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[1]
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NUCLEOTIDE SEQUENCE [GENOMIC DNA].
STRAIN=ATCC 17707 / LMG 1207 / Stanier 345 / H-20-R;
PubMed=2820933 [NCBI, ExPASy, EBI, Israel, Japan]
Andersen K.,
Caton J.;
"Sequence analysis of the Alcaligenes eutrophus chromosomally encoded ribulose bisphosphate carboxylase large and small subunit genes and their gene products.";
J. Bacteriol. 169:4547-4558(1987).
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[2]
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SEQUENCE REVISION.
Andersen K.;
Submitted (AUG-1998) to the EMBL/GenBank/DDBJ databases.
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[3]
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X-RAY CRYSTALLOGRAPHY (2.7 ANGSTROMS).
STRAIN=ATCC 17707 / LMG 1207 / Stanier 345 / H-20-R;
DOI=10.1006/jmbi.1999.2701; PubMed=10329167 [NCBI, ExPASy, EBI, Israel, Japan]
Hansen S.,
Vollan V.B.,
Hough E.,
Andersen K.;
"The crystal structure of rubisco from Alcaligenes eutrophus reveals a novel central eight-stranded beta-barrel formed by beta-strands from four subunits.";
J. Mol. Biol. 288:609-621(1999).
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- FUNCTION: RuBisCO catalyzes two reactions: the carboxylation of D-ribulose 1,5-bisphosphate, the primary event in carbon dioxide fixation, as well as the oxidative fragmentation of the pentose substrate. Both reactions occur simultaneously and in competition at the same active site.
- CATALYTIC ACTIVITY: 2 3-phospho-D-glycerate + 2 H+ = D-ribulose 1,5-bisphosphate + CO2 + H2O.
- CATALYTIC ACTIVITY: 3-phospho-D-glycerate + 2-phosphoglycolate = D-ribulose 1,5-bisphosphate + O2.
- COFACTOR: Binds 1 magnesium ion per subunit.
- SUBUNIT: Heterohexadecamer of 8 large chains and 8 small chains; disulfide-linked. The disulfide link is formed within the large subunit homodimers.
- PTM: The disulfide bond which can form between Cys-278 in the large chain dimeric partners within the hexadecamer appears to be associated with oxidative stress and protein turnover (By similarity).
- MISCELLANEOUS: The basic functional RuBisCO is composed of a large chain homodimer in a "head-to-tail" conformation. In form I RuBisCO this homodimer is arranged in a barrel-like tetramer with the small subunits forming a tetrameric "cap" on each end of the "barrel".
- SIMILARITY: Belongs to the RuBisCO large chain family. Type I subfamily.
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Copyrighted by the UniProt Consortium, see http://www.uniprot.org/terms.
Distributed under the Creative Commons Attribution-NoDerivs License.
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| Length: 486 AA [This is the length of the unprocessed precursor] |
Molecular weight: 53840 Da [This is the MW of the unprocessed precursor] |
CRC64: 66EAB4A11982FAB5 [This is a checksum on the sequence] |
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10 20 30 40 50 60
MNAPETIQAK PRKRYDAGVM KYKEMGYWDG DYVPKDTDVL ALFRITPQDG VDPVEAAAAV
70 80 90 100 110 120
AGESSTATWT VVWTDRLTAC DMYRAKAYRV DPVPNNPEQF FCYVAYDLSL FEEGSIANLT
130 140 150 160 170 180
ASIIGNVFSF KPIKAARLED MRFPVAYVKT FAGPSTGIIV ERERLDKFGR PLLGATTKPK
190 200 210 220 230 240
LGLSGRNYGR VVYEGLKGGL DFMKDDENIN SQPFMHWRDR FLFVMDAVNK ASAATGEVKG
250 260 270 280 290 300
SYLNVTAGTM EEMYRRAEFA KSLGSVIIMV DLIVGWTCIQ SMSNWCRQND MILHLHRAGH
310 320 330 340 350 360
GTYTRQKNHG VSFRVIAKWL RLAGVDHMHT GTAVGKLEGD PLTVQGYYNV CRDAYTQTDL
370 380 390 400 410 420
TRGLFFDQDW ASLRKVMPVA SGGIHAGQMH QLIHLFGDDV VLQFGGGTIG HPQGIQAGAT
430 440 450 460 470 480
ANRVALEAMV LARNEGRDIL NEGPEILRDA ARWCAPLRAA LDTWGDITFN YTPTDTSDFV
PTASVA
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P0C2C2 in FASTA format |
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ExPASy Home page
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