IL-8
C-X-C motif chemokine 8
Monocyte-derived neutrophil chemotactic factor
MDNCF
T-cell chemotactic factor
Neutrophil-activating protein 1
NAP-1
Protein 3-10C
Granulocyte chemotactic protein 1
GCP-1
Monocyte-derived neutrophil-activating peptide
MONAP
Emoctakin

Contains

MDNCF-a
     (IL8/NAP1 form I)
     (GCP/IL-8 protein IV)
Interleukin-8
     (IL-8(1-77))
     (MDNCF-b)
     (IL8/NAP1 form II)
     (GCP/IL-8 protein II)
     ((Ala-IL-8)77)
IL-8(5-77)
IL-8(6-77)
     (Lymphocyte-derived neutrophil-activating factor)
     (LYNAP)
     (Neutrophil-activating factor)
     (NAF)
     (MDNCF-c)
     (IL8/NAP1 form III)
     (GCP/IL-8 protein I)
     ((Ser-IL-8)72)
IL-8(7-77)
     (IL8/NAP1 form IV)
     (GCP/IL-8 protein V)
IL-8(8-77)
     (IL8/NAP1 form V)
     (GCP/IL-8 protein VI)
IL-8(9-77)
     (IL8/NAP1 form VI)
     (GCP/IL-8 protein III)

Gene name

	Name:	IL8
	Synonyms:	CXCL8

From

Homo sapiens (Human)

[TaxID: 9606]

Taxonomy

Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini; Catarrhini; Hominidae; Homo.

Protein existence

1: Evidence at protein level;

