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UniProtKB/Swiss-Prot entry P0AEC8


[Entry info] [Name and origin] [References] [Comments] [Cross-references] [Keywords] [Features] [Sequence] [Tools]

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Entry information
Entry name DCUS_ECOLI
Primary accession number P0AEC8
Secondary accession numbers P39272 P76795 Q2M6H9
Integrated into Swiss-Prot on December 6, 2005
Sequence was last modified on December 6, 2005 (Sequence version 1)
Annotations were last modified on    July 22, 2008 (Entry version 36)
Name and origin of the protein
Protein name Sensor protein dcuS
Synonym EC 2.7.13.3
Gene name
Name: dcuS
Synonyms: yjdH
OrderedLocusNames: b4125, JW4086
From
Escherichia coli (strain K12) [TaxID: 83333] [HAMAP proteome]
Taxonomy Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacteriales; Enterobacteriaceae; Escherichia.
Protein existence 1: Evidence at protein level;
References
[1]
NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
STRAIN=K12 / MG1655 / ATCC 47076;
DOI=10.1093/nar/23.12.2105; PubMed=7610040 [NCBI, ExPASy, EBI, Israel, Japan]
Burland V.D., Plunkett G. III, Sofia H.J., Daniels D.L., Blattner F.R.;
"Analysis of the Escherichia coli genome VI: DNA sequence of the region from 92.8 through 100 minutes.";
Nucleic Acids Res. 23:2105-2119(1995).
[2]
NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
STRAIN=K12 / MG1655 / ATCC 47076;
DOI=10.1126/science.277.5331.1453; PubMed=9278503 [NCBI, ExPASy, EBI, Israel, Japan]
Blattner F.R., Plunkett G. III, Bloch C.A., Perna N.T., Burland V., Riley M., Collado-Vides J., Glasner J.D., Rode C.K., Mayhew G.F., Gregor J., Davis N.W., Kirkpatrick H.A., Goeden M.A., Rose D.J., Mau B., Shao Y.;
"The complete genome sequence of Escherichia coli K-12.";
Science 277:1453-1474(1997).
[3]
NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
STRAIN=K12 / W3110 / ATCC 27325 / DSM 5911;
DOI=10.1038/msb4100049; PubMed=16738553 [NCBI, ExPASy, EBI, Israel, Japan]
Hayashi K., Morooka N., Yamamoto Y., Fujita K., Isono K., Choi S., Ohtsubo E., Baba T., Wanner B.L., Mori H., Horiuchi T.;
"Highly accurate genome sequences of Escherichia coli K-12 strains MG1655 and W3110.";
Mol. Syst. Biol. 2:E1-E5(2006).
[4]
CHARACTERIZATION.
STRAIN=K12 / MC4100 / ATCC 35695 / DSM 6574;
PubMed=9765574 [NCBI, ExPASy, EBI, Israel, Japan]
Zientz E., Bongaerts J., Unden G.;
"Fumarate regulation of gene expression in Escherichia coli by the DcuSR (dcuSR genes) two-component regulatory system.";
J. Bacteriol. 180:5421-5425(1998).
[5]
CHARACTERIZATION, AND TOPOLOGY.
STRAIN=K12 / MC4100 / ATCC 35695 / DSM 6574;
PubMed=9973351 [NCBI, ExPASy, EBI, Israel, Japan]
Golby P., Davies S., Kelly D.J., Guest J.R., Andrews S.C.;
"Identification and characterization of a two-component sensor-kinase and response-regulator system (DcuS-DcuR) controlling gene expression in response to C4-dicarboxylates in Escherichia coli.";
J. Bacteriol. 181:1238-1248(1999).
[6]
CHARACTERIZATION.
STRAIN=K12 / AN387;
DOI=10.1074/jbc.M204482200; PubMed=12167640 [NCBI, ExPASy, EBI, Israel, Japan]
Janausch I.G., Garcia-Moreno I., Unden G.;
"Function of DcuS from Escherichia coli as a fumarate-stimulated histidine protein kinase in vitro.";
J. Biol. Chem. 277:39809-39814(2002).
[7]
TOPOLOGY [LARGE SCALE ANALYSIS].
STRAIN=K12 / MG1655 / ATCC 47076;
DOI=10.1126/science.1109730; PubMed=15919996 [NCBI, ExPASy, EBI, Israel, Japan]
Daley D.O., Rapp M., Granseth E., Melen K., Drew D., von Heijne G.;
"Global topology analysis of the Escherichia coli inner membrane proteome.";
Science 308:1321-1323(2005).
Comments
  • FUNCTION: Member of the two-component regulatory system dcuR/dcuS. Involved in the C4-dicarboxylate-stimulated regulation of the genes encoding the anaerobic fumarate respiratory system (frdABCD; nuoAN; dcuB; dcuC; sdhCDAB; etc.). Weakly regulates the aerobic C4-dicarboxylate transporter dctA. Activates dcuR by phosphorylation.
  • CATALYTIC ACTIVITY: ATP + protein L-histidine = ADP + protein N-phospho-L-histidine.
