RNA-directed RNA polymerase
     (EC 2.7.7.48)
mRNA (guanine-N(7)-)-methyltransferase
     (EC 2.1.1.56)
mRNA guanylyltransferase
     (EC 2.7.7.-)

Gene name

Name:

From

Newcastle disease virus (strain Beaudette C/45) (NDV)

[TaxID: 11178]

Taxonomy

Viruses; ssRNA negative-strand viruses; Mononegavirales; Paramyxoviridae; Paramyxovirinae; Avulavirus.

Virus host

Gallus gallus (Chicken) [TaxID: 9031]

Protein existence

3: Inferred from homology;

References

[1]

NUCLEOTIDE SEQUENCE [GENOMIC RNA].
DOI=10.1093/nar/15.10.3961; PubMed=3035486 [NCBI, ExPASy, EBI, Israel, Japan]
Yusoff K., Millar N.S., Chambers P., Emmerson P.T.;
"Nucleotide sequence analysis of the L gene of Newcastle disease virus: homologies with Sendai and vesicular stomatitis viruses.";
Nucleic Acids Res. 15:3961-3976(1987).

Comments

FUNCTION: Displays RNA-directed RNA polymerase, mRNA guanylyl transferase, mRNA (guanine-N(7)-)-methyltransferase and poly(A) synthetase activities. The viral mRNA guanylyl transferase displays a different biochemical reaction than the cellular enzyme. The template is composed of the viral RNA tightly encapsidated by the nucleoprotein (N). Functions either as transcriptase or as replicase. The transcriptase synthesizes subsequently the subgenomic RNAs, assuring their capping and polyadenylation by a stuttering mechanism. The transcriptase stutters on a specific sequence, resulting on a cotranscriptional editing of the phosphoprotein (P) mRNA. The replicase mode is dependent on intracellular N protein concentration. In this mode, the polymerase replicates the whole viral genome without recognizing the transcriptional signals (By similarity).
CATALYTIC ACTIVITY: Nucleoside triphosphate + RNA(n) = diphosphate + RNA(n+1).
CATALYTIC ACTIVITY: S-adenosyl-L-methionine + G(5')pppR-RNA = S-adenosyl-L-homocysteine + m₇G(5')pppR-RNA.
SUBUNIT: Interacts with the P protein (By similarity).
SUBCELLULAR LOCATION: Virion (Potential). Cytoplasm (By similarity).
SIMILARITY: Belongs to the paramyxoviruses L protein family.
SIMILARITY: Contains 1 RdRp catalytic domain.

Copyright

Copyrighted by the UniProt Consortium, see http://www.uniprot.org/terms. Distributed under the Creative Commons Attribution-NoDerivs License.

Cross-references

Sequence databases

EMBL

X05399; CAA28985.1; -; Genomic_RNA.

[EMBL / GenBank / DDBJ] [CoDingSequence]

PIR

A26747; RRNZNV.

3D structure databases

ModBase

P11205.

Family and domain databases

InterPro

IPR016269; RNA-dir_RNA_pol_paramyxovir.
IPR014023; RNA_pol_cat.
IPR001016; RNA_pol_L_viral.
Graphical view of domain structure.

Pfam

PF00946; Paramyx_RNA_pol; 1.
Pfam graphical view of domain structure.

PIRSF

PIRSF000830; RNA_pol_ParamyxoV; 1.

PROSITE

PS50526; RDRP_SSRNA_NEG_NONSEG; 1.
PROSITE graphical view of domain structure (profiles).

Other

View cluster of proteins with at least 50% / 90% / 100% identity.

Keywords

ATP-binding; Cytoplasm; Methyltransferase; mRNA capping; mRNA processing; Multifunctional enzyme; Nucleotide-binding; Nucleotidyltransferase; RNA replication; RNA-directed RNA polymerase; S-adenosyl-L-methionine; Transferase; Virion.