References

[1]	NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1). PubMed=2953813 [NCBI, ExPASy, EBI, Israel, Japan] Schmid J., Weissmann C.; "Induction of mRNA for a serine protease and a beta-thromboglobulin-like protein in mitogen-stimulated human leukocytes."; J. Immunol. 139:250-256(1987).
[2]	NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1). DOI=10.1084/jem.167.6.1883; PubMed=3260265 [NCBI, ExPASy, EBI, Israel, Japan] Matsushima K., Morishita K., Yoshimura T., Lavu S., Kobayashi Y., Lew W., Appella E., Kung H., Leonard E.J., Oppenheim J.J.; "Molecular cloning of a human monocyte-derived neutrophil chemotactic factor (MDNCF) and the induction of MDNCF mRNA by interleukin 1 and tumor necrosis factor."; J. Exp. Med. 167:1883-1893(1988).
[3]	NUCLEOTIDE SEQUENCE [GENOMIC DNA]. PubMed=2663993 [NCBI, ExPASy, EBI, Israel, Japan] Mukaida N., Shiroo M., Matsushima K.; "Genomic structure of the human monocyte-derived neutrophil chemotactic factor IL-8."; J. Immunol. 143:1366-1371(1989).
[4]	NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1). PubMed=2664463 [NCBI, ExPASy, EBI, Israel, Japan] Kowalski J., Denhardt D.T.; "Regulation of the mRNA for monocyte-derived neutrophil-activating peptide in differentiating HL60 promyelocytes."; Mol. Cell. Biol. 9:1946-1957(1989).
[5]	NUCLEOTIDE SEQUENCE [GENOMIC DNA]. TISSUE=Lung carcinoma; DOI=10.1016/0165-2478(90)90043-P; PubMed=2200751 [NCBI, ExPASy, EBI, Israel, Japan] Hotta K., Hayashi K., Ishikawa J., Tagawa M., Hashimoto K., Mizuno S., Suzuki K.; "Coding region structure of interleukin-8 gene of human lung giant cell carcinoma LU65C cells that produce LUCT/interleukin-8: homogeneity in interleukin-8 genes."; Immunol. Lett. 24:165-169(1990).
[6]	NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 2). TISSUE=Peripheral blood leukocyte; Jang J.S., Kim B.E.; Submitted (FEB-1998) to the EMBL/GenBank/DDBJ databases.
[7]	NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1). TISSUE=Neutrophil; DOI=10.1038/ng1285; PubMed=14702039 [NCBI, ExPASy, EBI, Israel, Japan] Ota T., Suzuki Y., Nishikawa T., Otsuki T., Sugiyama T., Irie R., Wakamatsu A., Hayashi K., Sato H., Nagai K., Kimura K., Makita H., Sekine M., Obayashi M., Nishi T., Shibahara T., Tanaka T., Ishii S., Yamamoto J., Saito K., Kawai Y., Isono Y., Nakamura Y., Nagahari K., Murakami K., Yasuda T., Iwayanagi T., Wagatsuma M., Shiratori A., Sudo H., Hosoiri T., Kaku Y., Kodaira H., Kondo H., Sugawara M., Takahashi M., Kanda K., Yokoi T., Furuya T., Kikkawa E., Omura Y., Abe K., Kamihara K., Katsuta N., Sato K., Tanikawa M., Yamazaki M., Ninomiya K., Ishibashi T., Yamashita H., Murakawa K., Fujimori K., Tanai H., Kimata M., Watanabe M., Hiraoka S., Chiba Y., Ishida S., Ono Y., Takiguchi S., Watanabe S., Yosida M., Hotuta T., Kusano J., Kanehori K., Takahashi-Fujii A., Hara H., Tanase T.-O., Nomura Y., Togiya S., Komai F., Hara R., Takeuchi K., Arita M., Imose N., Musashino K., Yuuki H., Oshima A., Sasaki N., Aotsuka S., Yoshikawa Y., Matsunawa H., Ichihara T., Shiohata N., Sano S., Moriya S., Momiyama H., Satoh N., Takami S., Terashima Y., Suzuki O., Nakagawa S., Senoh A., Mizoguchi H., Goto Y., Shimizu F., Wakebe H., Hishigaki H., Watanabe T., Sugiyama A., Takemoto M., Kawakami B., Yamazaki M., Watanabe K., Kumagai A., Itakura S., Fukuzumi Y., Fujimori Y., Komiyama M., Tashiro H., Tanigami A., Fujiwara T., Ono T., Yamada K., Fujii Y., Ozaki K., Hirao M., Ohmori Y., Kawabata A., Hikiji T., Kobatake N., Inagaki H., Ikema Y., Okamoto S., Okitani R., Kawakami T., Noguchi S., Itoh T., Shigeta K., Senba T., Matsumura K., Nakajima Y., Mizuno T., Morinaga M., Sasaki M., Togashi T., Oyama M., Hata H., Watanabe M., Komatsu T., Mizushima-Sugano J., Satoh T., Shirai Y., Takahashi Y., Nakagawa K., Okumura K., Nagase T., Nomura N., Kikuchi H., Masuho Y., Yamashita R., Nakai K., Yada T., Nakamura Y., Ohara O., Isogai T., Sugano S.; "Complete sequencing and characterization of 21,243 full-length human cDNAs."; Nat. Genet. 36:40-45(2004).
