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UniProtKB/Swiss-Prot entry P11836


[Entry info] [Name and origin] [References] [Comments] [Cross-references] [Keywords] [Features] [Sequence] [Tools]

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Entry information
Entry name CD20_HUMAN
Primary accession number P11836
Secondary accession numbers A6NMS4 P08984 Q13963
Integrated into Swiss-Prot on October 1, 1989
Sequence was last modified on October 1, 1989 (Sequence version 1)
Annotations were last modified on    June 16, 2009 (Entry version 93)
Name and origin of the protein
Protein name B-lymphocyte antigen CD20
Synonyms Membrane-spanning 4-domains subfamily A member 1
B-lymphocyte surface antigen B1
Leu-16
Bp35
CD20 antigen
Gene name
Name: MS4A1
Synonyms: CD20
From
Homo sapiens (Human) [TaxID: 9606] 
Taxonomy Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini; Catarrhini; Hominidae; Homo.
Protein existence 1: Evidence at protein level;
References
[1]
NUCLEOTIDE SEQUENCE [MRNA].
DOI=10.1084/jem.167.6.1975; PubMed=3260267 [NCBI, ExPASy, EBI, Israel, Japan]
Stamenkovic I., Seed B.;
"Analysis of two cDNA clones encoding the B lymphocyte antigen CD20 (B1, Bp35), a type III integral membrane protein.";
J. Exp. Med. 167:1975-1980(1988).
[2]
NUCLEOTIDE SEQUENCE [MRNA].
DOI=10.1073/pnas.85.1.208; PubMed=2448768 [NCBI, ExPASy, EBI, Israel, Japan]
Tedder T.F., Streuli M., Schlossman S.F., Saito H.;
"Isolation and structure of a cDNA encoding the B1 (CD20) cell-surface antigen of human B lymphocytes.";
Proc. Natl. Acad. Sci. U.S.A. 85:208-212(1988).
[3]
NUCLEOTIDE SEQUENCE [MRNA].
PubMed=2456210 [NCBI, ExPASy, EBI, Israel, Japan]
Einfeld D.A., Brown J.P., Valentine M.A., Clark E.A., Ledbetter J.A.;
"Molecular cloning of the human B cell CD20 receptor predicts a hydrophobic protein with multiple transmembrane domains.";
EMBO J. 7:711-717(1988).
[4]
NUCLEOTIDE SEQUENCE [GENOMIC DNA].
PubMed=2466899 [NCBI, ExPASy, EBI, Israel, Japan]
Tedder T.F., Klejman G., Schlossman S.F., Saito H.;
"Structure of the gene encoding the human B lymphocyte differentiation antigen CD20 (B1).";
J. Immunol. 142:2560-2568(1989).
[5]
NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
DOI=10.1038/nature04632; PubMed=16554811 [NCBI, ExPASy, EBI, Israel, Japan]
Taylor T.D., Noguchi H., Totoki Y., Toyoda A., Kuroki Y., Dewar K., Lloyd C., Itoh T., Takeda T., Kim D.-W., She X., Barlow K.F., Bloom T., Bruford E., Chang J.L., Cuomo C.A., Eichler E., FitzGerald M.G., Jaffe D.B., LaButti K., Nicol R., Park H.-S., Seaman C., Sougnez C., Yang X., Zimmer A.R., Zody M.C., Birren B.W., Nusbaum C., Fujiyama A., Hattori M., Rogers J., Lander E.S., Sakaki Y.;
"Human chromosome 11 DNA sequence and analysis including novel gene identification.";
Nature 440:497-500(2006).
[6]
NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Mural R.J., Istrail S., Sutton G.G., Florea L., Halpern A.L., Mobarry C.M., Lippert R., Walenz B., Shatkay H., Dew I., Miller J.R., Flanigan M.J., Edwards N.J., Bolanos R., Fasulo D., Halldorsson B.V., Hannenhalli S., Turner R., Yooseph S., Lu F., Nusskern D.R., Shue B.C., Zheng X.H., Zhong F., Delcher A.L., Huson D.H., Kravitz S.A., Mouchard L., Reinert K., Remington K.A., Clark A.G., Waterman M.S., Eichler E.E., Adams M.D., Hunkapiller M.W., Myers E.W., Venter J.C.;
Submitted (JUL-2005) to the EMBL/GenBank/DDBJ databases.
[7]
NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
TISSUE=Lymph;
DOI=10.1101/gr.2596504; PubMed=15489334 [NCBI, ExPASy, EBI, Israel, Japan]
The MGC Project Team;
"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC).";