  • ENZYME REGULATION: Autophosphorylation is stimulated by the presence of fumarate or succinate.
  • INTERACTION:
    P0AC41:sdhA; NbExp=1; IntAct=EBI-1134683, EBI-371263;
  • SUBCELLULAR LOCATION: Cell inner membrane; Multi-pass membrane protein.
  • PTM: Autophosphorylated. The phosphoryl group is rapidely transferred to dcuR.
  • MISCELLANEOUS: The region encompassing approximately residues 42 to 181 has been shown to be periplasmic, however exactly which residues are periplasmic is not clear.
  • SIMILARITY: Contains 1 histidine kinase domain.
  • SIMILARITY: Contains 1 PAS (PER-ARNT-SIM) domain.
Copyright
Copyrighted by the UniProt Consortium, see http://www.uniprot.org/terms. Distributed under the Creative Commons Attribution-NoDerivs License.
Cross-references
Sequence databases
EMBL
U14003; AAA97025.1; -; Genomic_DNA.[EMBL / GenBank / DDBJ] [CoDingSequence]
U00096; AAC77086.1; -; Genomic_DNA.[EMBL / GenBank / DDBJ] [CoDingSequence]
AP009048; BAE78127.1; -; Genomic_DNA.[EMBL / GenBank / DDBJ] [CoDingSequence]
PIR D65222; D65222.
RefSeq AP_004626.1; -.
NP_418549.1; -.
3D structure databases
PDB
1OJG; NMR; -; A=45-180.[ExPASy / RCSB / EBI]
PDBsum 1OJG; -.
ModBase P0AEC8.
Protein-protein interaction databases
IntAct P0AEC8; -.
Enzyme and pathway databases
BioCyc EcoCyc:DCUS-MON; -.
Organism-specific databases
EchoBASE EB2358; -.
EcoGene EG12465; dcuS.
Ontologies
GO
GO:0005515; Molecular function: protein binding (inferred from physical interaction from IntAct).
QuickGo view.
Family and domain databases
InterPro IPR003594; ATP_bd_ATPase.
IPR000014; PAS.
IPR013767; PAS_fold.
IPR004358; Sig_transdc_His_kin-like_C.
IPR005467; Sig_transdc_His_kinase_core.
Graphical view of domain structure.
Gene3D G3DSA:3.30.565.10; ATP_bd_ATPase; 1.
Pfam PF02518; HATPase_c; 1.
PF00989; PAS; 1.
Pfam graphical view of domain structure.
PRINTS PR00344; BCTRLSENSOR.
SMART SM00387; HATPase_c; 1.
SM00091; PAS; 1.
SMART graphical view of domain structure.
TIGRFAMs TIGR00229; sensory_box; 1.
PROSITE PS50109; HIS_KIN; 1.
PS50112; PAS; 1.
PROSITE graphical view of domain structure (profiles).
BLOCKS P0AEC8.
Genome annotation databases
GeneID 948639; -.
GenomeReviews U00096_GR; b4125.
AP009048_GR; JW4086.
KEGG ecj:JW4086; -.
eco:b4125; -.
Phylogenomic databases
HOGENOM P0AEC8; -.
Genome annotation databases
CMR P0AEC8; b4125.
Other
ProtoNet P0AEC8.
UniRef View cluster of proteins with at least 50% / 90% / 100% identity.
Keywords
3D-structure; Cell inner membrane; Cell membrane; Complete proteome; Kinase; Membrane; Phosphoprotein; Transferase; Transmembrane; Two-component regulatory system.
Features
SEVIEWER logo Feature table viewer FT aligner logo Feature aligner
KeyFrom   To Length Description FTId
CHAIN   1   543  543     Sensor protein dcuS. PRO_0000074750
TOPO_DOM   1    20  20     Cytoplasmic (Potential). 
TRANSMEM   21    41  21     Potential. 
TOPO_DOM   42   181  140     Periplasmic (Potential). 
TRANSMEM   182   202  21     Potential. 
TOPO_DOM   203   543  341     Cytoplasmic (Potential). 
DOMAIN   220   291  72     PAS. 
DOMAIN   346   538  193     Histidine kinase. 
MOD_RES   349   349        Phosphohistidine; by autocatalysis (By similarity). 
HELIX   46    63  18      
HELIX   68    73  6      
HELIX   77    79  3      
TURN   82    84  3      
HELIX   85    93  9      
STRAND   97   104  8      
STRAND   111   113  3      
TURN   114   117  4      
HELIX   125   128  4      
STRAND   134   138  5      
TURN   141   143  3      
STRAND   145   153  9      
STRAND   155   157  3      
STRAND   159   167  9      
HELIX   173   178  6      
Sequence information
Length: 543 AA [This is the length of the unprocessed precursor] Molecular weight: 60551 Da [This is the MW of the unprocessed precursor] CRC64: 653C369344CBA238 [This is a checksum on the sequence]
        10         20         30         40         50         60 
MRHSLPYRML RKRPMKLSTT VILMVSAVLF SVLLVVHLIY FSQISDMTRD GLANKALAVA 