[8]	NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1). Kalnine N., Chen X., Rolfs A., Halleck A., Hines L., Eisenstein S., Koundinya M., Raphael J., Moreira D., Kelley T., LaBaer J., Lin Y., Phelan M., Farmer A.; "Cloning of human full-length CDSs in BD Creator(TM) system donor vector."; Submitted (MAY-2003) to the EMBL/GenBank/DDBJ databases.
[9]	NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1). Halleck A., Ebert L., Mkoundinya M., Schick M., Eisenstein S., Neubert P., Kstrang K., Schatten R., Shen B., Henze S., Mar W., Korn B., Zuo D., Hu Y., LaBaer J.; "Cloning of human full open reading frames in Gateway(TM) system entry vector (pDONR201)."; Submitted (JUN-2004) to the EMBL/GenBank/DDBJ databases.
[10]	NUCLEOTIDE SEQUENCE [GENOMIC DNA]. SeattleSNPs variation discovery resource; Submitted (JUN-2001) to the EMBL/GenBank/DDBJ databases.
[11]	NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Mural R.J., Istrail S., Sutton G.G., Florea L., Halpern A.L., Mobarry C.M., Lippert R., Walenz B., Shatkay H., Dew I., Miller J.R., Flanigan M.J., Edwards N.J., Bolanos R., Fasulo D., Halldorsson B.V., Hannenhalli S., Turner R., Yooseph S., Lu F., Nusskern D.R., Shue B.C., Zheng X.H., Zhong F., Delcher A.L., Huson D.H., Kravitz S.A., Mouchard L., Reinert K., Remington K.A., Clark A.G., Waterman M.S., Eichler E.E., Adams M.D., Hunkapiller M.W., Myers E.W., Venter J.C.; Submitted (JUL-2005) to the EMBL/GenBank/DDBJ databases.
[12]	NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1). TISSUE=Lung; DOI=10.1101/gr.2596504; PubMed=15489334 [NCBI, ExPASy, EBI, Israel, Japan] The MGC Project Team; "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."; Genome Res. 14:2121-2127(2004).
[13]	NUCLEOTIDE SEQUENCE [MRNA] OF 1-97 (ISOFORM 1). TISSUE=Kidney; King C.H., Gordon G.S., Konieczkowski M., Sedor J.R.; "cDNA cloning of human mesangial cell interleukin 8 by polymerase chain reaction."; Submitted (FEB-1992) to the EMBL/GenBank/DDBJ databases.
[14]	PROTEIN SEQUENCE OF 21-41, IDENTIFICATION OF MDNCF-A; IL-8(1-77); IL-8(6-77); IL-8(7-77); IL-8(8-77) AND IL-8(9-77), AND PROTEOLYTIC PROCESSING OF N-TERMINAL. DOI=10.1111/j.1432-1033.1989.tb14729.x; PubMed=2523801 [NCBI, ExPASy, EBI, Israel, Japan] van Damme J., van Beeumen J., Conings R., Decock B., Billiau A.; "Purification of granulocyte chemotactic peptide/interleukin-8 reveals N-terminal sequence heterogeneity similar to that of beta-thromboglobulin."; Eur. J. Biochem. 181:337-344(1989).
[15]	PROTEIN SEQUENCE OF 21-41, IDENTIFICATION OF MDNCF-A; IL-8(1-77); IL-8(6-77); IL-8(7-77); IL-8(8-77) AND IL-8(9-77), PROTEOLYTIC PROCESSING OF N-TERMINAL, AND FUNCTION. DOI=10.1002/eji.1830200933; PubMed=2145175 [NCBI, ExPASy, EBI, Israel, Japan] van Damme J., Rampart M., Coning R., Decock B., van Osselaer N., Willems J., Billiau A.; "The neutrophil-activating proteins interleukin 8 and beta-thromboglobulin: in vitro and in vivo comparison of NH2-terminally processed forms."; Eur. J. Immunol. 20:2113-2118(1990).
[16]	PROTEIN SEQUENCE OF 21-32, IDENTIFICATION OF MDNCF-A; IL-8(1-77) AND IL-8(6-77), AND PROTEOLYTIC PROCESSING OF N-TERMINAL. DOI=10.1016/0161-5890(89)90024-2; PubMed=2648135 [NCBI, ExPASy, EBI, Israel, Japan] Yoshimura T., Robinson E.A., Appella E., Matsushima K., Showalter S.D., Skeel A., Leonard E.J.; "Three forms of monocyte-derived neutrophil chemotactic factor (MDNCF) distinguished by different lengths of the amino-terminal sequence."; Mol. Immunol. 26:87-93(1989).