Genome Res. 14:2121-2127(2004).
[8]
EPITOPE MAPPING, AND MUTAGENESIS OF THR-159; ASN-163; ASN-166; ALA-170 AND PRO-172.
PubMed=16785532 [NCBI, ExPASy, EBI, Israel, Japan]
Teeling J.L., Mackus W.J.M., Wiegman L.J.J.M., van den Brakel J.H.N., Beers S.A., French R.R., van Meerten T., Ebeling S., Vink T., Slootstra J.W., Parren P.W.H.I., Glennie M.J., van de Winkel J.G.J.;
"The biological activity of human CD20 monoclonal antibodies is linked to unique epitopes on CD20.";
J. Immunol. 177:362-371(2006).
Comments
  • FUNCTION: This protein may be involved in the regulation of B-cell activation and proliferation.
  • SUBCELLULAR LOCATION: Membrane; Multi-pass membrane protein.
  • TISSUE SPECIFICITY: Expressed on B-cells.
  • PTM: Phosphorylated. Might be functionally regulated by protein kinase(s).
  • PHARMACEUTICAL: Monoclonal antibodies(mAb) against CD20 are used to treat B-cell non-Hodgkin's lymphoma (NHL). These antibodies include Rituximab (Mabthera), Britumomab (Zevalin) and Tositumomab (Bexxar). CD20 engaged by mAb can generate transmembrane signals capable of directly controlling cell growth and triggering cell death in certain tumors. Alternatively, mAb can mediate complement-dependent cytotoxicity.
  • SIMILARITY: Belongs to the MS4A family.
  • CAUTION: Epitope 1, mapped in PubMed:16785532, is predicted to be buried in the membrane. Its accessibility to the extracellular space, and thus to antibody recognition, is not explained.
  • WEB RESOURCE: Name=Wikipedia; Note=CD20 entry; URL="http://en.wikipedia.org/wiki/CD20";.
Copyright
Copyrighted by the UniProt Consortium, see http://www.uniprot.org/terms. Distributed under the Creative Commons Attribution-NoDerivs License.
Cross-references
Sequence databases
EMBL
X12530; CAA31046.1; -; mRNA.[EMBL / GenBank / DDBJ] [CoDingSequence]
M27394; AAA35581.1; -; Genomic_DNA.[EMBL / GenBank / DDBJ] [CoDingSequence]
X07203; CAA30179.1; -; mRNA.[EMBL / GenBank / DDBJ] [CoDingSequence]
X07204; CAA30180.1; -; mRNA.[EMBL / GenBank / DDBJ] [CoDingSequence]
L23419; AAA88911.1; -; Genomic_DNA.[EMBL / GenBank / DDBJ] [CoDingSequence]
L23415; AAA88911.1; JOINED; Genomic_DNA.[EMBL / GenBank / DDBJ] [CoDingSequence]
L23416; AAA88911.1; JOINED; Genomic_DNA.[EMBL / GenBank / DDBJ] [CoDingSequence]
L23417; AAA88911.1; JOINED; Genomic_DNA.[EMBL / GenBank / DDBJ] [CoDingSequence]
M27395; -; NOT_ANNOTATED_CDS; Genomic_DNA.[EMBL / GenBank / DDBJ]
AP003127; -; NOT_ANNOTATED_CDS; Genomic_DNA.[EMBL / GenBank / DDBJ]
CH471076; EAW73889.1; -; Genomic_DNA.[EMBL / GenBank / DDBJ] [CoDingSequence]
BC002807; AAH02807.1; -; mRNA.[EMBL / GenBank / DDBJ] [CoDingSequence]
IPI IPI00007880; -.
PIR A30586; A30586.
RefSeq NP_068769.2; -.
NP_690605.1; -.
UniGene Hs.712553
3D structure databases
PDB
1S8B; Model; -; A=1-297.[ExPASy / RCSB / EBI]
2OSL; X-ray; 2.60 A; P/Q=163-187.[ExPASy / RCSB / EBI]
3BKY; X-ray; 2.61 A; P=163-187.[ExPASy / RCSB / EBI]
Detailed list of linked structures.
PDBsum 1S8B; -.
2OSL; -.
3BKY; -.
ModBase P11836.
Protein family/group databases
TCDB 1.A.37.1.1; CD20 Ca2+ channel (CD20) family.
Organism-specific databases
GeneCards GC11P059979; -.
H-InvDB HIX0009670; -.
HGNC HGNC:7315; MS4A1.
GenAtlas MS4A1.
HPA CAB000015; -.
HPA014341; -.
HPA014391; -.
MIM 112210; gene. [NCBI / EBI]
PharmGKB PA31111; -.
Gene expression databases
ArrayExpress P11836; -.
Bgee P11836; -.
CleanEx HS_MS4A1; -.
GermOnline ENSG00000156738; Homo sapiens.
Ontologies
GO
GO:0005887; Cellular component: integral to plasma membrane (traceable author statement from ProtInc).