        70         80         90        100        110        120 
RTLADSPEIR QGLQKKPQES GIQAIAEAVR KRNDLLFIVV TDMQSLRYSH PEAQRIGQPF 

       130        140        150        160        170        180 
KGDDILKALN GEENVAINRG FLAQALRVFT PIYDENHKQI GVVAIGLELS RVTQQINDSR 

       190        200        210        220        230        240 
WSIIWSVLFG MLVGLIGTCI LVKVLKKILF GLEPYEISTL FEQRQAMLQS IKEGVVAVDD 

       250        260        270        280        290        300 
RGEVTLINDA AQELLNYRKS QDDEKLSTLS HSWSQVVDVS EVLRDGTPRR DEEITIKDRL 

       310        320        330        340        350        360 
LLINTVPVRS NGVIIGAIST FRDKTEVRKL MQRLDGLVNY ADALRERSHE FMNKLHVILG 

       370        380        390        400        410        420 
LLHLKSYKQL EDYILKTANN YQEEIGSLLG KIKSPVIAGF LISKINRATD LGHTLILNSE 

       430        440        450        460        470        480 
SQLPDSGSED QVATLITTLG NLIENALEAL GPEPGGEISV TLHYRHGWLH CEVNDDGPGI 

       490        500        510        520        530        540 
APDKIDHIFD KGVSTKGSER GVGLALVKQQ VENLGGSIAV ESEPGIFTQF FVQIPWDGER 


SNR 

P0AEC8 in FASTA format

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