[17]	PROTEIN SEQUENCE OF 23-54, AND IDENTIFICATION OF IL-8(1-77). DOI=10.1084/jem.169.6.1895; PubMed=2659722 [NCBI, ExPASy, EBI, Israel, Japan] Suzuki K., Miyasaka H., Ota H., Yamakawa Y., Tagawa M., Kuramoto A., Mizuno S.; "Purification and partial primary sequence of a chemotactic protein for polymorphonuclear leukocytes derived from human lung giant cell carcinoma LU65C cells."; J. Exp. Med. 169:1895-1901(1989).
[18]	PROTEIN SEQUENCE OF 23-47. TISSUE=Skin; PubMed=1755384 [NCBI, ExPASy, EBI, Israel, Japan] Schroeder J.-M.; "Biochemical and biological characterization of NAP-1/IL-8-related cytokines in lesional psoriatic scale."; Adv. Exp. Med. Biol. 305:97-107(1991).
[19]	PROTEIN SEQUENCE OF 23-46, AND IDENTIFICATION OF IL-8(1-77). PubMed=2655583 [NCBI, ExPASy, EBI, Israel, Japan] Golds E.E., Mason P., Nyirkos P.; "Inflammatory cytokines induce synthesis and secretion of gro protein and a neutrophil chemotactic factor but not beta 2-microglobulin in human synovial cells and fibroblasts."; Biochem. J. 259:585-588(1989).
[20]	PROTEIN SEQUENCE OF 23-42. TISSUE=Chronic myeloid leukemia cell; DOI=10.1016/0165-2478(93)90071-9; PubMed=8344717 [NCBI, ExPASy, EBI, Israel, Japan] Suzuki K., Yamakawa Y., Matsuo Y., Kamiya T., Minowada J., Mizuno S.; "Isolation and amino acid sequence of a chemotactic protein, LECT/interleukin 8, from a human myeloid leukemia cell line, ML-1."; Immunol. Lett. 36:71-81(1993).
[21]	PROTEIN SEQUENCE OF 28-99, AND IDENTIFICATION OF IL-8(6-77). DOI=10.1016/S0006-291X(88)80364-4; PubMed=3279957 [NCBI, ExPASy, EBI, Israel, Japan] Gregory H., Young J., Schroeder J.-M., Mrowietz U., Christophers E.; "Structure determination of a human lymphocyte derived neutrophil activating peptide (LYNAP)."; Biochem. Biophys. Res. Commun. 151:883-890(1988).
[22]	PROTEIN SEQUENCE OF 28-69, AND IDENTIFICATION OF IL-8(6-77). DOI=10.1073/pnas.84.24.9233; PubMed=3480540 [NCBI, ExPASy, EBI, Israel, Japan] Yoshimura T., Matsushima K., Tanaka S., Robinson E.A., Appella E., Oppenheim J.J., Leonard E.J.; "Purification of a human monocyte-derived neutrophil chemotactic factor that has peptide sequence similarity to other host defense cytokines."; Proc. Natl. Acad. Sci. U.S.A. 84:9233-9237(1987).
[23]	PROTEIN SEQUENCE OF 28-59, AND IDENTIFICATION OF IL-8(6-77). DOI=10.1016/0006-291X(87)90432-3; PubMed=3322281 [NCBI, ExPASy, EBI, Israel, Japan] Walz A., Peveri P., Aschauer H., Baggiolini M.; "Purification and amino acid sequencing of NAF, a novel neutrophil-activating factor produced by monocytes."; Biochem. Biophys. Res. Commun. 149:755-761(1987).
[24]	PROTEOLYTIC PROCESSING OF N-TERMINAL BY THROMBIN, AND FUNCTION. PubMed=2212672 [NCBI, ExPASy, EBI, Israel, Japan] Hebert C.A., Luscinskas F.W., Kiely J.-M., Luis E.A., Darbonne W.C., Bennett G.L., Liu C.C., Obin M.S., Gimbrone M.A. Jr., Baker J.B.; "Endothelial and leukocyte forms of IL-8. Conversion by thrombin and interactions with neutrophils."; J. Immunol. 145:3033-3040(1990).
[25]	SYNTHESIS OF 28-99. DOI=10.1021/bi00226a021; PubMed=2007144 [NCBI, ExPASy, EBI, Israel, Japan] Clark-Lewis I., Mose B., Walz A., Baggiolini M., Scott G.J., Aebersold R.; "Chemical synthesis, purification, and characterization of two inflammatory proteins, neutrophil activating peptide 1 (interleukin-8) and neutrophil activating peptide."; Biochemistry 30:3128-3135(1991).
[26]	PROTEOLYTIC PROCESSING OF N-TERMINAL BY MMP9. PubMed=11023497 [NCBI, ExPASy, EBI, Israel, Japan] Van den Steen P.E., Proost P., Wuyts A., Van Damme J., Opdenakker G.; "Neutrophil gelatinase B potentiates interleukin-8 tenfold by aminoterminal processing, whereas it degrades CTAP-III, PF-4, and GRO-alpha and leaves RANTES and MCP-2 intact."; Blood 96:2673-2681(2000).
