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UniProtKB/Swiss-Prot entry P0A6V8

[Entry info] [Name and origin] [References] [Comments] [Cross-references] [Keywords] [Features] [Sequence] [Tools]

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Entry information
Entry name GLK_ECOLI
Primary accession number P0A6V8
Secondary accession numbers P46880 P78276
Integrated into Swiss-Prot on March 29, 2005
Sequence was last modified on March 29, 2005 (Sequence version 1)
Annotations were last modified on    December 16, 2008 (Entry version 36)
Name and origin of the protein
Protein name Glucokinase
Synonyms EC 2.7.1.2
Glucose kinase
Gene name
Name: glk
OrderedLocusNames: b2388, JW2385
From
Escherichia coli (strain K12) [TaxID: 83333] [HAMAP proteome]
Taxonomy Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacteriales; Enterobacteriaceae; Escherichia.
Protein existence 1: Evidence at protein level;
References
[1]
 NUCLEOTIDE SEQUENCE [GENOMIC DNA], AND CHARACTERIZATION.
STRAIN=K12;
PubMed=9023215 [NCBI, ExPASy, EBI, Israel, Japan]
Meyer D., Schneider-Fresenius C., Horlacher R., Peist R., Boos W.W.;
"Molecular characterization of glucokinase from Escherichia coli K-12.";
J. Bacteriol. 179:1298-1306(1997).
[2]
 NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
STRAIN=K12 / W3110 / ATCC 27325 / DSM 5911;
DOI=10.1093/dnares/4.2.91; PubMed=9205837 [NCBI, ExPASy, EBI, Israel, Japan]
Yamamoto Y., Aiba H., Baba T., Hayashi K., Inada T., Isono K., Itoh T., Kimura S., Kitagawa M., Makino K., Miki T., Mitsuhashi N., Mizobuchi K., Mori H., Nakade S., Nakamura Y., Nashimoto H., Oshima T., Oyama S., Saito N., Sampei G., Satoh Y., Sivasundaram S., Tagami H., Takahashi H., Takeda J., Takemoto K., Uehara K., Wada C., Yamagata S., Horiuchi T.;
"Construction of a contiguous 874-kb sequence of the Escherichia coli-K12 genome corresponding to 50.0-68.8 min on the linkage map and analysis of its sequence features.";
DNA Res. 4:91-113(1997).
[3]
 NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
STRAIN=K12 / MG1655 / ATCC 47076;
DOI=10.1126/science.277.5331.1453; PubMed=9278503 [NCBI, ExPASy, EBI, Israel, Japan]
Blattner F.R., Plunkett G. III, Bloch C.A., Perna N.T., Burland V., Riley M., Collado-Vides J., Glasner J.D., Rode C.K., Mayhew G.F., Gregor J., Davis N.W., Kirkpatrick H.A., Goeden M.A., Rose D.J., Mau B., Shao Y.;
"The complete genome sequence of Escherichia coli K-12.";
Science 277:1453-1474(1997).
[4]
 NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
STRAIN=K12 / W3110 / ATCC 27325 / DSM 5911;
DOI=10.1038/msb4100049; PubMed=16738553 [NCBI, ExPASy, EBI, Israel, Japan]
Hayashi K., Morooka N., Yamamoto Y., Fujita K., Isono K., Choi S., Ohtsubo E., Baba T., Wanner B.L., Mori H., Horiuchi T.;
"Highly accurate genome sequences of Escherichia coli K-12 strains MG1655 and W3110.";
Mol. Syst. Biol. 2:E1-E5(2006).
Comments
Copyright
Copyrighted by the UniProt Consortium, see http://www.uniprot.org/terms. Distributed under the Creative Commons Attribution-NoDerivs License.
Cross-references
Sequence databases
EMBL
U22490; AAA64506.1; -; Genomic_DNA.[EMBL / GenBank / DDBJ] [CoDingSequence]
U00096; AAC75447.1; -; Genomic_DNA.[EMBL / GenBank / DDBJ] [CoDingSequence]
AP009048; BAA16258.1; -; Genomic_DNA.[EMBL / GenBank / DDBJ] [CoDingSequence]
PIR A65013; A65013.
RefSeq AP_002988.1; -.
NP_416889.1; -.
3D structure databases
PDB
1Q18; X-ray; 2.36 A; A/B=2-321.[ExPASy / RCSB / EBI]
1SZ2; X-ray; 2.20 A; A/B=2-321.[ExPASy / RCSB / EBI]
