ID   L_NDVB                  Reviewed;        2204 AA.
AC   P11205;
DT   01-JUL-1989, integrated into UniProtKB/Swiss-Prot.
DT   01-JUL-1989, sequence version 1.
DT   25-NOV-2008, entry version 56.
DE   RecName: Full=Large structural protein;
DE            Short=Protein L;
DE   AltName: Full=Transcriptase;
DE   AltName: Full=Replicase;
DE   Includes:
DE     RecName: Full=RNA-directed RNA polymerase;
DE              EC=2.7.7.48;
DE   Includes:
DE     RecName: Full=mRNA (guanine-N(7)-)-methyltransferase;
DE              EC=2.1.1.56;
DE   Includes:
DE     RecName: Full=mRNA guanylyltransferase;
DE              EC=2.7.7.-;
GN   Name=L;
OS   Newcastle disease virus (strain Beaudette C/45) (NDV).
OC   Viruses; ssRNA negative-strand viruses; Mononegavirales;
OC   Paramyxoviridae; Paramyxovirinae; Avulavirus.
OX   NCBI_TaxID=11178;
OH   NCBI_TaxID=9031; Gallus gallus (Chicken).
RN   [1]
RP   NUCLEOTIDE SEQUENCE [GENOMIC RNA].
RX   MEDLINE=87230982; PubMed=3035486; DOI=10.1093/nar/15.10.3961;
RA   Yusoff K., Millar N.S., Chambers P., Emmerson P.T.;
RT   "Nucleotide sequence analysis of the L gene of Newcastle disease
RT   virus: homologies with Sendai and vesicular stomatitis viruses.";
RL   Nucleic Acids Res. 15:3961-3976(1987).
CC   -!- FUNCTION: Displays RNA-directed RNA polymerase, mRNA guanylyl
CC       transferase, mRNA (guanine-N(7)-)-methyltransferase and poly(A)
CC       synthetase activities. The viral mRNA guanylyl transferase
CC       displays a different biochemical reaction than the cellular
CC       enzyme. The template is composed of the viral RNA tightly
CC       encapsidated by the nucleoprotein (N). Functions either as
CC       transcriptase or as replicase. The transcriptase synthesizes
CC       subsequently the subgenomic RNAs, assuring their capping and
CC       polyadenylation by a stuttering mechanism. The transcriptase
CC       stutters on a specific sequence, resulting on a cotranscriptional
CC       editing of the phosphoprotein (P) mRNA. The replicase mode is
CC       dependent on intracellular N protein concentration. In this mode,
CC       the polymerase replicates the whole viral genome without
CC       recognizing the transcriptional signals (By similarity).
CC   -!- CATALYTIC ACTIVITY: Nucleoside triphosphate + RNA(n) = diphosphate
CC       + RNA(n+1).
CC   -!- CATALYTIC ACTIVITY: S-adenosyl-L-methionine + G(5')pppR-RNA = S-
CC       adenosyl-L-homocysteine + m(7)G(5')pppR-RNA.
CC   -!- SUBUNIT: Interacts with the P protein (By similarity).
CC   -!- SUBCELLULAR LOCATION: Virion (Potential). Cytoplasm (By
CC       similarity).
CC   -!- SIMILARITY: Belongs to the paramyxoviruses L protein family.
CC   -!- SIMILARITY: Contains 1 RdRp catalytic domain.
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DR   EMBL; X05399; CAA28985.1; -; Genomic_RNA.
DR   PIR; A26747; RRNZNV.
DR   GO; GO:0005737; C:cytoplasm; IEA:UniProtKB-KW.
DR   GO; GO:0019012; C:virion; IEA:UniProtKB-KW.
DR   GO; GO:0005524; F:ATP binding; IEA:UniProtKB-KW.
DR   GO; GO:0004482; F:mRNA (guanine-N7-)-methyltransferase activity; IEA:EC.
DR   GO; GO:0003723; F:RNA binding; IEA:InterPro.
DR   GO; GO:0003968; F:RNA-directed RNA polymerase activity; IEA:InterPro.
DR   GO; GO:0006370; P:mRNA capping; IEA:UniProtKB-KW.
DR   GO; GO:0006410; P:transcription, RNA-dependent; IEA:UniProtKB-KW.
DR   InterPro; IPR016269; RNA-dir_RNA_pol_paramyxovir.
DR   InterPro; IPR014023; RNA_pol_cat.
DR   InterPro; IPR001016; RNA_pol_L_viral.
DR   Pfam; PF00946; Paramyx_RNA_pol; 1.
DR   PIRSF; PIRSF000830; RNA_pol_ParamyxoV; 1.
DR   PROSITE; PS50526; RDRP_SSRNA_NEG_NONSEG; 1.
PE   3: Inferred from homology;
KW   ATP-binding; Cytoplasm; Methyltransferase; mRNA capping;
KW   mRNA processing; Multifunctional enzyme; Nucleotide-binding;
KW   Nucleotidyltransferase; RNA replication; RNA-directed RNA polymerase;
KW   S-adenosyl-L-methionine; Transferase; Virion.
FT   CHAIN         1   2204       Large structural protein.
FT                                /FTId=PRO_0000142731.
FT   DOMAIN      634    818       RdRp catalytic.
FT   NP_BIND    1775   1784       ATP (Potential).