[27]	IDENTIFICATION OF IL-8(5-77) AND IL-8(6-77), FUNCTION, AND PROTEOLYTIC PROCESSING OF N-TERMINAL. DOI=10.1074/jbc.M112275200; PubMed=11978786 [NCBI, ExPASy, EBI, Israel, Japan] Schutyser E., Struyf S., Proost P., Opdenakker G., Laureys G., Verhasselt B., Peperstraete L., Van de Putte I., Saccani A., Allavena P., Mantovani A., Van Damme J.; "Identification of biologically active chemokine isoforms from ascitic fluid and elevated levels of CCL18/pulmonary and activation-regulated chemokine in ovarian carcinoma."; J. Biol. Chem. 277:24584-24593(2002).
[28]	REVIEW. DOI=10.1016/0014-5793(92)80909-Z; PubMed=1639201 [NCBI, ExPASy, EBI, Israel, Japan] Baggiolini M., Clark-Lewis I.; "Interleukin-8, a chemotactic and inflammatory cytokine."; FEBS Lett. 307:97-101(1992).
[29]	REVIEW. DOI=10.1016/S0065-2776(03)81001-5; PubMed=14711052 [NCBI, ExPASy, EBI, Israel, Japan] Struyf S., Proost P., Van Damme J.; "Regulation of the immune response by the interaction of chemokines and proteases."; Adv. Immunol. 81:1-44(2003).
[30]	STRUCTURE BY NMR OF 28-99. PubMed=2681204 [NCBI, ExPASy, EBI, Israel, Japan] Clore G.M., Appella E., Yamada M., Matsushima K., Gronenborn A.M.; "Determination of the secondary structure of interleukin-8 by nuclear magnetic resonance spectroscopy."; J. Biol. Chem. 264:18907-18911(1989).
[31]	STRUCTURE BY NMR. DOI=10.1021/bi00459a004; PubMed=2184886 [NCBI, ExPASy, EBI, Israel, Japan] Clore G.M., Appella E., Yamada M., Matsushima K., Gronenborn A.M.; "Three-dimensional structure of interleukin 8 in solution."; Biochemistry 29:1689-1696(1990).
[32]	STRUCTURE BY NMR OF 28-80. DOI=10.1111/j.1432-1033.1996.00026.x; PubMed=8631339 [NCBI, ExPASy, EBI, Israel, Japan] Sticht H., Auer M., Schmitt B., Besemer J., Horcher M., Kirsch T., Lindley I.J., Rosch P.; "Structure and activity of a chimeric interleukin-8-melanoma-growth-stimulatory-activity protein."; Eur. J. Biochem. 235:26-35(1996).
[33]	STRUCTURE BY NMR OF COMPLEX TO RECEPTOR. DOI=10.1016/S0969-2126(99)80022-7; PubMed=10368283 [NCBI, ExPASy, EBI, Israel, Japan] Skelton N.J., Quan C., Reilly D., Lowman H.; "Structure of a CXC chemokine-receptor fragment in complex with interleukin-8."; Structure 7:157-168(1999).
[34]	X-RAY CRYSTALLOGRAPHY (1.6 ANGSTROMS). PubMed=2182630 [NCBI, ExPASy, EBI, Israel, Japan] Baldwin E.T., Franklin K.A., Appella E., Yamada M., Matsushima K., Wlodawer A., Weber I.T.; "Crystallization of human interleukin-8. A protein chemotactic for neutrophils and T-lymphocytes."; J. Biol. Chem. 265:6851-6853(1990).
[35]	STRUCTURE BY NMR, AND COMPARISON WITH X-RAY STRUCTURE. DOI=10.1016/0022-2836(91)90518-B; PubMed=2005614 [NCBI, ExPASy, EBI, Israel, Japan] Clore G.M., Gronenborn A.M.; "Comparison of the solution nuclear magnetic resonance and crystal structures of interleukin-8. Possible implications for the mechanism of receptor binding."; J. Mol. Biol. 217:611-620(1991).
[36]	X-RAY CRYSTALLOGRAPHY (1.6 ANGSTROMS), AND STRUCTURE BY NMR. DOI=10.1073/pnas.88.2.502; PubMed=1988949 [NCBI, ExPASy, EBI, Israel, Japan] Baldwin E.T., Weber I.T., St Charles R., Xuan J.C., Appella E., Yamada M., Matsushima K., Edwards B.F., Clore G.M., Gronenborn A.M.; "Crystal structure of interleukin 8: symbiosis of NMR and crystallography."; Proc. Natl. Acad. Sci. U.S.A. 88:502-506(1991).
[37]	X-RAY CRYSTALLOGRAPHY (2.35 ANGSTROMS) OF MUTANT. DOI=10.1002/(SICI)1097-0134(20000301)38:4<361::AID-PROT2>3.3.CO;2-S; PubMed=10707023 [NCBI, ExPASy, EBI, Israel, Japan] Gerber N., Lowman H., Artis D.R., Eigenbrot C.; "Receptor-binding conformation of the 'ELR' motif of IL-8: X-ray structure of the L5C/H33C variant at 2.35 A resolution."; Proteins 38:361-367(2000).