Features

Feature table viewer

Feature aligner

`Key`	`From To`	`Length`	`Description`	`FTId`
`CHAIN`	`1 2204`	`2204`	`Large structural protein.`	`PRO_0000142731`
`DOMAIN`	`634 818`	`185`	`RdRp catalytic.`
`NP_BIND`	`1775 1784`	`10`	`ATP (Potential).`

Sequence information

Length: 2204 AA [This is the length of the unprocessed precursor]

Molecular weight: 248824 Da [This is the MW of the unprocessed precursor]

CRC64: C67B8674D904802C [This is a checksum on the sequence]

        10         20         30         40         50         60 
MASSGPERAE HQIILPESHL SSPLVKHKLL YYWKLTGLPL PDECDFDHLI LSRQWKKILE 

        70         80         90        100        110        120 
SASPDTERMI KLGRAVHQTL NHNSRITGVL HPRCLEELAS IEVPDSTNKF RKIEKKIQIH 

       130        140        150        160        170        180 
NTRYGELFTR LCTHIEKKLL GSSWSNNVPR SEEFNSIRTD PAFWFHSKWS TAKFAWLHIK 

       190        200        210        220        230        240 
QIQRHLIVAA RTRSAANKLV MLTHKVGQVF VTPELVIVTH TNENKFTCLT QELVLMYADM 

       250        260        270        280        290        300 
MEGRDMVNII STTAVHLRSL SEKIDDILQL IDALAKDLGN QVYDVVSLME GFAYGAVQLL 

       310        320        330        340        350        360 
EPSGRFAGHF FAFNLQELKD ILIGLLPNDI AESVTHAIAT VFSGLEQNQA AEMLCLLRLW 

       370        380        390        400        410        420 
GHPLLESRIA AKAVRSQMCA PKMVDFDMIL QVLSFFKGTI INGYRKKNAG VWPRVKVDTI 

       430        440        450        460        470        480 
YGKIIGQLHA DSAEISHDIM LREYKSLSAL EFEPCIEYDP VTNLSMFLKD KAIAHPNDNW 

       490        500        510        520        530        540 
LASFRRNLLS EDQKKHVKEA TSTNRLLIEF LESNDFDPYK EMEYLTTLEY LRDDDVAVSY 

       550        560        570        580        590        600 
SLKEKEVKVN GRIFAKLTKK LRNCQVMAEG ILADQIAPFF QGNGVIQDSI SLTKSTLAMS 

       610        620        630        640        650        660 
QLSFNSNKKR ITDCKERVSS NRNHDPKSKN RRRVATFITT DLQKYCLNWR YQTIKLFAHA 

       670        680        690        700        710        720 
INQLMGLPHF FEWIHLRLMD TTMFVGDPFN PPSDPTDCDL SRVPNDDIYI VSARGGIEGL 

       730        740        750        760        770        780 
CQKLWTMISI AAIQLAAARS HCRVACMVQG DNQVIAVTRE VRSDDSPEMV LTQLHQASDN 

       790        800        810        820        830        840 
FFKELIHVNH LIGHNLKDRE TIRSDTFFIY SKRIFKDGAI LSQVLKNSSK LVMVSGDLSE 

       850        860        870        880        890        900 
NTVMSCANIA STVARLCENG LPKDFCYYLN YIMSCVQTYF DSEFSYNNNS HPDLNQSWIE 

       910        920        930        940        950        960 
DISFVHSYVL TPAQLGGLSN LQYSRLYTRN IGDPGTTAFA EIKRLEAVGL LSPNIMTNIL 

       970        980        990       1000       1010       1020 
TRPPGNGDWA SLCNDPYSFN FETVASPNIV LKKHTQRVLF ETCSNPLLSG VHTEDNEAEE 

      1030       1040       1050       1060       1070       1080 
KALAEFLLNQ EVIHPRVAHA IMEASSVGRR KQIQGLVDTT NTVIKIALTR RPLGIKRLMR 

      1090       1100       1110       1120       1130       1140 
IVNYSSMHAM LFRDDVFSSN RSNHPLVSSN MCSLTLADYA RNRSWSPLTG GRKILGVSNP 

