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UniProtKB/Swiss-Prot entry P0AFH0

[Entry info] [Name and origin] [References] [Comments] [Cross-references] [Keywords] [Features] [Sequence] [Tools]

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Entry information
Entry name OGT_ECOLI
Primary accession number P0AFH0
Secondary accession numbers P09168 P78284
Integrated into Swiss-Prot on December 20, 2005
Sequence was last modified on December 20, 2005 (Sequence version 1)
Annotations were last modified on    December 16, 2008 (Entry version 32)
Name and origin of the protein
Protein name Methylated-DNA--protein-cysteine methyltransferase
Synonyms EC 2.1.1.63
6-O-methylguanine-DNA methyltransferase
MGMT
O-6-methylguanine-DNA-alkyltransferase
Gene name
Name: ogt
OrderedLocusNames: b1335, JW1329
From
Escherichia coli (strain K12) [TaxID: 83333] [HAMAP proteome]
Taxonomy Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacteriales; Enterobacteriaceae; Escherichia.
Protein existence 1: Evidence at protein level;
References
[1]
 NUCLEOTIDE SEQUENCE [GENOMIC DNA], AND PARTIAL PROTEIN SEQUENCE.
DOI=10.1093/nar/15.22.9177; PubMed=2825131 [NCBI, ExPASy, EBI, Israel, Japan]
Potter P.M., Wilkinson M.C., Fitton J., Carr F.J., Brennand J., Cooper D.P., Margison G.P.;
"Characterisation and nucleotide sequence of ogt, the O6-alkylguanine-DNA-alkyltransferase gene of E. coli.";
Nucleic Acids Res. 15:9177-9193(1987).
[2]
 NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
STRAIN=K12 / W3110 / ATCC 27325 / DSM 5911;
DOI=10.1093/dnares/3.6.363; PubMed=9097039 [NCBI, ExPASy, EBI, Israel, Japan]
Aiba H., Baba T., Fujita K., Hayashi K., Inada T., Isono K., Itoh T., Kasai H., Kashimoto K., Kimura S., Kitakawa M., Kitagawa M., Makino K., Miki T., Mizobuchi K., Mori H., Mori T., Motomura K., Nakade S., Nakamura Y., Nashimoto H., Nishio Y., Oshima T., Saito N., Sampei G., Seki Y., Sivasundaram S., Tagami H., Takeda J., Takemoto K., Takeuchi Y., Wada C., Yamamoto Y., Horiuchi T.;
"A 570-kb DNA sequence of the Escherichia coli K-12 genome corresponding to the 28.0-40.1 min region on the linkage map.";
DNA Res. 3:363-377(1996).
[3]
 NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
STRAIN=K12 / MG1655 / ATCC 47076;
DOI=10.1126/science.277.5331.1453; PubMed=9278503 [NCBI, ExPASy, EBI, Israel, Japan]
Blattner F.R., Plunkett G. III, Bloch C.A., Perna N.T., Burland V., Riley M., Collado-Vides J., Glasner J.D., Rode C.K., Mayhew G.F., Gregor J., Davis N.W., Kirkpatrick H.A., Goeden M.A., Rose D.J., Mau B., Shao Y.;
"The complete genome sequence of Escherichia coli K-12.";
Science 277:1453-1474(1997).
[4]
 NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
STRAIN=K12 / W3110 / ATCC 27325 / DSM 5911;
DOI=10.1038/msb4100049; PubMed=16738553 [NCBI, ExPASy, EBI, Israel, Japan]
Hayashi K., Morooka N., Yamamoto Y., Fujita K., Isono K., Choi S., Ohtsubo E., Baba T., Wanner B.L., Mori H., Horiuchi T.;
"Highly accurate genome sequences of Escherichia coli K-12 strains MG1655 and W3110.";
Mol. Syst. Biol. 2:E1-E5(2006).
[5]
 MUTAGENESIS OF CYSTEINE RESIDUES.
DOI=10.1016/S0006-291X(05)81510-4; PubMed=1520330 [NCBI, ExPASy, EBI, Israel, Japan]
Harris L.C., Potter P.M., Margison G.P.;
"Site directed mutagenesis of two cysteine residues in the E. coli ogt O6-alkylguanine DNA alkyltransferase protein.";
Biochem. Biophys. Res. Commun. 187:425-431(1992).
Comments