Comments

FUNCTION: IL-8 is a chemotactic factor that attracts neutrophils, basophils, and T-cells, but not monocytes. It is also involved in neutrophil activation. It is released from several cell types in response to an inflammatory stimulus. IL-8(6-77) has a 5-10-fold higher activity on neutrophil activation, IL-8(5-77) has increased activity on neutrophil activation and IL-8(7-77) has a higher affinity to receptors CXCR1 and CXCR2 as compared to IL-8(1-77), respectively.
SUBUNIT: Homodimer.
SUBCELLULAR LOCATION: Secreted.
ALTERNATIVE PRODUCTS: 2 named isoforms [FASTA] produced by alternative splicing.

Name 1

Isoform ID P10145-1

This is the isoform sequence displayed in this entry.

Name 2

Isoform ID P10145-2

Features which should be applied to build the isoform sequence: VSP_001058.
PTM: Several N-terminal processed forms are produced by proteolytic cleavage after secretion from at least peripheral blood monocytes, leukcocytes and endothelial cells. In general, IL-8(1-77) is referred to as interleukin-8. IL-8(6-77) is the most promiment form.
SIMILARITY: Belongs to the intercrine alpha (chemokine CxC) family.
WEB RESOURCE: Name=Wikipedia; Note=Interleukin-8 entry; URL="http://en.wikipedia.org/wiki/Interleukin_8";.
WEB RESOURCE: Name=SeattleSNPs; URL="http://pga.gs.washington.edu/data/il8/";.

Copyright

Copyrighted by the UniProt Consortium, see http://www.uniprot.org/terms. Distributed under the Creative Commons Attribution-NoDerivs License.

Cross-references

Sequence databases

EMBL

M17017; AAA35611.1; -; mRNA.	[EMBL / GenBank / DDBJ] [CoDingSequence]
Y00787; CAA68742.1; -; mRNA.	[EMBL / GenBank / DDBJ] [CoDingSequence]
M28130; AAA59158.1; -; Genomic_DNA.	[EMBL / GenBank / DDBJ] [CoDingSequence]
M26383; AAA36323.1; -; mRNA.	[EMBL / GenBank / DDBJ] [CoDingSequence]
D14283; BAA03245.1; -; Genomic_DNA.	[EMBL / GenBank / DDBJ] [CoDingSequence]
AF043337; AAK00048.1; -; mRNA.	[EMBL / GenBank / DDBJ] [CoDingSequence]
AK311874; BAG34815.1; -; mRNA.	[EMBL / GenBank / DDBJ] [CoDingSequence]
BT007067; AAP35730.1; -; mRNA.	[EMBL / GenBank / DDBJ] [CoDingSequence]
CR542151; CAG46948.1; -; mRNA.	[EMBL / GenBank / DDBJ] [CoDingSequence]
AF385628; AAK60276.1; ALT_SEQ; Genomic_DNA.	[EMBL / GenBank / DDBJ] [CoDingSequence]
CH471057; EAX05688.1; -; Genomic_DNA.	[EMBL / GenBank / DDBJ] [CoDingSequence]
BC013615; AAH13615.1; -; mRNA.	[EMBL / GenBank / DDBJ] [CoDingSequence]
Z11686; CAA77745.1; -; mRNA.	[EMBL / GenBank / DDBJ] [CoDingSequence]

