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UniProtKB/Swiss-Prot entry P0A9A9


[Entry info] [Name and origin] [References] [Comments] [Cross-references] [Keywords] [Features] [Sequence] [Tools]

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Entry information
Entry name FUR_ECOLI
Primary accession number P0A9A9
Secondary accession number P06975
Integrated into Swiss-Prot on April 1, 1988
Sequence was last modified on April 1, 1988 (Sequence version 1)
Annotations were last modified on    September 2, 2008 (Entry version 38)
Name and origin of the protein
Protein name Ferric uptake regulation protein
Synonym Ferric uptake regulator
Gene name
Name: fur
OrderedLocusNames: b0683, JW0669
From
Escherichia coli (strain K12) [TaxID: 83333] [HAMAP proteome]
Taxonomy Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacteriales; Enterobacteriaceae; Escherichia.
Protein existence 1: Evidence at protein level;
References
[1]
NUCLEOTIDE SEQUENCE [GENOMIC DNA].
DOI=10.1007/BF00383321; PubMed=2993806 [NCBI, ExPASy, EBI, Israel, Japan]
Schaeffer S., Hantke K., Braun V.;
"Nucleotide sequence of the iron regulatory gene fur.";
Mol. Gen. Genet. 200:110-113(1985).
[2]
NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
STRAIN=K12 / W3110 / ATCC 27325 / DSM 5911;
DOI=10.1093/dnares/3.3.137; PubMed=8905232 [NCBI, ExPASy, EBI, Israel, Japan]
Oshima T., Aiba H., Baba T., Fujita K., Hayashi K., Honjo A., Ikemoto K., Inada T., Itoh T., Kajihara M., Kanai K., Kashimoto K., Kimura S., Kitagawa M., Makino K., Masuda S., Miki T., Mizobuchi K., Mori H., Motomura K., Nakamura Y., Nashimoto H., Nishio Y., Saito N., Sampei G., Seki Y., Tagami H., Takemoto K., Wada C., Yamamoto Y., Yano M., Horiuchi T.;
"A 718-kb DNA sequence of the Escherichia coli K-12 genome corresponding to the 12.7-28.0 min region on the linkage map.";
DNA Res. 3:137-155(1996).
[3]
NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
STRAIN=K12 / MG1655 / ATCC 47076;
DOI=10.1126/science.277.5331.1453; PubMed=9278503 [NCBI, ExPASy, EBI, Israel, Japan]
Blattner F.R., Plunkett G. III, Bloch C.A., Perna N.T., Burland V., Riley M., Collado-Vides J., Glasner J.D., Rode C.K., Mayhew G.F., Gregor J., Davis N.W., Kirkpatrick H.A., Goeden M.A., Rose D.J., Mau B., Shao Y.;
"The complete genome sequence of Escherichia coli K-12.";
Science 277:1453-1474(1997).
[4]
NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
STRAIN=K12 / W3110 / ATCC 27325 / DSM 5911;
DOI=10.1038/msb4100049; PubMed=16738553 [NCBI, ExPASy, EBI, Israel, Japan]
Hayashi K., Morooka N., Yamamoto Y., Fujita K., Isono K., Choi S., Ohtsubo E., Baba T., Wanner B.L., Mori H., Horiuchi T.;
"Highly accurate genome sequences of Escherichia coli K-12 strains MG1655 and W3110.";
Mol. Syst. Biol. 2:E1-E5(2006).
[5]
FUNCTION.
DOI=10.1021/bi00391a039; PubMed=2823881 [NCBI, ExPASy, EBI, Israel, Japan]
Bagg A., Neilands J.B.;
"Ferric uptake regulation protein acts as a repressor, employing iron (II) as a cofactor to bind the operator of an iron transport operon in Escherichia coli.";
Biochemistry 26:5471-5477(1987).
[6]
STRUCTURAL DYNAMICS.
DOI=10.1021/bi00247a016; PubMed=1868094 [NCBI, ExPASy, EBI, Israel, Japan]
Coy M., Neilands J.B.;
"Structural dynamics and functional domains of the fur protein.";
Biochemistry 30:8201-8210(1991).
[7]
ZINC-BINDING SITES.
DOI=10.1021/bi9902283; PubMed=10387106 [NCBI, ExPASy, EBI, Israel, Japan]