      1150       1160       1170       1180       1190       1200 
DTIELVEGEI LSVSGGCTRC DSGDEQFTWF HLPSNIELTD DTSKNPPMRV PYLGSKTQER 

      1210       1220       1230       1240       1250       1260 
RAASLAKIAH MSPHVKAALR ASSVLIWAYG DNEVNWTAAL TIAKSRCNIN LEYLRLLSPL 

      1270       1280       1290       1300       1310       1320 
PTAGNLQHRL DDGITQMTFT PASLYRCHLT FTYPMILKGY SLKKESKRGM WFINRVMLLG 

      1330       1340       1350       1360       1370       1380 
LSLIESIFPM TTTRTYDEIT LHLHSKFSCC IREAPVAVPF ELLGVAPELR TVTSNKFMYD 

      1390       1400       1410       1420       1430       1440 
PSPVSEGDFA RLDLAIFKSY ELNLESYPTI ELMNILSISS GKLIGQSVVS YDEDTSIKND 

      1450       1460       1470       1480       1490       1500 
AIIVYDNTRN WISEAQNSDV VRLFEYAALE VLLDCSYQLY YLRVRGLDNI VLYMGDLYKN 

      1510       1520       1530       1540       1550       1560 
MPGILLSNIA ATISHPVIHS RLHAVGLVNH NGSHQLADTD FIEMSAKLLV SCTRRVISGL 

      1570       1580       1590       1600       1610       1620 
YSGNKYDLLF PSVLDDNLNE KMLQLISRLC CLYTVLFATT REIPKIRGLS AEEKCSVLTE 

      1630       1640       1650       1660       1670       1680 
YLLSDAVKPL LSPDQVSSIM SPNIITFPAN LYYMSRKSLN LIREREDKDS ILALLFPQEP 

      1690       1700       1710       1720       1730       1740 
LLEFPSVQDI GARVKDPFTR QPAAFLQELD LSAPARYDAF TLSQIHPELT SPNPEEDYLV 

      1750       1760       1770       1780       1790       1800 
RYLFRGIGTA SSSWYKASHL LSVPEVRCAR HGNSLYLAEG SGAIMSLLEL HVPHETIYYN 

      1810       1820       1830       1840       1850       1860 
TLFSNEMNPP QRHFGPTPTQ FLNSVVYRNL QAEVTCKDGF VQEFRPLWRE NTEESDLTSD 

      1870       1880       1890       1900       1910       1920 
KAVGYITSAV PYRSVSLLHC DIEIPPGSNQ SLLDQLAINL SLIAMHSVRE GGVVIIKVLY 

      1930       1940       1950       1960       1970       1980 
AMGYYFHLLM NLFAPCSTKG YILSNGYACR GDMECYLVFV MGYLGGPTFV HEVVRMAKTL 

      1990       2000       2010       2020       2030       2040 
VQRHGTLLSK SDEITLTRLF TSQRQRVTDI LSSPLPRLIK YLRKNIDTAL IEAGGQPVRP 

      2050       2060       2070       2080       2090       2100 
FCAESLVSTL ADITQITQII ASHIDTVIRS VIYMEAEGDL ADTVFLFTPY NLSTDGKKRT 

      2110       2120       2130       2140       2150       2160 
SLKQCTRQIL EVTILGLRVE DLNKIGDVIS LVLKGMISME DLIPLRTYLK HSTCPKYLKA 

      2170       2180       2190       2200 
VLGITKLKEM FTDTSVLYLT RAQQKFYMKT IGNAVKGYYS NCDS

P11205 in FASTA format

View entry in original UniProtKB/Swiss-Prot format
View entry in raw text format (no links)
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	BLAST submission on ExPASy/SIB or at NCBI (USA)		Sequence analysis tools: ProtParam, ProtScale, Compute pI/Mw, PeptideMass, PeptideCutter, Dotlet (Java)
	ScanProsite, MotifScan		Submit a homology modeling request to SWISS-MODEL
	NPSA Sequence analysis tools