IPI

IPI00215621; -.
IPI00288914; -.

A37034; A37034.

NP_000575.1; -.

3D structure databases

PDB

1ICW; X-ray; 2.01 A; A/B=28-99.	[ExPASy / RCSB / EBI]
1IKL; NMR; -; A=28-99.	[ExPASy / RCSB / EBI]
1IKM; NMR; -; A=28-99.	[ExPASy / RCSB / EBI]
1IL8; NMR; -; A/B=28-99.	[ExPASy / RCSB / EBI]
1ILP; NMR; -; A/B=28-99.	[ExPASy / RCSB / EBI]
1ILQ; NMR; -; A/B=28-99.	[ExPASy / RCSB / EBI]
1QE6; X-ray; 2.35 A; A/B/C/D=28-99.	[ExPASy / RCSB / EBI]
1ROD; NMR; -; A/B=28-80.	[ExPASy / RCSB / EBI]
2IL8; NMR; -; A/B=28-99.	[ExPASy / RCSB / EBI]
3IL8; X-ray; 2.00 A; A=28-99.	[ExPASy / RCSB / EBI]

Detailed list of linked structures.

PDBsum

1ICW; -.
1IKL; -.
1IKM; -.
1IL8; -.
1ILP; -.
1ILQ; -.
1QE6; -.
1ROD; -.
2IL8; -.
3IL8; -.

ModBase

P10145.

Protein-protein interaction databases

DIP

DIP:3778N; -.

Enzyme and pathway databases

Pathway_Interaction_DB

nfat_tfpathway; Calcineurin-regulated NFAT-dependent transcription in lymphocytes.
lysophospholipid_pathway; LPA receptor mediated events.
syndecan_2_pathway; Syndecan-2-mediated signaling events.
syndecan_3_pathway; Syndecan-3-mediated signaling events.

Reactome

REACT_14797; Signaling by GPCR.

Organism-specific databases

GC04P074845; -.

HIX0004281; -.

HGNC:6025; IL8.

IL8.

146930; gene. [NCBI / EBI]

PharmGKB

PA29841; -.

Gene expression databases

P10145; -.

P10145; -.

HS_IL8; -.

ENSG00000169429; Homo sapiens.

Ontologies

GO:0005615;	Cellular component: extracellular space (traceable author statement from ProtInc).
GO:0008009;	Molecular function: chemokine activity (traceable author statement from ProtInc).
GO:0005153;	Molecular function: interleukin-8 receptor binding (inferred from physical interaction from UniProtKB).
GO:0001525;	Biological process: angiogenesis (traceable author statement from UniProtKB).
GO:0019722;	Biological process: calcium-mediated signaling (traceable author statement from UniProtKB).
GO:0007050;	Biological process: cell cycle arrest (inferred from direct assay from UniProtKB).
GO:0007186;	Biological process: G-protein coupled receptor protein signaling pathway (traceable author statement from ProtInc).
GO:0006955;	Biological process: immune response (inferred from electronic annotation from InterPro).
GO:0050930;	Biological process: induction of positive chemotaxis (inferred from genetic interaction from UniProtKB).
GO:0006954;	Biological process: inflammatory response (traceable author statement from UniProtKB).
GO:0008285;	Biological process: negative regulation of cell proliferation (traceable author statement from ProtInc).
GO:0042119;	Biological process: neutrophil activation (traceable author statement from UniProtKB).
GO:0030593;	Biological process: neutrophil chemotaxis (inferred from genetic interaction from UniProtKB).
GO:0030155;	Biological process: regulation of cell adhesion (inferred from direct assay from UniProtKB).
GO:0045091;	Biological process: regulation of retroviral genome replication (inferred from direct assay from UniProtKB).
QuickGo view.