SQ   SEQUENCE   2204 AA;  248824 MW;  C67B8674D904802C CRC64;
     MASSGPERAE HQIILPESHL SSPLVKHKLL YYWKLTGLPL PDECDFDHLI LSRQWKKILE
     SASPDTERMI KLGRAVHQTL NHNSRITGVL HPRCLEELAS IEVPDSTNKF RKIEKKIQIH
     NTRYGELFTR LCTHIEKKLL GSSWSNNVPR SEEFNSIRTD PAFWFHSKWS TAKFAWLHIK
     QIQRHLIVAA RTRSAANKLV MLTHKVGQVF VTPELVIVTH TNENKFTCLT QELVLMYADM
     MEGRDMVNII STTAVHLRSL SEKIDDILQL IDALAKDLGN QVYDVVSLME GFAYGAVQLL
     EPSGRFAGHF FAFNLQELKD ILIGLLPNDI AESVTHAIAT VFSGLEQNQA AEMLCLLRLW
     GHPLLESRIA AKAVRSQMCA PKMVDFDMIL QVLSFFKGTI INGYRKKNAG VWPRVKVDTI
     YGKIIGQLHA DSAEISHDIM LREYKSLSAL EFEPCIEYDP VTNLSMFLKD KAIAHPNDNW
     LASFRRNLLS EDQKKHVKEA TSTNRLLIEF LESNDFDPYK EMEYLTTLEY LRDDDVAVSY
     SLKEKEVKVN GRIFAKLTKK LRNCQVMAEG ILADQIAPFF QGNGVIQDSI SLTKSTLAMS
     QLSFNSNKKR ITDCKERVSS NRNHDPKSKN RRRVATFITT DLQKYCLNWR YQTIKLFAHA
     INQLMGLPHF FEWIHLRLMD TTMFVGDPFN PPSDPTDCDL SRVPNDDIYI VSARGGIEGL
     CQKLWTMISI AAIQLAAARS HCRVACMVQG DNQVIAVTRE VRSDDSPEMV LTQLHQASDN
     FFKELIHVNH LIGHNLKDRE TIRSDTFFIY SKRIFKDGAI LSQVLKNSSK LVMVSGDLSE
     NTVMSCANIA STVARLCENG LPKDFCYYLN YIMSCVQTYF DSEFSYNNNS HPDLNQSWIE
     DISFVHSYVL TPAQLGGLSN LQYSRLYTRN IGDPGTTAFA EIKRLEAVGL LSPNIMTNIL
     TRPPGNGDWA SLCNDPYSFN FETVASPNIV LKKHTQRVLF ETCSNPLLSG VHTEDNEAEE
     KALAEFLLNQ EVIHPRVAHA IMEASSVGRR KQIQGLVDTT NTVIKIALTR RPLGIKRLMR
     IVNYSSMHAM LFRDDVFSSN RSNHPLVSSN MCSLTLADYA RNRSWSPLTG GRKILGVSNP
     DTIELVEGEI LSVSGGCTRC DSGDEQFTWF HLPSNIELTD DTSKNPPMRV PYLGSKTQER
     RAASLAKIAH MSPHVKAALR ASSVLIWAYG DNEVNWTAAL TIAKSRCNIN LEYLRLLSPL
     PTAGNLQHRL DDGITQMTFT PASLYRCHLT FTYPMILKGY SLKKESKRGM WFINRVMLLG
     LSLIESIFPM TTTRTYDEIT LHLHSKFSCC IREAPVAVPF ELLGVAPELR TVTSNKFMYD
     PSPVSEGDFA RLDLAIFKSY ELNLESYPTI ELMNILSISS GKLIGQSVVS YDEDTSIKND
     AIIVYDNTRN WISEAQNSDV VRLFEYAALE VLLDCSYQLY YLRVRGLDNI VLYMGDLYKN
     MPGILLSNIA ATISHPVIHS RLHAVGLVNH NGSHQLADTD FIEMSAKLLV SCTRRVISGL
     YSGNKYDLLF PSVLDDNLNE KMLQLISRLC CLYTVLFATT REIPKIRGLS AEEKCSVLTE
     YLLSDAVKPL LSPDQVSSIM SPNIITFPAN LYYMSRKSLN LIREREDKDS ILALLFPQEP
     LLEFPSVQDI GARVKDPFTR QPAAFLQELD LSAPARYDAF TLSQIHPELT SPNPEEDYLV
     RYLFRGIGTA SSSWYKASHL LSVPEVRCAR HGNSLYLAEG SGAIMSLLEL HVPHETIYYN
     TLFSNEMNPP QRHFGPTPTQ FLNSVVYRNL QAEVTCKDGF VQEFRPLWRE NTEESDLTSD
     KAVGYITSAV PYRSVSLLHC DIEIPPGSNQ SLLDQLAINL SLIAMHSVRE GGVVIIKVLY
     AMGYYFHLLM NLFAPCSTKG YILSNGYACR GDMECYLVFV MGYLGGPTFV HEVVRMAKTL
     VQRHGTLLSK SDEITLTRLF TSQRQRVTDI LSSPLPRLIK YLRKNIDTAL IEAGGQPVRP
     FCAESLVSTL ADITQITQII ASHIDTVIRS VIYMEAEGDL ADTVFLFTPY NLSTDGKKRT
     SLKQCTRQIL EVTILGLRVE DLNKIGDVIS LVLKGMISME DLIPLRTYLK HSTCPKYLKA
     VLGITKLKEM FTDTSVLYLT RAQQKFYMKT IGNAVKGYYS NCDS
//