GO:0004872; Molecular function: receptor activity (inferred from electronic annotation from InterPro).
GO:0042113; Biological process: B cell activation (inferred from electronic annotation from UniProtKB-KW).
GO:0006955; Biological process: immune response (traceable author statement from ProtInc).
GO:0007165; Biological process: signal transduction (inferred from electronic annotation from InterPro).
QuickGo view.
Family and domain databases
InterPro IPR007237; CD20IgEFc_rcpt_B.
Graphical view of domain structure.
Pfam PF04103; CD20; 1.
Pfam graphical view of domain structure.
Proteomic databases
PRIDE P11836; -.
Genome annotation databases
Ensembl ENSG00000156738; Homo sapiens. [Contig view]
GeneID 931; -.
KEGG hsa:931; -.
Phylogenomic databases
HOGENOM P11836; -.
HOVERGEN P11836; -.
OMA P11836; FFMRESK.
Other
DrugBank DB00078; Ibritumomab.
DB00073; Rituximab.
DB00081; Tositumomab.
NextBio 3860; -.
SOURCE MS4A1; Homo sapiens.
ProtoNet P11836.
UniRef View cluster of proteins with at least 50% / 90% / 100% identity.
Keywords
3D-structure; B-cell activation; Disulfide bond; Membrane; Pharmaceutical; Phosphoprotein; Transmembrane.
Features
SEVIEWER logo Feature table viewer FT aligner logo Feature aligner
KeyFrom   To Length Description FTId
CHAIN   1   297  297     B-lymphocyte antigen CD20. PRO_0000158627
TOPO_DOM   1    63  63     Cytoplasmic (Potential). 
TRANSMEM   64    84  21     Potential. 
TRANSMEM   85   105  21     Potential. 
TRANSMEM   121   141  21     Potential. 
TRANSMEM   189   209  21     Potential. 
TOPO_DOM   210   297  88     Cytoplasmic (Potential). 
REGION   74    80  7     Epitope 1. 
REGION   146   160  15     Epitope 2. 
REGION   168   175  8     Epitope 3 (recognized by antibodies, including Rituximab). 
DISULFID   111   220        Probable. 
MUTAGEN   159   159        T->K: Abrogates recognition by some antibodies; when associated with D-163 and D-166. Slight decrease of rituximab binding; when associated with D-163 and D-166. 
MUTAGEN   163   163        N->D: Decreased binding of some antibodies. No effect on rituximab binding. 
MUTAGEN   166   166        N->D: Decreased binding of some antibodies. No effect on rituximab binding. 
MUTAGEN   170   170        A->S: Abrogates recognition by therapeutic antibodies, including rituximab; when associated with S-172. 
MUTAGEN   172   172        P->S: Marked reduction in rituximab binding. Abrogates recognition by antibodies, including rituximab; when associated with S-170. 
CONFLICT   13    13        P -> L (in Ref. 3; CAA30179/CAA30180). 
CONFLICT   71    71        M -> I (in Ref. 4; AAA88911). 
HELIX   172   174  3      
HELIX   178   184  7      
Sequence information
Length: 297 AA [This is the length of the unprocessed precursor] Molecular weight: 33077 Da [This is the MW of the unprocessed precursor] CRC64: AC5420F8B626BDD1 [This is a checksum on the sequence]
        10         20         30         40         50         60 
MTTPRNSVNG TFPAEPMKGP IAMQSGPKPL FRRMSSLVGP TQSFFMRESK TLGAVQIMNG 

        70         80         90        100        110        120 
LFHIALGGLL MIPAGIYAPI CVTVWYPLWG GIMYIISGSL LAATEKNSRK CLVKGKMIMN 

       130        140        150        160        170        180 
SLSLFAAISG MILSIMDILN IKISHFLKME SLNFIRAHTP YINIYNCEPA NPSEKNSPST 

       190        200        210        220        230        240 
QYCYSIQSLF LGILSVMLIF AFFQELVIAG IVENEWKRTC SRPKSNIVLL SAEEKKEQTI 

       250        260        270        280        290 
EIKEEVVGLT ETSSQPKNEE DIEIIPIQEE EEEETETNFP EPPQDQESSP IENDSSP 

P11836 in FASTA format

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