Detailed list of linked structures.
PDBsum 1Q18; -.
1SZ2; -.
ModBase P0A6V8.
Enzyme and pathway databases
BioCyc EcoCyc:GLUCOKIN-MON; -.
MetaCyc:GLUCOKIN-MON; -.
Organism-specific databases
EchoBASE EB2791; -.
EcoGene EG12957; glk.
Ontologies
GO
GO:0005737; Cellular component: cytoplasm (inferred from electronic annotation from HAMAP).
GO:0005524; Molecular function: ATP binding (inferred from electronic annotation from HAMAP).
GO:0004340; Molecular function: glucokinase activity (inferred from electronic annotation from HAMAP).
GO:0006096; Biological process: glycolysis (inferred from electronic annotation from HAMAP).
QuickGo view.
Family and domain databases
HAMAP MF_00524; -; 1.
PBIL [Tree]
InterPro IPR003836; Glucokinase.
Graphical view of domain structure.
Pfam PF02685; Glucokinase; 1.
Pfam graphical view of domain structure.
TIGRFAMs TIGR00749; glk; 1.
Genome annotation databases
GeneID 946858; -.
GenomeReviews AP009048_GR; JW2385.
U00096_GR; b2388.
KEGG ecj:JW2385; -.
eco:b2388; -.
Phylogenomic databases
HOGENOM P0A6V8; -.
Genome annotation databases
CMR P0A6V8; b2388.
Other
ProtoNet P0A6V8.
UniRef View cluster of proteins with at least 50% / 90% / 100% identity.
Keywords
3D-structure; ATP-binding; Complete proteome; Cytoplasm; Glycolysis; Kinase; Nucleotide-binding; Transferase.
Features
SEVIEWER logo Feature table viewer
KeyFrom   To Length Description FTId
CHAIN   1   321  321     Glucokinase. PRO_0000215124
NP_BIND   8    13  6     ATP (Potential). 
CONFLICT   108   108        I -> N (in Ref. 1; AAA64506). 
CONFLICT   188   189        RV -> AC (in Ref. 1; AAA64506). 
CONFLICT   274   274        A -> G (in Ref. 1; AAA64506). 
STRAND   4    10  7      
STRAND   12    21  10      
TURN   22    24  3      
STRAND   27    34  8      
HELIX   35    37  3      
HELIX   41    52  12      
STRAND   57    65  9      
STRAND   69    72  4      
HELIX   77    79  3      
HELIX   83    89  7      
STRAND   93    99  7      
HELIX   100   107  8      
HELIX   108   110  3      
HELIX   113   115  3      
STRAND   116   118  3      
STRAND   130   147  18      
STRAND   150   154  5      
HELIX   158   160  3      
HELIX   168   180  13      
TURN   187   189  3      
HELIX   192   205  14      
HELIX   215   223  9      
HELIX   228   252  25      
STRAND   258   260  3      
HELIX   265   268  4      
HELIX   269   273  5      
HELIX   277   282  6      
HELIX   285   287  3      
HELIX   288   291  4      
STRAND   296   299  4      
HELIX   304   316  13      
Sequence information
Length: 321 AA [This is the length of the unprocessed precursor] Molecular weight: 34723 Da [This is the MW of the unprocessed precursor] CRC64: 2D6CD956641A3823 [This is a checksum on the sequence] 
        10         20         30         40         50         60 
MTKYALVGDV GGTNARLALC DIASGEISQA KTYSGLDYPS LEAVIRVYLE EHKVEVKDGC 

        70         80         90        100        110        120 
IAIACPITGD WVAMTNHTWA FSIAEMKKNL GFSHLEIIND FTAVSMAIPM LKKEHLIQFG 

       130        140        150        160        170        180 
GAEPVEGKPI AVYGAGTGLG VAHLVHVDKR WVSLPGEGGH VDFAPNSEEE AIILEILRAE 

       190        200        210        220        230        240 
IGHVSAERVL SGPGLVNLYR AIVKADNRLP ENLKPKDITE RALADSCTDC RRALSLFCVI 

       250        260        270        280        290        300 
MGRFGGNLAL NLGTFGGVFI AGGIVPRFLE FFKASGFRAA FEDKGRFKEY VHDIPVYLIV 

       310        320 
HDNPGLLGSG AHLRQTLGHI L
P0A6V8 in FASTA format

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