  • FUNCTION: Involved in the cellular defense against the biological effects of O6-methylguanine (O6-MeG) in DNA. Repairs alkylated guanine in DNA by stoichiometrically transferring the alkyl group at the O-6 position to a cysteine residue in the enzyme. This is a suicide reaction: the enzyme is irreversibly inactivated. Can also repair O-4-methylthymine.
  • CATALYTIC ACTIVITY: DNA (containing 6-O-methylguanine) + protein L-cysteine = DNA (without 6-O-methylguanine) + protein S-methyl-L-cysteine.
  • SIMILARITY: Belongs to the MGMT family.
Copyright
Copyrighted by the UniProt Consortium, see http://www.uniprot.org/terms. Distributed under the Creative Commons Attribution-NoDerivs License.
Cross-references
Sequence databases
EMBL
Y00495; CAA68548.1; -; Genomic_DNA.[EMBL / GenBank / DDBJ] [CoDingSequence]
U00096; AAC74417.1; -; Genomic_DNA.[EMBL / GenBank / DDBJ] [CoDingSequence]
AP009048; BAA14928.1; -; Genomic_DNA.[EMBL / GenBank / DDBJ] [CoDingSequence]
PIR B64883; XUECAD.
RefSeq AP_001963.1; -.
NP_415851.1; -.
3D structure databases
HSSP P16455; 1QNT. [HSSP ENTRY / PDB]
ModBase P0AFH0.
Enzyme and pathway databases
BioCyc EcoCyc:EG10668-MON; -.
Organism-specific databases
EchoBASE EB0662; -.
EcoGene EG10668; ogt.
Ontologies
GO
GO:0003908; Molecular function: methylated-DNA-[protein]-cysteine S-methyltransferase activity (inferred from electronic annotation from InterPro).
GO:0006281; Biological process: DNA repair (inferred from electronic annotation from InterPro).
QuickGo view.
Family and domain databases
InterPro IPR001497; MethylDNA_cys_MeTrfase_AS.
IPR014048; MethylDNA_cys_MeTrfase_DNA_bd.
IPR008332; MethylG_MeTrfase.
IPR011991; Wing_hlx_DNA_bd.
Graphical view of domain structure.
Gene3D G3DSA:1.10.10.10; Wing_hlx_DNA_bd; 1.
PANTHER PTHR10815; MethylDNA_cys_mtrans_DNA_bd; 1.
Pfam PF01035; DNA_binding_1; 1.
PF02870; Methyltransf_1N; 1.
Pfam graphical view of domain structure.
TIGRFAMs TIGR00589; ogt; 1.
PROSITE PS00374; MGMT; 1.
Genome annotation databases
GeneID 945853; -.
GenomeReviews AP009048_GR; JW1329.
U00096_GR; b1335.
KEGG ecj:JW1329; -.
eco:b1335; -.
Phylogenomic databases
HOGENOM P0AFH0; -.
Genome annotation databases
CMR P0AFH0; b1335.
Other
ProtoNet P0AFH0.
UniRef View cluster of proteins with at least 50% / 90% / 100% identity.
Keywords
Complete proteome; Direct protein sequencing; DNA damage; DNA repair; Methyltransferase; Transferase.
Features
SEVIEWER logo Feature table viewer
KeyFrom   To Length Description FTId
CHAIN   1   171  171     Methylated-DNA--protein-cysteine methyltransferase. PRO_0000139365
ACT_SITE   139   139        Nucleophile; methyl group acceptor (Probable). 
MUTAGEN   102   102        C->G: Little effect on the activity. 
MUTAGEN   139   139        C->G: Eliminates the activity. 
CONFLICT   37    37        R -> A (in Ref. 1; CAA68548). 
CONFLICT   68    68        E -> D (in Ref. 1; CAA68548). 
Sequence information
Length: 171 AA [This is the length of the unprocessed precursor] Molecular weight: 19179 Da [This is the MW of the unprocessed precursor] CRC64: 39EFBF1539F67D26 [This is a checksum on the sequence] 
        10         20         30         40         50         60 
MLRLLEEKIA TPLGPLWVIC DEQFRLRAVE WEEYSERMVQ LLDIHYRKEG YERISATNPG 

        70         80         90        100        110        120 
GLSDKLREYF AGNLSIIDTL PTATGGTPFQ REVWKTLRTI PCGQVMHYGQ LAEQLGRPGA 

       130        140        150        160        170 
ARAVGAANGS NPISIVVPCH RVIGRNGTMT GYAGGVQRKE WLLRHEGYLL L
P0AFH0 in FASTA format

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