FUNCTION: Involved in cell wall formation. Catalyzes the final step in the synthesis of UDP-N-acetylmuramoyl-pentapeptide, the precursor of murein (By similarity).
CATALYTIC ACTIVITY: ATP + UDP-N-acetylmuramoyl-L-alanyl-gamma-D-glutamyl-L-lysine + D-alanyl-D-alanine = ADP + phosphate + UDP-N-acetylmuramoyl-L-alanyl-gamma-D-glutamyl-L-lysyl-D-alanyl-D-alanine.
PATHWAY: Cell wall biogenesis; peptidoglycan biosynthesis .
SUBUNIT: Monomer (By similarity).
SUBCELLULAR LOCATION: Cytoplasm (Probable).
SIMILARITY: Belongs to the murCDEF family.

Copyright

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Cross-references

Sequence databases

EMBL

BX248341; CAD97034.1; -; Genomic_DNA.

[EMBL / GenBank / DDBJ] [CoDingSequence]

RefSeq

NP_855830.1; -.

3D structure databases

HSSP

P11880; 1GG4. [HSSP ENTRY / PDB]

ModBase

P0A5L5.

Ontologies

GO:0005737;	Cellular component: cytoplasm (inferred from electronic annotation from InterPro).
GO:0005524;	Molecular function: ATP binding (inferred from electronic annotation from InterPro).
GO:0047480;	Molecular function: UDP-N-acetylmuramoyl-tripeptide-D-alanyl-D-alanine ligase activity (inferred from electronic annotation from EC).
GO:0008766;	Molecular function: UDP-N-acetylmuramoylalanyl-D-glutamyl-2,6-diaminopimelate-D-alanyl-D-alanine ligase activity (inferred from electronic annotation from InterPro).
GO:0007049;	Biological process: cell cycle (inferred from electronic annotation from UniProtKB-KW).
GO:0051301;	Biological process: cell division (inferred from electronic annotation from InterPro).
GO:0009252;	Biological process: peptidoglycan biosynthetic process (inferred from electronic annotation from InterPro).
GO:0008360;	Biological process: regulation of cell shape (inferred from electronic annotation from InterPro).
QuickGo view.

Family and domain databases

InterPro

IPR004101; Mur_ligase_C.
IPR013221; Mur_ligase_cen.
IPR000713; Mur_ligase_N.
IPR005863; UDP-N-AcMur-3pep_AlaAla_ligase.
Graphical view of domain structure.

Gene3D

G3DSA:3.90.190.20; Mur_ligase_C; 1.
G3DSA:3.40.1190.10; Mur_ligase_cen; 1.

PANTHER

PTHR23135:SF3; MurF; 1.

Pfam

PF01225; Mur_ligase; 1.
PF02875; Mur_ligase_C; 1.
PF08245; Mur_ligase_M; 1.
Pfam graphical view of domain structure.

TIGRFAMs

TIGR01143; murF; 1.

Genome annotation databases

GeneID

1091261; -.

GenomeReviews

BX248333_GR; Mb2181c.

KEGG

mbo:Mb2181c; -.

Phylogenomic databases

HOGENOM

P0A5L5; -.

Genome annotation databases

CMR

P0A5L5; Mb2181c.

Other

ProtoNet

P0A5L5.

UniRef

View cluster of proteins with at least 50% / 90% / 100% identity.

Keywords

ATP-binding; Cell cycle; Cell division; Cell shape; Cell wall biogenesis/degradation; Complete proteome; Cytoplasm; Ligase; Nucleotide-binding; Peptidoglycan synthesis.

Features

Feature table viewer

`Key`	`From To`	`Length`	`Description`	`FTId`
`CHAIN`	`1 510`	`510`	`UDP-N-acetylmuramoyl-tripeptide--D-alanyl-D-alanine ligase.`	`PRO_0000101702`
`NP_BIND`	`136 142`	`7`	`ATP (Potential).`

Sequence information

Length: 510 AA [This is the length of the unprocessed precursor]

Molecular weight: 51633 Da [This is the MW of the unprocessed precursor]

CRC64: 7BE83EDD1BAA2EC4 [This is a checksum on the sequence]

        10         20         30         40         50         60 
MIELTVAQIA EIVGGAVADI SPQDAAHRRV TGTVEFDSRA IGPGGLFLAL PGARADGHDH 

        70         80         90        100        110        120 
AASAVAAGAA VVLAARPVGV PAIVVPPVAA PNVLAGVLEH DNDGSGAAVL AALAKLATAV 

       130        140        150        160        170        180 
AAQLVAGGLT IIGITGSSGK TSTKDLMAAV LAPLGEVVAP PGSFNNELGH PWTVLRATRR 

       190        200        210        220        230        240 
TDYLILEMAA RHHGNIAALA EIAPPSIGVV LNVGTAHLGE FGSREVIAQT KAELPQAVPH 

       250        260        270        280        290        300 
SGAVVLNADD PAVAAMAKLT AARVVRVSRD NTGDVWAGPV SLDELARPRF TLHAHDAQAE 

       310        320        330        340        350        360 
VRLGVCGDHQ VTNALCAAAV ALECGASVEQ VAAALTAAPP VSRHRMQVTT RGDGVTVIDD 

       370        380        390        400        410        420 
AYNANPDSMR AGLQALAWIA HQPEATRRSW AVLGEMAELG EDAIAEHDRI GRLAVRLDVS 

       430        440        450        460        470        480 
RLVVVGTGRS ISAMHHGAVL EGAWGSGEAT ADHGADRTAV NVADGDAALA LLRAELRPGD 

       490        500        510 
VVLVKASNAA GLGAVADALV ADDTCGSVRP

P0A5L5 in FASTA format

View entry in original UniProtKB/Swiss-Prot format
View entry in raw text format (no links)
Report form for errors/updates in this UniProtKB/Swiss-Prot entry

	BLAST submission on ExPASy/SIB or at NCBI (USA)		Sequence analysis tools: ProtParam, ProtScale, Compute pI/Mw, PeptideMass, PeptideCutter, Dotlet (Java)
	ScanProsite, MotifScan		Submit a homology modeling request to SWISS-MODEL
	NPSA Sequence analysis tools