Gonzalez de Peredo A., Saint-Pierre C., Adrait A., Jacquamet L., Latour J.M., Michaud-Soret I., Forest E.;
"Identification of the two zinc-bound cysteines in the ferric uptake regulation protein from Escherichia coli: chemical modification and mass spectrometry analysis.";
Biochemistry 38:8582-8589(1999).
[8]
STRUCTURE BY NMR.
PubMed=2015825 [NCBI, ExPASy, EBI, Israel, Japan]
Saito T., Williams R.J.;
"The binding of the ferric uptake regulation protein to a DNA fragment.";
Eur. J. Biochem. 197:43-47(1991).
Comments
Copyright
Copyrighted by the UniProt Consortium, see http://www.uniprot.org/terms. Distributed under the Creative Commons Attribution-NoDerivs License.
Cross-references
Sequence databases
EMBL
X02589; CAA26429.1; -; Genomic_DNA.[EMBL / GenBank / DDBJ] [CoDingSequence]
U00096; AAC73777.1; -; Genomic_DNA.[EMBL / GenBank / DDBJ] [CoDingSequence]
AP009048; BAA35331.1; -; Genomic_DNA.[EMBL / GenBank / DDBJ] [CoDingSequence]
PIR S07308; S07308.
RefSeq AP_001321.1; -.
NP_415209.1; -.
3D structure databases
PDB
2FU4; X-ray; 1.80 A; A/B=1-83.[ExPASy / RCSB / EBI]
PDBsum 2FU4; -.
ModBase P0A9A9.
Protein-protein interaction databases
IntAct P0A9A9; -.
Enzyme and pathway databases
BioCyc EcoCyc:PD00260; -.
2D gel databases
SWISS-2DPAGE P0A9A9; -.
ECO2DBASE G015.8; 6TH EDITION.
Organism-specific databases
EchoBASE EB0354; -.
EcoGene EG10359; fur.
Ontologies
GO
GO:0005515; Molecular function: protein binding (inferred from physical interaction from IntAct).
QuickGo view.
Family and domain databases
InterPro IPR002481; FUR.
Graphical view of domain structure.
Pfam PF01475; FUR; 1.
Pfam graphical view of domain structure.
ProDom PD002003; FUR; 1.
[Domain structure / List of seq. sharing at least 1 domain]
BLOCKS P0A9A9.
Genome annotation databases
GeneID 945295; -.
GenomeReviews U00096_GR; b0683.
AP009048_GR; JW0669.
KEGG ecj:JW0669; -.
eco:b0683; -.
Phylogenomic databases
HOGENOM P0A9A9; -.
Genome annotation databases
CMR P0A9A9; b0683.
Other
ProtoNet P0A9A9.
UniRef View cluster of proteins with at least 50% / 90% / 100% identity.
Keywords
3D-structure; Complete proteome; Cytoplasm; DNA-binding; Iron; Metal-binding; Repressor; Transcription; Transcription regulation; Zinc.
Features
SEVIEWER logo Feature table viewer
KeyFrom  To Length Description FTId
CHAIN   1   148  148     Ferric uptake regulation protein. PRO_0000095550
COMPBIAS   86    90  5     His-rich. 
METAL   93    93        Zinc. 
METAL   96    96        Zinc. 
HELIX   4    10  7      
HELIX   17    26  10      
HELIX   29    31  3      
STRAND   32    34  3      
HELIX   36    45  10      
HELIX   52    64  13      
STRAND   67    72  6      
HELIX   74    76  3      
STRAND   78    82  5      
Sequence information
Length: 148 AA [This is the length of the unprocessed precursor] Molecular weight: 16795 Da [This is the MW of the unprocessed precursor] CRC64: B868360513489DD3 [This is a checksum on the sequence]
        10         20         30         40         50         60 
MTDNNTALKK AGLKVTLPRL KILEVLQEPD NHHVSAEDLY KRLIDMGEEI GLATVYRVLN 

        70         80         90        100        110        120 
QFDDAGIVTR HNFEGGKSVF ELTQQHHHDH LICLDCGKVI EFSDDSIEAR QREIAAKHGI 

       130        140 
RLTNHSLYLY GHCAEGDCRE DEHAHEGK 

P0A9A9 in FASTA format

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