Family and domain databases

InterPro

IPR002473; C-X-C/Interlkn_8.
IPR001811; Chemokine_IL8.
IPR001089; CXC_chmkine_smll.
IPR018048; Small_chemokine_CXC_CS.
Graphical view of domain structure.

Pfam

PF00048; IL8; 1.
Pfam graphical view of domain structure.

PRINTS

PR00436; INTERLEUKIN8.
PR00437; SMALLCYTKCXC.

SMART

SM00199; SCY; 1.
SMART graphical view of domain structure.

PROSITE

PS00471; SMALL_CYTOKINES_CXC; 1.

Other

SWISS-3DIMAGE

P10145.

Genome annotation databases

Ensembl

ENSG00000169429; Homo sapiens. [Contig view]

GeneID

3576; -.

KEGG

hsa:3576; -.

Phylogenomic databases

HOGENOM

P10145; -.

HOVERGEN

P10145; -.

Other

DrugBank

DB01009; Ketoprofen.
DB01001; Salbutamol.
DB00641; Simvastatin.
DB00744; Zileuton.

13978; -.

P10145; -.

IL8; Homo sapiens.

View cluster of proteins with at least 50% / 90% / 100% identity.

Keywords

3D-structure; Alternative splicing; Chemotaxis; Cytokine; Direct protein sequencing; Disulfide bond; Inflammatory response; Secreted; Signal.

Features

Feature table viewer

Feature aligner

`Key`	`From To`	`Length`	`Description`	`FTId`
`SIGNAL`	`1 20`	`20`
`CHAIN`	`21 99`	`79`	`MDNCF-a.`	`PRO_0000005126`
`CHAIN`	`23 99`	`77`	`Interleukin-8.`	`PRO_0000005127`
`CHAIN`	`27 99`	`73`	`IL-8(5-77).`	`PRO_0000041948`
`CHAIN`	`28 99`	`72`	`IL-8(6-77).`	`PRO_0000005128`
`CHAIN`	`29 99`	`71`	`IL-8(7-77).`	`PRO_0000005129`
`CHAIN`	`30 99`	`70`	`IL-8(8-77).`	`PRO_0000005130`
`CHAIN`	`31 99`	`69`	`IL-8(9-77).`	`PRO_0000005131`
`SITE`	`27 28`	`2`	`Cleavage; by thrombin (Probable).`
`SITE`	`28 29`	`2`	`Cleavage; by MMP9.`
`DISULFID`	`34 61`
`DISULFID`	`36 77`
`VAR_SEQ`	`92 99`		`FLKRAENS -> AEVPENRGMDS (in isoform 2).`	`VSP_001058`
`CONFLICT`	`53 53`		`R -> L (in Ref. 17; AA sequence).`
`HELIX`	`46 48`	`3`
`STRAND`	`49 55`	`7`
`STRAND`	`58 60`	`3`
`STRAND`	`65 70`	`6`
`STRAND`	`75 78`	`4`
`HELIX`	`83 96`	`14`

Sequence information

Length: 99 AA [This is the length of the unprocessed precursor]

Molecular weight: 11098 Da [This is the MW of the unprocessed precursor]

CRC64: 15C649996E89319F [This is a checksum on the sequence]

        10         20         30         40         50         60 
MTSKLAVALL AAFLISAALC EGAVLPRSAK ELRCQCIKTY SKPFHPKFIK ELRVIESGPH 

        70         80         90 
CANTEIIVKL SDGRELCLDP KENWVQRVVE KFLKRAENS

P10145 in FASTA format

View entry in raw text format (no links)
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	BLAST submission on ExPASy/SIB or at NCBI (USA)		Sequence analysis tools: ProtParam, ProtScale, Compute pI/Mw, PeptideMass, PeptideCutter, Dotlet (Java)
	ScanProsite, MotifScan		Submit a homology modeling request to SWISS-MODEL
	NPSA Sequence analysis tools