular function: calcium ion binding (inferred from electronic annotation from UniProtKB-KW).
GO:0019370; Biological process: leukotriene biosynthetic process (inferred from electronic annotation from UniProtKB-KW).
GO:0055114; Biological process: oxidation reduction (inferred from electronic annotation from UniProtKB-KW).
QuickGo view.
Family and domain databases
InterPro IPR000907; LipOase.
IPR013819; LipOase_C.
IPR001024; LipOase_LH2.
IPR001885; LipOase_mml.
Graphical view of domain structure.
Gene3D G3DSA:2.60.60.20; Lipase_LipOase; 1.
PANTHER PTHR11771; LipOase; 1.
Pfam PF00305; Lipoxygenase; 1.
PF01477; PLAT; 1.
Pfam graphical view of domain structure.
PRINTS PR00087; LIPOXYGENASE.
PR00467; MAMLPOXGNASE.
SMART SM00308; LH2; 1.
SMART graphical view of domain structure.
PROSITE PS00711; LIPOXYGENASE_1; 1.
PS00081; LIPOXYGENASE_2; 1.
PS51393; LIPOXYGENASE_3; 1.
PS50095; PLAT; 1.
PROSITE graphical view of domain structure (profiles).
BLOCKS P12527.
ProtoNet P12527.
Genome annotation databases
Ensembl ENSRNOG00000012972; Rattus norvegicus. [Contig view]
GeneID 25290; -.
KEGG rno:25290; -.
Phylogenomic databases
HOVERGEN P12527; -.
Other
NextBio 606033; -.
UniRef View cluster of proteins with at least 50% / 90% / 100% identity.
Keywords
Calcium; Cytoplasm; Dioxygenase; Iron; Leukotriene biosynthesis; Membrane; Metal-binding; Oxidoreductase; Phosphoprotein.
Features
SEVIEWER logo Feature table viewer FT aligner logo Feature aligner
KeyFrom   To Length Description FTId
INIT_MET   1     1        Removed. 
CHAIN   2   673  672     Arachidonate 5-lipoxygenase. PRO_0000220696
DOMAIN   2   117  116     PLAT. 
DOMAIN   118   673  556     Lipoxygenase. 
METAL   17    17        Calcium 1; via carbonyl oxygen; structural (By similarity). 
METAL   18    18        Calcium 2; via carbonyl oxygen; structural (By similarity). 
METAL   19    19        Calcium 2; structural (By similarity). 
METAL   44    44        Calcium 2; structural (By similarity). 
METAL   45    45        Calcium 2; via carbonyl oxygen; structural (By similarity). 
METAL   47    47        Calcium 2; structural (By similarity). 
METAL   79    79        Calcium 1; via carbonyl oxygen; structural (By similarity). 
METAL   80    80        Calcium 1; via carbonyl oxygen; structural (By similarity). 
METAL   367   367        Iron; catalytic (By similarity). 
METAL   372   372        Iron; catalytic (By similarity). 
METAL   550   550        Iron; catalytic (By similarity). 
METAL   554   554        Iron; catalytic (By similarity). 
METAL   673   673        Iron; via carboxylate; catalytic (By similarity). 
MOD_RES   271   271        Phosphoserine (By similarity). 
MOD_RES   523   523        Phosphoserine (By similarity). 
Sequence information
Length: 673 AA [This is the length of the unprocessed precursor] Molecular weight: 78087 Da [This is the MW of the unprocessed precursor] CRC64: 536B0BC27CB0A608 [This is a checksum on the sequence] 
        10         20         30         40         50         60 
MPSYTVTVAT GSQWFAGTDD YIYLSLIGSA GCSEKHLLDK AFYNDFERGG RDSYDVTVDE 

        70         80         90        100        110        120 
ELGEIYLVKI EKRKYRLHDD WYLKYITLKT PHDYIEFPCY RWITGEGEIV LRDGCAKLAR 

       130        140        150        160        170        180 
DDQIHILKQH RRKELETRQK QYRWMEWNPG FPLSIDAKCH KDLPRDIQFD SEKGVDFVLN 

       190        200        210        220        230        240 
YSKAMENLFI NRFMHMFQSS WHDFADFEKI FVKISNTISE RVKNHWQEDL MFGYQFLNGC 

       250        260        270        280        290        300 
NPVLIKRCTE LPKKLPVTTE MVECSLERQL SLEQEVQEGN IFIVDYELLD GIDANKTDPC 

       310        320        330        340        350        360 
THQFLAAPIC LLYKNLANKI VPIAIQLNQT PGEKNPIFLP TDSKYDWLLA KIWVRSSDFH 

       370        380        390        400        410        420 
IHQTITHLLR THLVSEVFGI AMYRQLPAVH PLFKLLVAHV RFTIAINTKA REQLNCEYGL 

       430        440        450        460        470        480 
FDKANATGGG GHVQMVQRAV QDLTYSSLCF PEAIKARGMD NTEDIPYYFY RDDGLLVWEA 

       490        500        510        520        530        540 
IQSFTTEVVS IYYEDDQVVE EDQELQDFVK DVYVYGMRGR KASGFPKSIK SREKLSEYLT 

       550        560        570        580        590        600 
VVIFTASAQH AAVNFGQYDW CSWIPNAPPT MRAPPPTAKG VVTIEQIVDT LPDRGRSCWH 

       610        620        630        640        650        660 
LGAVWALSQF QENELFLGMY PEEHFIEKPV KEAMIRFRKN LEAIVSVIAE RNKNKKLPYY 

       670 
YLSPDRIPNS VAI
P12527 in FASTA format

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